30 Matching Annotations
  1. Jan 2024
    1. a kanamycin resistance gene known to be expressed in both Gram-negative and Gram-positive bacteria

      aphA-3 gene from Campylobacter coli. Ref 21, 25 ~ quoted

      A 1427-bp DNA fragment containing the kanamycin resistance gene, aphA-3, of plasmid pIP1433 from Campylobacter coli was inserted into a shuttle vector. Full expression of aphA-3 was obtained in Bacillus subtilis and in Escherichia coli.

  2. Jan 2023
    1. what gives them their value is the role they play in a much larger system that's what we mean by dependence on conceptual imputation that's what we mean by broad 01:00:44 supervision

      !- definition: broad supervalence

    2. o the chariot doesn't just depend upon 00:56:29 the narrow base of parts it depends upon our conceptual imputation which is an essential idea for child security that what there is in the world depends not only on things 00:56:42 outside of us but also on how we think about things i call that broad super veniance if the chariot doesn't just depend on its parts it depends on the whole scheme in which chariots figure as 00:56:57 objects

      !- definition : broad superveniance - dependency on the entire associative network of ideas with which chariots are conceptually embedded

  3. Jun 2022
    1. Jacqueline Broad (Monash University)

      Online

      Short Bio

      Jacqueline Broad is an Associate Professor of Philosophy in the School of Philosophical, Historical, and International Studies at Monash University, Melbourne, Australia. She is also a Fellow of the Australian Academy of the Humanities.

      Her area of research expertise is early modern women’s philosophy. She writes on early modern theories of virtue, the ethical and religious foundations of women’s rights, historical conceptions of the self, and connections between feminism and Cartesianism in the seventeenth century.

      She has recently become Series Editor for Cambridge University Press’s new Elements series on Women in the History of Philosophy.

      Select bibliography

      • Jacqueline Broad, ‘Undoing Bayle’s Scepticism: Astell’s Marginalia as Disarmament’, in Marginal Notes: Social Reading and the Literal Margins, edited by Patrick Spedding and Paul Tankard (New York: Palgrave Macmillan, 2021), pp. 61–84.
  4. Jan 2022
    1. Maybe once the core onError lifecycle is implemented (if maintainers decide to go that way) everyone will discover you're right and an implementation will be built in. I think that's probably what's going to happen. But until real life has proved it, it's usually best to go for the smallest most broadly applicable solution. I can definitely imagine <svelte:error> eventually being a thing, but it's a pretty dramatic change compared to an added importable function.
  5. Feb 2021
    1. As Miller explained, it is central to the radical Left’s foundation to destroy Americans’ faith in ourselves, our past, and our present. Because this destruction is the prerequisite for changing America into a dramatically different country, which is less free, less safe, and less successful.

      Why are Republicans always trying to use the "radical Left" as the boogeyman and painting the entirety of the Democratic party with it? Gingrich knows full well that there are marginal voices on the far left just as there are on the far right. Why are Republicans always painting with such a broad brush and not supporting their arguments with actual facts?

      I'm curious just which specific things Gingerich thinks they're doing that will make us "less free" or "less safe"? For comparison, maybe we should look at decades of "trickle down economics" and see if those work? I suspect Gingrich and Miller would say yes, but mountains of evidence indicate we're not measurably better off. (See also: http://eprints.lse.ac.uk/107919/1/Hope_economic_consequences_of_major_tax_cuts_published.pdf)

  6. Jan 2021
    1. RSF1010 is maintained at a copy-number of about 10-12 per chromosome in E coli, P. aeruginosa and S. enterica sv.Typhimurium (Frey and Bagdasarian, 1989)
    1. This work provides CasTn as a new method for host-independent, programmable, targeted DNA insertions to expand the genomic engineering toolbox.
  7. Dec 2020
    1. The pBHR1 plasmid is a derivative of the small (2.6-kb), mobilizable broad-host-range plasmid pBBR1, which was isolated from the gram-negative bacterium Bordetella bronchiseptica

      pBBR1

  8. Nov 2020
    1. Among the fully sequenced BHR plasmids, most are classified as the well-known incompatibility groups, such as IncP-1 (41 plasmids), IncW (5 plasmids), and IncU (4 plasmids), based on the backbone genes
  9. Oct 2020
    1. I don't think we want the cookbook to become a collection of tutorials regarding how to use certain libraries and tools with svelte. A set of more generic recipes or deep dives into topics would probably serve people better and have broader application.
  10. Jul 2020
    1. A growing number of platforms, vendors, and partners support the AMP Project by providing custom components or offering integration with AMP pages within their platforms.

      I guess AMP is actually open-source software, but it still feels like it's something non-standard. I guess it's just an alternative open standard to the "main" web open standards.

    1. If you have worked with emails before, the idea of placing a script into an email may set off alarm bells in your head! Rest assured, email providers who support AMP emails enforce fierce security checks that only allow vetted AMP scripts to run in their clients. This enables dynamic and interactive features to run directly in the recipients mailboxes with no security vulnerabilities! Read more about the required markup for AMP Emails here.
  11. May 2020
    1. Not building a static site? No worries there, React Static works as an SPA too, even if there is only a single index.html file.
    1. the expanding selection of CMS options for site generators remove the need to maintain a separate stack for content and marketing.
    1. In mathematical contexts, duality has numerous meanings[1] although it is "a very pervasive and important concept in (modern) mathematics"[2] and "an important general theme that has manifestations in almost every area of mathematics".[3]
    1. This scope effectively covers almost all companies and, therefore, means that the GDPR can apply to you whether your organization is based in the EU or not.
    1. This scope effectively covers almost all companies and, therefore, means that the GDPR can apply to you whether your organization is based in the EU or not. As a matter of fact, this PwC survey showed that the GDPR is a top data protection priority for up to 92 percent of U.S. companies surveyed.
  12. Apr 2020
    1. It will allow anyone to annotate anything anywhere, be it a web page, an ebook, a video, an image, an audio stream, or data in raw or visualized form
  13. Feb 2020
    1. The host range for three of the plasmids (pBF1, pB7, and pB9) was further investigated by the GFP method described above

      Does the host range of transfer go wider than the population where GFP is observed?

      This is my idea of distinctive overlapping categories of host range as venn diagrams.

  14. Jan 2020
    1. Plasmid pBBR1 thus appears to be a new member of this group, even though it resides in gram-negative bacteria and does not replicate via a rolling-circle mechanism
  15. Oct 2019
    1. RP4 has been shown to exhibit a very broad host range and has been demonstrated to efficiently transfer in activated sludge (22, 40), chicken manure (24), and the rhizospheres of various plants (38, 39).
  16. Jul 2016