Hamblin, J. (2021, December 9). Perspective | Omicron won’t keep us from ending the pandemic. We’re doing that ourselves. Washington Post. https://www.washingtonpost.com/outlook/2021/12/09/omicron-coronavirus-individual-threat/

Yangın algılama ve ihbar sistemleri nedir?

Skin Extract Preparation

Alarm substance was collected by making shallow lesions on the skin (7 to 10 on each side) using a sharp razor (Sharpoint, 22.5o stab), such that drawing of blood was minimal. Fish were then immersed in 2 ml of 20 mM Tris-Cl (pH 8.0) for 20-30 seconds. The process was repeated for 7 fish and extract collected into the same volume. The 2 ml crude extract was then centrifuged at 13.2k rpm and filtered. All steps were performed at 4 ̊C. 1 unit of extract was defined as the minimal amount of skin extract that must be delivered into the test tank to elicit freezing. A typical extraction yielded 20 units.

Sloughing was performed by mechanical shaking, either by inverting a 2 ml eppendorf tube with a euthanized fish without lesions or cuts in 2 ml of 20 mM Tris-Cl (pH 8.0), rapidly many times or on an orbital shaker for 1’.

For estimation of chondroitin concentration extraction was performed in 100 mM CH3COONH4 as described.

Korman, Maria, Vadim Tkachev, Cátia Reis, Yoko Komada, Shingo Kitamura, Denis Gubin, Vinod Kumar, and Till Roenneberg. ‘COVID-19-Mandated Social Restrictions Unveil the Impact of Social Time Pressure on Sleep and Body Clock’. Scientific Reports 10, no. 1 (17 December 2020): 22225. https://doi.org/10.1038/s41598-020-79299-7.

Jakie "alerty"? Alarmy. Angielski wyraz "alert" to po polsku "alarm". Czemu z uporem maniaka redakcja TNV24 forsuje słowo "alert"?