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    1. My own assessment is that the book, which reads like a thoroughly researched legal brief (more than 100 pages are devoted to notes, references and a very detailed index), makes the best possible case for the highly dubious proposition that the ideas of information theory influenced the substance, rather than merely the rhetoric, of research in molecular biology in the 1950s and 1960s.

      Information theorist Solomon Golomb, who directly participated in the applications of information theory to early genetics, doesn't feel that it influenced the substance of molecular biology in the 1950s and 1960s though it may have influenced the rhetoric.

    1. DelbruckJs Model Discussed and Tested

      n.b. Delbrück was Jim Watson’s postdoc advisor at Caltech

      see also:<br /> Golomb, Solomon W. Construction and Properties of Comma-Free Codes. With L. R. Welch and Max Delbrück, København, 1958. Biologiske Meddelelser Udg. Af Det Kongelige Danske Videnskabernes Selskab 23.

      Golomb, S. W., et al. “Comma-Free Codes.” Canadian Journal of Mathematics, vol. 10, Jan. 1958, pp. 202–09. Cambridge University Press, https://doi.org/10.4153/CJM-1958-023-9.

  2. May 2025
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    1. four 00:08:25 major common misunderstandings that have infected our understanding of what it is to be a living system

      for - molecular biology - paradigm shift - living system - 4 common misunderstandings - book - Understanding Living Systems - 4 common misunderstandings

      4 common misunderstandings of living systems - 1. The central dogma of molecular biology - one way causation - Genes (DNA) to - proteins to - organism - 2. The Weismann Barrier - 3. DNA as self-replicator - 4. Separation of Replicator (DNA) and Vehicle (Living cell) are completely separate

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    1. The several panels show what happens in each cycle. Each cycle consists of a denaturation step at a temperature higher than the melting temperature of the duplex DNA (e.g. 95 oC ), then an annealing step at a temperature below the melting temperature for the primer-template (e.g. 55 oC), followed by extension of the primer by DNA polymerase using dNTPs provided in the reaction. This is done at the temperature optimum for the DNA polymerase (e.g. 70 oC for a thermostable polymerase). Thermocylers are commercially available for carrying out many cycles quickly and reliably
      • Denature
      • Annealing primer
      • Synthesize new DNA with polymerase
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