1. Huitzilopochtli is first in rank, no one, no one is like unto him: not vainly do I sing (his praises) coming forth in the garb of our ancestors; I shine; I glitter. 2. He is a terror to the Mixteca; he alone dest
many of the hymns are about how fear the gods are
Germination of N.sativaseeds
In vitro cytotoxic activity of methanolic extract of N. sativafrom different germination phasesduringSRB assay
Cytotoxicity assay by Sulphorhodamine B (SRB) me
Germination of N. sativaseeds
Activation of TLC plate
Tests for Alkaloids
Germination of N.sativaseeds
Testing of bioformulations
Sequence analysis
DNA sequencing of the 18S rDNA fragment
Purification of PCR product
Analysis of internal transcribed spacer region
RAPDand SSRscoring and data analysis
PCR amplification
Running of gel and visualization of DNA
Determination of the yield
Agarose gel electrophoresis
Qualitative and quantitative estimation of DNA
Determination of the yield
Procedure for DNA isolation
Reagents required for fungal DNA isolationand p
DNA isolation of Trichodermaisolate
Genetic variability analysis through RAPD and SSR
Photography, evaluation and documentation
Procedurefor SDS-PAGE
Materialsrequired for SDS-PAGE
Protein profiling of bioagent through SDS-PAGE
Assayof xylanase activity
Dinitrosalicylate reagent (DNS)(per liter)
Sterilization
Effect of temperature on growth of bioagent
Identificationof bioagent
Isolation and purification of Trichoderma sp.
Sterilization of media and distilled water
Source of chemicals
Prevalence of extracellular virulence factors in Vibriofrom shrimp farm
Gel documentation and image analysis
Screening for virulence genes in V. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood
Bacterial strains used
DNAisolation
Detection of virulence genes tdhand trhby multiplex
Detection of type III secretion system genes
Production of Lipase
MAR indexingand antibiotic resistance pattern amon
Antibiotic resistance amongVibriofrom shrimp farm
Detection of blaTEMgene
MAR indexing
Antibiotic resistance amongVibriofrom Cochin estu
Species level identification and distribution of Vibriospecies in Cochin estuary
Gel documentation and image analysis
Detection of toxR gene
Detection of tlhgene
Extraction of genomic DNA
Detection of V. parahaemolyticusspecies-specific gene
PCR amplification of 16S rRNA gene
Carbohydrate fermentation test
Oxidase test
Analysis of hydrographical parameters
Carbohydrate fermentation tes
Oxidase tes
Analysis of hydrographical parameters
Active site identification, metal detection and interaction of Dof domain structure
Superposition of the Dof domain with predicted 3D structure
Validation of the predicted 3D structure
Tertiary structural prediction
Chromosomal locations
Phylogenetic and motif analysi
In silico characterization of cloned Dof gene
Phylogenetic and motif analysis of sequenced Dof domains
In silico characterization of sequenced Dof domains of cereal
Sequencing of Dof domain and gene
Cloning of Dof genes of sorghum using pBSK vector
Cloning of Dof domain and Dof genes using pGEM-T Easy
Gel elution of PCR products
PCR based cloning, sequencing and in silico characterization of Dof domain and Dofgenes of cereals and millet
PCR amplification of Dof gene
Primer designing for PCR amplification of Dof domain and Do
Active site prediction and docking study
Superposition of predicted structure and template (Dof
Validations
Three dimensional structure prediction, refinements and evaluation of Dof proteins
Cis-regulatory element analysis
Mapping of SbDof genes on sorghum chromosomes and its intron/exon gene structure prediction
In silico prediction of Dof gene family members in S. bicolor (L
Motif identification
Multiple sequence alignment and phylogenetic analysis
In silico analysis of sequenced Dof domain and Dof genes
Reaction resuspension
Post-reaction clean up
Sequencing PCR
Sequencing reaction
Digestion of Plasmid DNA
Minipreparation of plasmid DNA from transformed co
Screening of recombinant E. coli clone
Transformation of ligation mixture in electro-competent E. coli host cells (DH5ααααstrain)
Transformation of ligated product in chemically competent E. coli host cells (DH5αααα strain)
Ligation reactions
Dephosphorylation of vector
Restriction digestion
Ligation of eluted PCR product in pBSK vector
Cloning of gel eluted PCR produc
Gel elution of PCR Produ
Scoring of amplification data points and construction of a den
Analysis of PCR amplicons using agarose gel electrophoresis
Cycling condition
PCR reaction set up
Basic requirements for PC
PCR amplification of Dof domain and Dof genes from different
Primer designing for PCR amplification of Dof domain and D
Qualitative analysis of DNA by agarose gel electrophoresi
Spectrophotometric quantification of genomic DNA
DNA purification
DNA extraction procedure
Isolation of genomic DNA by CTAB method
Glasswares, plasticwares and equipments
Immunological Responses (DTH, mitogenic and Leishmania-specific cellular responses)
Formaldehyde gel electrophoresis
LTT assay
2 Anim
Nitrite production in macrophages of hamsters
Animals
rLdTPR was cloned, overexpressed, purified and antibody rais
Overexpression and Purification of recombinant protein (rLdTP
Clone confirmation
Colony PCR
Transformation
PCR amplification
2D QSAR
Active site analysis
Inhibitors Dataset
Assay for Lipid peroxidase(LPO)
Cytomorphological analysis
Growth kinetic studies
Reducing power assay
TEM-EDAX analysis
Qualitative analysis of compounds
The table 6.1 gives the mixing probabilities and the associated parametricvalues fork(number of components) = 2,3, and 4. It may be noted thatthe Log likelihood value is smaller fork= 4 (the results fork= 5 , 6 etc.are not better than that fork= 4 and hence are not given here). The fourcomponents Poisson Mixture model is given in table 6.2. It may be notedthat 58% of wards may have higher incidence/relative risk and the remainingwards have lesser/lower incidence for the Cancer disease. We computed theposterior probability for each component for each ward (see table 6.3). Eachward is assigned to a particular component so that the posterior probability islarger. These results are also given in table 6.3 Finally we present Choroplethmaps based on those results
The Posterior Probability of Mixing Dis-tribution
Algorithm
EM Procedure
Poisson Mixture
Data Sources
Poisson Mixtures Distribution
Poisson Model
Haemolymph protein profile
Helicoverpa armiger
Spodoptera litura
Gut enzyme profile
Haemolymph protein profiling
Lactate dehydrogenase
VS preparation
Utilization of VS by the prey
Influence of prey on VS yield
Quantification of protein of VS yielded in the prey deprivation
Survival (SR) and venom milking rate (VMR
Venomous saliva optimization
Statistical analysi
Quantification of VS for protein
Venomous saliva utilization
Prey type
Starvation and collection method
Venomous saliva optimization
Insects Collection
VENOMOUS SALIVA: OPTIMIZATION AND UTILIZATION
Mandibular stylet
Head and stylet preparation
Micronuclei test
Malic dehydrogenase
Alkaline Phosphatase
Glycogen content
Analysis of Physico-chemical parameters of the water sampled
Increments in biomass:
Residual nutrients
Chlorophyll content in waters:
Growth maximum values
Rates of growth (OD678/day
Growth
Experimental Set up
BIOMASS PRODUCTION: ROLE OF N-P CONCENTRATION AND RATIOS
Soil pH and electrical conductivity (EC)
Experiment 2: Assessing the impacts of elevated temperature and N levels on yield and nutrient uptake in rice
Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice
Experimental Design and Treatments
Temperature Gradient Tunnels (TGT)
Yield and its component traits:
Differences among cultivars for yield, biomass and HI
Kernel hardness
SDS-sedimentation test
Estimation of total N% of wheat grainsand straw
End use Quality parameters
Chlorophyllcontent
Root length (cm) and Root weight (mg)
Coleoptile length(cm)
Stomata / cm2
Leaf area index (LAI)
Physiological parameters
Laboratory observations
Days to physiological maturity
Static and shaken condition