37 Matching Annotations
  1. Last 7 days
    1. Consequently, the plasmid donor cells do not express gfp

      Does the leaky expression not hinder the detecting and quantification of transconjugants in epifluorescence microscopy?

  2. Jan 2020
    1. a weapon — say by sabotaging the pollinators that support agriculture, or by altering the genes of innocuous wild insects so they could transmit disease
    2. Could a gene drive stop one virus only to open the way for another, more virulent one? Could it jump from one species to a related one? What would be the environmental effects, if any, of altering the genes of entire species? How about eliminating a species entirely?
    3. Besides combating malaria, gene drives could be used to alter, or even eliminate, other disease-causing insects, from the sand flies that transmit leishmaniasis to ticks that carry Lyme disease in the United States.
    1. use of the strong UAAU signal in highly expressed genes and for the occurrence of the weaker UGAC signal at several recording sites.

      three types of recoding events: stop-codon readthrough, –1 ribosome frameshifting and translational bypassing

      (Rodnina, Marina V., et al. "Translational recoding: canonical translation mechanisms reinterpreted." Nucleic acids research (2019).)

    1. suggestive of protein extension, especially at UGA codons, which rely exclusively on the reduced function RF2 variant of the K-12 strain for termination
  3. Nov 2019
    1. HGT typically adds new catabolic routes to microbial metabolic networks. This increases the chance of new metabolic interactions between bacteria
  4. Oct 2019
    1. We tested a broad range of Enterobacteriaceae conjugative plasmids for their sensitivity to the growth stage of the donors and identified three distinct regulation types.
    1. We have demonstrated that small RNAs can tightly repress their target genes when their synthesis rate is smaller than some threshold, but have little or no effect when the synthesis rate is much larger than that threshold
    2. a threshold-linear response with a tunable threshold, a robust noise resistance characteristic, and a built-in capability for hierarchical cross-talk.
    1. In particular, small RNAs are shown to establish a threshold for the expression of their target, providing safety mechanism against random fluctuations and transient signals. The threshold level is set by the transcription rate of the small RNA and can thus be modulated dynamically to reflect changing environmental conditions.
    1. in the absence of tamoxifen, it exhibits some activity
    2. strong promoters capable of driving expression of microbial opsins or fluorescent proteins in specific populations can exhibit leaky expression elsewhere. This low-level leak may be virtually undetectable as light responsiveness or fluorescence but can be a serious issue when expressing Cre recombinase.
    1. In soil, bacteria tend to live in a state of dormancy due to prevailing oligotrophic conditions, which would not be particularly favorable for the development of competence

      It can also be argued that the transformed DNA is a good resource of phosphates, nitrogen etc., so might be favoured in oligotrophic conditions?

  5. sackler.tufts.edu sackler.tufts.edu
    1. bacteria generally express genes only when and where needed, and thus do not readily reveal their pathogenic armament outside of infected tissues
    1. regulate gene expression, typically by occluding or exposing regulatory features, such as ribosome binding sites (RBSs) in the case of translation or intrinsic terminator hairpins in the case of transcription
    1. In culture conditions that enhance cell-to-cell contact, conjugation rates approach 100% with the cis-acting plasmid

      high efficiency

    1. We report the construction of a family of vectors that contain a reengineered lacIq-lac promoter-operator complex in which cloned genes are strongly repressed in the absence of inducer.
  6. Aug 2019
  7. Jul 2019
  8. May 2019
  9. Mar 2019
    1. We have previously shown that antinociceptive effects of morphine are enhanced in histamine H1 receptor gene knockout mice.

      H1 antihistamines enhance the opioid high in humans. Hospitals sometimes administer antihistamines in combination with opioids. It's not hard to find people online who are using this combination recreationally.

  10. Jan 2019
  11. Nov 2018
  12. Oct 2018
  13. Sep 2018
  14. Jul 2018
    1. repeat expansion at IIL1 leads to increased accumulation of 24-nt siRNAs in a temperature-dependent manner that correlates with the iil phenotype. We show that DCL3 and other components of the RNA-dependent DNA methylation (RdDM) pathway are essential for this siRNA-directed epigenetic gene silencing
  15. Nov 2017
  16. Sep 2017
  17. Jul 2017
  18. Feb 2017
  19. Oct 2016
    1. blast

      BLAST finds regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance.

  20. Dec 2014
    1. This can not occur in the natural world.

      Genes from unrelated species may be incorporated in the wild by the process known as horizontal gene transfer.

      For example, approximately 8% of the human genome originated in viruses.

      Up to a quarter of the cow genome apparently originated in snakes, and was probably spread by ticks around the animal kingdom.