3 Matching Annotations
  1. Apr 2021
  2. Dec 2020
    1. Strive for a Tm between 50 and 65°C. One way to calculate Tm values is by using the nearest-neighbor method. Use the Tm calculator at http://www.basic.northwestern.edu/biotools/oligocalc.html, with values of 50 mM for salt concentration and 300 nM for oligonucleotide concentration
  3. Jun 2020
    1. Figure 6.1.46.1.4\PageIndex{4}: Temperature versus heat. The system is constructed so that no vapor evaporates while ice warms to become liquid water, and so that, when vaporization occurs, the vapor remains in the system. The long stretches of constant temperatures at 0oC0oC0^oC and 100oC100oC100^oC reflect the large amounts of heat needed to cause melting and vaporization, respectively.

      Figure 6.1.4 : Temperature versus heat. The system is constructed so that no vapor evaporates while ice warms to become liquid water, and so that, when vaporization occurs, the vapor remains in the system. The long stretches of constant temperatures at 0oC and 100oC reflect the large amounts of heat needed to cause melting and vaporization, respectively.