- Apr 2017
while those that had been moved to 25 degrees had largely lost the methylation tags. Importantly, they still maintained this reduced histone methylation when moved back to the cooler temperature, suggesting that it is playing an important role in locking the memory into the transgenes.
Could it be that the genetic background (or importantly surrounding environment or cross talk among cells) in the first generation that were exposed to 25 C temp might have changed, and conditioned subsequent generations?
After 14 generations, secondary changes in the propoagated genetic makeup (including cross talk) could have reverted phenotype and reset histone marks to repress gene expression.
See if how often c.elegans mutate or show CN changes, i.e., background vs stress induced by temp changes?