10,000 Matching Annotations
  1. Sep 2021
  2. elifesciences.org elifesciences.org
    Single-molecule observation of ATP-independent SSB displacement by RecO in Deinococcus radiodurans
    4
    1. aksmit22 22 Sep 2021
      The first binding between drRecO and ssDNA occurs in the drSSB-free space of ssDNA and generates a heterotrimer of the drSSB-ssDNA-drRecO complex as an intermediate state. The second binding between drRecO and ssDNA induces the dissociation of drSSB from ssDNA by facilitating a conformational change in ssDNA.

      So ultimately from these findings there are two steps involved in a conformational change in the ssDNA and can avoid using ATP?

    2. aksmit22 22 Sep 2021
      molecular mechanism of D. radiodurans (DR) RecO (drRecO) in SSB displacement from ssDNA at the single-molecule level. DR is the toughest bacterium known, with outstanding resistance to ionizing radiation and DNA damage-inducing reagents (Blasius et al., 2008; Cox and Battista, 2005; Slade and Radman, 2011), which are the main causes of DSBs, a form of fatal biological damage at the genomic level

      I wonder if a section of the genome that helps encode for this mechanism and trait for resistance to ionizing radiation can be inserted in other organisms or be used to prevent DNA damage from radiation

    3. wcmarx 21 Sep 2021
      In summary, our results demonstrate that drRecO binds to ssDNA sequentially using its two binding sites, which gives drRecO the ability to displace drSSB from ssDNA even though the binding affinity of drRecO for ssDNA is two orders of magnitude smaller than that of drSSB.

      It is amazing that cells are capable of performing actions like this and the researchers were able to show this through their results. I would usually assume that a molecule would need a stronger binding affinity to displace another bound protein, so it was interesting to learn how DrRecO can accomplish this with a much lower binding affinity.

    4. tkduncan 21 Sep 2021
      RecO is believed to play a key role in this step because RecO is able to interact with SSB, ssDNA, and dsDNA simultaneously

      So (if I'm interpreting this correctly) RecO is thought to be involved in helping to recruit the rest of the RecFOR machinery to the site of the break because it can interact with SSB, ssDNA, and dsDNA even though SSB binds strongly to ssDNA, right?

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    elifesciences.org/articles/50945
  3. Aug 2021
  4. elifesciences.org elifesciences.org
    Natural variation in the consequences of gene overexpression and its implications for evolutionary trajectories
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    elifesciences.org/articles/70564
  5. elifesciences.org elifesciences.org
    The GNU subunit of PNG kinase, the developmental regulator of mRNA translation, binds BIC-C to localize to RNP granules
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    elifesciences.org/articles/67294
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    An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites
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    elifesciences.org/articles/68268
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    HOXA9 promotes MYC-mediated leukemogenesis by maintaining gene expression for multiple anti-apoptotic pathways
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    elifesciences.org/articles/64148
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    miR-1 coordinately regulates lysosomal v-ATPase and biogenesis to impact proteotoxicity and muscle function during aging
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    elifesciences.org/articles/66768
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    VEGF/VEGFR2 signaling regulates hippocampal axon branching during development
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    elifesciences.org/articles/49818
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    Soluble PD-L1 generated by endogenous retroelement exaptation is a receptor antagonist
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    elifesciences.org/articles/50256
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    A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in brain neurons
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    elifesciences.org/articles/48750
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    Taste bud formation depends on taste nerves
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    elifesciences.org/articles/49226
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    An open cortico-basal ganglia loop allows limbic control over motor output via the nigrothalamic pathway
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    elifesciences.org/articles/49995
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    CCN1 interlinks integrin and Hippo pathway to autoregulate tip cell activity
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    elifesciences.org/articles/46012
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    Mutationally-activated PI3’-kinase-α promotes de-differentiation of lung tumors initiated by the BRAFV600E oncoprotein kinase
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    elifesciences.org/articles/43668
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    Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 crosstalk
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    elifesciences.org/articles/47646
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    RbFox1 up-regulation impairs BDNF-dependent hippocampal LTP by dysregulating TrkB isoform expression levels
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    elifesciences.org/articles/49673
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    Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization
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    elifesciences.org/articles/49677
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    Epimutations are associated with CHROMOMETHYLASE 3-induced de novo DNA methylation
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    elifesciences.org/articles/47891
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    The antimicrobial peptide defensin cooperates with tumour necrosis factor to drive tumour cell death in Drosophila
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    elifesciences.org/articles/45061
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    Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
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    elifesciences.org/articles/18825
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    27 T ultra-high static magnetic field changes orientation and morphology of mitotic spindles in human cells
    8
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    elifesciences.org/articles/22911
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    A Cryptochrome 2 mutation yields advanced sleep phase in humans
    7
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    elifesciences.org/articles/16695
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    The retromer complex safeguards against neural progenitor-derived tumorigenesis by regulating Notch receptor trafficking
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    elifesciences.org/articles/38181
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    GIPC proteins negatively modulate Plexind1 signaling during vascular development
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    elifesciences.org/articles/30454
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    Untitled document
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    elifesciences.org/articles/30224
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    elifesciences.org/articles/30657
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    elifesciences.org/articles/20898
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    Replication Study: Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs
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    elifesciences.org/articles/17584
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    elifesciences.org/articles/19594
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    elifesciences.org/articles/21470
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    elifesciences.org/articles/20059
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    elifesciences.org/articles/42434
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    elifesciences.org/articles/44912