19 Matching Annotations
  1. Apr 2021
    1. Sweetshrub grows best in average to rich, well-drained soil in anywhere from full sun to deep shade. It prefers some shade in hot summer afternoons and it will grow lankier and less dense in shade than in sun. It is tolerant of a wide range of soil textures and pH but prefers rich loams.  Plant it 3 to 5 feet from other shrubs to give it adequate room to grow.  It blooms in early spring before leaves emerge, with the leaves, and sporadically thereafter.  

      Very tolerant of shade or sun and most soils.

  2. Jan 2021
    1. RSF1010 is maintained at a copy-number of about 10-12 per chromosome in E coli, P. aeruginosa and S. enterica sv.Typhimurium (Frey and Bagdasarian, 1989)
    1. This work provides CasTn as a new method for host-independent, programmable, targeted DNA insertions to expand the genomic engineering toolbox.
  3. Dec 2020
    1. The pBHR1 plasmid is a derivative of the small (2.6-kb), mobilizable broad-host-range plasmid pBBR1, which was isolated from the gram-negative bacterium Bordetella bronchiseptica

      pBBR1

  4. Nov 2020
    1. Among the fully sequenced BHR plasmids, most are classified as the well-known incompatibility groups, such as IncP-1 (41 plasmids), IncW (5 plasmids), and IncU (4 plasmids), based on the backbone genes
  5. Oct 2020
  6. Aug 2020
  7. Jul 2020
    1. Why don't you allow a range without end, like (1..)? There are two advantages. First, we can write ary[1..] instead of ary[1..-1]. The -1 is one of the most I dislike in Ruby. It is very magical, ugly, redundant, and disappointing. I envy Python's ary[1:]. I know that ary.drop(1) is slightly fast, but too long for such a common operation, IMO. Second, we can write (1..).each {|n| ... }.
  8. Jun 2020
  9. May 2020
  10. Feb 2020
    1. The host range for three of the plasmids (pBF1, pB7, and pB9) was further investigated by the GFP method described above

      Does the host range of transfer go wider than the population where GFP is observed?

      This is my idea of distinctive overlapping categories of host range as venn diagrams.

  11. Jan 2020
    1. Plasmid pBBR1 thus appears to be a new member of this group, even though it resides in gram-negative bacteria and does not replicate via a rolling-circle mechanism
  12. Dec 2019
  13. Oct 2019
    1. RP4 has been shown to exhibit a very broad host range and has been demonstrated to efficiently transfer in activated sludge (22, 40), chicken manure (24), and the rhizospheres of various plants (38, 39).
  14. Jan 2019
  15. Oct 2016