10,000 Matching Annotations
  1. Jul 2025
  2. elifesciences.org elifesciences.org
    PD-linked LRRK2 G2019S mutation impairs astrocyte morphology and synapse maintenance via ERM hyperphosphorylation
    18
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/107556
  3. elifesciences.org elifesciences.org
    Astrocytic modulation of population encoding in mouse visual cortex via GABA transporter 3 revealed by multiplexed CRISPR/Cas9 gene editing
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/107298
  4. elifesciences.org elifesciences.org
    Inducible, virus-free direct lineage reprogramming enhances scalable generation of human inner ear hair cell-like cells
    3
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106550
  5. elifesciences.org elifesciences.org
    Human formin FHOD3-mediated actin elongation is required for sarcomere integrity in cardiomyocytes
    8
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/104048
  6. elifesciences.org elifesciences.org
    Ribozyme-catalysed RNA synthesis using triplet building blocks
    8
    1. Kevingomezgg 15 Jul 2025
      t5+1 could assemble these into αβ+ and γδε+ fragments, guided only by partially overlapping ‘−’ strands (Figure 6b, Figure 6—figure supplement 1). Through non-covalent association (Vaish et al., 2003; Mutschler et al., 2015), the ribozyme-synthesised αβ+ and γδε+ fragments spontaneously reconstituted a new catalytically active triplet polymerase ribozyme (with in vitro transcribed type 1 RNA)

      The ribozyme can build copies of parts of itself and put them together like puzzle pieces to regenerate function. That’s basically modular self-replication, which I thought only synthetic biology was capable of.

    2. Kevingomezgg 15 Jul 2025
      RNA catalysis is dependent upon RNA folding, and this yields structures that can block replication of such RNAs. To address this apparent paradox, we have re-examined the building blocks used for RNA replication.

      This blew my mind, RNA has to fold into specific shapes to work like a ribozyme, but those same shapes make it harder to copy. It’s like building a key that locks itself.

    3. abrasiliano 12 Jul 2025
      Although still inefficient, the fact that the nascent activity of the 0core ribozyme could already copy templates that had confounded an established RPR encouraged us to continue to seek improved triplet polymerase ribozymes to leverage this substrate behaviour.

      This early success gave the researchers a good reason to keep pushing forward, since it hinted that triplet-based RNA replication might help overcome a key obstacle in the field.

    4. abrasiliano 12 Jul 2025
      he strong inhibitory role of this central feature of RNA leads to an antagonism between the degree to which an RNA sequence is able to fold into a defined three-dimensional structure to encode function (such as catalysis) and the ease with which it can be replicated (Boza et al., 2014).

      This "structure vs. replication" tradeoff is a bigger deal because it may have made it harder for life to get started using RNA as both the genetic material and the machinery for replication.

    5. abrasiliano 12 Jul 2025
      Yet, although ribozymes can be made to copy straight RNA templates this way, folded RNA templates – including the replicase ribozyme itself – impede copying. In this apparent paradox, a ribozyme needs to fold to copy RNA, but when folded, is itself copied poorly. Here, Attwater et al. wondered if choosing different building blocks might overcome this contradiction.

      I think this presents a contradiction, that the structure hat enables function also hinders duplication. However, the sentence introduces a key barrier that the research seeks to address.

    6. Victoria23 07 Jul 2025
      RNA oligonucleotides

      This is short, synthetic strands of RNA, typically ranging from 2 to 50 nucleotides in length, used in various molecular biology applications

    7. Victoria23 07 Jul 2025
      template secondary structures.

      This is the folded conformations that a DNA or RNA template can adopt due to intramolecular base pairing, forming structures like hairpins or stem-loops, It can interrupt or intervene with DNA replication, transcription, translation and etc.

    8. Victoria23 07 Jul 2025
      no replicase ribozyme has been observed in existing life forms;

      Most likely because they have been replaced by more efficient protein enzymes due to evolution.

    Visit annotations in context

    Annotators

    URL

    elifesciences.org/articles/35255
  7. elifesciences.org elifesciences.org
    The BRAIN Initiative data-sharing ecosystem: Characteristics, challenges, benefits, and opportunities
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/94000
  8. elifesciences.org elifesciences.org
    Endogenous oscillatory rhythms and interactive contingencies jointly influence infant attention during early infant-caregiver interaction
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/88775
  9. elifesciences.org elifesciences.org
    Transcranial focused ultrasound to human rIFG improves response inhibition through modulation of the P300 onset latency
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/86190
  10. elifesciences.org elifesciences.org
    Interplay between acetylation and ubiquitination of imitation switch chromatin remodeler Isw1 confers multidrug resistance in Cryptococcus neoformans
    2
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/85728
  11. elifesciences.org elifesciences.org
    Leveraging place field repetition to understand positional versus nonpositional inputs to hippocampal field CA1
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/85599
  12. elifesciences.org elifesciences.org
    Claudin5 protects the peripheral endothelial barrier in an organ and vessel-type-specific manner
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/78517
  13. elifesciences.org elifesciences.org
    Structural mechanism of strand exchange by the RAD51 filament
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/107114v1
  14. elifesciences.org elifesciences.org
    Gut Microbe-Derived Trimethylamine Shapes Circadian Rhythms Through the Host Receptor TAAR5
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/107037v1
  15. elifesciences.org elifesciences.org
    Membrane curvature regulates Ups1 dependent phosphatidic acid transfer across lipid bilayers
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106979v1
  16. elifesciences.org elifesciences.org
    Sparse innervation and local heterogeneity in the vibrissal corticostriatal projection
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106621v1
  17. elifesciences.org elifesciences.org
    Calibration and validation strategy for electromechanical cardiac digital twins
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106555v1
  18. elifesciences.org elifesciences.org
    CHROMAS: A Computational Pipeline to Track Chromatophores and Analyze their Dynamics
    9
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106509v1
  19. elifesciences.org elifesciences.org
    Coenzyme A governs proinflammatory macrophage metabolism
    2
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106473
  20. elifesciences.org elifesciences.org
    CRISPR-edited DPSCs constitutively expressing BDNF enhance dentin regeneration in injured teeth
    9
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/105153
  21. elifesciences.org elifesciences.org
    A temporally restricted function of the dopamine receptor Dop1R2 during memory formation
    12
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/99368
  22. elifesciences.org elifesciences.org
    Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/107534
  23. elifesciences.org elifesciences.org
    The human cytomegalovirus-encoded pUS28 antagonizes CD4+ T cell recognition by targeting CIITA
    6
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/96414
  24. elifesciences.org elifesciences.org
    A geometric shape regularity effect in the human brain
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106464v1
  25. elifesciences.org elifesciences.org
    Making plant tissue accessible for cryo-electron tomography
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106455v1
  26. elifesciences.org elifesciences.org
    Cross-species insemination reveals mouse sperm ability to enter and cross the fish micropyle
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106303v1
  27. elifesciences.org elifesciences.org
    Multifaceted role of galanin in brain excitability
    3
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/98634
  28. elifesciences.org elifesciences.org
    Highly sensitive in vivo detection of dynamic changes in enkephalins following acute stress in mice
    13
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/91609
  29. elifesciences.org elifesciences.org
    Competence for transcellular infection in the root cortex involves a post-replicative, cell-cycle exit decision in Medicago truncatula
    10
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/88588
  30. elifesciences.org elifesciences.org
    The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence
    42
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/101984
  31. elifesciences.org elifesciences.org
    The yellow gene influences Drosophila male mating success through sex comb melanization
    7
    1. abrasiliano 07 Jul 2025
      These observations suggest that the pleiotropic effects of yellow on male mating success might result from effects of yellow in the adult CNS, particularly in fru-expressing neurons.

      This sentence really shows how complex and interconnected gene function can be. It's not just that the yellow gene affects pigmentation, it might also influence behavior through its role in specific brain cells.

    2. abrasiliano 07 Jul 2025
      It seemed worthwhile therefore to examine more closely one example of a gene mutation affecting behavior and to ask two questions, (1) how does it bring about its effect? [and], (2) what part might it play in evolution?”

      Even back in 1956, Bastock was asking questions that are still relevant today, not just about how a gene affects behavior, but also what that means in terms of evolution.

    3. abrasiliano 07 Jul 2025
      The study reveals the importance of scientists considering that genes that affect behavior may do so by changing anatomy rather than by altering the brain

      This is a reminder to scientists (and us) that behavior isn’t just about brain chemistry; sometimes it’s about how the body is built.

    4. Kevingomezgg 02 Jul 2025
      Yellow protein is expressed in sex combs (Hinaux et al., 2018, Figure 3G,H), where it is presumably required for synthesis of black dopamine melanin in the sex comb ‘teeth’.

      Melanization = adding dark pigment (melanin) to certain body parts. In this case, melanization makes the sex combs stronger and more rigid, helping males grab females. Without yellow, the sex combs are lighter and weaker.

    5. Kevingomezgg 02 Jul 2025
      t expression of yellow in fru-expressing cells is neither necessary nor sufficient for yellow’s effect on male mating success.

      Yellow doesn’t need to be in brain cells to affect mating; the problem is somewhere else.

    6. Kevingomezgg 02 Jul 2025
      The yellow males lack melanin pigments in their sex combs, which changes their structure.

      Key shift from neural to anatomical explanation, behavioral defect actually caused by structural changes in sex combs, not brain chemistry.

    7. dmays 02 Jul 2025
      However, we found that suppressing yellow expression in the larval CNS, dopaminergic neurons, or serotonergic neurons (Figure 2—figure supplement 3, FET, P values ranging from 0.45 to 1), or in all neurons (Figure 2E, FET, p=1 in all cases) or all glia (Figure 2F, FET, p=1), had no significant effect on male mating success.

      This result was surprising because it shows that turning off yellow in key parts of the nervous system—including neurons involved in mood and movement—does not affect male mating behavior. This suggests that yellow doesn’t act in the brain or nerves to influence mating, challenging the original hypothesis.

    Visit annotations in context

    Annotators

    URL

    elifesciences.org/articles/49388
  32. elifesciences.org elifesciences.org
    Integrated transcriptomic analysis of human induced pluripotent stem cell-derived osteogenic differentiation reveals a regulatory role of KLF16
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106265v1
  33. elifesciences.org elifesciences.org
    Evidence for systematic - yet task- and motor-contingent - rhythmicity of auditory perceptual judgements
    2
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/105734v1
  34. elifesciences.org elifesciences.org
    Structural mechanisms of PIP2 activation and SEA0400 inhibition in human cardiac sodium-calcium exchanger NCX1
    3
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/105396v2
  35. elifesciences.org elifesciences.org
    mTOR inhibition in Q175 Huntington’s disease model mice facilitates neuronal autophagy and mutant huntingtin clearance
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/104979v2
  36. elifesciences.org elifesciences.org
    Engineering cardiolipin binding to an artificial membrane protein reveals determinants for lipid-mediated stabilization
    2
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/104237
  37. elifesciences.org elifesciences.org
    Birds migrate longitudinally in response to the resultant Asian monsoons of the Qinghai-Tibet Plateau uplift
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/103971v2
  38. elifesciences.org elifesciences.org
    Exploiting functional regions in the viral RNA genome as druggable entities
    2
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/103923v2
  39. elifesciences.org elifesciences.org
    Expanding Automated Multiconformer Ligand Modeling to Macrocycles and Fragments
    1
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/103797v2
  40. elifesciences.org elifesciences.org
    Progressive overfilling of readily releasable pool underlies short-term facilitation at recurrent excitatory synapses in layer 2/3 of the rat prefrontal cortex
    3
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/102923v2
  41. elifesciences.org elifesciences.org
    SIRT2 protects against Japanese encephalitis virus infection in mice
    4
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/106778
  42. elifesciences.org elifesciences.org
    Planar cell polarity coordination in a cnidarian embryo provides clues to animal body axis evolution
    6
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/articles/104508
  43. elifesciences.org elifesciences.org
    An increase in reactive oxygen species underlies neonatal cerebellum repair
    2
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/102515v2
  44. elifesciences.org elifesciences.org
    Identification and characterization of early human photoreceptor states and cell-state-specific retinoblastoma-related features
    5
    Visit annotations in context

    Tags

    Annotators

    URL

    elifesciences.org/reviewed-preprints/101918v2