strong promoters capable of driving expression of microbial opsins or fluorescent proteins in specific populations can exhibit leaky expression elsewhere. This low-level leak may be virtually undetectable as light responsiveness or fluorescence but can be a serious issue when expressing Cre recombinase.

In soil, bacteria tend to live in a state of dormancy due to prevailing oligotrophic conditions, which would not be particularly favorable for the development of competence

bacteria generally express genes only when and where needed, and thus do not readily reveal their pathogenic armament outside of infected tissues

regulate gene expression, typically by occluding or exposing regulatory features, such as ribosome binding sites (RBSs) in the case of translation or intrinsic terminator hairpins in the case of transcription

In culture conditions that enhance cell-to-cell contact, conjugation rates approach 100% with the cis-acting plasmid

it can be useful for reducing leaky expression of toxic proteins

We report the construction of a family of vectors that contain a reengineered lacIq-lac promoter-operator complex in which cloned genes are strongly repressed in the absence of inducer.

While these techniques offer good repression, they exhibit leakiness that precludes the gene of interest from being completely turned off

absence of nitrogen was found to increase HGT events three orders of magnitude

We have previously shown that antinociceptive effects of morphine are enhanced in histamine H1 receptor gene knockout mice.

n amalgam of amphibian neural webbing andsynthetic DNA

low rates of indels (typically no more than 0.1%

Massive mining of publicly available RNA-seq data from human and mouse

Gene ID

repeat expansion at IIL1 leads to increased accumulation of 24-nt siRNAs in a temperature-dependent manner that correlates with the iil phenotype. We show that DCL3 and other components of the RNA-dependent DNA methylation (RdDM) pathway are essential for this siRNA-directed epigenetic gene silencing

RNA-seq analysis is easy as 1-2-3 with limma, Glimma and edgeR [version 2; referees: 3 approved]

Robust enumeration of cell subsets from tissue expression profiles

Gene Set Enrichment Analysis Made Simple

Massive Mining of Publicly Available RNA-seq Data from Human and Mouse

The ISMARA client

ISMARA: automated modeling of genomic signals as a democracy of regulatory motifs

basic

blast