675 Matching Annotations
  1. Jul 2019
    1. Photography, evaluation and documentation
    2. Procedurefor SDS-PAGE
    3. Materialsrequired for SDS-PAGE
    4. Effect of temperature on xylanase activity
    5. Effect of pH on xylanase activity
    6. Effect of various carbon sourceson xylanase activity
    7. Assayof xylanase activity
    8. Dinitrosalicylate reagent (DNS)(per liter)
    9. Citrate phosphate buffer
    10. Reagents
    11. Xylanase activity
    12. Harvesting of cultures
    13. Enzyme production (EP) medium
    14. Inoculum preparation
    15. Sporulation medium used for Trichodermasp
    16. Maintenance of Trichodermasp. culture
    17. Sterilization
    18. Materials for xylanase induction
    19. Induction of xylanase from Trichodermasp
    20. Evaluation of bioagents against the pathogen
    21. Laboratory screening of antagonists against the test pa
    22. Effect of pHon growth and sporulation of the bioagent
    23. Effect of temperature on growth of bioagent
    24. Effect of different media on growth of bioagentTrichoderma
    25. Cultural characteristicsof bioagent Trichoderma
    26. Mycelial characteristicsof pathogen
    27. Identificationof bioagent
    28. Isolation and purification of Trichoderma sp.
    29. Isolation and purification of pathogen, Fusarium udum
    30. Isolation, purification and morphological characterization of pathogen and bioagent
    31. Symptomatology
    32. Collection of the diseased materia
    33. Sterilization of laminar air flow
    34. Sterilization of media and distilled water
    35. Sterilization of glasswares
    36. Sterilization procedure
    37. Source of chemicals
    38. Experimental site
    1. Gel documentation and image analysis
    2. Screening for virulence genes in V. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood
    3. Statistical analysis
    4. Detection of hemolytic activity
    5. Production of caseinase
    6. Production of phosphatase
    7. Bacterial strains used
    8. DNAisolation
    9. Detection of virulence genes tdhand trhby multiplex
    10. Detection of type III secretion system genes
    11. Production of chitinase
    12. Production of DNas
    13. Production of gelatinase
    14. Production of Lipase
    15. Production of amylase
    16. Bacterial strains used
    17. Screening of Vibriostrains for extracellular enzymes
    1. Statistical analysis
    2. Gel documentation and image analysis
    3. Detection of toxR gene
    4. Detection of tlhgene
    5. Extraction of genomic DNA
    6. Detection of V. parahaemolyticusspecies-specific gene
    7. Isolation of V. parahaemolyticuson HiCrome Vibrio
    8. Isolation and identification of V. parahaemolyticus
    9. PCR amplification of 16S rRNA gene
    10. DNA isolatio
    11. Molecular confirmation of Vibrioby 16S rRNA gene se
    12. Resistance to ampicillin
    13. Citrate utilisation tes
    14. Nitrate reduction test
    15. Production of β-galactosidase (ortho-Nitrophenyl β-D-galactopyranoside (ONPG)) test
    16. Urease production
    17. Gelatinase production
    18. Growth at different temperatures
    19. Salt tolerance tes
    20. Voges Proskauer (acetoin production) test
    21. Indole production
    22. Carbon source utilisation tes
    23. Carbohydrate fermentation test
    24. Amino acids utilisation test (Decarboxylase/dihydrol
    25. Species level identification
    26. Oxidative-Fermentative test
    27. Oxidase test
    28. Gram staining
    29. Presumptive identification
    30. Isolation of Vibriospecies from water and sediment of Cochin estuary
    31. Sample collection
    32. Analysis of hydrographical parameters
    33. Description of sampling site
    34. Molecular confirmation of Vibrioby 16S rRNA gene s
    35. Resistance to ampicillin
    36. Citrate utilisation tes
    37. Nitrate reduction tes
    38. Production of β-galactosidase (ortho-Nitrophenyl β-D-galactopyranoside (ONPG)) test
    39. Urease productio
    40. Gelatinase productio
    41. Growth at different temperature
    42. Salt tolerance tes
    43. Voges Proskauer (acetoin production) tes
    44. Indole production
    45. Carbon source utilisation t
    46. Carbohydrate fermentation tes
    47. Amino acids utilisation test (Decarboxylase/dihydrola
    48. Species level identification
    49. Oxidative-Fermentative test
    50. Oxidase tes
    51. Gram staining
    52. Presumptive identification
    53. Isolation of Vibriospecies from water and sediment of Cochin estuary
    54. Sample collection
    55. Analysis of hydrographical parameters
    56. Description of sampling site
  2. Jun 2019
    1. RNA Interference
    2. Cell transfection
    3. Secondary antibodies
    4. Primary antibodies
    5. Antibodies used in our study
    6. Cell culture
    7. General reagents and plastic wares
    8. Medium and Serum
    9. Animals
    10. pGEX6P2-BPN (1-993 a.a)
    11. pET30a-GFP-FL (GFP antigen)
    12. pET30-b-APC Nde/Sca (1211-1495 a.a) (antigen for APC antibody)
    13. pET30a-β-catenin-FL (Antigen for -catenin antibody)
    14. GFP-β-cateninC-C (423-634 a.a) (ARM-C)
    15. GFP-β-catenin-C-N (151-423 a.a) (ARM-N)
    16. GFP-β-catenin-C (ARM)
    17. GFP-β-catenin- C (634 -781 a.a)
    18. GFP-β-catenin- a.a
    19. GFP-β-catenin T41A mutant
    20. Constructs made in the study
    21. GFP-β-catenin-FL