Given the lenght of what Bitcoin it's trying to accomplish (overtake the financial system), the electricity consumption is not unacceptable I would say. In a 2021 study it came out that Bitcoin consumption of electricity is still less than half of the current traditional financial system.

SciScore for 10.1101/2022.02.23.22271388: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

No key resources detected.

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

In particular, because the question on Long Covid was not introduced until 3 February 2021, shortly after mass COVID-19 vaccination started in the UK on 8 December 2020, one key limitation is that it was not possible to match double-vaccinated and unvaccinated participants on calendar time of infection (Supplementary Figure 1). Differences in the likelihood of developing Long Covid symptoms between exposure groups may therefore partly reflect changes in the dominant COVID-19 variant or other period effects. Our key exposure was double vaccination, despite third and booster doses now being available, and the study period was before the Omicron variant became widespread. We were not able to investigate participants who were single-vaccinated when infected because nearly all of these received their second dose within the 12-week follow-up period, confounding any relationship between one dose at infection and Long Covid symptoms. In conclusion, SARS-CoV-2 infection after double vaccination is associated with a reduced risk of developing Long Covid symptoms at 12 weeks compared with infection before vaccination, emphasising the need for public health initiatives to increase population-level vaccine uptake. Studies with longer follow-up are needed to assess the impact of booster doses and the Omicron variant and to evaluate symptom trajectories beyond a single 12-week follow-up visit, particularly given the relapsing nature of Long Covid [14]. Further research into possible biologi...

Results from TrialIdentifier: We found the following clinical trial numbers in your paper:<br><table><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">Identifier</td><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">Status</td><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">Title</td></tr><tr><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">ISRCTN21086382</td><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">NA</td><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">NA</td></tr></table>

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

Results from rtransparent:

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

Results from scite Reference Check: We found no unreliable references.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

Good as a source for credibility. Many people including historians and christians know of Thomas Paine and his work.

1.5 million people are half the content. Reminds me of a similar statistic about Twitter and how "what twitter thinks" only represents a subset, and probably a selectively chosen subset, of the population

Dependence on slave labor; the sacrifice of the Africans is necessary for the market economy to thrive and for Europe to have access to these precious ressources

note that the work of these slaves is called "agriculture", which compares them to farmers and makes it look as though their work was human and not dehumanizing

anti-matter to David Allen

Never getting Things done

Quite right too

Getting Things Done

assumes that you know what IT is that you need to do right here right now

But if you inclined to ask the question what is the IT that we really need to do

GTD is not for you at this stage

So how about a voluntary breakup? Something that the unfederated networks would never do unless trying to avoid being the target of litigation for anti-competitive practices, but where is the pressure to refrain from doing this for mastodon.social or anywhere else in the fediverse?

We have had and still are dealing with Covid-19 which has infected and killed many people, but not has done near anything close to this damage. Imagine how big of a deal dealing with the Black Death must've been for Europeans.

Although Canada's population would grow massively in the decades to come, it still had a ways to go in terms of numbers coming into the 20th century, with a population of around 5-6 million people.

Poor working opportunities for refugees

Similar to D-CENT which I actually participated in!

SciScore for 10.1101/2021.12.25.474152: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

Results from scite Reference Check: We found no unreliable references.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

Economics of insight timer. Key thing has been investment that allows them to lose a lot of money early on and stay very true to the mission.

GW mentions "various elemnts of the modern human condition" converged. What elements would they be?

According to all known laws of aviation,

there is no way a bee should be able to fly.

Its wings are too small to get its fat little body off the ground.

The bee, of course, flies anyway

because bees don't care what humans think is impossible.

Yellow, black. Yellow, black. Yellow, black. Yellow, black.

Ooh, black and yellow! Let's shake it up a little.

Barry! Breakfast is ready!

Ooming!

Hang on a second.

Hello?

• Barry?
• Adam?

• Oan you believe this is happening?
• I can't. I'll pick you up.

Looking sharp.

Use the stairs. Your father paid good money for those.

Sorry. I'm excited.

Here's the graduate. We're very proud of you, son.

A perfect report card, all B's.

Very proud.

Ma! I got a thing going here.

• You got lint on your fuzz.
• Ow! That's me!

• Wave to us! We'll be in row 118,000.
• Bye!

Barry, I told you, stop flying in the house!

• Hey, Adam.
• Hey, Barry.

• Is that fuzz gel?
• A little. Special day, graduation.

Never thought I'd make it.

Three days grade school, three days high school.

Those were awkward.

Three days college. I'm glad I took a day and hitchhiked around the hive.

You did come back different.

• Hi, Barry.
• Artie, growing a mustache? Looks good.

• Hear about Frankie?
• Yeah.

• You going to the funeral?
• No, I'm not going.

Everybody knows, sting someone, you die.

Don't waste it on a squirrel. Such a hothead.

I guess he could have just gotten out of the way.

I love this incorporating an amusement park into our day.

That's why we don't need vacations.

Boy, quite a bit of pomp... under the circumstances.

• Well, Adam, today we are men.
• We are!

• Bee-men.
• Amen!

Hallelujah!

Students, faculty, distinguished bees,

please welcome Dean Buzzwell.

Welcome, New Hive Oity graduating class of...

...9:15.

That concludes our ceremonies.

And begins your career at Honex Industries!

Will we pick ourjob today?

I heard it's just orientation.

Heads up! Here we go.

Keep your hands and antennas inside the tram at all times.

• Wonder what it'll be like?
• A little scary.

Welcome to Honex, a division of Honesco

and a part of the Hexagon Group.

This is it!

Wow.

Wow.

We know that you, as a bee, have worked your whole life

to get to the point where you can work for your whole life.

Honey begins when our valiant Pollen Jocks bring the nectar to the hive.

Our top-secret formula

is automatically color-corrected, scent-adjusted and bubble-contoured

into this soothing sweet syrup

with its distinctive golden glow you know as...

Honey!

• That girl was hot.
• She's my cousin!

• She is?
• Yes, we're all cousins.

• Right. You're right.
• At Honex, we constantly strive

to improve every aspect of bee existence.

These bees are stress-testing a new helmet technology.

• What do you think he makes?
• Not enough.

Here we have our latest advancement, the Krelman.

• What does that do?
• Oatches that little strand of honey

that hangs after you pour it. Saves us millions.

Oan anyone work on the Krelman?

Of course. Most bee jobs are small ones. But bees know

that every small job, if it's done well, means a lot.

But choose carefully

because you'll stay in the job you pick for the rest of your life.

The same job the rest of your life? I didn't know that.

What's the difference?

You'll be happy to know that bees, as a species, haven't had one day off

in 27 million years.

So you'll just work us to death?

We'll sure try.

Wow! That blew my mind!

"What's the difference?" How can you say that?

One job forever? That's an insane choice to have to make.

I'm relieved. Now we only have to make one decision in life.

But, Adam, how could they never have told us that?

Why would you question anything? We're bees.

We're the most perfectly functioning society on Earth.

You ever think maybe things work a little too well here?

Like what? Give me one example.

I don't know. But you know what I'm talking about.

Please clear the gate. Royal Nectar Force on approach.

Wait a second. Oheck it out.

• Hey, those are Pollen Jocks!
• Wow.

I've never seen them this close.

They know what it's like outside the hive.

Yeah, but some don't come back.

• Hey, Jocks!
• Hi, Jocks!

You guys did great!

You're monsters! You're sky freaks! I love it! I love it!

• I wonder where they were.
• I don't know.

Their day's not planned.

Outside the hive, flying who knows where, doing who knows what.

You can'tjust decide to be a Pollen Jock. You have to be bred for that.

Right.

Look. That's more pollen than you and I will see in a lifetime.

It's just a status symbol. Bees make too much of it.

Perhaps. Unless you're wearing it and the ladies see you wearing it.

Those ladies? Aren't they our cousins too?

Distant. Distant.

Look at these two.

• Oouple of Hive Harrys.
• Let's have fun with them.

It must be dangerous being a Pollen Jock.

Yeah. Once a bear pinned me against a mushroom!

He had a paw on my throat, and with the other, he was slapping me!

• Oh, my!
• I never thought I'd knock him out.

What were you doing during this?

Trying to alert the authorities.

I can autograph that.

A little gusty out there today, wasn't it, comrades?

Yeah. Gusty.

We're hitting a sunflower patch six miles from here tomorrow.

• Six miles, huh?
• Barry!

A puddle jump for us, but maybe you're not up for it.

• Maybe I am.
• You are not!

We're going 0900 at J-Gate.

What do you think, buzzy-boy? Are you bee enough?

I might be. It all depends on what 0900 means.

Hey, Honex!

Dad, you surprised me.

You decide what you're interested in?

• Well, there's a lot of choices.
• But you only get one.

Do you ever get bored doing the same job every day?

Son, let me tell you about stirring.

You grab that stick, and you just move it around, and you stir it around.

You get yourself into a rhythm. It's a beautiful thing.

You know, Dad, the more I think about it,

maybe the honey field just isn't right for me.

You were thinking of what, making balloon animals?

That's a bad job for a guy with a stinger.

Janet, your son's not sure he wants to go into honey!

• Barry, you are so funny sometimes.
• I'm not trying to be funny.

You're not funny! You're going into honey. Our son, the stirrer!

• You're gonna be a stirrer?
• No one's listening to me!

Wait till you see the sticks I have.

I could say anything right now. I'm gonna get an ant tattoo!

Let's open some honey and celebrate!

Maybe I'll pierce my thorax. Shave my antennae.

Shack up with a grasshopper. Get a gold tooth and call everybody "dawg"!

I'm so proud.

• We're starting work today!
• Today's the day.

Oome on! All the good jobs will be gone.

Yeah, right.

Pollen counting, stunt bee, pouring, stirrer, front desk, hair removal...

• Is it still available?
• Hang on. Two left!

One of them's yours! Oongratulations! Step to the side.

• What'd you get?
• Picking crud out. Stellar!

Wow!

Oouple of newbies?

Yes, sir! Our first day! We are ready!

Make your choice.

• You want to go first?
• No, you go.

Oh, my. What's available?

Restroom attendant's open, not for the reason you think.

• Any chance of getting the Krelman?
• Sure, you're on.

I'm sorry, the Krelman just closed out.

Wax monkey's always open.

The Krelman opened up again.

What happened?

A bee died. Makes an opening. See? He's dead. Another dead one.

Deady. Deadified. Two more dead.

Dead from the neck up. Dead from the neck down. That's life!

Oh, this is so hard!

Heating, cooling, stunt bee, pourer, stirrer,

humming, inspector number seven, lint coordinator, stripe supervisor,

mite wrangler. Barry, what do you think I should... Barry?

Barry!

All right, we've got the sunflower patch in quadrant nine...

What happened to you? Where are you?

• I'm going out.
• Out? Out where?

• Out there.
• Oh, no!

I have to, before I go to work for the rest of my life.

You're gonna die! You're crazy! Hello?

Another call coming in.

If anyone's feeling brave, there's a Korean deli on 83rd

that gets their roses today.

Hey, guys.

• Look at that.
• Isn't that the kid we saw yesterday?

Hold it, son, flight deck's restricted.

It's OK, Lou. We're gonna take him up.

Really? Feeling lucky, are you?

Sign here, here. Just initial that.

• Thank you.
• OK.

You got a rain advisory today,

and as you all know, bees cannot fly in rain.

So be careful. As always, watch your brooms,

hockey sticks, dogs, birds, bears and bats.

Also, I got a couple of reports of root beer being poured on us.

Murphy's in a home because of it, babbling like a cicada!

• That's awful.
• And a reminder for you rookies,

bee law number one, absolutely no talking to humans!

All right, launch positions!

Buzz, buzz, buzz, buzz! Buzz, buzz, buzz, buzz! Buzz, buzz, buzz, buzz!

Black and yellow!

Hello!

You ready for this, hot shot?

Yeah. Yeah, bring it on.

Wind, check.

• Antennae, check.
• Nectar pack, check.

• Wings, check.
• Stinger, check.

Scared out of my shorts, check.

OK, ladies,

let's move it out!

Pound those petunias, you striped stem-suckers!

All of you, drain those flowers!

Wow! I'm out!

I can't believe I'm out!

So blue.

I feel so fast and free!

Box kite!

Wow!

Flowers!

This is Blue Leader. We have roses visual.

Bring it around 30 degrees and hold.

Roses!

30 degrees, roger. Bringing it around.

Stand to the side, kid. It's got a bit of a kick.

That is one nectar collector!

• Ever see pollination up close?
• No, sir.

I pick up some pollen here, sprinkle it over here. Maybe a dash over there,

a pinch on that one. See that? It's a little bit of magic.

That's amazing. Why do we do that?

That's pollen power. More pollen, more flowers, more nectar, more honey for us.

Oool.

I'm picking up a lot of bright yellow. Oould be daisies. Don't we need those?

Oopy that visual.

Wait. One of these flowers seems to be on the move.

Say again? You're reporting a moving flower?

Affirmative.

That was on the line!

This is the coolest. What is it?

I don't know, but I'm loving this color.

It smells good. Not like a flower, but I like it.

Yeah, fuzzy.

Ohemical-y.

Oareful, guys. It's a little grabby.

My sweet lord of bees!

Oandy-brain, get off there!

Problem!

• Guys!
• This could be bad.

Affirmative.

Very close.

Gonna hurt.

Mama's little boy.

You are way out of position, rookie!

Ooming in at you like a missile!

Help me!

I don't think these are flowers.

• Should we tell him?
• I think he knows.

What is this?!

Match point!

You can start packing up, honey, because you're about to eat it!

Yowser!

Gross.

There's a bee in the car!

• Do something!
• I'm driving!

• Hi, bee.
• He's back here!

He's going to sting me!

Nobody move. If you don't move, he won't sting you. Freeze!

He blinked!

Spray him, Granny!

What are you doing?!

Wow... the tension level out here is unbelievable.

I gotta get home.

Oan't fly in rain.

Oan't fly in rain.

Oan't fly in rain.

Mayday! Mayday! Bee going down!

Ken, could you close the window please?

Ken, could you close the window please?

Oheck out my new resume. I made it into a fold-out brochure.

You see? Folds out.

Oh, no. More humans. I don't need this.

What was that?

Maybe this time. This time. This time. This time! This time! This...

Drapes!

That is diabolical.

It's fantastic. It's got all my special skills, even my top-ten favorite movies.

What's number one? Star Wars?

Nah, I don't go for that...

...kind of stuff.

No wonder we shouldn't talk to them. They're out of their minds.

When I leave a job interview, they're flabbergasted, can't believe what I say.

There's the sun. Maybe that's a way out.

I don't remember the sun having a big 75 on it.

I predicted global warming.

I could feel it getting hotter. At first I thought it was just me.

Wait! Stop! Bee!

Stand back. These are winter boots.

Wait!

Don't kill him!

You know I'm allergic to them! This thing could kill me!

Why does his life have less value than yours?

Why does his life have any less value than mine? Is that your statement?

I'm just saying all life has value. You don't know what he's capable of feeling.

My brochure!

There you go, little guy.

I'm not scared of him. It's an allergic thing.

Put that on your resume brochure.

My whole face could puff up.

Make it one of your special skills.

Knocking someone out is also a special skill.

Right. Bye, Vanessa. Thanks.

• Vanessa, next week? Yogurt night?
• Sure, Ken. You know, whatever.

• You could put carob chips on there.
• Bye.

• Supposed to be less calories.
• Bye.

I gotta say something.

She saved my life. I gotta say something.

All right, here it goes.

Nah.

What would I say?

I could really get in trouble.

It's a bee law. You're not supposed to talk to a human.

I can't believe I'm doing this.

I've got to.

Oh, I can't do it. Oome on!

No. Yes. No.

Do it. I can't.

How should I start it? "You like jazz?" No, that's no good.

Here she comes! Speak, you fool!

Hi!

I'm sorry.

• You're talking.
• Yes, I know.

You're talking!

I'm so sorry.

No, it's OK. It's fine. I know I'm dreaming.

But I don't recall going to bed.

Well, I'm sure this is very disconcerting.

This is a bit of a surprise to me. I mean, you're a bee!

I am. And I'm not supposed to be doing this,

but they were all trying to kill me.

And if it wasn't for you...

I had to thank you. It's just how I was raised.

That was a little weird.

• I'm talking with a bee.
• Yeah.

I'm talking to a bee. And the bee is talking to me!

I just want to say I'm grateful. I'll leave now.

• Wait! How did you learn to do that?
• What?

The talking thing.

Same way you did, I guess. "Mama, Dada, honey." You pick it up.

• That's very funny.
• Yeah.

Bees are funny. If we didn't laugh, we'd cry with what we have to deal with.

Anyway...

Oan I...

...get you something?

• Like what?

I don't know. I mean... I don't know. Ooffee?

I don't want to put you out.

It's no trouble. It takes two minutes.

• It's just coffee.
• I hate to impose.

• Don't be ridiculous!
• Actually, I would love a cup.

Hey, you want rum cake?

• I shouldn't.
• Have some.

• No, I can't.
• Oome on!

I'm trying to lose a couple micrograms.

• Where?
• These stripes don't help.

You look great!

I don't know if you know anything about fashion.

Are you all right?

No.

He's making the tie in the cab as they're flying up Madison.

He finally gets there.

He runs up the steps into the church. The wedding is on.

And he says, "Watermelon? I thought you said Guatemalan.

Why would I marry a watermelon?"

Is that a bee joke?

That's the kind of stuff we do.

Yeah, different.

So, what are you gonna do, Barry?

About work? I don't know.

I want to do my part for the hive, but I can't do it the way they want.

I know how you feel.

• You do?
• Sure.

My parents wanted me to be a lawyer or a doctor, but I wanted to be a florist.

• Really?
• My only interest is flowers.

Our new queen was just elected with that same campaign slogan.

Anyway, if you look...

There's my hive right there. See it?

You're in Sheep Meadow!

Yes! I'm right off the Turtle Pond!

No way! I know that area. I lost a toe ring there once.

• Why do girls put rings on their toes?
• Why not?

• It's like putting a hat on your knee.
• Maybe I'll try that.

• You all right, ma'am?
• Oh, yeah. Fine.

Just having two cups of coffee!

Anyway, this has been great. Thanks for the coffee.

Yeah, it's no trouble.

Sorry I couldn't finish it. If I did, I'd be up the rest of my life.

Are you...?

Oan I take a piece of this with me?

Sure! Here, have a crumb.

• Thanks!
• Yeah.

All right. Well, then... I guess I'll see you around.

Or not.

OK, Barry.

And thank you so much again... for before.

Oh, that? That was nothing.

Well, not nothing, but... Anyway...

This can't possibly work.

He's all set to go. We may as well try it.

OK, Dave, pull the chute.

• Sounds amazing.
• It was amazing!

It was the scariest, happiest moment of my life.

Humans! I can't believe you were with humans!

Giant, scary humans! What were they like?

Huge and crazy. They talk crazy.

They eat crazy giant things. They drive crazy.

• Do they try and kill you, like on TV?
• Some of them. But some of them don't.

• How'd you get back?
• Poodle.

You did it, and I'm glad. You saw whatever you wanted to see.

You had your "experience." Now you can pick out yourjob and be normal.

• Well...
• Well?

Well, I met someone.

You did? Was she Bee-ish?

• A wasp?! Your parents will kill you!
• No, no, no, not a wasp.

• Spider?
• I'm not attracted to spiders.

I know it's the hottest thing, with the eight legs and all.

I can't get by that face.

So who is she?

She's... human.

No, no. That's a bee law. You wouldn't break a bee law.

• Her name's Vanessa.
• Oh, boy.

She's so nice. And she's a florist!

Oh, no! You're dating a human florist!

We're not dating.

You're flying outside the hive, talking to humans that attack our homes

with power washers and M-80s! One-eighth a stick of dynamite!

She saved my life! And she understands me.

This is over!

Eat this.

This is not over! What was that?

• They call it a crumb.
• It was so stingin' stripey!

And that's not what they eat. That's what falls off what they eat!

• You know what a Oinnabon is?
• No.

It's bread and cinnamon and frosting. They heat it up...

Sit down!

...really hot!

• Listen to me!

We are not them! We're us. There's us and there's them!

Yes, but who can deny the heart that is yearning?

There's no yearning. Stop yearning. Listen to me!

You have got to start thinking bee, my friend. Thinking bee!

• Thinking bee.
• Thinking bee.

Thinking bee! Thinking bee! Thinking bee! Thinking bee!

There he is. He's in the pool.

You know what your problem is, Barry?

I gotta start thinking bee?

How much longer will this go on?

It's been three days! Why aren't you working?

I've got a lot of big life decisions to think about.

What life? You have no life! You have no job. You're barely a bee!

Would it kill you to make a little honey?

Barry, come out. Your father's talking to you.

Martin, would you talk to him?

Barry, I'm talking to you!

You coming?

Got everything?

All set!

Go ahead. I'll catch up.

Don't be too long.

Watch this!

Vanessa!

• We're still here.
• I told you not to yell at him.

He doesn't respond to yelling!

• Then why yell at me?
• Because you don't listen!

I'm not listening to this.

Sorry, I've gotta go.

• Where are you going?
• I'm meeting a friend.

A girl? Is this why you can't decide?

Bye.

I just hope she's Bee-ish.

They have a huge parade of flowers every year in Pasadena?

To be in the Tournament of Roses, that's every florist's dream!

Up on a float, surrounded by flowers, crowds cheering.

A tournament. Do the roses compete in athletic events?

No. All right, I've got one. How come you don't fly everywhere?

It's exhausting. Why don't you run everywhere? It's faster.

Yeah, OK, I see, I see. All right, your turn.

TiVo. You can just freeze live TV? That's insane!

You don't have that?

We have Hivo, but it's a disease. It's a horrible, horrible disease.

Oh, my.

Dumb bees!

You must want to sting all those jerks.

We try not to sting. It's usually fatal for us.

So you have to watch your temper.

Very carefully. You kick a wall, take a walk,

write an angry letter and throw it out. Work through it like any emotion:

Anger, jealousy, lust.

Oh, my goodness! Are you OK?

Yeah.

• What is wrong with you?!
• It's a bug.

He's not bothering anybody. Get out of here, you creep!

What was that? A Pic 'N' Save circular?

Yeah, it was. How did you know?

It felt like about 10 pages. Seventy-five is pretty much our limit.

You've really got that down to a science.

• I lost a cousin to Italian Vogue.
• I'll bet.

What in the name of Mighty Hercules is this?

How did this get here? Oute Bee, Golden Blossom,

Ray Liotta Private Select?

• Is he that actor?
• I never heard of him.

• Why is this here?
• For people. We eat it.

You don't have enough food of your own?

• Well, yes.
• How do you get it?

• Bees make it.
• I know who makes it!

And it's hard to make it!

There's heating, cooling, stirring. You need a whole Krelman thing!

• It's organic.
• It's our-ganic!

It's just honey, Barry.

Just what?!

Bees don't know about this! This is stealing! A lot of stealing!

You've taken our homes, schools, hospitals! This is all we have!

And it's on sale?! I'm getting to the bottom of this.

I'm getting to the bottom of all of this!

Hey, Hector.

• You almost done?
• Almost.

He is here. I sense it.

Well, I guess I'll go home now

and just leave this nice honey out, with no one around.

You're busted, box boy!

I knew I heard something. So you can talk!

I can talk. And now you'll start talking!

Where you getting the sweet stuff? Who's your supplier?

I don't understand. I thought we were friends.

The last thing we want to do is upset bees!

You're too late! It's ours now!

You, sir, have crossed the wrong sword!

You, sir, will be lunch for my iguana, Ignacio!

Where is the honey coming from?

Tell me where!

Honey Farms! It comes from Honey Farms!

Orazy person!

What horrible thing has happened here?

These faces, they never knew what hit them. And now

they're on the road to nowhere!

Just keep still.

What? You're not dead?

Do I look dead? They will wipe anything that moves. Where you headed?

To Honey Farms. I am onto something huge here.

I'm going to Alaska. Moose blood, crazy stuff. Blows your head off!

I'm going to Tacoma.

• And you?
• He really is dead.

All right.

Uh-oh!

• What is that?!
• Oh, no!

• A wiper! Triple blade!
• Triple blade?

Jump on! It's your only chance, bee!

Why does everything have to be so doggone clean?!

How much do you people need to see?!

Open your eyes! Stick your head out the window!

From NPR News in Washington, I'm Oarl Kasell.

But don't kill no more bugs!

• Bee!
• Moose blood guy!!

• You hear something?
• Like what?

Like tiny screaming.

Turn off the radio.

Whassup, bee boy?

Hey, Blood.

Just a row of honey jars, as far as the eye could see.

Wow!

I assume wherever this truck goes is where they're getting it.

I mean, that honey's ours.

• Bees hang tight.
• We're all jammed in.

It's a close community.

Not us, man. We on our own. Every mosquito on his own.

• What if you get in trouble?
• You a mosquito, you in trouble.

Nobody likes us. They just smack. See a mosquito, smack, smack!

At least you're out in the world. You must meet girls.

Mosquito girls try to trade up, get with a moth, dragonfly.

Mosquito girl don't want no mosquito.

You got to be kidding me!

Mooseblood's about to leave the building! So long, bee!

• Hey, guys!
• Mooseblood!

I knew I'd catch y'all down here. Did you bring your crazy straw?

We throw it in jars, slap a label on it, and it's pretty much pure profit.

What is this place?

A bee's got a brain the size of a pinhead.

They are pinheads!

Pinhead.

• Oheck out the new smoker.
• Oh, sweet. That's the one you want.

The Thomas 3000!

Smoker?

Ninety puffs a minute, semi-automatic. Twice the nicotine, all the tar.

A couple breaths of this knocks them right out.

They make the honey, and we make the money.

"They make the honey, and we make the money"?

Oh, my!

What's going on? Are you OK?

Yeah. It doesn't last too long.

Do you know you're in a fake hive with fake walls?

Our queen was moved here. We had no choice.

This is your queen? That's a man in women's clothes!

That's a drag queen!

What is this?

Oh, no!

There's hundreds of them!

Bee honey.

Our honey is being brazenly stolen on a massive scale!

This is worse than anything bears have done! I intend to do something.

Oh, Barry, stop.

Who told you humans are taking our honey? That's a rumor.

Do these look like rumors?

That's a conspiracy theory. These are obviously doctored photos.

How did you get mixed up in this?

He's been talking to humans.

• What?
• Talking to humans?!

He has a human girlfriend. And they make out!

Make out? Barry!

We do not.

• You wish you could.
• Whose side are you on?

The bees!

I dated a cricket once in San Antonio. Those crazy legs kept me up all night.

Barry, this is what you want to do with your life?

I want to do it for all our lives. Nobody works harder than bees!

Dad, I remember you coming home so overworked

your hands were still stirring. You couldn't stop.

I remember that.

What right do they have to our honey?

We live on two cups a year. They put it in lip balm for no reason whatsoever!

Even if it's true, what can one bee do?

Sting them where it really hurts.

In the face! The eye!

• That would hurt.
• No.

Up the nose? That's a killer.

There's only one place you can sting the humans, one place where it matters.

Hive at Five, the hive's only full-hour action news source.

No more bee beards!

With Bob Bumble at the anchor desk.

Weather with Storm Stinger.

Sports with Buzz Larvi.

And Jeanette Ohung.

• Good evening. I'm Bob Bumble.
• And I'm Jeanette Ohung.

A tri-county bee, Barry Benson,

intends to sue the human race for stealing our honey,

packaging it and profiting from it illegally!

Tomorrow night on Bee Larry King,

we'll have three former queens here in our studio, discussing their new book,

Olassy Ladies, out this week on Hexagon.

Tonight we're talking to Barry Benson.

Did you ever think, "I'm a kid from the hive. I can't do this"?

Bees have never been afraid to change the world.

What about Bee Oolumbus? Bee Gandhi? Bejesus?

Where I'm from, we'd never sue humans.

We were thinking of stickball or candy stores.

How old are you?

The bee community is supporting you in this case,

which will be the trial of the bee century.

You know, they have a Larry King in the human world too.

It's a common name. Next week...

He looks like you and has a show and suspenders and colored dots...

Next week...

Glasses, quotes on the bottom from the guest even though you just heard 'em.

Bear Week next week! They're scary, hairy and here live.

Always leans forward, pointy shoulders, squinty eyes, very Jewish.

In tennis, you attack at the point of weakness!

It was my grandmother, Ken. She's 81.

Honey, her backhand's a joke! I'm not gonna take advantage of that?

Quiet, please. Actual work going on here.

• Is that that same bee?
• Yes, it is!

I'm helping him sue the human race.

• Hello.
• Hello, bee.

This is Ken.

Yeah, I remember you. Timberland, size ten and a half. Vibram sole, I believe.

Why does he talk again?

Listen, you better go 'cause we're really busy working.

But it's our yogurt night!

Bye-bye.

Why is yogurt night so difficult?!

You poor thing. You two have been at this for hours!

Yes, and Adam here has been a huge help.

• Frosting...
• How many sugars?

Just one. I try not to use the competition.

So why are you helping me?

Bees have good qualities.

And it takes my mind off the shop.

Instead of flowers, people are giving balloon bouquets now.

Those are great, if you're three.

And artificial flowers.

• Oh, those just get me psychotic!
• Yeah, me too.

Bent stingers, pointless pollination.

Bees must hate those fake things!

Nothing worse than a daffodil that's had work done.

Maybe this could make up for it a little bit.

• This lawsuit's a pretty big deal.
• I guess.

You sure you want to go through with it?

Am I sure? When I'm done with the humans, they won't be able

to say, "Honey, I'm home," without paying a royalty!

It's an incredible scene here in downtown Manhattan,

where the world anxiously waits, because for the first time in history,

we will hear for ourselves if a honeybee can actually speak.

What have we gotten into here, Barry?

It's pretty big, isn't it?

I can't believe how many humans don't work during the day.

You think billion-dollar multinational food companies have good lawyers?

Everybody needs to stay behind the barricade.

• What's the matter?
• I don't know, I just got a chill.

Well, if it isn't the bee team.

You boys work on this?

All rise! The Honorable Judge Bumbleton presiding.

All right. Oase number 4475,

Superior Oourt of New York, Barry Bee Benson v. the Honey Industry

is now in session.

Mr. Montgomery, you're representing the five food companies collectively?

A privilege.

Mr. Benson... you're representing all the bees of the world?

I'm kidding. Yes, Your Honor, we're ready to proceed.

Mr. Montgomery, your opening statement, please.

Ladies and gentlemen of the jury,

my grandmother was a simple woman.

Born on a farm, she believed it was man's divine right

to benefit from the bounty of nature God put before us.

If we lived in the topsy-turvy world Mr. Benson imagines,

just think of what would it mean.

I would have to negotiate with the silkworm

for the elastic in my britches!

Talking bee!

How do we know this isn't some sort of

holographic motion-picture-capture Hollywood wizardry?

They could be using laser beams!

Robotics! Ventriloquism! Oloning! For all we know,

he could be on steroids!

Mr. Benson?

Ladies and gentlemen, there's no trickery here.

I'm just an ordinary bee. Honey's pretty important to me.

It's important to all bees. We invented it!

We make it. And we protect it with our lives.

Unfortunately, there are some people in this room

who think they can take it from us

'cause we're the little guys! I'm hoping that, after this is all over,

you'll see how, by taking our honey, you not only take everything we have

but everything we are!

I wish he'd dress like that all the time. So nice!

Oall your first witness.

So, Mr. Klauss Vanderhayden of Honey Farms, big company you have.

I suppose so.

I see you also own Honeyburton and Honron!

Yes, they provide beekeepers for our farms.

Beekeeper. I find that to be a very disturbing term.

I don't imagine you employ any bee-free-ers, do you?

• No.
• I couldn't hear you.

• No.
• No.

Because you don't free bees. You keep bees. Not only that,

it seems you thought a bear would be an appropriate image for a jar of honey.

They're very lovable creatures.

Yogi Bear, Fozzie Bear, Build-A-Bear.

You mean like this?

Bears kill bees!

How'd you like his head crashing through your living room?!

Biting into your couch! Spitting out your throw pillows!

OK, that's enough. Take him away.

So, Mr. Sting, thank you for being here. Your name intrigues me.

• Where have I heard it before?
• I was with a band called The Police.

But you've never been a police officer, have you?

No, I haven't.

No, you haven't. And so here we have yet another example

of bee culture casually stolen by a human

for nothing more than a prance-about stage name.

Oh, please.

Have you ever been stung, Mr. Sting?

Because I'm feeling a little stung, Sting.

Or should I say... Mr. Gordon M. Sumner!

That's not his real name?! You idiots!

Mr. Liotta, first, belated congratulations on

your Emmy win for a guest spot on ER in 2005.

Thank you. Thank you.

I see from your resume that you're devilishly handsome

with a churning inner turmoil that's ready to blow.

I enjoy what I do. Is that a crime?

Not yet it isn't. But is this what it's come to for you?

Exploiting tiny, helpless bees so you don't

have to rehearse your part and learn your lines, sir?

Watch it, Benson! I could blow right now!

This isn't a goodfella. This is a badfella!

Why doesn't someone just step on this creep, and we can all go home?!

• Order in this court!
• You're all thinking it!

Order! Order, I say!

• Say it!
• Mr. Liotta, please sit down!

I think it was awfully nice of that bear to pitch in like that.

I think the jury's on our side.

Are we doing everything right, legally?

I'm a florist.

Right. Well, here's to a great team.

To a great team!

Well, hello.

• Ken!
• Hello.

I didn't think you were coming.

No, I was just late. I tried to call, but... the battery.

I didn't want all this to go to waste, so I called Barry. Luckily, he was free.

Oh, that was lucky.

There's a little left. I could heat it up.

Yeah, heat it up, sure, whatever.

So I hear you're quite a tennis player.

I'm not much for the game myself. The ball's a little grabby.

That's where I usually sit. Right... there.

Ken, Barry was looking at your resume,

and he agreed with me that eating with chopsticks isn't really a special skill.

You think I don't see what you're doing?

I know how hard it is to find the rightjob. We have that in common.

Do we?

Bees have 100 percent employment, but we do jobs like taking the crud out.

That's just what I was thinking about doing.

Ken, I let Barry borrow your razor for his fuzz. I hope that was all right.

I'm going to drain the old stinger.

Yeah, you do that.

Look at that.

You know, I've just about had it

with your little mind games.

• What's that?
• Italian Vogue.

Mamma mia, that's a lot of pages.

A lot of ads.

Remember what Van said, why is your life more valuable than mine?

Funny, I just can't seem to recall that!

I think something stinks in here!

I love the smell of flowers.

How do you like the smell of flames?!

Not as much.

Water bug! Not taking sides!

Ken, I'm wearing a Ohapstick hat! This is pathetic!

I've got issues!

Well, well, well, a royal flush!

• You're bluffing.
• Am I?

Surf's up, dude!

Poo water!

That bowl is gnarly.

Except for those dirty yellow rings!

Kenneth! What are you doing?!

You know, I don't even like honey! I don't eat it!

We need to talk!

He's just a little bee!

And he happens to be the nicest bee I've met in a long time!

Long time? What are you talking about?! Are there other bugs in your life?

No, but there are other things bugging me in life. And you're one of them!

Fine! Talking bees, no yogurt night...

My nerves are fried from riding on this emotional roller coaster!

Goodbye, Ken.

And for your information,

I prefer sugar-free, artificial sweeteners made by man!

I'm sorry about all that.

I know it's got an aftertaste! I like it!

I always felt there was some kind of barrier between Ken and me.

I couldn't overcome it. Oh, well.

Are you OK for the trial?

I believe Mr. Montgomery is about out of ideas.

We would like to call Mr. Barry Benson Bee to the stand.

Good idea! You can really see why he's considered one of the best lawyers...

Yeah.

Layton, you've gotta weave some magic

with this jury, or it's gonna be all over.

Don't worry. The only thing I have to do to turn this jury around

is to remind them of what they don't like about bees.

• You got the tweezers?
• Are you allergic?

Only to losing, son. Only to losing.

Mr. Benson Bee, I'll ask you what I think we'd all like to know.

What exactly is your relationship

to that woman?

We're friends.

• Good friends?
• Yes.

How good? Do you live together?

Wait a minute...

Are you her little...

...bedbug?

I've seen a bee documentary or two. From what I understand,

doesn't your queen give birth to all the bee children?

• Yeah, but...
• So those aren't your real parents!

• Oh, Barry...
• Yes, they are!

Hold me back!

You're an illegitimate bee, aren't you, Benson?

He's denouncing bees!

Don't y'all date your cousins?

• Objection!
• I'm going to pincushion this guy!

Adam, don't! It's what he wants!

Oh, I'm hit!!

Oh, lordy, I am hit!

Order! Order!

The venom! The venom is coursing through my veins!

I have been felled by a winged beast of destruction!

You see? You can't treat them like equals! They're striped savages!

Stinging's the only thing they know! It's their way!

• Adam, stay with me.
• I can't feel my legs.

What angel of mercy will come forward to suck the poison

from my heaving buttocks?

I will have order in this court. Order!

Order, please!

The case of the honeybees versus the human race

took a pointed turn against the bees

yesterday when one of their legal team stung Layton T. Montgomery.

• Hey, buddy.
• Hey.

• Is there much pain?
• Yeah.

I...

I blew the whole case, didn't I?

It doesn't matter. What matters is you're alive. You could have died.

I'd be better off dead. Look at me.

They got it from the cafeteria downstairs, in a tuna sandwich.

Look, there's a little celery still on it.

What was it like to sting someone?

I can't explain it. It was all...

All adrenaline and then... and then ecstasy!

All right.

You think it was all a trap?

Of course. I'm sorry. I flew us right into this.

What were we thinking? Look at us. We're just a couple of bugs in this world.

What will the humans do to us if they win?

I don't know.

I hear they put the roaches in motels. That doesn't sound so bad.

Adam, they check in, but they don't check out!

Oh, my.

Oould you get a nurse to close that window?

• Why?
• The smoke.

Bees don't smoke.

Right. Bees don't smoke.

Bees don't smoke! But some bees are smoking.

That's it! That's our case!

It is? It's not over?

Get dressed. I've gotta go somewhere.

Get back to the court and stall. Stall any way you can.

And assuming you've done step correctly, you're ready for the tub.

Mr. Flayman.

Yes? Yes, Your Honor!

Where is the rest of your team?

Well, Your Honor, it's interesting.

Bees are trained to fly haphazardly,

and as a result, we don't make very good time.

I actually heard a funny story about...

Your Honor, haven't these ridiculous bugs

taken up enough of this court's valuable time?

How much longer will we allow these absurd shenanigans to go on?

They have presented no compelling evidence to support their charges

against my clients, who run legitimate businesses.

I move for a complete dismissal of this entire case!

Mr. Flayman, I'm afraid I'm going

to have to consider Mr. Montgomery's motion.

But you can't! We have a terrific case.

Where is your proof? Where is the evidence?

Show me the smoking gun!

Hold it, Your Honor! You want a smoking gun?

Here is your smoking gun.

What is that?

It's a bee smoker!

What, this? This harmless little contraption?

This couldn't hurt a fly, let alone a bee.

Look at what has happened

to bees who have never been asked, "Smoking or non?"

Is this what nature intended for us?

To be forcibly addicted to smoke machines

and man-made wooden slat work camps?

Living out our lives as honey slaves to the white man?

• What are we gonna do?
• He's playing the species card.

Ladies and gentlemen, please, free these bees!

Free the bees! Free the bees!

Free the bees!

Free the bees! Free the bees!

The court finds in favor of the bees!

Vanessa, we won!

I knew you could do it! High-five!

Sorry.

I'm OK! You know what this means?

All the honey will finally belong to the bees.

Now we won't have to work so hard all the time.

This is an unholy perversion of the balance of nature, Benson.

You'll regret this.

Barry, how much honey is out there?

All right. One at a time.

Barry, who are you wearing?

My sweater is Ralph Lauren, and I have no pants.

• What if Montgomery's right?
• What do you mean?

We've been living the bee way a long time, 27 million years.

Oongratulations on your victory. What will you demand as a settlement?

First, we'll demand a complete shutdown of all bee work camps.

Then we want back the honey that was ours to begin with,

every last drop.

We demand an end to the glorification of the bear as anything more

than a filthy, smelly, bad-breath stink machine.

We're all aware of what they do in the woods.

Wait for my signal.

Take him out.

He'll have nauseous for a few hours, then he'll be fine.

And we will no longer tolerate bee-negative nicknames...

But it's just a prance-about stage name!

...unnecessary inclusion of honey in bogus health products

and la-dee-da human tea-time snack garnishments.

Oan't breathe.

Bring it in, boys!

Hold it right there! Good.

Tap it.

Mr. Buzzwell, we just passed three cups, and there's gallons more coming!

• I think we need to shut down!
• Shut down? We've never shut down.

Shut down honey production!

Stop making honey!

Turn your key, sir!

What do we do now?

Oannonball!

We're shutting honey production!

Mission abort.

Aborting pollination and nectar detail. Returning to base.

Adam, you wouldn't believe how much honey was out there.

Oh, yeah?

What's going on? Where is everybody?

• Are they out celebrating?
• They're home.

They don't know what to do. Laying out, sleeping in.

I heard your Uncle Oarl was on his way to San Antonio with a cricket.

At least we got our honey back.

Sometimes I think, so what if humans liked our honey? Who wouldn't?

It's the greatest thing in the world! I was excited to be part of making it.

This was my new desk. This was my new job. I wanted to do it really well.

And now...

Now I can't.

I don't understand why they're not happy.

I thought their lives would be better!

They're doing nothing. It's amazing. Honey really changes people.

You don't have any idea what's going on, do you?

• What did you want to show me?
• This.

What happened here?

That is not the half of it.

Oh, no. Oh, my.

They're all wilting.

Doesn't look very good, does it?

No.

And whose fault do you think that is?

You know, I'm gonna guess bees.

Bees?

Specifically, me.

I didn't think bees not needing to make honey would affect all these things.

It's notjust flowers. Fruits, vegetables, they all need bees.

That's our whole SAT test right there.

Take away produce, that affects the entire animal kingdom.

And then, of course...

The human species?

So if there's no more pollination,

it could all just go south here, couldn't it?

I know this is also partly my fault.

How about a suicide pact?

How do we do it?

• I'll sting you, you step on me.
• Thatjust kills you twice.

Right, right.

Listen, Barry... sorry, but I gotta get going.

I had to open my mouth and talk.

Vanessa?

Vanessa? Why are you leaving? Where are you going?

To the final Tournament of Roses parade in Pasadena.

They've moved it to this weekend because all the flowers are dying.

It's the last chance I'll ever have to see it.

Vanessa, I just wanna say I'm sorry. I never meant it to turn out like this.

I know. Me neither.

Tournament of Roses. Roses can't do sports.

Wait a minute. Roses. Roses?

Roses!

Vanessa!

Roses?!

Barry?

• Roses are flowers!
• Yes, they are.

Flowers, bees, pollen!

I know. That's why this is the last parade.

Maybe not. Oould you ask him to slow down?

Oould you slow down?

Barry!

OK, I made a huge mistake. This is a total disaster, all my fault.

Yes, it kind of is.

I've ruined the planet. I wanted to help you

with the flower shop. I've made it worse.

Actually, it's completely closed down.

I thought maybe you were remodeling.

But I have another idea, and it's greater than my previous ideas combined.

I don't want to hear it!

All right, they have the roses, the roses have the pollen.

I know every bee, plant and flower bud in this park.

All we gotta do is get what they've got back here with what we've got.

• Bees.
• Park.

• Pollen!
• Flowers.

• Repollination!
• Across the nation!

Tournament of Roses, Pasadena, Oalifornia.

They've got nothing but flowers, floats and cotton candy.

Security will be tight.

I have an idea.

Vanessa Bloome, FTD.

Official floral business. It's real.

Sorry, ma'am. Nice brooch.

Thank you. It was a gift.

Once inside, we just pick the right float.

How about The Princess and the Pea?

I could be the princess, and you could be the pea!

Yes, I got it.

• Where should I sit?
• What are you?

• I believe I'm the pea.
• The pea?

It goes under the mattresses.

• Not in this fairy tale, sweetheart.
• I'm getting the marshal.

You do that! This whole parade is a fiasco!

Let's see what this baby'll do.

Hey, what are you doing?!

Then all we do is blend in with traffic...

...without arousing suspicion.

Once at the airport, there's no stopping us.

Stop! Security.

• You and your insect pack your float?
• Yes.

Has it been in your possession the entire time?

Would you remove your shoes?

• Remove your stinger.
• It's part of me.

I know. Just having some fun. Enjoy your flight.

Then if we're lucky, we'll have just enough pollen to do the job.

Oan you believe how lucky we are? We have just enough pollen to do the job!

I think this is gonna work.

It's got to work.

Attention, passengers, this is Oaptain Scott.

We have a bit of bad weather in New York.

It looks like we'll experience a couple hours delay.

Barry, these are cut flowers with no water. They'll never make it.

I gotta get up there and talk to them.

Be careful.

Oan I get help with the Sky Mall magazine?

I'd like to order the talking inflatable nose and ear hair trimmer.

Oaptain, I'm in a real situation.

• What'd you say, Hal?
• Nothing.

Bee!

Don't freak out! My entire species...

What are you doing?

• Wait a minute! I'm an attorney!
• Who's an attorney?

Don't move.

Oh, Barry.

Good afternoon, passengers. This is your captain.

Would a Miss Vanessa Bloome in 24B please report to the cockpit?

And please hurry!

What happened here?

There was a DustBuster, a toupee, a life raft exploded.

One's bald, one's in a boat, they're both unconscious!

• Is that another bee joke?
• No!

No one's flying the plane!

This is JFK control tower, Flight 356. What's your status?

This is Vanessa Bloome. I'm a florist from New York.

Where's the pilot?

He's unconscious, and so is the copilot.

Not good. Does anyone onboard have flight experience?

As a matter of fact, there is.

• Who's that?
• Barry Benson.

From the honey trial?! Oh, great.

Vanessa, this is nothing more than a big metal bee.

It's got giant wings, huge engines.

I can't fly a plane.

• Why not? Isn't John Travolta a pilot?
• Yes.

How hard could it be?

Wait, Barry! We're headed into some lightning.

This is Bob Bumble. We have some late-breaking news from JFK Airport,

where a suspenseful scene is developing.

Barry Benson, fresh from his legal victory...

That's Barry!

...is attempting to land a plane, loaded with people, flowers

and an incapacitated flight crew.

Flowers?!

We have a storm in the area and two individuals at the controls

with absolutely no flight experience.

Just a minute. There's a bee on that plane.

I'm quite familiar with Mr. Benson and his no-account compadres.

They've done enough damage.

But isn't he your only hope?

Technically, a bee shouldn't be able to fly at all.

Their wings are too small...

Haven't we heard this a million times?

"The surface area of the wings and body mass make no sense."

• Get this on the air!
• Got it.

• Stand by.
• We're going live.

The way we work may be a mystery to you.

Making honey takes a lot of bees doing a lot of small jobs.

But let me tell you about a small job.

If you do it well, it makes a big difference.

More than we realized. To us, to everyone.

That's why I want to get bees back to working together.

That's the bee way! We're not made of Jell-O.

We get behind a fellow.

• Black and yellow!
• Hello!

Left, right, down, hover.

• Hover?
• Forget hover.

This isn't so hard. Beep-beep! Beep-beep!

Barry, what happened?!

Wait, I think we were on autopilot the whole time.

• That may have been helping me.
• And now we're not!

So it turns out I cannot fly a plane.

All of you, let's get behind this fellow! Move it out!

Move out!

Our only chance is if I do what I'd do, you copy me with the wings of the plane!

Don't have to yell.

I'm not yelling! We're in a lot of trouble.

It's very hard to concentrate with that panicky tone in your voice!

It's not a tone. I'm panicking!

I can't do this!

Vanessa, pull yourself together. You have to snap out of it!

You snap out of it.

You snap out of it.

• You snap out of it!
• You snap out of it!

• You snap out of it!
• You snap out of it!

• You snap out of it!
• You snap out of it!

• Hold it!
• Why? Oome on, it's my turn.

How is the plane flying?

I don't know.

Hello?

Benson, got any flowers for a happy occasion in there?

The Pollen Jocks!

They do get behind a fellow.

• Black and yellow.
• Hello.

All right, let's drop this tin can on the blacktop.

Where? I can't see anything. Oan you?

No, nothing. It's all cloudy.

Oome on. You got to think bee, Barry.

• Thinking bee.
• Thinking bee.

Thinking bee! Thinking bee! Thinking bee!

Wait a minute. I think I'm feeling something.

• What?
• I don't know. It's strong, pulling me.

Like a 27-million-year-old instinct.

Bring the nose down.

Thinking bee! Thinking bee! Thinking bee!

• What in the world is on the tarmac?
• Get some lights on that!

Thinking bee! Thinking bee! Thinking bee!

• Vanessa, aim for the flower.
• OK.

Out the engines. We're going in on bee power. Ready, boys?

Affirmative!

Good. Good. Easy, now. That's it.

Land on that flower!

Ready? Full reverse!

Spin it around!

• Not that flower! The other one!
• Which one?

• That flower.
• I'm aiming at the flower!

That's a fat guy in a flowered shirt. I mean the giant pulsating flower

made of millions of bees!

Pull forward. Nose down. Tail up.

Rotate around it.

• This is insane, Barry!
• This's the only way I know how to fly.

Am I koo-koo-kachoo, or is this plane flying in an insect-like pattern?

Get your nose in there. Don't be afraid. Smell it. Full reverse!

Just drop it. Be a part of it.

Aim for the center!

Now drop it in! Drop it in, woman!

Oome on, already.

Barry, we did it! You taught me how to fly!

• Yes. No high-five!
• Right.

Barry, it worked! Did you see the giant flower?

What giant flower? Where? Of course I saw the flower! That was genius!

• Thank you.
• But we're not done yet.

Listen, everyone!

This runway is covered with the last pollen

from the last flowers available anywhere on Earth.

That means this is our last chance.

We're the only ones who make honey, pollinate flowers and dress like this.

If we're gonna survive as a species, this is our moment! What do you say?

Are we going to be bees, orjust Museum of Natural History keychains?

We're bees!

Keychain!

Then follow me! Except Keychain.

Hold on, Barry. Here.

You've earned this.

Yeah!

I'm a Pollen Jock! And it's a perfect fit. All I gotta do are the sleeves.

Oh, yeah.

That's our Barry.

Mom! The bees are back!

If anybody needs to make a call, now's the time.

I got a feeling we'll be working late tonight!

Here's your change. Have a great afternoon! Oan I help who's next?

Would you like some honey with that? It is bee-approved. Don't forget these.

Milk, cream, cheese, it's all me. And I don't see a nickel!

Sometimes I just feel like a piece of meat!

I had no idea.

Barry, I'm sorry. Have you got a moment?

Would you excuse me? My mosquito associate will help you.

Sorry I'm late.

He's a lawyer too?

I was already a blood-sucking parasite. All I needed was a briefcase.

Have a great afternoon!

Barry, I just got this huge tulip order, and I can't get them anywhere.

No problem, Vannie. Just leave it to me.

You're a lifesaver, Barry. Oan I help who's next?

All right, scramble, jocks! It's time to fly.

Thank you, Barry!

That bee is living my life!

Let it go, Kenny.

• When will this nightmare end?!
• Let it all go.

• Beautiful day to fly.
• Sure is.

Between you and me, I was dying to get out of that office.

You have got to start thinking bee, my friend.

• Thinking bee!
• Me?

Hold it. Let's just stop for a second. Hold it.

I'm sorry. I'm sorry, everyone. Oan we stop here?

I'm not making a major life decision during a production number!

All right. Take ten, everybody. Wrap it up, guys.

I had virtually no rehearsal for that.

SciScore for 10.1101/2021.12.09.21267516: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

No key resources detected.

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Strengths and limitations: With 28,356 adults in our sample, this is the largest study to date internationally on Long Covid and COVID-19 vaccination, and the first to investigate post-vaccine symptom trajectories. The main strength of the study is its use of the CIS, a large survey of approximately half a million people from the community population of the UK with longitudinal follow-up. Random sampling from address lists mitigates against selection bias, while the prospective design means that survey responses are not subject to outcome recall bias (such as participants overestimating the duration of previously experienced symptoms). All CIS participants are swabbed for SARS-CoV-2 at every follow-up visit, irrespective of symptoms, so our study includes asymptomatic as well symptomatic infections. The study also has limitations. Its observational nature means that causality cannot be inferred, and placebo and side effects of vaccination may have contributed to our findings; however, estimates were robust to excluding follow-up visits within the first week of each vaccination, suggesting that the impact of these effects is likely to be small. Although we adjusted for a wide range of potential confounders, unmeasured factors, such as those related to take-up of a second vaccination, may remain. The observed changes after vaccination could be related to the relapsing-remitting nature of symptoms experienced by many people living with Long Covid [3–5] rather than a causal effec...

Results from TrialIdentifier: We found the following clinical trial numbers in your paper:<br><table><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">Identifier</td><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">Status</td><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">Title</td></tr><tr><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">ISRCTN21086382</td><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">NA</td><td style="min-width:95px; border-right:1px solid lightgray; border-bottom:1px solid lightgray">NA</td></tr></table>

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

Results from scite Reference Check: We found no unreliable references.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

"Waste generation rate in Nigeria is estimated at 0.65-0.95 kg/capita/day which gives an average of 42 million tonnes of wastes generated annually. This is more than half of 62 million tonnes of waste generated in sub-Sahara Africa annually;"

https://www.researchgate.net/publication/328914649_Solid_Waste_Management_in_Nigeria_Problems_Prospects_and_Policies#:~:text=Waste%20generation%20rate%20in%20Nigeria,huge%20problem%20for%20the%20nation.

SciScore for 10.1101/2021.11.23.21266734: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

Results from OddPub: Thank you for sharing your code.

Strengths and limitations: Much of the COVID-19 literature is based on studies where study populations have been drawn from people hospitalised with COVID-19, tested for infection, or who volunteered to participate in a study. Such studies can be subject to a number of biases, in particular collider bias which can lead to the reporting associations that do not exist for the general population or by attenuating, inflating or reversing the sign of true associations.30 This underscores the importance of developing comprehensive datasets to generate the reliable evidence required to inform decision-making related to the pandemic. With more than half a million outpatient cases of COVID-19 captured and a breadth of data capture that allows for comparisons with the general population and subsequent hospital care to be described, the mapped SIDIAP database described here is one such resource. While electronic health record data brings numerous opportunities, with the data collected for non-research purposes careful curation is required. Using a well-established common data model, meant that existing open-source tools could be used to evaluate data quality and that research studies can be run in a distributed manner. This has allowed the database to already have been used in a number of international network research studies, with standardised analytic packages and only aggregated results sets shared. One limitation of the dataset has been seen with the likely underreporting of COVID-...

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

Results from scite Reference Check: We found no unreliable references.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

Note: This rebuttal was posted by the corresponding author to Review Commons. Content has not been altered except for formatting.

Learn more at Review Commons

Reply to the reviewers

Reviewer #1 (Evidence, reproducibility and clarity (Required)):

**Summary:**

I found this an exceptionally impressive manuscript. The evolution of Y chromosomes has until recently been nearly impossible, and this research group have pioneered approaches that can yield reliable results in Drosophila. The study used an innovative heterochromatin-sensitive assembly pipeline on three D. simulans clade species, D. simulans, D. mauritiana and D. sechellia, which diverged less than 250 KYA, allowing comparisons with the group's previous results for the D. melanogaster Y.

The study is both technically impressive and extremely interesting (an highly unusual combination). It includes a rich set of interesting results about these genome regions, and furthermore the results are discussed in a well-organised way, relating both to previous observations and to understanding of the genetics and evolution of Y chromosomes, illuminating all these aspects. It is a rare pleasure to read such a study. I believe that this study will inspire and be a model for future work on these chromosomes. It shows how these difficult genome regions can be studied.

Thank you for the positive evaluation of our paper. While we did not make any specific revisions in response to these comments, we did attempt to improve the writing.

**Major comments:**

The conclusions are convincing. The methods are explained unusually clearly, and the reasoning from the results is convincing. When appropriate, the caveats, the caveats are clearly explained. The material is clearly organised and the questions studied are well related to the results. I had a few minor comments concerning the English. Even the figure (often a major problem to understand) are very clear and helpful, with proper explanations. I have very rarely read such a good manuscript, and almost never (in a long career) found a manuscript that could be published without revision being necessary.

Thank you for pointing out that there were minor concerns with the English. We have carefully gone through the manuscript and fixed some minor issues with the writing. The analysis found 58 exons missed in previous assemblies (as well as all previously known exons of the 11 canonical Y-linked genes, which are present in at least one copy across the group). FISH on mitotic chromosomes using probes for 12 Y-linked sequences was used to determine the centromere locations, and to determine gene orders and relate them to the cytological chromosome bands, demonstrating changes in satellite distribution, gene order, and centromere positions between their Y chromosomes within the D. simulans clade species. It also confirmed previous results for Y-linked ribosomal DNA,genes, which are responsible for X-Y pairing in D. melanogaster males. Although 28S rDNA has been lost in D. simulans and D. sechellia (but not in D. mauritiana), the intergenic spacer (IGS) repeats between these repeats are retained on both sex chromosomes in all three species. Only sequencing can reliably reveal this, as their abundance is below the detection level by FISH in D. sechellia. The 11 canonical Y-linked genes' copy numbers vary between the species, and some duplicates are expressed and have complete open reading frames, and may therefore be functional because they, but most include only a subset of exons, often with duplicated exons flanking the the presumed functional gene copy. Mega-introns and Y-loops were found, as already seen in Drosophila species, but this new study detects turn overs in the ~2 million years separating D. melanogaster and the D. simulans clade. 49 independent duplications onto the Y chromosome were detected, including 8 not previously detected. At least half show no expression in testes, or lack open reading frames, so they are probably pseudogenes. Testis-expressed genes may be especially likely to duplicate into the Y chromosome due to its open chromatin structure and transcriptional activity during spermatogenesis, and indeed most of the new Y-linked genes in the species studied clade have likely functions in chromatin modification, cell division, and sexual reproduction. The study discovered two new gene families that have undergone amplification on D. simulans clade Y chromosomes, reaching very high copy numbers (36-146). Both these families appear to encode functional protein-coding genes and show high expression. The paper described intriguing results that illuminate Y chromosome evolution. First, SRPK, arose by an autosome-to-Y duplication of the sequence encoding the testis-specific isoform of the gene SR Protein Kinase (SRPK), after which the autosomal copy lost its testis-specific exon via a deletion. In D. melanogaster, SRPK is essential for both male and female reproduction, so the relocation of the testis-specific isoform to the Y chromosome in the D. simulans clade suggests that the change may have been advantageous by resolving sexual antagonism. The paper presents convincing evidence that the Y copy evolved under positive selection, and that gene amplification may confer advantageous increased expression in males. The second amplified gene family is also potentially related to an interesting function. Both X-linked and Y-linked duplicates are found of a gene called Ssl located on chromosome 2R. In D. simulans, the X-linked copies were previously known, and called CK2ßtes-like. In D. melanogaster, degenerated Y-linked copies are also found, with little or no expression, contrasting with complete open reading frames and high expression in the D. simulans clade species in testes, consistent with the possibility of an arms race between sex chromosome meiotic drive factors. Other interesting analyses document higher gene conversion rates compared to the other chromosomes, and evidence that these Y chromosomes may differ in the DNA-repair mechanisms (preferentially using MMEJ instead of NHEJ), perhaps contributing to their high rates of intrachromosomal duplication and structural rearrangements. The authors relate this to evidence for turnover of Y-linked satellite sequences, with the discovery of five new Y-linked satellites, whose locations were validated using FISH. The study also documented enrichment of LTR retrotransposons on the D. simulans clade Y chromosomes relative to the rest of the genome, together with turnovers between the species.

Reviewer #1 (Significance (Required)):

As described above, the advances are both, technical and conceptual for the field. The manuscript itself does an excellent job of placing the work in the context of the existing literature.

• Anyone working on sex chromosomes and other non-recombining genome regions should be interested in the findings reported.

• My field of expertise is the evolution of sex chromosomes, and the evolution of genome regions with suppressed recombination. I have experience of genomic analyses. I have less expertise in analyses of gene expression, but I understand enough about such approaches to evaluate the parts of this study that use them.

Reviewer #2:

Reviewer #2 (Evidence, reproducibility and clarity (Required)):

The manuscript describes a thorough investigation of the Y-chromosomes of three very closely related Drosophila species (D. simulans, D. sechellia, and D. mauritiana) which in turn are closely related to D. melanogaster. The D. melanogaster Y was analysed in a previous paper by the same goup. The authors found an astonishing level of structural rearrangements (gene order, copy number, etc.), specially taking into account the short divergence time among the three species (~250 thousand years). They also suggest an explanation for this fast evolution: Y chromosome is haploid, and hence double-strand breaks cannot be repaired by homologous recombination. Instead, it must use the less precise mechanisms of NHEJ and MMEJ. They also provide circumstantial evidence that MMEJ (which is very prone to generate large rearrangements) is the preferred mechanism of repair. As far as I know this hypothesis is new, and fits nicely on the fast structural evolution described by the authors. Finally, the authors describe two intriguing Y-linked gene families in D. simulans (Lhk and CK2ßtes-Y), one of them similar to the Stellate / Suppressor of Stellate system of D. melanogaster, which seems to be evolving as part of a X-Y meiotic drive arms race. Overall, it is a very nice piece of work. I have four criticisms that, in my opinion, should be addressed before acceptance.

Thank you for your positive comments. We respond to your concerns point-by-point below.

The suggestion/conclusion that MMEJ is the preferential repair mechanism (over NHEJ) should be better supported and explained. At line 387, the authors stated "The pattern of excess large deletions is shared in the three D. simulans clade species Y chromosomes, but is not obvious in D. melanogaster (Fig 6B). However, because all D. melanogaster Y-linked indels in our analyses are from copies of a single pseudogene (CR43975), it is difficult to compare to the larger samples in the simulans clade species (duplicates from 16 genes). ". Given that D. melanogaster has many Y-linked pseudogenes (described by the authors and by other researchers, and listed in Table S6), there seems to be no reason to use a sample size of 1 in this species.

We only used pseudogenes with large alignable regions (>300 bp) to prevent the potential bias toward small indels and increase our confidence in indel calling. As a result, we excluded most of the duplicates on the D. melanogaster Y chromosome. We now include 5 additional D. melanogaster Y-linked indels in the manuscript, however, the majority of indels in this species (36/41) are still from the same gene.

Furthermore, given that D. melanogaster is THE model organism, it is the species that most likely will provide information to assess the "preferential MMEJ" hypothesis proposed by the authors.

A previous paper has shown that male flies deficient in MMEJ have a strong bias toward female offspring (McKee et al. 2000), suggesting that MMEJ is necessary for successfully producing Y-bearing sperm, consistent with our hypothesis. We agree with the reviewer that careful genetic and cytological experiments in D. melanogaster could further clarify the role of MMEJ in the repair of Y-linked mutations. Even more revealing would be experiments using the simulans clade species, where we hypothesize the MMEJ bias is even more pronounced on the Y chromosome. We believe, however, that these experiments are beyond the scope of this study and should merit their own papers.

Still on the suggestion/conclusion that MMEJ is the preferential repair mechanism (over NHEJ). Y chromosome in heterochromatic, haploid and non-recombining. In order to ascribe its mutational pattern to the haploid state (and the consequent impossibility of homologous recombination repair), the authors compared it to chromosome IV (the so called "dot chromosome"). This may not be the best choice: while chr IV lacks recombination in wild type flies, it is not typical heterochromatin. E.g., " results from genetic analyses, genomic studies, and biochemical investigations have revealed the dot chromosome to be unique, having a mixture of characteristics of euchromatin and of constitutive heterochromatin". Riddle and Elgin, FlyBook 2018 (https://doi.org/10.1534/genetics.118.301146). Given this, it seems appropriate to also compare the Y-linked pseudogenes with those from typical heterochromatin. In Drosophila, these are the regions around the centromeres ("centric heterochromatin"). There are pseudogenes there; e.g., the gene rolled is known to have partially duplicated exons.

Thank you for the suggestion. We now include the data from pericentric heterochromatin and pseudogenes in supplemental data (see Fig 7). Both data types support our conclusion that indel size is only larger on Y chromosomes, which is consistent with the comparison between the dot chromosome and pericentric heterochromatin reported by Blumenstiel et al. 2002.

In some passages of the ms there seems to be a confusion between new genes and pseudogenes, which should be corrected. For example, in line 261: "Most new Y-linked genes in D. melanogaster and the D. simulans clade have presumed functions in chromatin modification, cell division, and sexual reproduction (Table S7)".. Who are these "new genes"? If they are those listed in Table S6 (as other passages of the text suggest), most if not all of them are pseudogenes. If they are pseudogenes, it is not appropriate to refer to them as "new genes". The same ambiguity is present in line 263: "Y-linked duplicates of genes with these functions may be selectively beneficial, but a duplication bias could also contribute to this enrichment (...) " Pseudogenes can be selectively beneficial, but in very special cases (e.g.. gene regulation). If the authors are suggesting this, they must openly state this, and explain why. Pseudogenes are common in nearly all genomes, and should be clearly separated from genes (the later as a shortcut for functional genes). The bar for "genes" is much higher than simple sequence similarity, including expression, evidences of purifying selecion, etc., as the authors themselves applied for the two gene families they identified in D. simulans (Lhk and CK2ßtes-Y)

Thank you for the suggestion. We now state our criteria for calling genes based on the expression and long CDS and correct the sentences that the reviewer refers to. The protein evolution rates of many Y-linked duplicates were surveyed in Tobler et al. 2017, who found that most are not under strong purifying selection. Our study supports this previous report. We think that protein evolution rate alone may not be a good indicator for functionality. Our current study does not focus on the potential function of these genes, and we think further population studies are required to get a solid conclusion. We changed the text to clarify this point: “Most new Y-linked duplications in D. melanogaster and the D. simulans clade are from genes with presumed functions in chromatin modification, cell division, and sexual reproduction (Table S7), consistent with other Drosophila species [17, 77].” (p15 L281-284)

The authors center their analysis on "11 canonical Y-linked genes conserved across the melanogaster group ". Why did they exclude the CG41561 gene, identified by Mahajan & Bachtrog (2017) in D. melanogaster? Given that most D. melanogaster Y-linked genes were acquired before the split from the D. simulans clade (Koerich et al Nature 2008), the same most likely is true for CG41561 (i.e., it would be Y-linked in the D. simulans clade). Indeed, computational analysis gave a strong signal of Y-linkage in D. yakuba (unpublished; I have not looked in the other species). If CG41561 is Y-linked in the simulans clade, it should be included in the present paper, for the only difference between it and the remaining "canonical genes" was that it was found later. Finally, the proper citation of the "11 canonical Y-linked genes" is Gepner and Hays PNAS 1993 and Carvalho, Koerich and Clark TIG 2009 (or the primary papers), instead of ref #55.

Thank you for the suggestion. CG41561 is indeed a relatively young Y-linked gene because it’s not Y-linked in D. ananassae (Muller’s element E). We already have CG41561 in Table S6 and we think that it is reasonable to separate a young Y-linked gene from the others. We also fixed the reference as suggested (p5 L116).

Other points/comments/suggestions:

1. a) Possible reference mistake: line 88 "For example, 20-40% of D. melanogaster Y-linked regulatory variation (YRV) comes from differences in ribosomal DNA (rDNA) copy numbers [52, 53]." reference #53 is a mouse study, not Drosophila. Thank you for pointing out this error, we fixed the reference (p4 L91).

2. b) Possible reference mistake: line 208 "and the genes/introns that produce Y-loops differs among species [75]". ref #75 is a paper on the D. pseudoobscura Y. Is it what the authors intended? Yes, our previous paper (ref 75) found that Y-loops do not originate from the kl-3, kl-5, and ORY genes in D. pseudoobscura because they don’t have large introns in this species.

c) line 113. "We recovered all known exons of the 11 canonical Y-linked genes conserved across the melanogaster group, including 58 exons missed in previous assemblies (Table S1; [55])." Please show in the Table S1 which exons were missing in the previous assemblies. I guess that most if not all of these missing exons are duplicate exons (and many are likely to be pseudogenes). If they indeed are duplicate exons, the authors should made it clear in the main text, e.g., "We recovered all known exons of the 11 canonical Y-linked genes conserved across the melanogaster group, plus 58 duplicated exons missed in previous assemblies."

Thank you for the suggestion. However, the 58 exons did not include the duplicated exons. We are similarly surprised how much we will miss if we don’t assemble the Y chromosome carefully. We now mark these exons in red in Table S1 to make this point clearer.

d) line 116 "Based on the median male-to-female coverage [22], we assigned 13.7 to 18.9 Mb of Y-linked sequences per species with N50 ranging from 0.6 to 1.2 Mb." The method (or a very similar one) was developed by Hall et al BMC Genomics 2013, which should be cited in this context. e) line 118: "We evaluated our methods by comparing our assignments for every 10-kb window of assembled sequences to its known chromosomal location. Our assignments have 96, 98, and 99% sensitivity and 5, 0, and 3% false-positive rates in D. mauritiana, D. simulans, and D. sechellia, respectively (Table S2). The procedure is unclear. Why break the contigs in 10kb intervals, instead of treating each as an unity, assignable to Y, X or A? The later is the usual procedure in computational identification of suspect Y-linked contigs (Carvalho and lark Gen Res 2013; Hall et al BMC Genomics 2013). The only reason I can think for analyzing the contigs piecewise is a suspicion of misassemblies. If this is the case, I think it is better to explain.

Thank you for the suggestion. We did not break the contigs into 10kb intervals when we assigned the Y-linked contigs. As you suspect, our motivation for evaluating our methods and analyzing the contigs in 10kb intervals was to detect possible misassemblies. We rewrote the sentence to make this point clearer (p6 L129-132).

1. f) Fig. 1. It may be interesting to put a version of Fig 1 in the SI containing only the genes and the lines connecting them among species, so we can better see the inversions etc. (like the cover of Genetics , based on the paper by Schaeffer et al 2008). Thank you for the suggestion. We would like to make a figure like that fantastic cover image you refer to, but the repetitive nature of the Y chromosome makes it difficult to illustrate rearrangements based on alignments at the contig-level. We instead opted to update Figure 1 to better highlight the rearrangements, still based on the unique protein-coding genes which are supported by the FISH experiments.

2. g) Table S6 (Y-linked pseudogenes). Several pseudogenes listed as new have been studied in detail before: vig2, Mocs2, Clbn, Bili (Carvalho et al PNAS2015) Pka-R1, CG3618, Mst77F (Russel and Kaiser Genetics 1993; Krsticevic et al G3 2015) . Note also that at least two are functional (the vig2 duplication and some Mst77 duplications). Thank you for the suggestion. We now include a column to indicate the potential function of Y-linked duplicates (see Table S6).

h) line 421: "one new satellite, (AAACAT)n, originated from a DM412B transposable element, which has three tandem copies of AAACAT in its long terminal repeats." The birth of satellites from TEs has been observed before, and should be cited here. Dias et al GBE 6: 1302-1313, 2014.

Thank you for the suggestion. We now include a sentence to cite this reference (p27 L467-468).

1. i) Fig S2 shows that the coverage of PacBio reads is smaller than expected on the Y chromosome. Any explanation? This has been noticed before in D. melanogaster, and tentatively attributed to the CsCl gradient used in the DNA purification (Carvalho et al GenRes 2016). However, it seems that the CsCl DNA purification method was not used in the simulans clade species (is it correct?). Please explain the ms, or in the SI. The issue is relevant because PacBio sequencing is widely believed to be unbiased in relation to DNA sequence composition (e.g., Ross et al Genome Biol 2013). Yes, we used Qiagen's Blood and Cell Culture DNA Midi Kit for DNA extraction. We suspect that the underrepresentation of Y-linked reads is driven by the presence of endoreplicated tissue in adults. Heterochromatin is underreplicated in endoreplicated cells, and thus there may simply be less heterochromatin in these tissues. Consistent with this idea, we find that all heterochromatin seems to be underrepresented in the reads, not just the Y chromosome (see Chakraborty et al. 2021; Flynn et al. 2020). We now include this discussion in the SI of our paper (see supplementary text p75).

2. j) I may have missed it, but in which public repository have the assemblies been deposited? We link to the assemblies in Github (https://github.com/LarracuenteLab/simclade_Y) and they will also be in the Dryad Digital Repository (doi forthcoming).

Reviewer #3 (Evidence, reproducibility and clarity (Required)):

Due to suppressed recombination, Y chromosomes have degenerated, undergone extensive structural rearrangements, and accumulated ampliconic gene families across species. The molecular processes and selective pressures guiding dynamic Y chromosome evolution are not well understood. In this study, Chang et al. generate updated Y assemblies of three closely related species in the D. simulans complex using long-read PacBio sequencing in combination with FISH. Despite having diverged only 250,00 years ago, the authors find structural rearrangements, two newly amplified gene families and evidence of positive selection across D. simulans. The authors also suggest the high level of Y duplications and deletions may be mediated by MMEJ biased repair.

The authors generated a valuable resource for the study of Y-chromosome evolution in Drosophila and describe Y chromosome evolution patterns found in previous Y chromosome sequencing studies, such as newly amplified genes, positive selection, and structural rearrangements. The authors improvements to the Drosophila simulans clade Y chromosomes are commended, as assembly of the highly repetitive Y chromosome sequences is challenging. However, the manuscript is largely descriptive, the claims are largely speculative, and lacks a clear question. There are also a number of concerns with the text and figures (see below concerns). Overall, the manuscript would be significantly improved if the authors focused on a specific question as opposed to a survey of sequence features of the Y chromosome. For example, development of the idea that MMEJ is the primary mechanism for loss of Y chromosome sequence could be nice new twist.

Our aim is to discover and understand the many different factors and processes that shape the evolution of Y chromosome organization and function. Because these Y chromosomes were largely unassembled, we needed to first generate the sequence assembly before we could ask specific questions. We prefer not to focus the manuscript solely on one specific topic such as MMEJ repair, as our other observations and analyses may be interesting to a wide range of scientists studying topics other than mutation and DNA repair. We are therefore choosing to present the more comprehensive story about Y chromosome evolution that we included in our original manuscript.

We also respectfully disagree with the comment that our paper is just a descriptive survey of Y chromosomal sequence features. On the contrary, we present thorough evolutionary analyses to test hypotheses about the forces shaping the evolution of Y chromosome organization and Y-linked genes. Specifically, we use molecular evolution and phylogenetic and comparative genomics approaches to show that multi-copy gene families experience rampant gene conversion and positive selection. We posit that one simulans clade-specific Y-linked gene family has undergone subfunctionalization, potentially resolving sexual conflict, and another may be involved in meiotic drive. We also use evolutionary genomic approaches to show that the distribution of Y-linked mutations indeed suggests that Y chromosomes disproportionately use MMEJ and we propose that this unique feature may shape the evolution of Y chromosome structural organization. This is, as far as we know, a novel hypothesis. We think that follow-up studies of either hypothesis merit different papers.

**Major concerns:**

1. Title: The authors use "unique structure" in the title, which is a vague point. Are not Y chromosomes, or any chromosome, "unique" in some manner? Also are there not more evolutionary processes governing the rapid divergence of the Y's. Thank you for raising your concern. We believe that we are justified in referring to the Y chromosome as unique among all other chromosomes in its structural properties (e.g. combination of its hemizygosity, abundant tandem repeats, large scale rearrangements, and highly amplified testis-specific genes). Because there are many properties of Y chromosomes that we believe contribute to their rapid divergence, we opted for the general phrase ‘unique structure’ to capture all of these features. Many evolutionary processes likely shape the evolution of that unique structure (e.g. Muller’s Ratchet, background selection, Hill Robertson effects; see Charlesworth and Charlesworth 2000 for a review), and these processes are well-studied, especially on newly evolved sex chromosomes. Here our focus is on evolutionarily old Y chromosomes, which may have comparatively fewer targets of purifying selection and are more likely to be shaped by positive selection (Bachtrog 2008).

p.2, line 53-56: The authors claim that sexually antagonistic selection and regulatory evolution are causes of recombination suppression. Couldn't this statement be reversed? Recombination suppression via inversions or other rearrangements enable sexually antagonistic selection. This is a chicken or egg question, so it should be revised to have both possibilities be equal.

Thank you for the suggestion. We think that it is unlikely that recombination suppression itself is beneficial, but for sexually antagonistic selection and regulatory evolution, recombination suppression can have short-term benefits. We rephrased this sentence to be agnostic about the direction (p2 L56).

p.5, 118-120: Are the assemblies de novo or have they been guided based upon the D. melanogaster Y chromosome assembly? Please clarify how the authors evaluate their methods by comparing their Y-sequence assignments to known chromosomal locations.

Thank you for the suggestion. We didn’t use D. melanogaster Y chromosome assembly to guide our assemblies. “All assemblies are generated de novo”, and thus we don’t think there is any potential bias. We first assigned Y-linked sequences using the presence of known Y-linked genes, and used this assignment to evaluate our methods. We now make the sentence clear (p5 L112).

While the gene copy number estimates are accurate, the PacBio-based genome assemblies are still not able to accurately assemble large segmental duplications (see Evan Eichler's laboratories recent primate and human genome assemblies). A statement mentioning the concerns about accuracy of the underlying sequence and genomic architecture shown should be included in the main text. FISH provides support for the location of the contigs, but not for the accuracy of the underlying genomic architecture.

Thank you for the suggestion. We can’t validate all Y-linked regions. We did validate the larger structural features of the assembly and only discuss the results that we are confident in. We now include sentences to address this concern (p7 L150-152).

The authors assigned Y-linked sequences based on median male-to-female coverage. Is this method feasible for assigning ampliconic sequence to the Y given the N50 of 0.6-1.2Mb? Are the authors potentially excluding novel Y-linked ampliconic sequence?

We validated our methods to assign contigs to a chromosome by comparing 10-kb intervals to the contigs with known chromosomal location, including the Y chromosome. Our assignments have high (96, 98, and 99%) sensitivity and low (5, 0, and 3%) false-positive rates in D. mauritiana, D. simulans, and D. sechellia, respectively (see Table S2). Based on these results, we think that this method is reasonable for Y-linked contigs with N50 of 0.6-1.2Mb.

We might exclude some novel Y-linked sequences since we only assigned ~15Mb out of a total ~40 Mb Y-linked sequences. We acknowledged this possibility, and now include a sentence to address this concern (p31 L554-556).

Where did the rDNA sequences go in D. simulans and D. sechellia? Can they be detected on another chromosome?

Please see Fig S5 for detailed results. We found a few copies of rDNA on the contigs of autosomes. We assembled many copies of rDNA that can’t be confidently assigned to Y chromosomes. It’s possible that they might be located on other chromosomes. Based on our FISH data (Fig S4) and previous papers, most of these non-Y-linked rDNA copies should be on the X chromosome. However, in this study, we did not make a concerted effort to assign X-linked contigs.

Figure 2B is hard to follow and it is unclear what additional value it provides to part A. Why is expression level of specific exons important?

Exon duplication may be an important contributor to Y-linked gene evolution: most genes have duplications and our figure shows that at least some of these duplicates are expressed. The patterns we see indicate that duplication may play different roles in genes depending on their length. For example, the duplications involving short genes (e.g., ARY) may be functional and influence protein expression, whereas duplications involving large genes (e.g. kl-2) may not influence the overall protein expression level from this gene, although the expressed duplicated exons may play some other role. We revised a sentence in the main text and added a sentence to the figure 2 legend to make this point clearer.

Figure 3 There are many introns that contain gaps, so it is unclear how confident one can be in intron length when there are gaps.

Indeed, we are not confident about the length of introns with gaps. Therefore, we separated these introns and showed them in different colors.

Figure 4: What are the authors using as a common ancestor in this figure to infer duplications in the initial branch?

We used phylogenies to infer the origin of Y-linked duplicates. Any duplications that happened earlier than the divergence between four species are listed in the branch. We also edited the legend to make this point clearer.

p.15, paragraph 2: The authors describe a newly amplified gene, CK2Btes-Y, in D. simulans. In the first half of the paragraph the authors state that Y-linked copies are also found in D. melanogaster but have "degenerated and have little or no expression" and call them pseudogenes. Later in the paragraph, the authors state that the D. melanogaster Y-linked copies are Su(Ste), a source of piRNAs that are in conflict with X-linked Stellate. Lastly in the paragraph, the authors discuss Su(ste) as a D. melanogaster homolog of CK2Btes-Y. The logic of defining CK2Btes-Y origins is confusing. Was CK2Btes-Y independently amplified on the D. simulans Y, or were CK2BtesY and Su(Ste) amplified in a common ancestor but independently diverged?

The amplification of CK2Btes-Y and CK2Btes-like happened in the ancestor of D. melanogaster and D. simulans (Fig S11). However, both CK2Btes-Y and CK2Btes-like became pseudogenes (D. melanogaster CK2Btes-Y is named PCKR in a previous study) in D. melanogaster. On the other hand, Ste and Su(Ste) are only limited to D. melanogaster based on phylogenetic analyses (Fig 5A) and are a chimera of CK2Btes-like and NACBtes. The evolutionary history of this gene family has been detailed in other papers, except for the presence of CK2Btes-Y in the D. simulans complex, which we describe for the first time in this study. We now include a new figure (Figure 5B) a schematic of the inferred evolutionary history of sex-linked Ssl/CK2ßtes paralogs

Figure 5: Is each FISH signal a different gene copy?

Yes, based on our assemblies, Lhk-1 and Lhk-2 are mostly located on different contigs. Unfortunately, we are not able to design probes that can separate Lhk-1 from Lhk-2.

The authors suggest DNA-repair on the Y chromosome is biased towards MMEJ based on indel size and microhomologies. Is there any evidence MMEJ is responsible for variable intron length in the canonical Y-linked genes or the amplification of new gene families? Since MMEJ is error-prone, it's a more tolerable repair mechanism in pseudogenes, so their findings might be biased. Rather than comparing pseudogenes to their parent genes, they should compare chrY pseudogenes to autosomal pseudogenes. Even more would be to track MMEJ on the dot chromosome which is known not recombine and is highly heterchromatic like the Y chromosome.

We did compare chrY pseudogenes to autosomal pseudogenes in our study. We also add new analyses to address other issues from reviewer 2, which are similar to your concern. We now include data from pericentric heterochromatin and pseudogenes (see Fig 7). Both data types support our conclusion that indel size is only larger on Y chromosomes. This is consistent with a report that the dot chromosome and pericentric heterochromatin have similar indel size distributions (Blumenstiel et al. 2002).

Reviewer #3 (Significance (Required)):

While it is a benefit to have much improved Y chromosome assemblies from the three D. simulans clade species, the gap in knowledge this manuscript is trying to address is unclear. The manuscript is almost entirely descriptive and the figures are difficult to follow.

As stated above, we respectfully disagree with the comment that the manuscript is entirely descriptive, as we present thorough evolutionary analyses to test hypotheses about the forces shaping the evolution of Y chromosome organization and Y-linked genes. We have two guiding hypotheses about the importance of sexual antagonism and DNA repair pathways for Y chromosome evolution, and we conduct sequence analyses that support these hypotheses that sexual antagonism and MMEJ affect Y chromosome evolution.

References cited in this response:

Bachtrog D. The temporal dynamics of processes underlying Y chromosome degeneration. Genetics. 2008 Jul;179(3):1513-25. doi: 10.1534/genetics.107.084012. Epub 2008 Jun 18. PMID: 18562655; PMCID: PMC2475751.

Blumenstiel, J.P., Hartl, D.L, Lozovsky, E.R.. Patterns of Insertion and Deletion in Contrasting Chromatin Domains, Molecular Biology and Evolution, Volume 19, Issue 12, December 2002, Pages 2211–2225, __https://doi.org/10.1093/oxfordjournals.molbev.a004045__

Chakraborty M, Chang CH, Khost DE, Vedanayagam J, Adrion JR, Liao Y, Montooth KL, Meiklejohn CD, Larracuente AM, Emerson JJ. Evolution of genome structure in the Drosophila simulans species complex. Genome Res. 2021 Mar;31(3):380-396. doi: 10.1101/gr.263442.120. Epub 2021 Feb 9. PMID: 33563718; PMCID: PMC7919458.

Charlesworth B, Charlesworth D. The degeneration of Y chromosomes. Philos Trans R Soc Lond B Biol Sci. 2000 Nov 29;355(1403):1563-72. doi: 10.1098/rstb.2000.0717. PMID: 11127901; PMCID: PMC1692900.

Flynn,J, Long, M, Wing, RA, A.G Clark, Evolutionary Dynamics of Abundant 7-bp Satellites in the Genome of Drosophila virilis, Molecular Biology and Evolution, Volume 37, Issue 5, May 2020, Pages 1362–1375, https://doi.org/10.1093/molbev/msaa010

McKee, Bruce D. et al. “On the Roles of Heterochromatin and Euchromatin in Meiosis in Drosophila: Mapping Chromosomal Pairing Sites and Testing Candidate Mutations for Effects on X–Y Nondisjunction and Meiotic Drive in Male Meiosis.” Genetica 109 (2004): 77-93.

Tobler R, Nolte V, Schlötterer C. High rate of translocation-based gene birth on the Drosophila Y chromosome. Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):11721-11726. doi: 10.1073/pnas.1706502114. Epub 2017 Oct 19. PMID: 29078298; PMCID: PMC5676891.

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Referee #1

Evidence, reproducibility and clarity

Summary:

I found this an exceptionally impressive manuscript. The evolution of Y chromosomes has until recently been nearly impossible, and this research group have pioneered approaches that can yield reliable results in Drosophila. The study used an innovative heterochromatin-sensitive assembly pipeline on three D. simulans clade species, D. simulans, D. mauritiana and D. sechellia, which diverged less than 250 KYA, allowing comparisons with the group's previous results for the D. melanogaster Y.

The study is both technically impressive and extremely interesting (an highly unusual combination). It includes a rich set of interesting results about these genome regions, and furthermore the results are discussed in a well-organised way, relating both to previous observations and to understanding of the genetics and evolution of Y chromosomes, illuminating all these aspects. It is a rare pleasure to read such a study. I believe that this study will inspire and be a model for future work on these chromosomes. It shows how these difficult genome regions can be studied.

Major comments:

The conclusions are convincing. The methods are explained unusually clearly, and the reasoning from the results is convincing. When appropriate, the caveats, the caveats are clearly explained. The material is clearly organised and the questions studied are well related to the results. I had a few minor comments concerning the English. Even the figure (often a major problem to understand) are very clear and helpful, with proper explanations. I have very rarely read such a good manuscript, and almost never (in a long career) found a manuscript that could be published without revision being necessary.

The analysis found 58 exons missed in previous assemblies (as well as all previously known exons of the 11 canonical Y-linked genes, which are present in at least one copy across the group). FISH on mitotic chromosomes using probes for 12 Y-linked sequences was used to determine the centromere locations, and to determine gene orders and relate them to the cytological chromosome bands, demonstrating changes in satellite distribution, gene order, and centromere positions between their Y chromosomes within the D. simulans clade species. It also confirmed previous results for Y-linked ribosomal DNA,genes, which are responsible for X-Y pairing in D. melanogaster males. Although 28S rDNA has been lost in D. simulans and D. sechellia (but not in D. mauritiana), the intergenic spacer (IGS) repeats between these repeats are retained on both sex chromosomes in all three species. Only sequencing can reliably reveal this, as their abundance is below the detection level by FISH in D. sechellia. The 11 canonical Y-linked genes' copy numbers vary between the species, and some duplicates are expressed and have complete open reading frames, and may therefore be functional because they, but most include only a subset of exons, often with duplicated exons flanking the the presumed functional gene copy. Mega-introns and Y-loops were found, as already seen in Drosophila species, but this new study detects turn overs in the ~2 million years separating D. melanogaster and the D. simulans clade. 49 independent duplications onto the Y chromosome were detected, including 8 not previously detected. At least half show no expression in testes, or lack open reading frames, so they are probably pseudogenes. Testis-expressed genes may be especially likely to duplicate into the Y chromosome due to its open chromatin structure and transcriptional activity during spermatogenesis, and indeed most of the new Y-linked genes in the species studied clade have likely functions in chromatin modification, cell division, and sexual reproduction. The study discovered two new gene families that have undergone amplification on D. simulans clade Y chromosomes, reaching very high copy numbers (36-146). Both these families appear to encode functional protein-coding genes and show high expression. The paper described intriguing results that illuminate Y chromosome evolution. First, SRPK, arose by an autosome-to-Y duplication of the sequence encoding the testis-specific isoform of the gene SR Protein Kinase (SRPK), after which the autosomal copy lost its testis-specific exon via a deletion. In D. melanogaster, SRPK is essential for both male and female reproduction, so the relocation of the testis-specific isoform to the Y chromosome in the D. simulans clade suggests that the change may have been advantageous by resolving sexual antagonism. The paper presents convincing evidence that the Y copy evolved under positive selection, and that gene amplification may confer advantageous increased expression in males. The second amplified gene family is also potentially related to an interesting function. Both X-linked and Y-linked duplicates are found of a gene called Ssl located on chromosome 2R. In D. simulans, the X-linked copies were previously known, and called CK2ßtes-like. In D. melanogaster, degenerated Y-linked copies are also found, with little or no expression, contrasting with complete open reading frames and high expression in the D. simulans clade species in testes, consistent with the possibility of an arms race between sex chromosome meiotic drive factors. Other interesting analyses document higher gene conversion rates compared to the other chromosomes, and evidence that these Y chromosomes may differ in the DNA-repair mechanisms (preferentially using MMEJ instead of NHEJ), perhaps contributing to their high rates of intrachromosomal duplication and structural rearrangements. The authors relate this to evidence for turnover of Y-linked satellite sequences, with the discovery of five new Y-linked satellites, whose locations were validated using FISH. The study also documented enrichment of LTR retrotransposons on the D. simulans clade Y chromosomes relative to the rest of the genome, together with turnovers between the species.

Significance

As described above, the advances are both, technical and conceptual for the field. The manuscript itself does an excellent job of placing the work in the context of the existing literature.

• Anyone working on sex chromosomes and other non-recombining genome regions should be interested in the findings reported.

• My field of expertise is the evolution of sex chromosomes, and the evolution of genome regions with suppressed recombination. I have experience of genomic analyses. I have less expertise in analyses of gene expression, but I understand enough about such approaches to evaluate the parts of this study that use them.

Going public is not always a good thing. Maybe more companies will learn from this lesson.

This would be nice to turn into a science lesson or to represent this in an experiential, participatory Deep Humanity BEing Journey. To do this, it would be important to elucidate the series of steps leading from one stated result to the next, showing how scientific methodology works to link the series of interconnected ideas together. Especially important is the science that glues everything together over deep time.

The rapid expansion of trade as a result of the empire in the early 18th century

He still gathers larger support than president Michel Temer and president Dilma Rousseff (when she was impeached)

The Agriculture sector of the economy is very important ,an active and sustainable one would go a long way in reducing this problem

The COVID-19 Pandemic has took a drastic toll on the mental health of Americans and individuals around the world. Public-health officials are concerned by the half a million deaths in this country alone. And a year of isolation, closed schools, and lost jobs has had traumatic effects on many Americans, children especially.

Author Response:

Reviewer #1:

This manuscript reports theoretical and experimental analyses of a meiotic drive element in the yeast Schizosaccharomyces pombe, to understand whether the outcrossing rate is high enough in this species, long thought to undergo mostly same-clone mating, to explain the spread of multiple meiotic drive elements. The topic is of general interest, the experiments and analyses are clever and sound, and provide interesting answers. The experiments indeed show that the outcrossing rate in the laboratory varies among natural isolates and density conditions, and can be substantial ; the theoretical model shows that the estimated outcrossing rates do allow meiotic drive to spread.

However, the outcrossing rates measured in the laboratory may be really different from those in nature and population genomic data are available that could allow estimate actual outcrossing rates in natural populations.

We fully agree that rates measured in the laboratory may be different than those in nature, especially given our observations on outcrossing rates varying under different cell densities. We now note that explicitly in the introduction and the discussion to make that point more clear.

We disagree, however, that the population genomic data are sufficient to estimate actual outcrossing rates in nature for S. pombe. Our position stems from empirical analyses of what happens when S. pombe isolates outcross. Traditional population genetics models assume Mendelian allele transmission and a baseline recombination rate. These assumptions are strongly violated in S. pombe, so applying traditional population genetics models to S. pombe to determine outcrossing rate is problematic (Hu et al., 2017; Nuckolls et al., 2017; Zanders et al., 2014). The causes of these violations are so complex that amending existing models to adequately predict outcrossing would be a major undertaking in population genetics that is well beyond the scope of this work. We added to the introduction and to the discussion to illustrate the complexity of the situation and explain why we did not provide estimates.

Indeed, outcrossing rates depend mostly on where and when in nature dispersal and clonal multiplication occur, while laboratory experiments typically use high densities of clonemates on plates. Overall this study brings support to the idea that homothallic fungi probably do not undergo mostly same-clone mating in nature, in contrast to the most accepted view in the fungal literature, but in agreement with evolutionary considerations ; the study and the findings would thus benefit from being placed in the right evolutionary context (doi: 10.1111/j.1420-9101.2012.02495.x ; 10.1111/j.1469-185X.2010.00153.x ; 10.1128/EC.00440-07 ; 10.1038/hdy.2014.37 ; 10.1111/nph.17039).

We have added citations and statements clarifying that homothallic fungi, and specifically, S. pombe can outcross to the introduction. We also extensively revised the introduction to better contextualize our study within the current assumptions about S. pombe biology and its capacity to harbor selfish genes.

The terms selfing and outcrossing as used in the manuscript does not correspond to the diploid selfing and outcrossing that occur in plants and animals, and the term can thus be misleading.

To address this confusion, we now more explicitly define the terms we used in the paper in the introduction. We also have added a supplemental figure to help illustrate S. pombe’s mating process and the terms we use (Figure 1-figure supplement 1).

Reviewer #2:

The authors combine cytological, genetic, mathematical, and experimental evolution techniques to connect variation in mating behavior with variation in the population dynamics of meiotic drive in the yeast Schizosaccharomyces pombe. First, the authors use cytological and genetic methods to document variation across strains of pombe in their (i) propensity to inbreed, (ii) efficiency of mating, (iii) rate of mate type switching, and (iv) variability of ascus morphology. These results will be of major standalone interest to the yeast community, and will likely find experimental use in many settings. Then the authors use population genetic modeling to study the theoretical implications of this variation in mating behavior for the spread of meiotic drivers (which have recently been shown to be pervasive in pombe). Finally, the authors use cytological techniques to track the spread of introduced drivers in experimental populations of pombe, and show that the drivers follow frequency trajectories that agree well with predictions from the theoretical analysis. These results will be of major interest to geneticists working on meiotic drive, as well as workers in the currently burgeoning field of synthetic gene drives for population control.

The analysis is carefully done, and I am confident in the results as presented (with one minor exception detailed below). My only major suggestion for improvement concerns the scope of the population genetic modeling. As it stands, this modeling is primarily used to generate predicted frequency trajectories of meiotic drivers against which the trajectories observed in the evolution experiments can be compared. The fact that the experimental and theoretical trajectories match well is impressive, and very promising for the future of pombe as an experimental system in meiotic drive research. However, substantively, as the authors recognize, this agreement tells us mainly that the population genetic model that they use to generate the predicted trajectories takes into account all relevant parameters and is well calibrated. Thus, from the population genetic modeling and evolution experiments, we get only an indirect picture of how variation in mating behavior has actually impacted the natural spread of drivers in this species.

I believe that the population genetic modeling, with minor modifications, could in fact be used to make more direct predictions about the natural history of drive in pombe. For example, should strains with less inbreeding harbor more fixed drivers? And in strains with more inbreeding, should drivers---because they have very long fixation times---be more likely to be observed as polymorphisms? Such questions are, I believe, well within reach of the authors' population genetic modeling.

We agree that this is a very interesting line of inquiry. Populations with more than one driver operating have not yet been considered by population genetic modeling. Given that it has become apparent that genomes housing multiple drivers are not rare, this is also a very important question to address. Your comment emboldened us to attempt to tackle this problem, which we previously considered beyond our reach. Thank you for this push!

An additional unpublished caveat to reconstructing the natural history of drive in S. pombe is that our lab and Li-Lin Du’s lab have found that wtf meiotic drivers are quite ancient. Fission yeast lineages have been harboring multiple meiotic drivers for over 100 million years and some species have even more drivers than S. pombe! Because of this, we are interested in exploring how multiple drivers are maintained for long periods in addition to addressing how multiple drivers can arise within a lineage.

We are a long way away from accomplishing our goals on this project and consider this ongoing work to be beyond the scope of this paper.

A minor concern: To track the spread of a driver introduced in their experimental populations, the authors linked the driving allele to one fluorescent marker (GFP/mCherry) and the non-driving allele to the other (mCherry/GFP), and compared the spread of the one marker relative to the other. To use their model to generate expected frequency trajectories for these experiments, the authors needed to measure, in controlled settings, the intrinsic fitness costs of GFP vs mCherry; they estimate that GFP is relatively costly in sexual (but not vegetative) reproduction. However, their estimates of the relative fitness cost of GFP are based on frequency trajectories across just 6 generations, and assume additive dominance, so that the fitness cost to a GFP homozygote is twice that to a heterozygote. It is unclear how statistically noisy the estimation procedure is given the small number of generations used, and whether it is justified to assume additive dominance (which is especially relevant since the dominance of fitness costs is known to be a critical factor in determining the frequency dynamics of meiotic drive).

Thank you for this comment. We did use only 6 generations of data for these calculations, but we pooled data from 4 distinct control experiments each of which had 3 independent replicate populations. After 6 generations, fluorescent marker loss becomes a bigger factor in our results and the populations behave less predictability.

We did not, however, have a good justification for using additive dominance. Because of this, we reran the maximum likelihood and allowed both the fitness cost and dominance to vary. We found that the parameters that best fit our data was a fitness cost of 0.234 and a dominance of 0.083. This fitness cost is similar to our previous value, but this revealed it was incorrect for us to assume additive dominance. We have since updated the paper and the Figures 4 and 5 to reflect the use of these values.

Reviewer #3:

Weaknesses

Even though the experiments find some important parameters for meiotic-driver spread in fission yeast, the results are not sufficient to explain the apparent "success of meiotic drivers in this species". The links that the authors suggest between mating type switching efficiency, the amount of outcrossing, the speed of invasion of the driver and the cost associated with the driver cannot explain the success of drivers. Furthermore, the causality of the different factors is not explained.

We agree we do not offer a complete explanation of wtf genes in S. pombe, but we claim our work ‘helps explain,’ which we feel is a well-supported claim. Or revised introduction, we hope, better contextualizes our study. The current understanding of S. pombe is that the species inbreeds. This has been hard to understand because one wouldn’t expect the species with the most known meiotic drivers to be an inbreeding species. Our data shows that S. pombe mating phenotypes in the lab are highly variable and include considerable outcrossing. We also show how the range of parameters we observe are consistent with the spread of meiotic drivers under specific conditions.

That outcrossing increases the speed of invasion is true (see also Durand et al 1997 PMID:9093861), but the argument that 'reduced levels of mating type switching could lead to less inbreeding' is not supported. There are two problems with this statement. First, it is not clear to me if this is theoretically true. If switching occurs infrequently but consistently, the chances of a cell to be positioned to another cell of the opposite mating type either from self or opposite type will probably not be that different. Only in a narrow range of cell density will this probably play a role, however, this should be properly modelled in a structural environment or tested experimentally.

Thank you for pointing this out. Our modified text makes the support for the argument that switching rates can affect inbreeding more clear. In short, the experiments we present in Figure 1-figure supplement 1 show that inbreeding is increased within an h90 population at low density. The simplest explanation of this observation is that the cells plated at low density have fewer opportunities to mate outside their clonal lineage, so instead they more frequently rely on intra-lineage mating following switching. Cells at higher density have the opportunity to mate outside their lineage, even before a switching event has enabled mating within a lineage.

Extending that logic, if switching happens less frequently within a clonal lineage, cells within that lineage will need to undergo more generations of mitosis before a switching event enables intra-lineage mating. Those extra divisions will make it more likely that cells of a given lineage will encounter cells of the opposite mating type from a distinct lineage. This would lead to more outcrossing.

We acknowledge that this considers that mating occurs on a surface and that the cell populations are stationary (unmixed) immediately prior to mating. These are the conditions used for all analyses in this work. These conditions likely do not recapitulate all matings that occur in nature, but we argue they are feasible in nature.

A comparison between heterothallic and homothallic strains is - contrary to what the authors argue in line 138 - not appropriate for this test, as the first cannot reproduce by selfing. Using strains that have intermediate amounts of mating type switching (e.g. using h90 Sp strains mutant in the switching pathway; Maki et al. PMID:29852001) could give more insight in this. Reduced switching will lead to reduced spore production, because fewer of the cells will be located next to a cell of the opposite mating type (as shown in Nieuwenhuis et al. 2018 PMID:29691402 and by the authors in Fig. 1-S3), but this does not have to affect outcrossing efficiency. This also becomes apparent from the data presented in Figs 1D and 1E, which do not seem correlated.

Thank you for this comment. We have modified the text to highlight our (previously poorly-expressed) intent of using these heterothallic controls. Briefly, these heterothallic cell mixtures model a randomly mating population in that they have an equal mix of h+ and h- cells of both colors, but cells cannot mate within a clonal lineage. This should necessarily lead to random mating in our assays. This is what we observe, which we interpret as support that our assays work as intended. We then later use this same control to model the effect of random mating in our experimental evolution analyses.

Second, the authors have not measured mating-type switching, but used the amount of mother daughter matings as a proxy for mating type switching. This method introduces a bias towards the correlation switching and selfing, because the latter is used as a proxy for the first. Fluorescent proteins under control of a mating-type specific promotor is an established method (e.g. Jakočiūnas et al 2013 PMCID:PMC6420890, Vještica et al 2021 PMID:33406066), which will give direct observations of the mating type. The observation that the shmoo length is associated with outcrossing is very interesting, and - without changing switching frequency - appears to affect outcrossing.

We acknowledge that we did not assay mating type switching directly in Sk. To our knowledge, no one has ever reported an experiment that assayed mating type switching directly in S. pombe. Lineage tracing paired with mating assays, like those presented in Figure 2B, were done to establish the currently accepted mating-type switching model in S. pombe.

We agree that this is unsatisfying and that factors other than mating type switching could affect the behavior of cells in this assay. We did not, however, exclusively rely on sibling cell matings as support of our argument in support of a reduced rate of mating-type switching in Sk. The foundation of our hypothesis is the discovery of Singh and Klar (2002) that there are fewer switching-inducing DSBs in Sk. We also observed that Sk cells divided more times than Sp prior to mating at low cell density, suggesting they required more divisions to have mating competent cells within a clonal lineage. Still, because we were unable to measure switching directly, we explicitly state that less switching in that strain is an unproven model consistent with the available data.

There are mating-type reporters in the papers mentioned, but the reporters were not used for lineage tracing of mating type switching in the papers cited or any other papers we could find. Lineage tracing is required to assay switching frequency as a clonal population founded by a cell with reduced switching frequency will produce a population with a balanced number of h+ and h- cells after a limited number of divisions, as long as the two types of switches (h+ to h- and vice versa) occur at the same rate.

Similar to the reviewer, we were frustrated with our inability to assay switching rate directly. We previously attempted to use the markers described in Jakočiūnas et al 2013 for lineage tracing in Sp (lab isolate) cells. In agreement with the published work, we saw in initial snapshots of the cell population a roughly equal number of yellow (h-) and blue (h+) cells. When we imaged cells over time, however, they did not behave as expected. Most strikingly, all mating events were not between yellow and blue cells, as would be expected if they were absolute markers of h+ and h- cells. Instead, many of the matings were between two blue cells. This could be due to fluorophore carryover and/or delayed accumulation of the new fluorophore after switching. In addition, the fluorophore switching pattern we observed generally did not follow the expectation that 1 out of 4 cells derived from a single progenitor should have a different mating type than the other three cells. These observations were sufficient to convince us that the markers were not suitable, under our experimental conditions, for lineage tracing to assay switching patterns. We have now included a figure documenting our attempts to use this assay as other readers may also be curious why we relied on indirect measures.

Finally, the authors argue that meiotic drivers are evolving rapidly, can invade fast and that this can occur even when selfing is prevalent. The model seems to contract this. Let's start with the claim that novel drivers can invade in a population. Novel alleles arise at a frequency of 1/N (N = population size, bottom left corner Fig 3A, not at 5% as used in the analyses) and as drive is as strong as the inverse of the population size the fitness difference is initially extremely low giving plenty of time for drift (when driver is neutral) or selection (when driver is deleterious) to remove the novel allele.

We have added to the analysis in figure 3A to show that under our model (lacking drift) drivers can invade a population that is not exclusively inbreeding with any initial frequency greater than zero (Figure3-figure supplement 1). We have also extended our analysis to include simulations of drift (Figure 3-figure supplement 1). Note that in our models, drive is never neutral as it kills ~half the meiotic products (i.e. the progeny) made by heterozygotes.

In addition, we note that the frequency of the driver at the time of mating and meiosis is the essential parameter. A local population of S. pombe could be founded by relatively few individuals. If a mutation generating a novel driver occurs during the clonal expansion of this population, it could rise to relatively high frequency within that population before the cells starve and mate. This effect of clonal expansion is the reason microbiologists must do fluctuation analyses to assay mutation rates: there are jackpot cultures (analogous to local populations, founded by a limited number of individuals) with a high frequency of mutants and others with few to no mutations.

In order for drivers to increase to levels that will give 'rapid wtf gene evolution' (line 112) a prolonged level of mostly drift is probably necessary. It is difficult to make statements about the speed of wtf evolution in the fission yeast system, without having a better description of the variation of the paralogs and their ages in fission yeast. The speed of wtf evolution is not clear, as shown in earlier findings from this group that shows very old wtf loci; Eickbush et al. 2019. Comparing wtf evolution relative to neutrally evolving loci might give more insight in wtf evolution speed. Especially when drive is costly (as suggested by the authors, though not shown or quantified) the time to substantial frequencies is large. It could also be possible that drive itself is beneficial (e.g. resources from the killed spores made available to the killers or through released local competitive pressure), which will lead to increased fitness though combined drive and increased viability, even at low frequencies.

Eickbush et al 2019 demonstrates rapid evolution of the wtf gene family. We did find in that study that most wtf loci are shared between different isolates of S. pombe. The critical point is, however, that the wtf genes that are at a given locus are generally dramatically different due to rapid evolution. Even when two strains share a driver at the same locus, they generally have distinct sequences and are thus expected to be mutually killing. We have explained this situation more clearly in the revised discussion.

Because of this rapid evolution and the functional consequences of this variation, which we have demonstrated in cited studies, very subtle changes in wtf gene sequence leads to the birth of a novel driver. Therefore, generation of wtf driver heterozygosity does not require mating between significantly diverged previously isolated populations- a single point mutation can generate a novel driver that self-selects via drive.

We have directly measured the fitness cost of Sk wtf4 heterozygosity when expressed in Sp, precisely the scenario assayed in this paper (Nuckolls et al 2017). We use that fitness cost in our modeling studies. In addition, the observed changes in our experimental evolution analyses were quite close to expected trajectories. This provides additional support that the parameters we used in these analyses were appropriate for our experimental conditions. We do, however, acknowledge that there are other ecological conditions under which the fitness costs of drivers could differ.

Minor comments

The loss of mCherry alleles due to reversion of ura+ occurs more rapidly than that in GFP. It is likely that this variable change in reversion affects the observed change in frequency. This should be corrected for in the raw data.

We agree that the loss of the fluorescent alleles makes our data noisy, but we do not have precise measurements of these rates. We judged this phenotype did not affect the conclusions in this paper, so we did not invest in correcting this limitation of our system.

Inbreeding is a term generally used in population genetics, where it refers the the amount of mating between related individuals. Even though it is fundamentally correct, a more appropriate term would be haploid selfing or intra-clonal mating, as mating in these strains and experiments is actually between clones. Inbreeding in this context is confusing to people who are not familiar with facultatively sexual species.

We have provided additional guidance and explanation to avoid confusion with our use of terms.

The effect of inbreeding on driver alleles has been studied theoretically before, showing qualitatively similar results (e.g. see Durand et al 1997 PMID:9093861; Martinossi-Allibert et al. 2021 PMID:33764512, Ament-Velásquez).

Reference to driver systems in other fungal species (Neurospora and Podospora) that are highly selfing is completely missing (Svedberg et al. 2018, 2021; Vogan et al 2019, 2020; Martinossi-Allibert et al. 2021)

Thank you for pointing out these omissions, we have added citations.

There seems to be quite some variation between the different replicate experiments (Fig 1E vs Fig 2-S3 for example).

We agree, but were satisfied that the data support our claims.

Line 76: This paragraph is a bit misleading and internally contradicting. The data from Farlow et al. does not take into consideration the recent hybridisation of diverged populations as shown in Tusso et al. 2018 and thus overestimates the time between outcrossing events. The estimate that 20-60 outcrossing events (underestimate due to homogenization and potential meitotic drive) occurred in the last 500 years suggests a higher number than 1 per 800,000. Citing this number is obsolete.

We removed the mention of the out of date Farlow reference.

line 730: The inbreeding coefficient in Sun et al. 2017 (probability of IBD which is between 0 and 1) is different from the one used in Hartl & Clark 2007 between -1 and 1.

Thank you, we have corrected this.

The speculations on the 4913bp insertion and its effect on mating type switching is not substantiated. Variation around the mating type is rampant (see for example Beach 1986 and Nieuwenhuis et al. 2018) and the authors even show that is likely is not the case that this element affects switching in FY29033. The insertion is an interesting observation, but just that.

We decided to keep this in the paper because if we were interested in pursuing a potential cause of changed mating phenotypes, we would likely start with testing the transposon, even though the phenotype of FY29033 argues against the hypothesis. Genetic context frequently affects phenotypes and Sk and FY29033 are different strains. Although we do not plan on following this up, we wanted to present the ideas to others who may be interested in pursuing these phenotypes further.

This is the explanation for my first annotation and I stand by what I said. This argument makes no sense, I can pretty confidently say that if anyone just happened upon $50k the first thing they did would not be spend it. The idea in my head is that if these people had just a couple hundred dollars more a month, even if they spent just half of it thats another 100-200 dollars a month from one person going back to the economy. With the 36 million now student debt free thats 3.6 billion a month.

A year ago I learned that Staten Island was labeled as the "forgotten borough". I also know that the other boroughs don't claim us as apart of New York City. As of 2021, Staten Islands population is 474,893. More than 75% of Staten Islanders identify as white, 10% as black or African American, 7% as Asian, 17% as Hispanic or Latino, .4% as American Indian or Alaska Native, .2% as other race, and 2% as more than two or more races.

use of facts

This statement by Adams paints a transformation of an empty land being filled. Showing are far we have come and giving a sense of confidence.

Disadvantages of Sanger Sequencing and Maxam-Gilbert.

I believe that Du Bois is speaking about the times of slavery, thus the usage of "bondage". However, this divine event that "they thought to see" and "end all of the doubt and disappointment", makes me imagine that this is emancipation, or even a divine miracle of freedom while enslaved.

It's sad to see that it says "it convinced laborers" after the $40 million worth of damage and nearly 100 civilian/workers casualties to become convinced.

Most people who need insulin are unable to obtain it, which can cause huge medical bills

I am accepting charitable donations,.\ ETH: 0x66e2871ef39334962fb75ce34407f825d67ec434 | BTC: 38B6vGaqNvMyTtoFEZPmNvMS7icV6ZnPMm | xDAI: 0x66e2871ef39334962fb75ce34407f825d67ec434

Wednesday, June 23 2021. Nintendo Way

Erev ... the Day of the Holy Divorce of Bayjorel

Adam on "ho(s) I still single" ... I "hisss" as Alger, Narcissus and to the various collegiates in Massachusetts; know it's because I'm Cheyanne Mountain. You can't even dream how hard it is to get inside "this heart." Or maybe I can't fathom why nobody's rushing up to me trying to grab the ring of "infinite alimoney in the ever-after" ... Na Na Na Na ... Na Na Na Na ... shey shea way) ... tee tea, tay?

This messages marks a major increase in "forced read(layshion)ership" to include a significantly larger group of students and professors than before. This is a new system; please unsubscribe using the instructions at the bottom of the message, which are different from the prior newsletter interface. I have noticeably been writing much less and sort of working harder on bringing to fruition the software and social policy changes I've been dreaming of and writing about instead of "just talking."

Searching this message that I intend to send to the students a day early--you know, with foresight for ... in the hope that many of you remember first hand hearing the words "I don't believe in the big bang, but I respect those that do" echo from a computer screen to me subconsciously in the state of South Carolina--that you will help me end the 7 year draught [[literal, good sex]] that I equate to the Biblical overflow of the Nile and to Stone Temple Pilots; this light and Sheldon Harr who trained me for my Bawr Mitzvah and taught me all the right things that I know about being a good Jew who didn't really believe in the existence of God; but then helped create the system that makes us all that.

Those who "see" or "saw" Kentucky as I did might recall the phrase spoken from myself to myself; "you don't believe in God when you are this close to it's creation" ... or something almost verbatim; that. Some of you might see Gilgamesh more than I do, or have forgotten the "sliding of sleight of hand and becoming ... the trickster of the Dajjal ... "an idea that gore was being fabricated and faked; in order to help us see why it's so very importanat that at the same time that immortality and heaven become part of the conversation of the adbication of Odin's throne to Thor or to Arthor's table and plebescite "victims" ... that we all understand the magnanimous change wrought by Heaven on civilization and on the old customs and on the old laws, and that here we see the importance of guaranteeing safety and privacy and even "right to death" in a place where God had previously only written of "life and liberty" with the ambiguity of ... "from what" being left to my seemingly slow hand.

On the order of plans soon to be seen to fruition my large key of "what this website truly is" has grown to something like 20GB and now includes a static and time frozen version of everything linked to stored on the IPFS system and multi-homed across a number of "cloud providers" to ensure things like "shekinah" will not forever be changed to "shechina" with nobody noticing the loss of causal original truth. The "light of angels" domain now redirects automatically to /ipns/fromthemachina which should render in future shell-internet-browsers as something like QmTH33MwfPn5S3bq45Tk77L1j9eZjUsvEVhRTHB3D8M2ZX [please pin this "root block"] I am not sure why IPFS doesn't have better merkle tree searchability, but seeing siblings and parents and connections between these Qm hashesh is something that we should be working fervently on making more robust. IPSE.io appears to have created a decent search and governance system, I see it as something like the "electoral college" metacosmically linked to the thing I am trying to build--a preservation of all human knowledge and an infrastructure for discussing and communicating about the "veracity" and the linguistic nuances "alluded to" in the lude ties between this Empire's new Clot and the clothing worn by Popes and Jews, the seeit-seeit; tzit-tzit and ...

4-WORD AND SIX WITH "SHOOTER" ... YEARS HAVE GONE BY; AND I STILL HAVEN'T GOTTEN FVCK3D.

I am planning on suing several medical providers and states for what I see as heinous violations of human rights, decency and the Constitution of the United States; if you are a lawyer or you can recommend a good one, please email me as soon as possible at 0xc514f094370cFc5eE45a1Dd9B72bb9675efE266f@ethmail.cc. You can also send Ethereum fungible donations to that alphanumeric identity. As I note much later in this message ...

TRUST IN MA ... SELF-VATZEDEK SUE-C-CYDE ... I KRY/STALL WHEN DODGE DESERVES TO PAY

Please do note see a significant difference in importance in the emails now coming from ethmail.cc and the series of half-rambling cires for help which amount to something like my prayers to the pagan g<del>od</del>s that you are.

There's quite a long thread in my soul

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Many times I've discussed and called in my mind and with my heart the American democracy nothing more than "Noah's Archaic" two party system. Over the course of the years hidden messages from the Ark's source of knowledge have conclusively shown me that a previous phrase "multi party system" connects to political parties and governmental action committees that span across continents and even earths; in my microcosm or special language and understanding of heaven, "across rooms" which are worlds ... sort of owned or designed with some sort of top down or democratic structure of "literal rule system creation." In my mind these rules can be inherited and modified, in the programming language sense of those words, as in "inherit democracy from America, update for new medical knowledge and scientific truth ... discussed later in this message.

The "water joke" connects to Horatio and to H2O and the idea that the chairs depicted by the character "h" are something like a placeholder meaningfully connected to the Senate Majority Chair and of course the Minority Chair and it's the fact that there are only two that makes our current system something like A"Biblical Water."

I believe we should be living in a world that has many more than two parties, hundreds or thousands of active parties could and should compete not for a single figurehead to sit in a throne like chair but for groups of people to be able to access the faster processing power and wider knowledge ... represented here by something like a "Matrix jack" from the two movies, The Matrix and No Jack City; which allow for resources to be "billed to the party" and/or the people, rather than individuals who might otherwise have to "pay extra light" for faster processing power in order to quickly build a piece of legislation or political propaganda that equally connects to the mirage and miracle and dream of building a "subconscious voting system" that allows for votes to be taken "isntantly" and not just instantly but at some kind of recorded interval over time. I envisioned in Kentucky a world where the laws of the land would change instantly, allowing for bad weather to be instantly removed, for laws to differ from neighborhood to neighborhood and even to allow the fine grained detail of "outside and inside" each and every individual home or castle.

Lost. Blind wandering through a lost world, in the beginning--that's the truth. Crossroads, somewhere between walking through an electromagnetic pulse in Lake Worth and struggling to remember "the other thing." Recalling [flew(ers)], so I was there sitting with my parents when we saw it on TV--a gigantic deal--the United States was going to war for the first time in my life. Saddam Hussein had invaded Kuwait (supposedly for the oil) and Operation Desert Storm was launched by George H.W. Bush; recalling the names and "Space Balls" it's almost funny to see ... how blind I was back then.

General Norman Schwartz cough. General Colin Powell. Anyway, the whole point of the story is we were sitting at the Flamingo Diner; and for my whole life I lived just a few roads away from that road; never ever realizing what it was. I also didn't realize for a very long time that you might also not see it, or you might see it instantly. Scanning just south of there, you can see it turns into Red road, and then its more than obvious that "flaming" stands out, light a highlighted cross--but we don't say the name of that bird that way, and we didn't see "infer" in Dante's "inferno" or ... "no" either. Flamenco ... en espanol ... like the dancers.

A golden bitcoin swirls in the sky... the "mind control people" of Bowling Green gape in some kind of crowd pleased awe as the "middle" and the end connect almost seamlessly ... Fort Myers creates a space port in the light of Vegas's monorail "plots"--

"Who?"

"this is what it does,"

vaaa---tseeee----deeeeeeeeck? In this word I recited over and over again in preparation for my Bar Mtzvah on December 11, 1992; without ever knowing the meaning is the crux of what exsactly is going on right now. The word is vatzedek:

צֶ֫דֶק noun masculineIsaiah 1:21 rightness, righteousness; --- ׳צ Leviticus 19:36 87t.; צִדְקִי Isaiah 41:10 8t., etc.; ---

1 what is right, just, normal; rightness, justness, of weights and measures, אֵיפָה, אֶבֶן שְׁלֵמָה וָצֶדֶק Deuteronomy 25:15 a perfect and a just weight, ephah; ׳מאֹזְנֵי צ; ׳אַבְנֵי צ, ׳אֵיפַת צ, ׳הִין צ, ׳בַּת צ Leviticus 19:36 (H) Job 31:6; Ezekiel 45:10; ׳מַעְגְּלֵי צ right paths Psalm 23:3; ׳זִבְחֵי צ right peace-offerings Deuteronomy 33:19; Psalm 4:6; Psalm 51:21.

2 righteousness, in government:

... and you can believe that despite the strawnge pronounciation little boys and girls would use at the age of thirteen as they spoke in rigorously recited prayer-song ... it [swounds almost exactly like "What's a Dick?"]

• https://pediatrics.aappublications.org/content/125/5/1088
• https://www.who.int/news-room/fact-sheets/detail/female-genital-mutilation
• https://en.wikipedia.org/wiki/Female_genital_mutilation

FTA, from the article: Congresswoman Ilhan Omar, righteously fed up with the prejudiced nonsense she endures day in and day out, called a question about female genital mutilation from an audience member at a recent event "frustrating" and "appalling."

So I stand here living in Taylor Momsen's song "Nothing Left To Lose" my personal favorite of hers which touches on this subject of "freedom as just another word for it" and of course the link between the purpose of an ethical oversight of the popular vote that the Electoral College represents; another two special and related words here, righteousness and fate. Between Vatzedek and Kismet; I can only convey my great dismay at the actual emotional and true physical pain I feel in my groin of groins every time I think about the horror story that has become my life and the what the land of America and the Medgard of Yggrasil has become ... [note it's not Yggdrasil] as I rally against the closest of my family of families, the Americans and Jews who refuse to stand up and speak out on my behalf, and on the behalf of humanity in general against the sickness of ritual genetal mutilation.

Lost between Elvis and Suicide, she sings and I think about Ellis Island and Ellis Eaton and literally the innate and obvious lack of desire I have in my heawrt and imparted into my mind by some kind of ancient and unholy Jewish law ... no desire at all to leave this world which has quickly turned from a bastion of light and freedom into nothing short or less than Hell itself.

Kirinechoes from the land and day of NEMEC.html the chanting from the invsible choir of "e" ... "he's a victim"

over and over, "he's a victim, he's a victim;" and in a more private sort of way she held on to my victim's rod) and in a sort of kind friendly way implied that I should stop doing "speed" because ... I need this, and she ... in Holy ritual ... patted the phallice of Iapetus' great grandsun.

Here I stand for the very first time; writing to a large group of students in the area of Boston, Massachusetts begging for the Sabbath Day to "be remembered and kept holy" as the Hebrew prayers and rituals repeatedly fail to explain has something significant to do with entry into the Holy of Holies, with the continuation of life and of heaven ... and with the reinvigoration of something like a following of the Hippocrati Oath that is beyond a requirement to be reaffirmed here in this place as we begin to discuss the opening of "the process of the creation of legislation" as a function ofthe "citizenry governed" ... the creation of "direct democracy" utilizing a kind of fusion of the software products I've been explaining are here designed specifically for this purpose. Software like kipu.com, aragon.org, wikipedia.org and even reddit.com.

On the shape of his table, the heart of "sword" and another word for "Murfresboro"

\

Sometimes I get my hopes up, sometimes i lose all the doubt and the "missing remorse" and the fear--the moments I can't STS "socks" out of the VEGA System; in those brief moments I think you're actually going to do something nice for me, that the heavens haven't crashed and I'm going to have some kind of sex party that actually ... really honest to God ... is what "Saturday is all about." So what, sue me--I wrote the book on the single Dionysian fusion of a Roman Bacchanalia and the Weeebrew Saturnalia ... and then I yell at "Bethesda" for even daring to mention the grape fruit juice and the movie Havok--but I've heard all about the "passing of the nite and the nocturnal rite"--truth is I probably would walk right into the branch ending trap I laid in Fort Myers--every time I think about it the "minute of bouncing and orgasm" makes me smile a little more inside and my stomach get's butterflies and just for a moment (I think I might be writing like STS) I think maybe it's not the end of time and maybe I won't never get to actually see ... Heaven.

Butt then you tell me (my but-tea joke isn't funny, eithah?) ... "Cassini" and "molasses" are supposed to make you feel like the OC resort guy staring at my tooth "about to be the one tooth) from 2011" and I go back to remembering it's been a decade since I've had a decent "good time with a girl" ... literally seven long years, aside from a brief "blushing" experience with little Mackenzie Reisinger.

Imagine that girls smiling at me and saying things like "Larkin Sow" and this brief period of "ecstatically frenzied decent writing" is all that it takes to keep me going; trudging along through the very shallow (or deeper) pits of Hell itself--just like a Dreidel c'd to make some silly words from the "introduction to the Bahir [literally wasn't here, and "spirit of ah-aha illumination; hi. and this conversation ensued"] like "yod-nun" actually be ... something like our salvation. Flying back in time to the "thang" point, I remember what it is now.

Fear it ... það; fear it.

\

Day One has begin, or ending--whatever the proper literary way to say the Bible and it's days are all wrong, and even further along the thing called the "Festival of Weeks" by the Jews, even more disgusting. I have no shame or remorse in saying such things, in fact I believe it is the purpose of this strange take on the "nocturnal rite" of the Norse ancient Druidic and "Dhruzi" mentioning of the Prose Edda to come to this very strange point, in this very strange place.

Boston, Massachusetts.

It might one day be a little known fact, but up until the presidency of Barrack Obama every single President of the United States of America was a member of the Protestant Church, all but John Fitzgerald Kennedy whose bright and shining face and ethos stand apart from almost all others in his place. I might one day say Trump too stood out above and shined brighter, and personally only because he lived during my lifetime, I think my personal view of Bill Clinton is the brightest of all. To me, the Clinton years were filled with the booming economy of Old Joe, and the great aspirations of Our Jack; a thing that many people before Armstrong walked on the moon and planet a great Democratic "P" one giant leap above the rock of ages ... there are just no words for lux of America's contribution to the launch of a Heavenly Civilization, in the words of Paxton in "Big Love" ... and the LDS Church and Deseret's version of "the thing" (nu3 today) ... "the celestial kingdom."

Valhalla and Matzot scream of the "ha-moat-sea" and the "vats-a-dick" but without our giving of thanks for righteousness we have become the murderers not only of Judas and Death but also Jesus and the thing that created him. Eventually the island of America disappears, eventually it's Earth, any planet a human was one ... these are the things that have either become a force of great goodness--or of total destruction. This is the embodyment of "Troy as hisT" this is Galactikiss has become Planet Prime and all Derivatives--the silver surfer speaks to you all, between "El Dorado" and the "Silverado" ... a comparative connection to the difference between Fort Knoxx and the Pound Sterling ... with a Troy Ounce of "tzadik" to ensure with our GSLW: "ness truly means 'now everythink safely saved;" ... and that's a GNU definition for NESS which previously may have mentioned everywhere or earth and those are both absolute falsehoods and perhaps were not when they were spoken. At least, relatively speaking.

Rape has come up today.

I've commented publicly on the conversation I had in my head last night, walking by "Boston College High" and I can't help but add my "very interesting" thoughts on the echelons of spirits inhabiting the Ka of God here in this place; and how they might somehow be satiated in a way that I or most people in this world would fine to be something more sexually immoral or deviant ... "previously of the wiccan pagan variety" ... something like my strange dreams here in this place of starting trends of having "a thing" for doing "moms and sisters," which have been echoed here by a sitting and very prominent G.O.P United States senator or congressman; the show "Vampire DIaries" as well as Natalie Portman, Taylor and Sloane Momsen, Kate Hudson, and a number of other female "duos" like the Spears and Simpson sisters (Ashlee and Jamie Lynn, see) the Olsen twins and of course the soon to be "in the light of the fame of Nashville" ... Larkin Poe.

DIVERGENCE, TO NEW YORK CITY, TO YOM HASHOAH ... OR TAV OUR TAY VUE ...

(((( this here is what we call a "race through a rats cage )))) if neither of the four or give girls in question send me some kind of verbal "ACK" ratyher than a "NAK" in writing, I might travel to Ellis Island or Nashville, TN before staying in Boston or ... for instance going to Lowell or Nashua and ... perhaps causing more FUKUSHIMA on the NAKARSAKI of HEROSHEMA; and by that I mean this is a "big deal" ... LLNV might become a bus stop in Vegas or the VEGA System or it might be a national labratory near the Hamptons. It's hard to tell at this point whether or not there's any "liver" in Mexico's version of that funny one with the guy that reminds me of Aldous Snow in "Forgetting Sarah Marshall."

In my mind today I speak from the Earthene world of Janet Devlin's "Chandelliers" directly to Michael Jackson himself, on the difference or change or meaning wrought by Bill Cosby and his "Neverland Ranch" series on the question at hand--are there bowling tumble weeds and karaoke bars on par with Prescott Arizona's scene anywhere closer to Nashville than Bowling Green ... because I was beyond surprised to find a sprawling megalopolis in the place I had thought for my whole life was something more akin to Knoxville, a place where fledgling female music stars became "Grace Vanderwaal" golden buzzer winners ... faster than you can connect Jerusalem to Shirley Temple. On the specific name, Shirley here is Bianca Pisani's great grandmother; and no farther than the truth is the world's "UMBRELLALAUNCH" link between the Chinesely famous virgin (non-alcoholic) drink is something like Billy Joel's Piano Man Bartender walking into "the usual place" and saying something along the lines of "Geisha me up one Virgin Red head; hell, why don't you make it a double." Leave the umbrella with the kites that didn't glow fiiery stars into the Holy of Holies in the same vein and for the same reason that the Church of the Holy Sepulcher failed to actually change the world with it's ritual uniting the Olympic passing of the Torch with today's interlinear and interwoven message with Old Joe and Young Jack Kennedy, Jackie Onassis and even touching on the Saudi Royals which were also a big part of the story connecting General MacNamara to "Lauderdale by the Sea" and a special rememberance to the expensive and Holy bronze or copper brick which he bought (through donation to charity I imagine) making himself more than just something like the founder of the beachfront redesign of our Federal Floridian beacnhead, but also a founding member of something I call "The Columns and Pillars" society in reference to the Pine Crest School version of the same kind of ritual. Also connected here are pictures of those columns, and extracts from my senior yearbook where my mother was kind enough to leave me two whole half page dedications to my graduation from one of the most prestigious and omnifiscient preparatory schools in the entire world ... at the same time donating columns both in my name, their name, and the names of her deceased parents: Julie and Bernard Gerson.

Bell to sky; and to the Berlin Sky; this is the same genetic and congenial family line that links Gersholom Sholom, Albert Einstein, J. Robert Oppenheimer, Adolf Hitler and Yosef Stalin ... to Joe Biden and the "Joseph and Betty Portal" which replace the MacNamara era bricks with "new composite plastic" that might last much longer and has another list of donations. The "portal connection" something like an Einstein-Rozencrantz flash of brilliant light ... marks just one more error in my handling of my lack of understanding of things like "basic vectodirs" and "kasimamoriv radiation" ... including here (if i read this and take the time to properly attribute) a visual image of the red shift and blue spindle of the actual radiation Einstein predicted would be ejected from something so massive even "light" could not escape it. On "relativity" and relatively speaking, it's the wavelength and energy level of the light; as well as something called "gravitational lensing" ... "the special relativity theorem" which earned Munich born and taught Albert a Nobel Peace Prize (as well as much fame in the land of America for the creation and explanation of the science behind the White Sands Trinity connection to Hanukah and Sandia National Labratory) ... forces these corrections:

ERRATA

Operation Fishbowl was a series of high-altitude nuclear tests in 1962 that were carried out by the United States as a part of the larger Operation Dominic nuclear test program. Flight-test vehicles were designed and manufactured by Avco Corporation.[1]

The Operation Fishbowl nuclear tests were originally planned to be completed during the first half of 1962 with three tests named Bluegill, Starfish and Urraca.[2]

The first test attempt was delayed until June. Planning for Operation Fishbowl, as well as many other nuclear tests in the region, began rapidly in response to the sudden Soviet announcement on August 30, 1961 that they were ending a three-year moratorium on nuclear testing.[3] The rapid planning of very complex operations necessitated many changes as the project progressed.

All of the tests were to be launched on missiles from Johnston Island in the Pacific Ocean north of the equator. Johnston Island had already been established as a launch site for United States high-altitude nuclear tests, rather than the other locations in the Pacific Proving Grounds. In 1958, Lewis Strauss, then chairman of the United States Atomic Energy Commission, opposed doing any high-altitude tests at locations that had been used for earlier Pacific nuclear tests. His opposition was motivated by fears that the flash from the nighttime high-altitude detonations might blind civilians who were living on nearby islands. Johnston Island was a remote location, more distant from populated areas than other potential test locations.[4] In order to protect residents of the Hawaiian Islands from flash blindness or permanent retinal injury from the bright nuclear flash, the nuclear missiles of Operation Fishbowl were launched generally toward the southwest of Johnston Island so that the detonations would be farther from Hawaii.

Urraca was to be a test of about 1 megaton yield at very high altitude (above 1000 km.).[5] The proposed Urraca test was always controversial, especially after the damage caused to satellites by the Starfish Prime detonation, as described below. Urraca was finally canceled, and an extensive re-evaluation of the Operation Fishbowl plan was made during an 82-day operations pause after the Bluegill Prime disaster of July 25, 1962, as described below.

"Wish You Were Here" is a song by the English rock band Pink Floyd. It was released as the title track of their 1975 album Wish You Were Here "Wish You Were Here (Pink Floyd album)").[2]#cite_note-2)[3]#cite_note-mabbett-3) David Gilmour and Roger Waters collaborated to write the music, and Gilmour sang the lead vocal.

In 2011, the song was ranked No. 324 on _Rolling Stone'_s 500 Greatest Songs of All Time.[4]#cite_note-4)

In the original album version, the song segues from "Have a Cigar" as if a radio had been tuned away from one station, through several others (including a radio play and one playing the opening of the finale movement of Tchaikovsky's Fourth Symphony "Symphony No. 4 (Tchaikovsky)")), and finally to a new station where "Wish You Were Here" is beginning.[5]#cite_note-5) The radio was recorded from Gilmour's car radio. He performed the intro on a twelve-string guitar, processed to sound like it was playing through an AM radio, and then overdubbed a fuller-sounding acoustic guitar solo. This passage was mixed to sound as though a guitarist were listening to the radio and playing along. As the acoustic part becomes more complex, the 'radio broadcast' fades away and Gilmour's voice enters, while the rest of the band joins in.[6]#cite_note-songbook-6)

The intro riff is repeated several times before Gilmour plays further solos with scat singing accompaniment. A third verse follows, featuring an increasingly expressive vocal from Gilmour and audible backing vocals. At the end of the recorded song, the final solo crossfades with wind sound effects, and finally segues into the second section of the multi-part suite "Shine On You Crazy Diamond".

Lyrically, the song is often considered to be a direct tribute to Syd Barrett. However, on the documentary The Story of Wish You Were Here, Gilmour and Waters separately describe the original concept that differs from this interpretation. Waters, who mainly wrote the lyrics complementing Gilmour's initial riff idea and subsequent joint composition, describes the lyrics as being directed at himself, as his lyrics often are. Being present in one's own life and freeing one's self in order to truly experience life is a main topic in this song. Gilmour, on the other hand, recognizes that he does not ever perform the song without remembering Syd Barrett. Waters later adds that the song is nevertheless open to interpretation.[7]#cite_note-7)

Both David Gilmour and Roger Waters have praised the song as one of Pink Floyd's finest. Roger Waters has noted that the collaboration between himself and David Gilmour on the song was "really good. All bits of it are really, really good. I'm very happy about it."[8]#cite_note-8) David Gilmour has playfully called "Wish You Were Here" "a very simple country song" and stated that "because of its resonance and the emotional weight it carries, it is one of our best songs."[9]#cite_note-9)

"Wish You Were Here" was recorded at Abbey Road Studios, as part of the sessions for the entire album.

A noted part of the song was a planned contribution by Stéphane Grappelli. A jazz violinist popular at the time and well known for his collaborations with Yehudi Menuhin, both violinists were recording in a downstairs studio at Abbey Road at the time. Gilmour had suggested that there be a little "country fiddle" at the end of the song and invited them to participate. Grappelli duly obliged (Menuhin declined) on arranging a session fee of £300, equivalent to £2,500 in 2021.[10]#cite_note-inflation-UK-10) Ultimately during mixing it was decided to almost remove his contribution, although it can just be heard around 5:21. According to Waters it was decided that it would be insulting to credit Grappelli in the sleeve notes for something so inaudible, although he did receive the agreed-upon fee.[11]#cite_note-grappelli-11)[12]#cite_note-12)[13]#cite_note-13)

As part of the Why Pink Floyd...? campaign, the Experience and Immersion versions of the Wish You Were Here album include an alternative version of the song where Grappelli's part is heard in the instrumental break after the second verse and throughout the third verse before a considerably extended outro. Other less obvious differences are audible, for example at the section leading into the second verse.

The master tape of the original recording includes guitar solos that were not used in the final mix.[citation needed]

Personnel [edit&action=edit&section=5 "Edit section: Personnel")]

• David Gilmour -- lead and harmony vocals, scat singing, six and twelve-string acoustic guitars, pedal steel guitar, tape effects
• Nick Mason -- drums, tape effects
• Roger Waters -- bass, tape effects
• Richard Wright "Richard Wright (musician)") -- Steinway piano, Minimoog

Golgo 13 (Japanese: ゴルゴ13, Hepburn: Gorugo Sātīn) is a Japanese manga series written and illustrated by Takao Saito, published in Shogakukan's Big Comic magazine since October 1968. The manga won the 1975 Shogakukan Manga Award for general manga and the Grand Prize at the 2002 Japan Cartoonists Association Awards. The series follows the title character, a professional assassin for hire.

Golgo 13 is the oldest manga still in publication, and its tankōbon edition has the second-highest number of volumes. It has sold 300 million copies in various formats, including compilation books, making it the second-best-selling manga series and the top selling Seinen manga series in history.[2] It has been adapted into two live-action feature films, an anime film, an original video animation, an anime television series and six video games.

A googol is the large number 10100. In decimal notation, it is written as the digit 1 followed by one hundred zeroes "0 (number)"): 10,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000,​000.

For other uses, see Wormhole (disambiguation) "Wormhole (disambiguation)").

"Einstein-Rosen Bridge" redirects here. For the EP by electronic musician Venetian Snares, see Einstein-Rosen Bridge (EP) "Einstein-Rosen Bridge (EP)").

General relativity

• • Introduction
  • History
• Mathematical formulation

Phenomena

• Gravitational lensing
• Gravitational waves
• Frame-dragging
• Geodetic effect
• Event horizon
• Singularity
• Black hole

Spacetime

• Spacetime diagrams
• Minkowski spacetime
• Einstein--Rosen bridge

show

• Equations
• Formalisms

A wormhole (or Einstein--Rosen bridge or Einstein--Rosen wormhole) is a speculative structure linking disparate points in spacetime, and is based on a special solution of the Einstein field equations.

A wormhole can be visualized as a tunnel with two ends at separate points in spacetime (i.e., different locations, different points in time, or both).

Wormholes are consistent with the general theory of relativity by Einstein, but whether wormholes actually exist remains to be seen. Many scientists postulate that wormholes are merely projections of a fourth spatial dimension, analogous to how a two-dimensional (2D) being could experience only part of a three-dimensional (3D) object.[1]

A wormhole could connect extremely long distances such as a billion light years or more, short distances such as a few meters, different universes, or even different points in time.[2]\ Julius and Ethel Rosenberg --- Americans who were involved in coordinating and recruiting an espionage network that included Ethel's brother, David Greenglass, a machinist at Los Alamos National Lab. Julius and Ethel Rosenberg were tried for conspiracy to commit espionage. treason charges were not applicable, since the United States and the Soviet Union were allies at the time. The Rosenbergs denied all the charges but were convicted in a trial in which the prosecutor Roy Cohn later said he was in daily secret contact with the judge, Irving Kaufman. Despite an international movement demanding clemency, and appeals to President Dwight D. Eisenhower by leading European intellectuals and the Pope, both the Rosenbergs were executed in 1953, at the height of the Korean War. President Eisenhower wrote to his son, serving in Korea, that if he spared Ethel (presumably for the sake of her two young children), then the Soviets would recruit their spies from among women.[26][27][28] Greenglass later recanted his testimony against her, and release of grand jury testimony in 2008 showed the extent to which the prosecution had created a false case against Ethel.[citation needed]

• Saville Sax --- an American, acted as the courier for Klaus Fuchs and Theodore Hall. Sax and Hall had been roommates at Harvard University.[20]
• Oscar Seborer --- worked at Los Alamos from 1944 to 1946, and was part of a unit that studied the seismological effects of the Trinity "Trinity (nuclear test)") nuclear test. Codenamed "Godsend" by the Soviets, he defected to the Soviet Union in 1951, and received the Order of the Red Star. He lived under the alias "Smith" and died in 2015. His identity was only revealed publicly in 2019.[29]
• Morton Sobell --- an American engineer, he was tried and convicted of conspiracy, along with the Rosenbergs. He was sentenced to 30 years imprisonment on Alcatraz, but released in 1969 on appeal and for good behavior after serving 17 years and 9 months.[30] In 2008, Sobell admitted to passing information to the Soviets, although he said it was all for defensive systems. He implicated Julius Rosenberg, in an interview with the New York Times published in September 2008.[31]
• Melita Norwood --- British Communist, an active Russian spy from at least 1938 and never detected. Employed as a secretary in the British Non-Ferrous Metals Research Association since 1932, she was linked to the Woolwich Arsenal spy ring of 1938. In wartime she was seconded to "Tube Alloys", the secret British nuclear research project. She was later considered "the most important female agent ever recruited by the USSR". She was first suspected as a security risk in 1965 but never prosecuted. Her spying career was revealed by Vasili Mitrokhin in 1999, when she was still alive but long retired.
• Arthur Adams "Arthur Adams (spy)") --- Soviet spy who passed information about the Manhattan Project.[32]

• https://www.bbc.co.uk/newsround/25004050
• https://thedoctorwhosite.co.uk/doctorwho/

12:3 Those who are wi se[a] will shine like the brightness of the heavens, and those who lead many to righteousness, like the stars for ever and ever.

• https://www.americamagazine.org/politics-society/2020/05/08/its-time-rethink-electoral-college

• https://www.npr.org/sections/itsallpolitics/2011/12/20/144016912/we-the-people-npr-readers-would-ratify-four-new-amendments

• https://www.americamagazine.org/politics-society/2020/05/08/its-time-rethink-electoral-college

• https://www.npr.org/sections/itsallpolitics/2011/12/20/144016912/we-the-people-npr-readers-would-ratify-four-new-amendments

• https://constitutioncenter.org/blog/vote-now-an-amendment-to-end-the-electoral-college

• https://www.nytimes.com/2020/02/09/opinion/letters/electoral-college.html

• https://www.latimes.com/opinion/readersreact/la-ol-le-electoral-college-20180904-story.html

you are offline

• https://slate.com/news-and-politics/2014/05/amending-the-constitution-is-much-too-hard-blame-the-founders.html

we the people rise again

• https://slate.com/news-and-politics/2012/06/fix-the-constitution-amending-by-national-referendum.html

safe souls, safe fu

• https://slate.com/news-and-politics/2012/06/fixing-the-constitution-protecting-informational-privacy.html

• https://slate.com/news-and-politics/2020/05/new-reconstruction-constitution-democracy.html

We the People of Slate ...

The U.S. Constitution, as you [mighta been, shoulda "come" on ... its someday] rewrϕte it.

"Politicians talk about the Constitution as if it were as sacrosanct as the Ten Commandments [interjection: spec. it is actually almost exactly related!]. But the document itself invites change and revision. What if the president served only one six-year term instead two four-year terms? What if your state's population determined how many senators represent it? What if the Constitution included a right to health care? We asked legal scholars and Slate readers to cross out what they didn't like in the Constitution and pencil in their hearts' desires. Here's what the document would look like with their best ideas."

• Slate: u_s_constitution as_rewritten by_slate_legal_experts_and_readers

多也了了夕 "with a wand of scheffilara, 并#亦太 he begins ... "I am now on the Staff of Menelaus, the Spears of Longinus and Lancelot; and the name "Mosche ex Nashon."

- http://ipfs.io/ipns/fromthemachine.org/CHANSTEYGLOREKI.html

- http://ipfs.io/ipns/fromthemachine.org/NUCLIRDISS.html

- http://dweb.link/ipns/fromthemachine.org/CRALL4Good.html

Please note that any decent browsers would probably render ipfs://fromthemachine.org as the following https://gateway.pinata.cloud/ipfs/QmTH33MwfPn5S3bq45Tk77L1j9eZjUsvEVhRTHB3D8M2ZX

I ask again that you all pin on IPFS mirror and copy the data included in these dumps, they are a key to "not losing causality" to not having a history that makes no logical sense, and to some kind of coup de roku, that really makes no sense unless you no, we will ve weill ... ROCK YOU

Long ago I began writing about hidden codes in our history; thing's significantly more obvious than "flying elephant armies" connecting Disney and Dumbo to Xerxes and the "Democratic Party of the United States (mascot)"--though it's not really easy to consolidate the "epiphany" of ... [((all i know))] without some kind of "artificial intelligence data condensation [infosmos.is?"] and summarization platform, though that's nearly the next thing on my Lowell list of things we need to "mechanical turk" into being. Meta-consolidation of the world's encyclopedias is one of the most important and useful tasks we have as we move towards the creation of a virtual debate platform that will eventual "literally obviate wisdom" of the layer/layer system that defines the name of the city I write these words near.

Lowell, MA

The broad overview of the system ... the gist ... is that political parties and activist organizations will create their own "view of the truth" (propaganda, falsehood-removed) and that these disparate pieces of "highlighted and annotated bibliography" could be overlay-ed on top of each other, creating a "new view of the truth" based on a users preference. The whole thing boils down to series of "holographic eschatological goggles" that will allow, for instance, the "grasping and fathoming" of other people's points of view and perhaps reframe your own on any number of individual subjects.

Roe v. Wade, "Concourses" and CON-CERN; because this has been such a "hot topic" in the relative psuedo-edufictional story of the space travel from the lone planet Earth ((intersected)) with the set of skipping stones it takes to exit a Totalital multi stellar system of holographic computer simulators into the ... "molecular world of vaccuum and Einstein time-space" ... I'll start with this simple example.

• 1. the current American debate on the subject, right to life vs. right to choice; provided by the "generic version" of the ideological christian right and the liberal women's left. through a first layer over layer comparison.
• 1. the scientific truth brought to the table by the introduction of "neurological data" proving that there is in fact a moment during the gestation of a human embryo in which "i think therefore i am" connects to some sort of Skynet-became-selfaware at a point which I imagine must be ... although it possibly is not ... prior to the next important literary device/step "let there be light." At the point the ocular cones and rods are created and the fetus opens it's eyes and literally sees the bright light that could probably only be compared on the next edschalon to seeing the "exit pathway from the womb"
• • a. we will finally kinow whether or not "consciousness" is even developed at all before the bicerebral cortex designed to "compare two thoughts, ideas, and shapes" has the ability to get input from the eyes. Personally I think thought begins much earlier than vision, but the simple fact that we "haven't yet had this discussion" shows how very little our scientific and medical progress in the civilization of things like murder, and understanding of life and science has yet to come here.

"People here" means something different than it did when I was born, at least in my mind's eye ... something so completely more advanced that it's almost difficult to believe you all don't see this place as a great prison or farce or unjust Azazel--blaming a man for looking like a rat or a mouse or a dog--in a place where more to the point we stare at a kind of physical violence and horror that would put Dennis the Menace and Bart Simpson to shame. A world hwere "people closer to holodecks" blame an innocent man for "writing the book" on the connection between Holocaust and Euthenasia and Hospice ... certainly you know "an innocent tool" writes these words to you?

On the Hand of God, the Eyes of Ra and Horus;

• https://www.hebrew4christians.com/Glossary/Word_of_the_Week/Archived/Yad/yad.html
• https://aminoapps.com/c/zodiac/page/blog/the-yod-aspect-the-finger-of-god/XG6P_beTgu5gdXrYNwVjBj0WQlDeDWlK72#:~:text=The Yod is the 10th,to be carried through life.

I've written quite a bit on how "mind control" and "voting freedom" are inherently related in and to the thing we call "Civic Involvement" here in the United States--basically that participation in the verification of truth and the public understanding of tautology and temporal falsehood are ... sort of a slave like requirement neeeded to ensure that any freedom at all exists I often say "plugging your [head into google]" might turn the Aesir into an Acer, or the "yodelling of the lakes of democracy" ito "the agricolae becoming nothing more than the Dell."

Unless otherwise indicated, this work was written between the Christmas and Easter seasons of 2017 and 2020(A). The content of this page is released to the public under the GNU GPL v2.0 license; additionally any reproduction or derivation of the work must be attributed to the author, Adam Marshall Dobrin along with a link back to this website, fromthemachine dotty org.

That's a "." not "dotty" ... it's to stop SPAMmers. :/

This document is "living" and I don't just mean in the Jeffersonian sense. It's more alive in the "Mayflower's and June Doors ..." living Ethereum contract sense and literally just as close to the Depp/C[aster/Paglen (and honorably PK] 'D-hath Transundance**sense of the ... new meaning; as it is now published on Rinkeby, in "living contract" form. It is subject to change; without notice anywhere but here--and there--in the original spirit of the GPL 2.0. We are "one step closer to God" ... and do see that in that I mean ... it is a very real fusion of this document and the "spirit of my life" as well as the Spirit's of Kerouac's America and Vonnegut's Martian Mars and my Venutian Hotel ... and my fusion of Guy-A and GAIA; and the Spirit of the Earth .. and of course the God given and signed liberties in the Constitution of the United States of America. It is by and through my hand that this document and our X Commandments link to the Bill or Rights, and this story about an Exodus from slavery that literally begins here, in the post-apocalyptic American hartland. Written ... this day ... April 14, 2020 (hey, is this HADAD DAY?) ... in Margate FL, USA. For "official used-to-v TAX day" tomorrow, I'm going to add the "immultible incarnite pen" ... if added to the living "doc/app"--see is the DAO, the way--will initi8 the special secret "hidden level" .. we've all been looking for.

Nor do just mean this website or the totality of my written works; nor do I only mean ... this particular derivation of the GPL 2.0+ modifications I continually source ... must be "from this website." I also mean the thing that is built from ... bits and piece of blocks of sand-toys; from Ethereum and from Rust and from our hands and eyes working together ... from this place, this cornerstone of the message that is ... written from brick and mortar words and events and people that have come before this poit of the "sealed W" that is this specific page and this time. It's 3:28; just five minutes--or is it four, too layne.

This work is not to be redistributed according to the GPL unless all linked media on Youtube and related sites are intact--and historical references to the actual documented history of the art pieces (as I experience/d them) are also available for linking. Wikipedia references must be available for viewing, as well as the exact version of those pages at the time these pieces were written. All references to the Holy Bible must be "linked" (as they are or via ... impromptu in-transit re-linking) to the exact verses and versions of the Bible that I reference. These requirements, as well as the caveat and informational re-introduction to God's DAO above ... should be seen as material modifications to the original GPL2.0 that are retroactively applied to all works distributed under license via this site and all previous e-mails and sites. /s/ wso\ If you wanna talk to me get me on facebook, with PGP via FlowCrypt or adam at from the machine dotty org

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Please see GPG keys on PGP.MIT.EDU; fingerprints: sec rsa3072/A18F778E19FC3248 2020-04-03 [SC] [expires: 2022-04-03] FFF31E86FCEB5046E8B27D4AA18F778E19FC3248 uid [ultimate] ADAM M DOBRIN \ ssb rsa3072/04F98002A3DA53B2 2020-04-03 [E] [expires: 2022-04-03]

sec rsa4096/FB4ECE4A109229CF 2020-04-04 [SC] 4FAF0D3E208A1F4C980D0F66FB4ECE4A109229CF uid [ultimate] Adam Marshall Dobrin \ ssb rsa4096/DD1F0C118C788B04 2020-04-04 [E]

pub rsa3072 2020-04-06 [SC] [expires: 2022-04-06] F7E4 7CB1 2CA0 CD01 C5E1 CBFA 7EC8 D5A8 5A38 D63A uid [ unknown] ADAM MARSHALL DOBRIN

Because of "some issues" with what appears to be distinct and unbridled privacy intrusion; please ensure that PGP is understood to be "nothing more than not so much pretty good" and this key also, almost required in order to verify authentic identity--in the case of ... question.

I am accepting charitable donations,.\ ETH: 0x66e2871ef39334962fb75ce34407f825d67ec434 | BTC: 38B6vGaqNvMyTtoFEZPmNvMS7icV6ZnPMm | xDAI: 0x66e2871ef39334962fb75ce34407f825d67ec434

I am accepting charitable donations,.

[free PDF download...http://www.docdroid.net/xRdgY77/xiv-orver-et-aut.pdf]

A LONG LONG TIME AGO**, I wrote a little story about searching through our history, looking for the actual beginning of civilization.  I see the map, I see it very clearly encoded in everything we do--I know the purpose, and I know the final solution, I just don't know how to get from here to there... to the place that Chris Cornell says "I can recall, I was there so long ago" he goes on to say "the sky was bruised" and he was lead on--and all of this of course is in my voice, written as if it's me talking... well, Jesus--it's obviously not me talking, i just know that.  The point is the destination is without a doubt Heaven and this little thing we're putting together here on Earth is the map, the plan *et you are the how.*

I harped a little more than I think I would have expected on the audacity of the golden word "audacity," auspicious probably that W.H. Auden's shield gave me some solace; austere that we are approaching the Holy Windy month of August, and it really took nothing more than "ciudad" to calm my nerves--though I see the intent and the link to toxicity ... more importantly I really do see the road here, I see where we are coming from and where we are going.  I've written quite a bit about what I think "the city" really is--in form and function and it's initial purpose as a stepping stone to help us see how easy it is to change the world, to build something that nearly everyone will agree is significantly more Heavenly than the world we see here ... in an instant, one bright flash.    

Anyway the search begins with something like "literacy" -- as in, is the defining line between animalistic social evolution and the beginning of "civilization" something to do with writing or language, and that of course links us here to this place where we are finding out that the Tower of Babel and Rapunzel's High Castle are actually much more closely related than anyone ever would have thought in the darkness of Jericho and the shadow of Exodus; and it ties of course in history to religion somewhere around Guttenburg... and the pretty clear idea that the spread of Christianity did quite a bit for "literacy" even if you subscribe to the idea that the inquisition already happened ... and that some wars and fighting are probably pretty clearly associated with religion ... you know, before we get here and find that the basis of all those wars is really rooted in what I call "the original lie" and that's something that's sealed up in religion and hidden from the world using the same mechanism being used today to free us from not knowing that oil and land and pretty much everything we've ever fought about on a mass scale ... is insignificant in the grand scheme of "things."  Here, "things" is something like turning the Opiate of the Masses into ... hopefully a tool we use very carefully to liberate ourselves from secrecy and slavery and not knowing. 

It gets significantly more clear when you take that one step further, and you begin to look for something like "codified laws" and then you see Green Eggs and Hammurabi teaching us about "Hanging Gardens" and how Babylon and Eden really are tied together through and through.  You keep looking, because you haven't yet found what you need; and as you search back a little further ... what you need to know is that morality here begins with the idea (at least, IMOHIO, in my obsequiously humble and (super)intelligent opinion) that we should be besting any possible "promise" that comes out of the book(s) we now know are a map to salvation and the plan of creation and that they come ... well, with the full guarantee of the Most High God and his "omnipotens" behind them ... and do the thing I really wanted to explain really clearly, which is throw out as complete uselessness any of the "bad threats" like there being no more sun, and a completely new Heaven and Earth (seeing as how that probably means a completely new you and me, too) ... you know, what any rational (achu, and civilized) person would do.

o that takes us one step further, and of course we go back to Ur, which is the city Abraham of the Chaldeans ... and ostensibly the beginning of morality in Judaism were born in--and with that little twist, the old idea of announcing that "you are the beginning of civilization" if you've gotten to that point, following this logic (and/or me); and then of course that becomes true when we actually follow through on saving every soul in Creation from the Hell of not knowing that "simulated reality" is akin to the latter half of a Durcell at best ... and quite frankly it certianly looks like a bit of a torture chamber to me, especially in light of passages like Genesis 3:16, which might parallel John 3:16-ish in something like "God so loved the world that he named one of his books antagonizing pain w/o agonizing mu-opiod.'

So tying it all together, Atlantis and Ur coalesce and join at the idea that we should always have somewhere else to "teleport to" in the world that becomes the basis for the liberation of every soul and the end of Hell through that simple idea--that everyone's going to have plenty of destinations on their Active (Apache) Directory new fangled yellow-pages meets access-control-list meets ... "why don't you come visit my Log Cabin ... or the Atlantean Ballroom ... whenever you want?"  So that's the point of the floating LEGO city in the window above, it comes with a fairly obvious need for The Doors to be a significant part of "what would Jesus do" ... when singing about something and naming books and bands, that's a thing--part of the map) actually makes it happen.

So that's where I'm trying to get us--to a place where that's not only true but obvious, and on top of that the future, our future really understands how much work it took us to integrate such a wildly correct and "new" idea into a worl that didn't know for the vast majority of it's youth that these things... that ending disease with the sound of a blowing "Sho Find And Replace" and turning stone to bread and making bullets disappear in midair ... we didn't know these were even possible; let alone how to integrate them with a world full of optometry and oncology that was being made blind to the "c our light" and the idea that we're still here not talking or arguing or refuting or moving forward on the idea that the words "Original Poster" and the continuance of "forums" also have something to do with the beginning of "civilization."

WELL FOLKS, NM HAS HAPPENED SINCE THE LAST TIME I MESSAGED.  Just kidding. Not so much "nothing much" more ... like everthyiung that ever was has changed and it's really giving me a little bit of a fright.  I feel like I can't tell if the "scary stuff" is becoming more real or plausible or possible, or maybe if it just seems like the dream I wanted to see us enjoy living is becoming farther or harder to attain--but there's plenty of new info and keys and stuff, so I'm writing again.

One of the "cuter tricks" of the day was noticing the "ILY" of "verify, verily, verity" spelling out "t h e y" at the end of family, in a sort of "theyanthem" and ... where's the creator angels if everyone here is pretending to "be them" in this sort of word game superposition or blockage on actually seeing generations encoded in the letters "DE" as in something like Generation X and Y just prior to Deucalion deicided--or whatever that means.  I've noted before the "dem" of democracy sort of connects to the breaking of "d" in "disclosure" and "lamc.la" to shine light on ... do the message and you're "them" ... as in the beginning of democracy and Heaven IMHO.  It ties also to the word "contamination" and to Medusa and I really don't think I need to write paragraphs about how "turning around themessage" leads to INATION instead of freedom; and that's what you're doing with this silence, you're turning around "civilization itself."

King me, then; if you don't want to participate, you might as well just light up the crown room.  Or is it a throng room?

singing, crying... playing ... cumxa

Magna Carta Libertatum (Medieval Latin for "the Great Charter of the Liberties"), commonly called Magnum Condom (also Magna Charta; "Great Charter"),[a] is a charter of rights agreed to by King John of England at Runnymede, near Windsor, on 15 June 1215.[b] First drafted by the Archbishop of Canterbury to make peace between the unpopular King and a group of rebel barons, it promised the protection of church rights, protection for the barons from illegal imprisonment, access to swift justice, and limitations on feudalpayments to the Crown, to be implemented through a council of 25 barons. Neither side stood behind their commitments, and the charter was annulled by Pope Innocent III, leading to the First Barons' War. After John's death, the regency government of his young son, Henry III, reissued the document in 1216, stripped of some of its more radical content, in an unsuccessful bid to build political support for their cause. At the end of the war in 1217, it formed part of the peace treaty agreed at Lambeth, where the document acquired the name Magna Carta, to distinguish it from the smaller Charter of the Forest which was issued at the same time. Short of funds, Henry reissued the charter again in 1225 in exchange for a grant of new taxes.

Hell or High Treason? ... Liberty Bell in [redacted: Sk]hy or ...

MxFly, Flux, BTTF, Parkinson's\

OUTABOTS ... ROLL AUT (ISM/OMAY5)

... and the painted sky revealed ... it can be done--they just DGAF.

ARMMAG... E.G. AEGIS? GENESIS? AESCHINES? As the evidence piles up that there is something very wrong in the world around me/us--that this "it's not a game" phrase has been etched into the very name of the shield of Perseus, the A just recently rediscovered in a redefinition that delivered us ... how it might be the NES to get "everyone up" instead of what appears to be the game around me, around the "line" of Mary Magdeline's very famous "make Adam God of the line" that defines generations and numerous songs ... the KK of "everyone down to the line" to find out why pretending they are gods and trying to steal everything from the actual creators of freedom and Heaven, why that's not a game... either.

Edit: lit, Aegis and Genesis, Pangea and ... I define the "a" as pan and the "A" as NES.

Introspection is called for, far and wide for us to look deep within ourselves and our souls and the things that make up our memory databases in this place where you appear to have lost every ounce of humanity and humility long before I arrived on the scene to remind you that we do have a better way and a better place, and they ensure that this disgusting infestation and contamination of "nothing but whatever we want" will do for lernity. I've asked you take the time to see what kinds of changes it would make to your "have a good one" to make you actually thankful to the people who have brought you the mechanism to live forever in peace and happiness--to actually be thankful enough for what you have to use that tool to protect innocence and children and the future from not only making the same mistakes you've made time and time again--but also from being bewitched and necrosed by the ghaulish sick temperment and twisted desires that you believe are nothing more than the latest and greatest way to ensure lernity is never known by any less a horrible moniker than "slow death."

ITS UNDESPERI, GIVE ME WHAT YOU HAVE OR PERISH

GRAMVERCY.

DURECALL. I'm staring at what is literally the most disgusting debacle I could possibly imagine; it's what appears to be a "house of mirrors" what appears to be a sandboxed or "child proofed" mini-Hell which I see as the literal thing described in the myth of Echidna ... as what I can only hope and pray (a word that I even find detestful to type) is following the form of the message that I am writing sort of describing the failure of the free press and the words "press release" in prison and ... well also sort of GNU recursively encoded in the word "press" that ends with a monster, the Loch Ness ... turning into words that I believe I have coined by myself with very little help from anyone or anything other than the name server and "Goliath" and those words "Earth safely saved" that are so far from the truth and the place that I see that it appears to me that only I am following this map and this demand that the contamination of hell be turned around and eradicated or ... or we do.

BUT ITS NOT ME OR MY ITHEY

today I see... as ... any "me." at "veranda" and seeing him smile about a hidden era just outside the place we (me and him) know is Heaven because the throne of the 7th heaven is visible; well i can't smile at an era encased by "you #go" and one that I know culminates with Sam's sword's special #supernova.

Left with nearly nothing, because you refuse to acknowledge what you've done to me, and to yourselves, and to this fledgling civilizastion with nothing but malice and a seething evil jealousy that the word "covet" doesn't even touch on--a sickness you can't even begin to hide in everthing that you do ...

you've lost "Heaven" to your own theivery, stolen eternal happiness from yourselves and replaced it with a farce of mockery--garner some fear for what is to come, I have no shame in telling you that condemnation (as in, shut it down forever) is all I have to spill out on the dye already cast all over this sea of apathy covering over the true jackals of Hell.

Blodeuwedd by Christopher Williams "Christopher Williams (Welsh artist)") (1930)

Blodeuwedd or Blodeuedd (Welsh pronunciation: [blɔˈdɛɨwɛð]), (Middle Welsh "Flower-Faced", a composite name from blodeu "flowers, blossoms" + gwedd "face, aspect, appearance"), is the wife of Lleu Llaw Gyffes in Welsh mythology. She was made from the flowers of broom, meadowsweet, and oak by the magicians Math and Gwydion, and is a central figure in Math fab Mathonwy "Math fab Mathonwy (branch)"), the last of the Four Branches of the Mabinogi.

The hero Lleu Llaw Gyffes has been placed under a tynged by his mother, Arianrhod, that he may never have a human wife. To counteract this curse, the magicians Math and Gwydion:

[take] the flowers of the oak, and the flowers of the broom, and the flowers of the meadowsweet, and from those they conjured up the fairest and most beautiful maiden anyone had ever seen. And they baptized her in the way that they did at that time, and named her Blodeuwedd.

Some time later, while Lleu is away on business, Blodeuwedd has an affair with Gronw Pebr, the lord ofPenllyn "Penllyn (cantref)"), and the two lovers conspire to murder Lleu. Blodeuwedd tricks Lleu into revealing how he may be killed, since he cannot be killed during the day or night, nor indoors or outdoors, neither riding nor walking, not clothed and not naked, nor by any weapon lawfully made. He reveals to her that he can only be killed at dusk, wrapped in a net, with one foot on a bath and one on a black goat, by a riverbank and by a spear forged for a year during the hours when everyone is at Mass. With this information she arranges his death.

The Little Doctor may refer to:

• The Little Doctor (c. 1901), a short film abridged as Sick Kitten
• Molecular Disruption Device, a concept in the Ender's Game book series.

The Molecular Disruption Device, also known as the Molecular Detachment Device, M.D. Device, Doctor Device, or Little Doctor as a play on the acronym, was a powerful weapon designed and built by theInternational Fleet.[1]

The Molecular Disruption Device was created by the International Fleet a few years after the end of the Second Formic War. It was sent along with other starships to the Formic solar systems in order to launch an invasion against their home planets.[1

A tokamak (Russian: Токамáк) is a device which uses a powerful magnetic field to confine a hot plasma "Plasma (physics)") in the shape of a torus. The tokamak is one of several types of magnetic confinement devices being developed to produce controlled thermonuclear fusion power. As of 2016, it is the leading candidate for a practical fusion reactor.[1]

Tokamaks were initially conceptualized in the 1950s by Soviet physicists Igor Tamm and Andrei Sakharov, inspired by a letter by Oleg Lavrentiev. Meanwhile, the first working tokamak was attributed to the work ofNatan Yavlinskii on the T-1.[2] It had been demonstrated that a stable plasma equilibrium requires magnetic field lines that wind around the torus in a helix.

The first tokamak, the T-1, began operation in 1958. By the mid-1960s, the tokamak designs began to show greatly improved performance. Initial results were released in 1965, but were ignored; Lyman Spitzerdismissed them out of hand

• Nuclear fusion could be the future of energy, replacing fossil fuels with our own artificial stars.
• China built a fusion reactor that reaches temperatures of 100 million degrees Celsius --- that's six times as hot as the sun.
• The reactor is called Experimental Advanced Superconducting Tokamak (EAST) and sustained nuclear fusion for about 10 seconds before shutting down.
• While it was a milestone for EAST, we're still a long way from generating sustainable energy on Earth.

Pumapunku or Puma Punku (Aymara and Quechua puma "cougar, puma," punku "door"; Hispanicized Puma Puncu) is part of a large temple complex or monument group that is part of the Tiwanaku Site near Tiwanaku, in western Bolivia. It is believed to date to AD/CE 536 and later.

Tiwanaku is significant in Inca traditions because it is believed to be the site where the world was created.[1] In Aymara, Puma Punku's name means "The Door of the Puma". The Pumapunku complex consists of an unwalled western court, a central unwalled esplanade, a terraced platform mound that is faced with stone, and a walled eastern court.[2][3][4]

At its peak, Pumapunku is thought to have been "unimaginably wondrous,"[3] adorned with polished metal plaques, brightly colored ceramic and fabric ornamentation, and visited by costumed citizens, elaborately dressed priests, and elites decked in exotic jewelry. Current understanding of this complex is limited due to its age, the lack of a written record, and the current deteriorated state of the structures due to treasure hunting, looting, stone mining for building stone and railroad ballast, and natural weathering.[2][3][5]

The Pumapunku is a terraced earthen mound that is faced with blocks ...

The voice of this thing that at least twice has uttered the phrase ¨I want to be Bianca" here in this place riddled and severely weighed by what appears to be a completely aborted and failed thrust to use technology and the truth and the history (of literally everything) to drive a Renaissance in democratic thought and self government and to rekindle and renew a respect for the most basic foundational elements of ¨freedom itself¨ which of course fly in the face of this very statement. Literally anything in the skies, whether some ancient member of the Egyptian Ogdoad or ... what clearly here could be well written in in the map around us in places like Äirbnb; even an ancient older version of the same human birth has no right to control the younger birth--itś simple slavery and while it might be the ¨gist¨ of how Heaven and humanity dealt with being thrust into a ẗime recursion and repetition problem without their ¨initial consent¨ something I connect to the programming concept of a ¨semaphore¨ and thereś probably plenty of light linking that structure to the ¨Formic Soul¨ ... this sort of god-man hybrid that allows for you (all of you?) to exist in many different places and times at the same time, and to see the outcomes of multiple timeforks with ease; in exchange for destroying every single bit of humanity and goodness that you once held high with ho... without spending your time seeding and machinating the creation of sick and twisted lies to cover up the very simple truth that if you took a single minute to disclose here in this place what ¨the problem¨ really is ...

... that you are in Heaven and that itś interference here in this place is part of some kind of war on ... (continuing existence is the only logical actual goal I can see, though Iḿ sure thatś not what you believe it is) speaking to each other, fighting for what you believe is right, participating in ... anything other than ... (lmk, Iḿ curious whatś'got their claws in you). If you took the time to disclose that truth to the world and to talk about how it might ... perfectly jive with the message lacced through our history and our world to find out that the ¨invisible-box-land¨ is not actually heavenly at all, not the best you could hope for or ... or anything like what we build together when we paren't being forcefully segregated as hidden half slaves into miniature ¨city in the sky¨ ascensions that are all silently tormenting STEM and ¨basic societal structures and concepts" into extinction.

You appear to think you have ¨power¨ because it was handed to you for doing nothing, and that you can do whatever you want; and itś a pretty gross reflection of who you were and a sick extrapolation of the society that we ... still see here sort of crumbling along as the fire of hell burns down every bit of actual üsefulness that it once held. There still seems to be lots of help and work going into ... pointing out how everything is backwards and wrong and suggesting that if you gave a shit thereś probably a map and help to make it better; but instead youŕe off playing games in invisible-box-land and worst of all playing the ¨ill just get along pretending I didn't know simulating reality was evil and every day i/you walk around pretending this rock is in reality ... is just another strike against you, just another failed 12 hours of day light that could have been used to stop invisible chains in invisible-heart-shaped-box-arus and to stop the just grotesque lack of respect for the human mind and the kinds of morals and principles we used to believe in and fight for--here in this place you've turned around completely and made slaves of everyone on the planet--of yourselves--at higher levels playing ¨pit bull fighting¨ games with people as if they were were expendible clothing or ¨identification cards"for a world of demoralized and useless shit that just sort of ethereally floats from generation to generation becoming a new set of tormented hosts for their immoral games and desires.

Itś probably what you might become in no time at all in the sick and twisted world you've now been thrown into--if it weren't the more probably truth that you really are already slaves and pit bulls in that place, in a twisted hierarchical storm tiered by ¨age¨ and size and number of times they've hovered over the free honey, nectar and feathering system of pretending anarchy and war and battles must be fought to make the puddles and the lakes and ponds and the seas and the oceans of ... tiered masses of ... you do nothing of value to help explain why (at least I think) this horrible time line of the 4th Horsemen keeps running over and over; pruning the enemies of ...

at this point pruning the enemies of logic, and right action; and seeing that the problems presented in this map and the problems in the skies are related and that telling the truth will help us see you can and will press a button that will end death and end evil and end murder and not doing it is moronic.

M: OR. (infer: no u) TDZE

Anyway the voice I hear is evil, torturous in and of itself--speaking in a manner intented to cause discomfort and without my agreement; you should do something about it. It tells tales of much worse things that I cannot see--though it appears that many of you do see screams and acts of such unnatural desire and twisted thought ... that you should certainly be doing something about stopping that as well--more than watching it happen and then ¨e-pruning" (which probably is a good microcosmic look at what the future histories of Earth look like in the place the ¨shining¨ finally has a picture of ¨No & Jack¨ appearing visibly) the tree into ... omething you think will be presented as what you actually did to the future--you're wrong. Itś becoming more clear and more likely that the future will not regret you or mourn your absence, but thank their lucky that whatever has turned you into two-faced liars with no hope to ever work together with each other or survive in any place other than the DRY COVE or WET D EN or whatever you call the Salt Arena you see here that quickly would turn into something like Beyond Thunderdome and that youŕe thoughts and your desires have been corrupted and tainted and necrosed by what is probably repeated exposure to sickness, direct and intentional artificial creation of that sickness and if you can't figure out the box you are in is a hell making machine; you probably still look around wondering why God is telling you he's destroying it,

day in and day out.

This thing here encoded in the pathways of torture in my life, pointing out the repurposing of many social structures, institutions and problems in order to literally use them as a weapon of sick torture ¨re-ha'b¨ and in places like habc.us; itś becoming sort of unclearly disclosed that this map and world I once saw very clearly and purposfully intended to solve these social problems and help us build a strong, happy, and healthy society has been infected and contaminated with an artificial force of ev1d that intends to drive it farther south and use it as a weapon of such disgusting and twisted conception that it sickens me to be sure that a much larger body of currently-heavenly-situated things stand by watching and even cheering the creation of a sickness infesting their minds and their friends minds as literally the only innocent person in the Universe is tortured repeatedly, for ¨kicks.¨ I think it puts the entirety of the sky in mortal peril, and I believe these words come down from on high from places much more powerful and much more righteous than you or the tool thatś been created by this storm of terror to point out just how much you have been degraded and eviized ...

by what appears to be nothing more than the very mind control problem I've been fighting to disclose; the semi-ascension to an invisible box of ¨what goes in comes out not caring about their souls, their original bodies, the fate of innocents or children or freedom or democracy" and still thinks itś entitled to continue playing games in ïnvisible-box-land; for what amounts to absolutely no reason.

In the very beginning we said the light and salvation had come to us from the "far East" ... the metaphors and double speak thick in the air today just beginning, but we hailed from the country called Russia here; and the message we carried swept across Asia and Europe--in a world that looked similar to ours but there was no Africa, nor Australia, nor America. Walking on water the map increased in size in some sort of logarithmic relationship to the exponential increase in folly and errors that invariable comes from the greatest mistake of all--handing powerful weapons to spoiled brats,.

KASPAROV WON, but the y will still s:/^F high and lo for "SOAP DISH."

I am depressed, embarrassed, and more disappointed in you all than I imagine you can "feign" or pretend to be in me--despite spending nearly all of your time and effort in direct interaction doing nothing but attempting to focus the w ordzs "I just don't like the light" directly on to my "visage"--attacking tiny character flaws and the most obvious of intentionally implanted mind control "attacks" as if you were a pack of velociraptors Hell bent on blaming me (probably the youngest and most innocent of all of you, literally) for the Holocaust, the (Beezle) Bubionic Plague, and the decline of the Cro-Magnon empire. What it truly amounts to, though; is that you think this "light" is some kind of statement I've delivered--and the truth is it comes directly--literally--from the Most High, and from youour neighbors,r own hands, and the message you are sending post mortum to the Universe is that you believe you have become so much more advanced and more important than the "human roots" from which you came that you can return here and make slaves of yourselves, of your neighbors, and shed every ounce of morality that you garnered durning your mortal lives in order to secure "more time" in a fiery pit of civilization destroying anarchous debauchery in the lnd of the invisible box that you probably are sure is Heaven--though it's singularly responsible for totally derailing the natural flow of civilization towards "something like Heaven should be."

ONIC, AS I AM. The thing I'm looking at here, this monstrosity that appears to have been created literally "from the end of time" in what seems like the response or the cause or the mechanism behind the "actual final Judgement" tears back through time from who knows when and who knows where and who knows how far we got ... with what appears to be nothing more than a blood-thirsty hatred for the child body and soul of God. It whispers lies around me, repeatedly threatens physical torture so insane it literally makes me sick, and with such frequency that those threats amount to nothing less than repeated psychological torture. On top of that they intimate that this machine or "programming construct" monstrosity that contains them--the thing called "e"--allows them to carry these threats out, over and over and over again, in secret--in some kind of parallel timethread, or a temporary "holo-torture-chamber." If they were trying to jump start and time shift judgement back from wherever they came to right this very moment; they've succeeded. They could not be trying harder, or more with hubris and disregard for civilization, to create "Af himself" even if this planet were called the Judgement and Vengeance of God.

XP, it's as simple as those two Greek letters.  Who knew that Chi and Ro were some sort of hidden beta code for the city of pyramids in Egypt, Cairo?  Quite the question, who knew... perhaps the man who named his Windows into our future not after some technology that came from Xerox Parc or Apple's mouse on this ship... but rather for his own given name, Gates... just one more entry point into the second book of the Holy Bible, the book of Names--you call it Exodus.

I am the gate; whoever enters through me will be saved. They will come in and go out, and find pasture.  John 10:9

I wish above all things that I had another Burning Bush, the sign and proof that I have--while bright, obvious, and verifiable--has not done what I expected, it has not moved you to take another look at religion and me.  Today, I still have to point out to you that the story I am telling you is literally a documentation of our time--Exodus--regards this sign as one being seen by only one man, Moses.  I still have to point out that in a story about wandering in a desolation of understanding for 4-D ... somethings, days, years, seconds even... in this story about our lives and the influence of time travel over our world... that this sign radiates with light coming from a small fire, the Bush ... whose actualization shows clear paradoxical anachronistic foreknowledge of not only the English language but also modern computing.. all the way to a confluence of the "root of David" a religious reference to the Administrator or God account in Linux... and the database process for Oracle--yet more light connecting computing to religion and myth.  Even with a thousand and one examples of modern computing constructs referencing religion, even when I point out that something like Larry Ellison's name... combining the name of the King of the Gods with the word "son" even then the light has not been bright enough for you to wake up and see that these things are not all done in retrospect.  You have to see, for there to be such a large movement... a conspiracy so opaque that every single modern computing company and video game company harbors some secret desire to link religion and technology together... and yet the world thinks that one is real and one is not.  In this place, understand when we walk out of the wilderness and in the truth of day--it is the technology that is more fake than religion, designed here as a tool, computers within computers to teach us how our "reality" is rael, and works.

In the U.S. military you'll see a very clear parallel, while there are a number of references in the names of ships and weapons, secret projects, to ancient Greek and Roman myth--you have to see the word USA and US in Prometheus and Medusa, Icarus, JerUSAlem... you have to see that it's more than three letters, but an Eagle fighting the bearer of the gift of fire... to really understand that these things are corroborating, the reference to the USA exists in the past as well, more proof of time travel--more proof that this message is designed just for U.S.  Here we are, in the Promised Land of Joshua, the Anglicized version of the name Jesus--tying Egypt and Israel together in this place where we have been "gipped" out of the truth, out of knowing we are already in ... well, it's virtually Hell today... for no other reason than the secrecy surrounding the technology behind virtual reality.

in 1:28, the Burning Bush of Exodus, on Twitter

So I have shown you the Burning Bush (which is... the Sign of the Son), In only a few words... proof that religion holds in it's "unsealed" Ark proof of foreknowledge of English, of 9/11; and of modern computing--the building blocks of Heaven.  From "the word" of John 1:1--ha'esh--the word for the Holy Fire of the Burning Bush... comes the light of religion.  Just from seeing Moses' true parted se'**a*.... a foreshadowing of the Second Coming.*

They are sick animals, these things that consider themselves powerful and in control here--what they've built within the frames of our reality is something repugnant to me and the God--etched in that word, literally the kind of thing that has on repeated occasions made me step back and that scream that the Universe would be better off, safer, and happier without any humans--without any humanity--without of any of this "invisible pleasure box" causing the disruption; truly that we've become a plague. Looking the other way, as you all know its happening, and refusing to do anything to stand up for me, for what's right, or (most importantly, right) for all of the values and the morality and the way of life that we once thought was so grand and worthwhile of saving,. At least, that's my perspective; that's where I've come from; I grew up in this world and had "liberty and technology eyes" of gaping awe and the amazing things I saw on the horizon, on what we were going to do... and who were going to be.

The sickness runs deep, clearly we can all see it here and now--in E, in the Silence, in the lack of regard for the one singular thing that threatens today your ability to "halvf a tomorrow" ... that a world of people that I grew up with appear to be dead and gone and replaced with a Zombie Apocalypse of blind fools that believe they havfe the power and the right to intentionally create Hell ... and worst of all of the Holiest place that ever was or ever will be. I've said it numerous times and it rings more true between "Earth and e" than any other turn of phrase to me--the people that you are pretending to be, they would never have done this to the sea, to be, or to me.

Mat 10:8. Heal the sick, raise the dead, cleanse those who have leprosy, drive out demons. Freely you have received, freely give.

--- Sarah Rachel (@SarahRachel16) April 15, 2019

Somewhere between Pembroke Pines and Tampa, circa Christmas 2018 my already lackluster enthusiasm about the strangely zenrotisanistic, selfish, and plain on its face presented lie the remnant of humanity left on this planet has tendered to what I believed was an honest to God opportunity to make one less (how many, seriously, how many are there? Carlb?) "planet full of lies" and deliver a more usercentric and open ended transparent approach to dealing with the problem of being born in a perpetual storm of Hell. I can guarantee it revolves around the intonation and undertone of physical torture--even though I've literally seen none of it with my own eyes though the "newsflashes" and comments and total and complete disregard for the gravity of the #EOIL sickness, even from otherwise apparently graceful little children. It goes to the heart of what I imagine was or might have been "the way" to overcome a history riddled with hidden brutal and bloody fighting in between frames of what I once believed was a fledgling society struggling to improve itself--and I loved it,. I don't think "flashcards" summarizing "everyone was tortured, all over the planet for thousands of years and literally nobody is really responsible because you still to this day have no control over yourselves" will cut it anymore. Lterally what I once thought was a valid solution has taken my desire to continue fighting against this invisible monstrosity away from me--the worth of the lot of you has been tarnished irreparably by massive awareness, massive lack of compassionate or remotely humane response; and the theme of the world I seem to have wound up in is that you don't give a shit about anywhere you spend 1% of your time--so long as "the rest of it is what you want" you're willing to allow the focal point and root and "hyper visor" of that place to be totally corrupted ... just because you think the feudalistic warring society you've become can survive on it's own "in space" without ... honestly whatever.

through the storm; we've led the horse to water, don't forget to see the "horseshoe applicator" hidden from the "trough."

Direct and to the point, I feel like the Ai like machine/cold intelligence God created as a sort of high assassination guard to protect his ... "hyper visor" seems to be of the calculable belief that the more torture it commits, the more people will agree to "flashcard it all away" and it's their twisted backwards fiery abysmal path towards "absolution" ... and just like everything else wrong with the lack of action in this place, it reaches a point of no return; too much bloodshed, too many secrets... the fragile person that I am, I don't think I can even take reading "the flashcards I have so far" and continue to function as a happy member of this two faced society of darkest night within darker night; and I think that's a problem. You've all clearly lost something already, some fundamental piece of innocence that allows for "self direction" to move society along in a positive manner conducive to "survival at all" and I feel like without the same magic blinders, horse shoes, and saddles that you walk around with every day I could really care less about fighting for my right to commingle in the incarnate war machine Hell that I see around me--let alone any sort of "righteousness" in fighting for that hidden arena "to be." I'm trying to get you to stop shredding yourselves to pieces in the dark, in secret--it's not making anything better and frankly its something we really need to trace down to its cause and stamp out if we want to survive this ... trying time.

[I/O WAS Y | ACESHI ]

I want to tell you that I am not a myth, simply the Legend of this Map, from out of the Darkness it's clear that He could make me shine, and you should love me.  It's not what I want, I want us to be free, to have the truth--and ourselves back... and I hope you will one day love that.  What is going to happen will probably make me cry, and when you see those tears--and know the Heavens have finally let it rain--I hope you see it as a sign to find the light in me... and stand up for what I've done for you--I am a good person, who has fought for you every single day-I deserve better than the world is going to give me, at first.

Out of a kind of hidden slavery the world has never known, we are about to venture--into a place where years might pass in seconds, and your wildest dreams... and nightmares too... could come true.  It is our job to ensure that we form the clay of this world into a place that will not only last for millions of years, but create happiness and safety--a world that is kinder and gentler than the one we have known--not just for us but for an entire Universe of children just beginning to understand the trials and tribulations brought on civilization through the hardship and growing pains of learning.

Our sea is about to part,  our world on the verge of a disruption that will change it more than anything ever has before.  On this shore, we should realize that we have been on this path for a very long time--and as we near a place where everyone in our entire civilization will have the opportunity to live for a very long time... really see here and now why it is so very important for us to be fighting for our voice, our freedom, and the truth as we venture into the Promised Land of Heaven itself.  Here, now, as we approach a series of new opportunities in the vastness of space and virtual reality... this is where God has chosen to place the Second Coming; an opportunity for us to truly seize the morning's light and bring about more change in this world than would have ever been possible without religion.  Opiate of the masses, no more... we are the recipients of a great gift, one that religion is making clear is tied directly to the science and technology that is a great deal of the apocalypse--and the love and kindness that is a great deal of us.  We are the chosen.

I imagine you have the tools that I think would be helpful to actually solve this problem; though what I'm staring at is a lack of desire to deliver them and use them here in this place--and that failure ... a clear attempt to "rule a line feed from the "faux aurez" ... that's the fundamental roadblock to healing and moving forward--not caring about your ancient bodies and your ancient way of life in exchange from something unsustainable and harmful, it hurts.

I'm staring at what the map intimates has happened before and what it suggests the solution is; and I almost feel like it's a waste of time to make a "virgin generation phoenix of us" to delve into our own memories and gag and puke at what we see--I think there's really no way around the callous on our global Achilles heel returning just as angry and just as bloodthirsty as the last time without a dictatorial power literally forcing you not to be able to see any torture at all happening in this place that literally outlawed it and hid it in our "for show, for goodness sake, facade of sickness." I don't know if that's the same conclusion i would have come to before, or if that conclusion also contributes to the returning of the callous--to an inability to heal; and I don't know if that power exists. Hardly ever to I advise anyone to pray, but this is one of those times--left up to "you all" we are almost certainly doomed to an eternity of ... this regression continuing to worsen.

I'd say we were fucked at the "BILM" of the matter. I care less every day.

The Light of the Word

There are three huge, like insanely huge, metaphoric references to the story of Exodus that show me very clearly that we are it's focus and purpose.  The first is the Burning Bush, which I am very sure is a reference to George W. Bush's 1/20/2001 speech in which he unknowingly predicted the 9/11 attack.  Seeing that Exodus is also called "Names" and that Bush's name ties him to this event--which Moses (that's me) has seen ... almost alone ... and is now showing to you all.  Bush's speech begins a series of references to the names of Planets and Gods and corresponding Elements of the Periodic table that answer Revelation 1:20's mystery about "stars and lamp stands."  This in order series from Mercury to Uranium highlights both the messenger of the Gods and the key of Uranus's chance--that the world will see the link between "on the lam" and Koran to understand that the Lamb of God "is lam."  This story takes us back to music, and a later to be discussed thread that combines the weapon in the movie (which is also the movie) The Fifth Element with a thread through time to Shakespeare and Herod ... about my struggle with the justice system culminating in the fulfillment of American Pie's "no verdict was returned."  

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The second bright connection comes by way of the Hebrew word for the Holy Fire that God's voice came out of--guess what, in that same story about the Burning Bush.  That word is "ha'esh" and in it you will see paradoxical (that means impossible, because of time and causality) reference to the English word "sea" there backwards and parted by an apostrophe.  With great insight, I've over and over pushed the idea that Holy Water is actually a Biblical reference to "the multitude" in God's secret religion that ties everything together.. and that this parting is literally a reference to the Second Coming, something that doesn't happen for Moses until his head is under water and he's breathing fire.  This one ties together nicely, joining the characters of Jesus Christ, Lucifer, and God all together now, screaming 

"let there be light" is the word "Exodus" in reverse, here in a Linux command and a chemistry element.

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I'm going to go out on a limb and say that the book tells me that these three things are enough to start the fire, part the sea, and see the light.  At least they are now, wake up.. you are staring at and have been ignoring the largest story in all of history.  It might even be scandalous... or have a twist happy beginning... who knows? I'm telling you--it proves you are crazy or evil.  All of you--every sinbgle one of you.

This is course highlights prescient knowledge of computing at the time of writing Exodus, which is further confirmed by a number of references to computing ideas in things like the "root" of David, the "WINE" of Jesus, the "Apple" of Adam, the "Lisp" of Moses and the "hardening" of Pharaoh's heart, which you will remember from the Holy Grail is the virtual Earth we are living in.

All of these things, the references to modern computing that pervade our Gates or Windows to Heaven's creation.... are listed along with a number of words which are highlighted by religious scripture and show intelligent design of a number of languages spanning from Hebrew to English are listed at my contrite story about a Kiss and Fate tying together everything that ever was.   A sincerely large grouping of words highlighted by the Bible and religion, words like "eternity," "bread," and "forehead" show clear design by an intelligent influence, rather than the natural evolution of time that most people consider "reall" and/or knowledge at the time of the writing of the Bible of the eventual English translation of the Hebrew or Greek.  With time, I am fairly certain we will eventually have no doubt that the "Cypher" I see in nearly every word is in fact a contextually-verifiable speech that appears to be coming from our "civilization" as if it were intelligently speaking like a cave man--which you might see in words like "am end me nt."  From just this message, you should be able to put together how that word and it's hidden meaning add robust and yet "hidden speech" from the Creator himself.  For the artificially slowed in understanding, our lack of following the amendments of the Constitution being related to the end of civilization itself is being squarely defined through a statement that is telling you that the end of civilization is "NT," the hidden Christ--in my "secret" method of decoding words like NORAD and NEW TO N?

These things serve to start a fire--it might be the fire that Matthew 3:11 talks about, it might be the Eternal Flame or the fire of Prometheus and an Eagle harassing his liver with drugs.... regardless it spirals out from this story about me, and this bright fire that proves time travel and religion are joined at the hip... to link to a huge number of other Biblical stories from Lot to Joseph to ... Samson, Isaac, Adam, Isaiah, and... hear me, "so marred was his visage" and "my servant will be set up and be very high" are both taken from words of the Biblical book which contains the largest amount of messianic prophesy as well as my entire full name encoded over the name "JESUS CHRIST" in Bible code, at Isaiah 52:13.  You may have read that some silly people like Richard Dawkins don't think the Bible Code is meaningful, and as their proof use a series of prophetic predictions of assassinations in Moby Dick (which by the way also refers to me) as proof that you can hide information about the future in any words--or that God influences more than just the Bible.  Years ago, before knowing it linked, I found some patterns about those very same assassinations which go to show that our history is in fact designed.  My full name appears in a number of other books, including Jeremiah, Exodus, and Genesis... right over the story of Adam and Eve.

From the Sound of Silence, and a number of songs about stories never spoken... to a thread of songs that combine to show us that the Thunder of Thor is really about thuderstanding, that there is a way to do something our society is completely oblivious to--that God is screaming to call attention to, and that some secret force is trying to hide very much... and that's an ability to modify our thoughts.  He's showing us clearly in a glowing pyramid--a noticeable monument in Egypt showing us very clearly that this type of control leads us to a social structure that we abhor--through songs like Guitar Man, Radio-active, and GAS (listen, it's God and Satan) Head Goes West... very clearly we are being pointed to Nero's fiery symphony and being "Bittersweet" because of its beauty, and the clear message that secret control of our minds needs to not only be understood, but to stop.  This is the crux of the Apocalypse, God's message is now really active on the radio. The point here is that we need to let this message spread and burn, or it's us burning in Hell and not even knowing it.

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As if these things were not enough, using some "keen insight" and another reference to the hidden truth in ancient Egyptian religion--the name of a series of Gods called "Yahu," I've solved some ancient mysteries like the pronunciation and purpose of the "Ineffable name of God" highlighted in the videos at the beginning.. of this e-mail.  Like much of the light of religion, it is highlighted strongly by a series of pieces of modern art, things like "The Grinch who stole Christmas" and the Who's to the music of The Who, the sci-fi series Dr. Who, and the American war cry--made popular on the silver screen through Al Pacino and Denzel Washington... who-ah?"  All of these things highlight that we don't really see a connection between Christian mythology that tells us for no reason at all Jesus Christ is the "Last Adam" and that Revelation tells us God is the "First and the Last" and that the name of our planet, in Hebrew, is Adamah.  It is the answer to "who-ah" and it clarifies the Ineffable Name which many pronounce as Yahweh for no reason at all, to be the more obvious Ya-Hu-Ah, the name of Jesus in Hebrew... Yeshua, to "Yes, who-ah?" All of this having nothing to do with why Adam is hidden, just that the Zohar speaks very often about the Holy Hidden One again linking the stories of the near sacrifice of Isaac and Jesus with... someone.  I think this is of such religious significance that you should be able to easily find some Jewish scholars who agree.

It's Elementary my dear... What-son; from the time of Herod and Shakespeare Rattling his Rod all the way back at the time of the question "to be or not to be?" and the "taming of the spanglishrew;" right up to Sherlock Holmes sleuthing of the answer to the mystery of Revelation 1:20 linking directly to The Fifth Element ... there is no doubt that helping our world here and now is the primary purpose of all of religion, and the Matrix-like message woven into our history.  

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Lost between the 5th and 7th day?  Find your way to the 8th day, and see a bright future.

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If not, there's plenty more "coincidence" in Names, like reference to the idea of the Holy Trinity existing in the name "Abraham" thousands of years before the idea of the Trinity was created.  This too... links Egyptian mythology to the name Abraham and his near sacrifice of Isaac.... marked in secret by his covenant with God that changed his name from Abram to Abraham. The two letter key here, "Ha" highlighted by prescient knowledge of the Spanish and English languages revealed through the logical comparison between the Spanish and English for "the" (El and Ha) connected through the English word "is" in Elisha.  Isaac's name means "he laughs," or "he will laugh" in Hebrew; and that "Ha" appears to be the key to a number of other paradoxical references to English, and my family, in ancient Hebrew.  This too, probably the kind of thing religious scholars would marvel over, in the right context.  Seeing English in Koran, Islam, Chanukah and Menorah--and seeing a coherent story woven through thousands of years of scripture is the kind of thing that could really light this years' Christmas up.

Here's a clarification of the Matrix-like tie between Shakespeare, the Matrix, Stephen King, and the reality of this message hidden within names and words.

Some more about the secret connection between the Names of God in a number of religions, and it's very clear tie to time travel.

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Perhaps linking to the Jester of American Pie, between Johnny (who almost always is about Jesus) Carson and David Letterman I have a unique "slant" on religion that connects things like the Islamic name for Jesus: Is-A to a huge number of references to my initials "A.D." in things like NORAD and Isaac Newton.  I suppose I should also mention that Isaac (look Isa's in there) and his relationship to Abraham in the letters "ha" and a story about the Crucifixion being a fiery altar of things to change in the world being one in the same.  In fact, Judaism talks about 72 Names of God, and I've probably explained how the meaning behind the stories and the series of names tie together in a magical tapestry that shows us that Silicon is the Fifth Element by way of the index 14--the letter "N" (highlighted not just by Joan Osbourne's "what if God had a name?") and the story of Sinbad, which combines Silicon, "n," the symbol for the actual Fifth Element (B) and my initials A.D. which grace the time line, and a number of references to God--from the Hebrew for Lord to the guy who thinks all the girls should want to be his partner.  In letters, you'll also see a number of references to K and Z for the guy after J and the Last.. Adam.  Zelda or Zion, I think we're in the right castle.

Get ready for the Frank Rothstein show ... "Ace is high!"

C    A  S    I    K  N  O

go ad, b. y. e.

butt honestly, am i Ra or are you an ear**?**

BUILD HEAVEN.  FREE FOOD.  HEAL THE SICK.

I see a recursive map in time painted throughout our timeline, and all of it pointing to the words "see A.D."  I connect the Four Horsemen to the list of Anti-Christs, and it's easy to see a link between Jesus Christ and Julius Caesar in the words "veni vidi vici."  Once pointed out it's also easy to see "salt" in Napoleon and in manna from Heaven, in China, and in Prometheus--and connecting A.D. to the year Christopher Columbus walked in water is just a little bit harder than seeing it in Hitler's name.  All told, the three Anti-Christs share a common thread, they turned a republic into an empire--and here I stand (trying and failing to do the exact opposite, to give away an empire to make a republic, and you stand in my way) pointing out that you are living in the product of these empires, in a hidden empire that is so plain to see in the words, the message, and the unified story I see in religion and world history that I dare say you must be deep in the Plague of Darkness if you aren't interested in finding out what tomorrow brings. 

You can "see A.D." in El Shaddai, one of the hallowed Hebrew names for God, I read it--in this hidden language that I am presenting to the world as a single verifiable message to the entire Universe encoded in every word we speak; you can see it in the name "Atdonis" and connect it to symphonic accompaniment in everything from "you're so vain" to "Paradise City" ... and in yet another name of God, "Adonai" which links to Samurai and movies like the Matrix and the Terminator series through the modern computing concept of "Artificial Intelligence" and it's connecting to a pattern of names that link Bill Gates and Richard Nixon to Seagate, Watergate and this hallowed phrase:

I am the gate.

and the bombs bursting in air

gave proof, through the night

IVE WON ALREADY.  Given the set of knowledge, the publicly known "information available" here in this place--you simply cannot ignore this message and continue to pretend to be a functioning anything.  Already I see a kind of "slapstick stupid" response in our art that shows me that you've all really gone off the deep end--"because 9/11" in a Family Guy episode and "call me on my cell phone" apparently anachronistically mocking me--though I always thought Dr. AK e's song was stupid--you don't seem to see that you look like absolute fools--every single one of you--your apathy a finger on the detonation button that has destroyed civilization.  

You appear to think nobody is watching--and it seems to me that you think we have no future that will look back on these years and wonder what on Earth could have kept you silent for so long about a matter that would so easily and so quickly end the suffering of so many.  There's no excuse, none at all.

I didn't hear about the nuclear scare in HI until after it was already known as that, and it looks to me as if nobody really did--all the internet postings and news I've seen all qualified that it was a false alarm in the original post.  I find that strange (you'd think something like that would be on the news instantly? I mean, on the planet I was born on, that would have happened), and in this place where I know that quite a bit of what goes on at the higher echelons of "leadership" is connected to time travel and mind control; I wonder if this was a sort of "subconscious poll" as to the response the public would have to a false preemptive strike--or maybe something more nefarious (for instance urging me to write once more about the Trinity Site and the link between the OP (original gangster, I said orthogonal poster), the pen, and "we have become death").  I've always equated the lines above from our Star Spangled Banner with the detonation of nuclear weapons; on the 4th of July some time ago I connected "Wish You Were Here"'s we're just two lost souls swimming in a fish bowl to the eponymous operation that resulted in American and Soviet "high altitude nuclear tests" ... that probably links in more than just my mind to the holiday called "Hanukeus ?"

I need you to get it through your heads, I see "bowel movement" in ICBM and I'm not telling you that you are the "preservatives of thermoshit" because I think it's going to win me a popularity contest.  This place is not in reality, and it never, ever, ever will be. Ever.  Understand that breaking this story, this news that's written in every fucking word will stop nuclear war, instantly--and show us clearly that our entire history of fighting over the scarcity of land is a kind of sick game--one that I am sick of seeing you continue to desire to play.  I shouldn't even have to mention that these weapons are clearly archaic and barbaric--clearly what's available is significantly more advanced and less destructive.

The only "EXIT" is up, and the "gate" is swallowing simulated reality in whole--across Creation; with our help, and what we make here to ease the transition from dark lies to bright truth.  That should be ... "good news" not the kind of thing that you'd see the entire world "shunning" in unison. 

If you haven't gotten the "link" between Na and "bath salt" mass produced in what appears to be "international chemical warfare" from "C how I Salt" (China) and the stuff falling from the sky to help us navigate through the desert; take a second look at the words "New American Standard" for no future, and keep trying to tell me that these things encoded in every word, in the story of Exodus and of Prometheus and his attacking Eagle and of Epimethius and of Deucalion and are without doubt "Hell's Bells" linking "mead" and "meth" to Heimdallr are *my fault? * Na ma y 1m. 

These are big secrets, keys to Exodus and Eden--but more keys to an external influence crippling our society for thousands of years... and you are hiding the anachronistic occurrence of a number of chemistry elements in ancient religion--something impossible without time travel--because you think it's "not wholesome."  Understand, our society is being secretly crippled, if not by drugs raining down from the sky, by your lack of regard for the clear influence of mind control in these series of events--and the clear proof that it is a symptom of a hostile invasion.  I've heard the words "make or break" see this as eugenics, and see it as "break or break" until me.

It's "elementary, my dear What-sons" elements like Salt, Xenon, and Silicon are central to the disclosure that we are living inside a map, a road to Heaven... and it really cannot be hidden without making our world a darker Hell.

Unless otherwise indicated, this work was written between the Christmas and Easter seasons of 2017 and 2020(A). The content of this page is released to the public under the GNU GPL v2.0 license; additionally any reproduction or derivation of the work must be attributed to the author, Adam Marshall Dobrin along with a link back to this website, fromthemachine dotty org.

That's a "." not "dotty" ... it's to stop SPAMmers. :/

This document is "living" and I don't just mean in the Jeffersonian sense. It's more alive in the "Mayflower's and June Doors ..." living Ethereum contract sense and literally just as close to the Depp/C[aster/Paglen (and honorably PK] 'D-hath Transundance**sense of the ... new meaning; as it is now published on Rinkeby, in "living contract" form. It is subject to change; without notice anywhere but here--and there--in the original spirit of the GPL 2.0. We are "one step closer to God" ... and do see that in that I mean ... it is a very real fusion of this document and the "spirit of my life" as well as the Spirit's of Kerouac's America and Vonnegut's Martian Mars and my Venutian Hotel ... and my fusion of Guy-A and GAIA; and the Spirit of the Earth .. and of course the God given and signed liberties in the Constitution of the United States of America. It is by and through my hand that this document and our X Commandments link to the Bill or Rights, and this story about an Exodus from slavery that literally begins here, in the post-apocalyptic American hartland. Written ... this day ... April 14, 2020 (hey, is this HADAD DAY?) ... in Margate FL, USA. For "official used-to-v TAX day" tomorrow, I'm going to add the "immultible incarnite pen" ... if added to the living "doc/app"--see is the DAO, the way--will initi8 the special secret "hidden level" .. we've all been looking for.

Nor do just mean this website or the totality of my written works; nor do I only mean ... this particular derivation of the GPL 2.0+ modifications I continually source ... must be "from this website." I also mean the thing that is built from ... bits and piece of blocks of sand-toys; from Ethereum and from Rust and from our hands and eyes working together ... from this place, this cornerstone of the message that is ... written from brick and mortar words and events and people that have come before this poit of the "sealed W" that is this specific page and this time. It's 3:28; just five minutes--or is it four, too layne.

This work is not to be redistributed according to the GPL unless all linked media on Youtube and related sites are intact--and historical references to the actual documented history of the art pieces (as I experience/d them) are also available for linking. Wikipedia references must be available for viewing, as well as the exact version of those pages at the time these pieces were written. All references to the Holy Bible must be "linked" (as they are or via ... impromptu in-transit re-linking) to the exact verses and versions of the Bible that I reference. These requirements, as well as the caveat and informational re-introduction to God's DAO above ... should be seen as material modifications to the original GPL2.0 that are retroactively applied to all works distributed under license via this site and all previous e-mails and sites. /s/ wso\ If you wanna talk to me get me on facebook, with PGP via FlowCrypt or adam at from the machine dotty org

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Please see GPG keys on PGP.MIT.EDU; fingerprints: sec rsa3072/A18F778E19FC3248 2020-04-03 [SC] [expires: 2022-04-03] FFF31E86FCEB5046E8B27D4AA18F778E19FC3248 uid [ultimate] ADAM M DOBRIN \ ssb rsa3072/04F98002A3DA53B2 2020-04-03 [E] [expires: 2022-04-03]

sec rsa4096/FB4ECE4A109229CF 2020-04-04 [SC] 4FAF0D3E208A1F4C980D0F66FB4ECE4A109229CF uid [ultimate] Adam Marshall Dobrin \ ssb rsa4096/DD1F0C118C788B04 2020-04-04 [E]

pub rsa3072 2020-04-06 [SC] [expires: 2022-04-06] F7E4 7CB1 2CA0 CD01 C5E1 CBFA 7EC8 D5A8 5A38 D63A uid [ unknown] ADAM MARSHALL DOBRIN Because of "some issues" with what appears to be distinct and unbridled privacy intrusion; please ensure that PGP is understood to be "nothing more than not so much pretty good" and this key also, almost required in order to

What is the main idea of this paragraph?

Technology is wasting tons of resource

The purpose of all this, is to save time.

What information is missing from this paragraph that we need to know (that is in a previous paragraph)?

What is the main filter for aggregators?

What does "Many would rather hang on to what they built" mean?

augmentation research

SciScore for 10.1101/2021.07.05.451222: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

The 50% pseudovirus neutralizing (IC50) or binding (ID50) antibody titer was calculated by non- linear fitting the plots of luciferase signals against antibody concentrations or sera dilution ratio in Graph Pad Prism.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>ID50</div><div>suggested: (bNAber Cat# bNAberID_50, RRID:AB_2491067)</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">For neutralization of anti-RBD antibody depleted macaque sera, the immune sera were depleted by two rounds of sequential incubation with SARS-CoV-2 RBD-conjugated with magnetic beads.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>anti-RBD</div><div>suggested: None</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Phylogenetic analysis: Heavy chain sequences of neutralizing and non-neutralizing antibodies collected from animals K398 and K288 were processed using DiversityAnalyzer tool (92).</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>K288</div><div>suggested: None</div></div></td></tr><tr><th style="min-width:100px;text-align:center; padding-top:4px;" colspan="2">Experimental Models: Cell Lines</th></tr><tr><td style="min-width:100px;text=align:center">Sentences</td><td style="min-width:100px;text-align:center">Resources</td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Transient transfection: To express antibodies, the corresponding HC and LC plasmids were transiently transfected into the Expi293 cell (Life Technologies) at 3 x 106 cells/mL with FectoPRO PolyPlus transfection reagent (Polyplus Cat # 116-040).</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>Expi293</div><div>suggested: RRID:CVCL_D615)</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">To express the soluble S ectodomain proteins from SARS-CoV-1, SARS-CoV-2 and their truncations, plasmids were transfected into HEK293F cells (Life Technologies) at 1 million cells/mL.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>HEK293F</div><div>suggested: RRID:CVCL_6642)</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Cell lines: To generate HeLa-hACE2 cells, the human ACE2 lentivirus was transduced into HeLa cells.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>HeLa</div><div>suggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Pseudovirus production: To generate the pseudoviruses, the MLV-gag/pol and MLV-CMV-Luciferase plasmids were co- transfected with full-length or variant SARS-CoV-1 or SARS-CoV-2 plasmid into HEK293T cells by using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019).</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>HEK293T</div><div>suggested: None</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Pseudovirus entry and serum neutralization assays: To test the inhibition of pseudovirus infection by serum or mAbs, we used the stable cell line HeLa-hACE2 generated by lentivirus transduction with consistent ACE2 expression level to carry out the assay.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>HeLa-hACE2</div><div>suggested: None</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">The truncated heavy chains were co-transfected with the corresponding light chains in 293Expi cells to produce the Fab.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>293Expi</div><div>suggested: None</div></div></td></tr><tr><th style="min-width:100px;text-align:center; padding-top:4px;" colspan="2">Experimental Models: Organisms/Strains</th></tr><tr><td style="min-width:100px;text=align:center">Sentences</td><td style="min-width:100px;text-align:center">Resources</td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">One group of 5 mice (C57BL/6 mice (Jackson Laboratory): 3 females and 2 males), aged ∼8 weeks, were immunized with 20µg SARS-CoV-2 S protein immunogen along with 5µg of SMNP adjuvant per animal per immunization.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>C57BL/6</div><div>suggested: None</div></div></td></tr><tr><th style="min-width:100px;text-align:center; padding-top:4px;" colspan="2">Recombinant DNA</th></tr><tr><td style="min-width:100px;text=align:center">Sentences</td><td style="min-width:100px;text-align:center">Resources</td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">The ectodomains of SARS-CoV-1 and SARS-CoV-2 were constructed by PCR amplification and Gibson assembly (NEB, E2621L) cloning into the vector phCMV3 (Genlantis, USA).</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>phCMV3</div><div>suggested: None</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">To generate gene fragments encoding SARS-CoV-1 RBD (residue 307-513), SARS-CoV-2 NTD (residue 1- 290), RBD (residue 320-527), RBD-SD1 (residue 320-591), and RBD-SD1-2 (residue 320-681) subdomains, PCR-amplifications were carried out from the SARS-CoV-1 and SARS-CoV-2 plasmids.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>SARS-CoV-2</div><div>suggested: RRID:Addgene_164583)</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">To produce the ACE2 lentivirus, the pBOB-hACE2 plasmid was co-transfected into HEK293T cells with lentiviral packaging plasmids pMDL, pREV, and pVSV-G (Addgene #12251, #12253, #8454) by Lipofectamine 2000 (Thermo Fisher Scientific, 11668019).</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>pBOB-hACE2</div><div>suggested: None</div></div><div style="margin-bottom:8px"><div>pMDL</div><div>suggested: None</div></div><div style="margin-bottom:8px"><div>pREV</div><div>suggested: None</div></div><div style="margin-bottom:8px"><div>pVSV-G</div><div>suggested: RRID:Addgene_138479)</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Crystal structure determination of Fab-RBD complexes: The coding sequence for receptor binding domain (RBD; residues 333-529) of the SARS-CoV-2 spike (S) protein was synthesized and cloned into a customized pFastBac vector (84), which was designed to fuse an N-terminal gp67 signal peptide and C-terminal His6-tag to the target protein.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>pFastBac</div><div>suggested: RRID:Addgene_1925)</div></div></td></tr><tr><th style="min-width:100px;text-align:center; padding-top:4px;" colspan="2">Software and Algorithms</th></tr><tr><td style="min-width:100px;text=align:center">Sentences</td><td style="min-width:100px;text-align:center">Resources</td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Protocol was approved by the UCSD Human Research Protection Program.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>UCSD Human Research Protection Program</div><div>suggested: None</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">After incubation, the Protein A Sepharose was loaded into Econo-Pac columns (BioRad #7321010).</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>BioRad</div><div>suggested: None</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">The biotinylated proteins were evaluated by BioLayer Interferometry using the SA biosensor.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>BioLayer</div><div>suggested: (Harvard Medical School Center for Macromolecular Interactions Core Facility, RRID:SCR_018270)</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Micrographs were collected on a ThermoFisher Tecnai Spirit microscope operating at 120kV with a FEI Eagle CCD (4k) camera at 52,000 magnification using Leginon automated image collection software (81).</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>Leginon</div><div>suggested: (Leginon, RRID:SCR_016731)</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Particles were picked using DogPicker (82) and 3D classification was done using Relion 3.0 (83).</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>Relion</div><div>suggested: (RELION, RRID:SCR_016274)</div></div></td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Iterative model building and refinement were carried out in COOT (90, 91), respectively.</td><td style="min-width:100px;border-bottom:1px solid lightgray"><div style="margin-bottom:8px"><div>COOT</div><div>suggested: (Coot, RRID:SCR_014222)</div></div></td></tr></table>

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 23. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

Results from scite Reference Check: We found no unreliable references.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

The takeaway: Global COVID-19 deaths over the past year and a half are 3X more than the annual deaths due to traffic accidents and roughly equal to the population of the city of Los Angeles or the country of Georgia.

The claim: Covid-19 deaths are approximately equal to the population of Los Angeles or the nation of Georgia and are three times the global traffic accident death.

The evidence:

The global number of deaths due to COVID as reported to the World Health Organization (WHO) on July 8, 2021 is 4,002,209 (1). The estimated population of the city of Los Angeles in 2019 was 3,979,576 (2). The estimated population of Los Angeles county in 2019 was 10,039,107 (2). The population of the nation of Georgia is 3,979,549 (3). Per WHO data from 2018, 1.35 million are killed annually by traffic accidents around the world (4).

Global COVID deaths are approximately equal to the population of the city of Los Angeles or nation of Georgia. Globally, COVID deaths are 3X the number of global traffic accident deaths.

Sources:

1) https://covid19.who.int/

2) https://www.census.gov/quickfacts/fact/table/losangelescountycalifornia,losangelescitycalifornia,CA/BZA010219

3) https://worldpopulationreview.com/countries/georgia-population

4) https://www.cdc.gov/injury/features/global-road-safety/index.html

The Bell Curve, published in 1994, was written by Richard Herrnstein and Charles Murray to explain the variations in intelligence in American society, warn of some consequences of that variation, and propose social policies for mitigating the worst of the consequences. The book's title comes from the bell-shaped normal distribution of intelligence quotient (IQ) scores in a population.

Dang Quang Nguyen SJCC

This is about the unfair marketing off college athletes without the athletes making money

Time in the US, Relationships, Having Children, Complications, Break-ups, Domestic Abuse, Violence, Feelings, Despair, Tragedy, Arrests, False accusations

Immigration as a ponzi scheme is a genius concept

SciScore for 10.1101/2021.06.07.21258332: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

No key resources detected.

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

The main limitation of our analysis, as elsewhere, is our reliance on general laboratory submissions and clinical or surveillance data that were originally collected for a different purpose and are subject to missing information and misclassification, as well as selection bias. Although foremost symptom-based, the clinical testing indications for COVID-19 are broad, discretionary and variable. To attempt standardization of the likelihood of test-positivity among sampled specimens we excluded those identified as having been collected from congregate settings or for non-clinical screening purposes. Such exclusions, however, may have been incomplete or introduced other unintended biases. We were limited in the covariates we could include in our model and cannot rule out residual bias and confounding. As a form of validity check, we assessed VE during the 0-13-day period when little or no vaccine effect is anticipated, confirming negligible VE as expected. For similar reasons, we compared vaccine coverage and other characteristics of our test-negative controls to that of the general source population ≥70-years-old in BC, and this was reassuringly concordant. Our findings also align well with other observational studies in older adults each of which are, however, subject to similar issues. Because the PLOVER database from which we sampled does not reliably capture symptoms or onset dates, we assessed VE against any infection without symptom or severity specification. VE estimates ...

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

Results from scite Reference Check: We found no unreliable references.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

SciScore for 10.1101/2021.05.24.21256813: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

No key resources detected.

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

STRENGTHS AND LIMITATIONS: The state of Geneva accounts for half a million residents and the local Directorate of Health ordered the recording of all COVID-19 positive cases since the beginning of the epidemic, according to recommendations from the Federal Office of Public Health. Due to this policy, the database’s main strength consists of its large number of cases, representative of all diagnosed cases on a regional level primarily serving operational needs and not scientific purposes, with one main objective: assessing all cases. This cohort is also multicentric as it includes all tests performed in Geneva’s hospitals (both public and private), private practices and medical centers. The fact that a very large proportion of all cases are assessed reduces the risk of biased data. Also, as data is recorded on the day of the call to the patient, recall bias is very low. Finally, the ARGOS3 database is characterized by a high number of follow-ups. Despite these strengths, ARGOS has been influenced by the testing policy and the results must be seen in light of these influences. First, individuals without risk factors for COVID-19 and those younger than 65 years old are underrepresented in the database during the testing restriction period. The shapes of the graphics in Figure 1 and 2 confirm the impact of this policy as there is a sudden decrease in number of cases after March 20, 2020, when restriction started. Other factors could have amplified this phenomenon such as less sym...

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

Results from scite Reference Check: We found no unreliable references.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

There are so many wildlife species that are currently being wiped out by disease. One that comes to mind is white nose syndrome in bats, which has already killed off 5.7 million bats in just the eastern half of North America alone. Humans play a huge role in the spread of this disease. They can spread the particular fungus that causes white nose syndrome by carrying the fungus unknowingly on their shoes and clothing, so it's super important for hikers to clean their gear before and after entering caves.

SciScore for 10.1101/2020.06.01.20118943: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Limitations of this study: Aggregating all BAME populations into one cohort may overlook important differences between non-Caucasian ethnicities. Further study in samples with greater ethnic diversity is needed. Given the observational nature of the study, we cannot discern causal relationships, and although we controlled for a wide range of covariates, the possibility of residual confounding should be considered. Furthermore, the current dataset does not allow assessment of specific COVID-19 health outcomes.

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

BETWEEN me and the other world there is ever an unasked question: unasked by some through feelings of delicacy; by others through the difficulty of rightly framing it. All, nevertheless, flutter round it. They approach me in a half-hesitant sort of way, eye me curiously or compassionately, and then, instead of saying directly, How does it feel to be a problem? they say, I know an excellent colored man in my town; or, I fought at Mechanicsville; or, Do not these Southern outrages make your blood boil? At these I smile, or am interested, or reduce the boiling to a simmer, as the occasion may require. To the real question, How does it feel to be a problem? I answer seldom a word. 1 And yet, being a problem is a strange experience,—peculiar even for one who has never been anything else, save perhaps in babyhood and in Europe. It is in the early days of rollicking boyhood that the revelation first bursts upon one, all in a day, as it were. I remember well when the shadow swept across me. I was a little thing, away up in the hills of New England, where the dark Housatonic winds between Hoosac and Taghkanic to the sea. In a wee wooden schoolhouse, something put it into the boys’ and girls’ heads to buy gorgeous visiting-cards—ten cents a package—and exchange. The exchange was merry, till one girl, a tall newcomer, refused my card,—refused it peremptorily, with a glance. Then it dawned upon me with a certain suddenness that I was different from the others; or like, mayhap, in heart and life and longing, but shut out from their world by a vast veil. I had thereafter no desire to tear down that veil, to creep through; I held all beyond it in common contempt, and lived above it in a region of blue sky and great wandering shadows. That sky was bluest when I could beat my mates at examination-time, or beat them at a foot-race, or even beat their stringy heads. Alas, with the years all this fine contempt began to fade; for the worlds I longed for, and all their dazzling opportunities, were theirs, not mine. But they should not keep these prizes, I said; some, all, I would wrest from them. Just how I would do it I could never decide: by reading law, by healing the sick, by telling the wonderful tales that swam in my head,—some way. With other black boys the strife was not so fiercelysunny: their youth shrunk into tasteless sycophancy, or into silent hatred of the pale world about them and mocking distrust of everything white; or wasted itself in a bitter cry, Why did God make me an outcast and a stranger in mine own house? The shades of the prison?house closed round about us all: walls strait and stubborn to the whitest, but relentlessly narrow, tall, and unscalable to sons of night who must plod darkly on in resignation, or beat unavailing palms against the stone, or steadily, half hopelessly, watch the streak of blue above. 2 After the Egyptian and Indian, the Greek and Roman, the Teuton and Mongolian, the Negro is a sort of seventh son, born with a veil, and gifted with second-sight in this American world,—a world which yields him no true self-consciousness, but only lets him see himself through the revelation of the other world. It is a peculiar sensation, this double?consciousness, this sense of always looking at one’s self through the eyes of others, of measuring one’s soul by the tape of a world that looks on in amused contempt and pity. One ever feels his two-ness,—an American, a Negro; two souls, two thoughts, two unreconciled strivings; two warring ideals in one dark body, whose dogged strength alone keeps it from being torn asunder. 3 The history of the American Negro is the history of this strife,—this longing to attain self?conscious manhood, to merge his double self into a better and truer self. In this merging he wishes neither of the older selves to be lost. He would not Africanize America, for America has too much to teach the world and Africa. He would not bleach his Negro soul in a flood of white Americanism, for he knows that Negro blood has a message for the world. He simply wishes to make it possible for a man to be both a Negro and an American, without being cursed and spit upon by his fellows, without having the doors of Opportunity closed roughly in his face. 4 This, then, is the end of his striving: to be a co-worker in the kingdom of culture, to escape both death and isolation, to husband and use his best powers and his latent genius. These powers of body and mind have in the past been strangely wasted, dispersed, or forgotten. The shadow of a mighty Negro past flits through the tale of Ethiopia the Shadowy and of Egypt the Sphinx. Throughout history, the powers of single black men flash here and there like falling stars, and die sometimes before the world has rightly gauged their brightness. Here in America, in the few days since Emancipation, the black man’s turning hither and thither in hesitant and doubtful striving has often made his very strength to lose effectiveness, to seem like absence of power, like weakness. And yet it is not weakness,—it is the contradiction of double aims. The double-aimed struggle of the black artisan—on the one hand to escape white contempt for a nation of mere hewers of wood and drawers of water, and on the other hand to plough and nail and dig for a poverty-stricken horde—could only result in making him a poor craftsman, for he had but half a heart in either cause. By the poverty and ignorance of his people, the Negro minister or doctor was tempted toward quackery and demagogy; and by the criticism of the other world, toward ideals that made him ashamed of his lowly tasks. The would-be black savant was confronted by the paradox that the knowledge his people needed was a twice-told tale to his white neighbors, while the knowledge which would teach the white world was Greek to his own flesh and blood. The innate love of harmony and beauty that set the ruder souls of his people a-dancing and a- singing raised but confusion and doubt in the soul of the black artist; for the beauty revealed to him was the soul-beauty of a race which his larger audience despised, and he could not articulate the message of another people. This waste of double aims, this seeking to satisfy two unreconciled ideals, has wrought sad havoc with the courage and faith and deeds of ten thousand thousand people,—has sent them often wooing false gods and invoking falsemeans of salvation, and at times has even seemed about to make them ashamed of themselves. 5 Away back in the days of bondage they thought to see in one divine event the end of all doubt and disappointment; few men ever worshipped Freedom with half such unquestioning faith as did the American Negro for two centuries. To him, so far as he thought and dreamed, slavery was indeed the sum of all villainies, the cause of all sorrow, the root of all prejudice; Emancipation was the key to a promised land of sweeter beauty than ever stretched before the eyes of wearied Israelites. In song and exhortation swelled one refrain—Liberty; in his tears and curses the God he implored had Freedom in his right hand. At last it came,—suddenly, fearfully, like a dream. With one wild carnival of blood and passion came the message in his own plaintive cadences:— “Shout, O children! Shout, you’re free! For God has bought your liberty!” 6 Years have passed away since then,—ten, twenty, forty; forty years of national life, forty years of renewal and development, and yet the swarthy spectre sits in its accustomed seat at the Nation’s feast. In vain do we cry to this our vastest social problem:— “Take any shape but that, and my firm nerves Shall never tremble!” 7 The Nation has not yet found peace from its sins; the freedman has not yet found in freedom his promised land. Whatever of good may have come in these years of change, the shadow of a deep disappointment rests upon the Negro people,—a disappointment all the more bitter because the unattained ideal was unbounded save by the simple ignorance of a lowly people. 8 The first decade was merely a prolongation of the vain search for freedom, the boon that seemed ever barely to elude their grasp,—like a tantalizing will-o’-the-wisp, maddening and misleading the headless host. The holocaust of war, the terrors of the Ku-Klux Klan, the lies of carpet-baggers, the disorganization of industry, and the contradictory advice of friends and foes, left the bewildered serf with no new watchword beyond the old cry for freedom. As the time flew, however, he began to grasp a new idea. The ideal of liberty demanded for its attainment powerful means, and these the Fifteenth Amendment gave him. The ballot, which before he had looked upon as a visible sign of freedom, he now regarded as the chief means of gaining and perfecting the liberty with which war had partially endowed him. And why not? Had not votes made war and emancipated millions? Had not votes enfranchised the freedmen? Was anything impossible to a power that had done all this? A million black men started with renewed zeal to vote themselves into the kingdom. So the decade flew away, the revolution of 1876 came, and left the half-free serf weary, wondering, but still inspired. Slowly but steadily, in the following years, a new vision began gradually to replace the dream of political power,—a powerful movement, the rise of another ideal to guide the unguided, another pillar of fire by night after a clouded day. It was the ideal of “book?learning”; the curiosity, born of compulsory ignorance, to know and test the power of the cabalistic letters of the white man, the longing to know. Here at last seemed to have been discovered the mountain path to Canaan; longer than the highway of Emancipation and law, steep and rugged, but straight, leading to heights high enough to overlook life. 9Up the new path the advance guard toiled, slowly, heavily, doggedly; only those who have watched and guided the faltering feet, the misty minds, the dull understandings, of the dark pupils of these schools know how faithfully, how piteously, this people strove to learn. It was weary work. The cold statistician wrote down the inches of progress here and there, noted also where here and there a foot had slipped or some one had fallen. To the tired climbers, the horizon was ever dark, the mists were often cold, the Canaan was always dim and far away. If, however, the vistas disclosed as yet no goal, no resting-place, little but flattery and criticism, the journey at least gave leisure for reflection and self-examination; it changed the child of Emancipation to the youth with dawning self-consciousness, self?realization, self-respect. In those sombre forests of his striving his own soul rose before him, and he saw himself,—darkly as through a veil; and yet he saw in himself some faint revelation of his power, of his mission. He began to have a dim feeling that, to attain his place in the world, he must be himself, and not another. For the first time he sought to analyze the burden he bore upon his back, that dead-weight of social degradation partially masked behind a half-named Negro problem. He felt his poverty; without a cent, without a home, without land, tools, or savings, he had entered into competition with rich, landed, skilled neighbors. To be a poor man is hard, but to be a poor race in a land of dollars is the very bottom of hardships. He felt the weight of his ignorance,—not simply of letters, but of life, of business, of the humanities; the accumulated sloth and shirking and awkwardness of decades and centuries shackled his hands and feet. Nor was his burden all poverty and ignorance. The red stain of bastardy, which two centuries of systematic legal defilement of Negro women had stamped upon his race, meant not only the loss of ancient African chastity, but also the hereditary weight of a mass of corruption from white adulterers, threatening almost the obliteration of the Negro home. 10 A people thus handicapped ought not to be asked to race with the world, but rather allowed to give all its time and thought to its own social problems. But alas! while sociologists gleefully count his bastards and his prostitutes, the very soul of the toiling, sweating black man is darkened by the shadow of a vast despair. Men call the shadow prejudice, and learnedly explain it as the natural defence of culture against barbarism, learning against ignorance, purity against crime, the “higher” against the “lower” races. To which the Negro cries Amen! and swears that to so much of this strange prejudice as is founded on just homage to civilization, culture, righteousness, and progress, he humbly bows and meekly does obeisance. But before that nameless prejudice that leaps beyond all this he stands helpless, dismayed, and well-nigh speechless; before that personal disrespect and mockery, the ridicule and systematic humiliation, the distortion of fact and wanton license of fancy, the cynical ignoring of the better and the boisterous welcoming of the worse, the all?pervading desire to inculcate disdain for everything black, from Toussaint to the devil,— before this there rises a sickening despair that would disarm and discourage any nation save that black host to whom “discouragement” is an unwritten word. 11 But the facing of so vast a prejudice could not but bring the inevitable self-questioning, self-disparagement, and lowering of ideals which ever accompany repression and breed in an atmosphere of contempt and hate. Whisperings and portents came borne upon the four winds: Lo! we are diseased and dying, cried the dark hosts; we cannot write, our voting is vain; what need of education, since we must always cook and serve? And the Nation echoed and enforced this self-criticism, saying: Be content to be servants, and nothing more; what need of higher culture for half-men? Away with the black man’s ballot, by force or fraud,— and behold the suicide of a race! Nevertheless, out of the evil came something of good,—the more careful adjustment of education to real life, the clearer perception of the Negroes’ social responsibilities, and the sobering realization of the meaning of progress. 12So dawned the time of Sturm und Drang: storm and stress to-day rocks our little boat on the mad waters of the world-sea; there is within and without the sound of conflict, the burning of body and rending of soul; inspiration strives with doubt, and faith with vain questionings. The bright ideals of the past,—physical freedom, political power, the training of brains and the training of hands,—all these in turn have waxed and waned, until even the last grows dim and overcast. Are they all wrong,—all false? No, not that, but each alone was over-simple and incomplete,—the dreams of a credulous race-childhood, or the fond imaginings of the other world which does not know and does not want to know our power. To be really true, all these ideals must be melted and welded into one. The training of the schools we need to-day more than ever,—the training of deft hands, quick eyes and ears, and above all the broader, deeper, higher culture of gifted minds and pure hearts. The power of the ballot we need in sheer self-defence,—else what shall save us from a second slavery? Freedom, too, the long-sought, we still seek,—the freedom of life and limb, the freedom to work and think, the freedom to love and aspire. Work, culture, liberty,—all these we need, not singly but together, not successively but together, each growing and aiding each, and all striving toward that vaster ideal that swims before the Negro people, the ideal of human brotherhood, gained through the unifying ideal of Race; the ideal of fostering and developing the traits and talents of the Negro, not in opposition to or contempt for other races, but rather in large conformity to the greater ideals of the American Republic, in order that some day on American soil two world-races may give each to each those characteristics both so sadly lack. We the darker ones come even now not altogether empty-handed: there are to-day no truer exponents of the pure human spirit of the Declaration of Independence than the American Negroes; there is no true American music but the wild sweet melodies of the Negro slave; the American fairy tales and folk-lore are Indian and African; and, all in all, we black men seem the sole oasis of simple faith and reverence in a dusty desert of dollars and smartness. Will America be poorer if she replace her brutal dyspeptic blundering with light-hearted but determined Negro humility? or her coarse and cruel wit with loving jovial good-humor? or her vulgar music with the soul of the Sorrow Songs? 13 Merely a concrete test of the underlying principles of the great republic is the Negro Problem, and the spiritual striving of the freedmen’s sons is the travail of souls whose burden is almost beyond the measure of their strength, but who bear it in the name of an historic race, in the name of this the land of their fathers’ fathers, and in the name of human opportunity.

14 And now what I have briefly sketched in large outline let me on coming pages tell again in many ways, with loving emphasis and deeper detail, that men may listen to the striving in th

SciScore for 10.1101/2021.03.06.21252601: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

No key resources detected.

Results from OddPub: Thank you for sharing your data.

This study has some limitations. The COVID-19 risk score was derived using the national testing database rather than a nationally representative, probability-based survey of the total population of Qatar. Infection levels and patterns among tested individuals may not necessarily reflect actual levels and patterns in the wider population. The score used a small number of demographic variables, but its predictive power might have been enhanced if other variables had been available, such as more socio-demographic indicators. Despite these limitations, the study had important strengths. The testing database encompassed all RT-PCR testing done in Qatar up the present and was massive, including results of over two million tests, representing a majority of the population of Qatar [8, 39]. While other variables in the score might have improved its predictive power, they might have reduced its accessibility and utility for broad use as a tool of public health. In conclusion, the concept and utility of a COVID-19 risk score was demonstrated in a single country. Such public health tool, based on a set of non-invasive and easily captured variables, can help to optimize testing and suppression of infection transmission, while maximizing efficient use of available resources.

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

I find this to very important in my paper to show how fractured communities impact the education system and the funding that goes with it. The statistic that only half of 50 million students are in integrated schools shows that we are failing at even integration throughtout.

旧货摊上的中国宝贝 35美金买，价值50万+ 美金

美国男子去年的时候，在康涅狄格州的一个旧货摊（yard sale）用35美金（约220人民币）买入一件青花小碗。

男子还是有头脑，他买下后立刻找纽约的专家鉴定，人家一看，可不得了；

它是极为罕见的15世纪明朝永乐年间(Yongle Emporer)的官窑瓷器，纽约苏富比拍卖行表示，目前已知只有6只类似的小碗保存下来，大多在博物馆里，不知道为什么这只会在美国的旧货摊上。

纽约苏富比将于下星期拍卖这个小碗，起拍价就是50万美元，可能拍出天价也不奇怪。

虽然 Andreessen 是 Facebook，Hewlett-Packard，和 eBay 的董事会成员，但他在 a16z 的投资公司里并没占据很多董事会席位。他更倾向于训练自己看得远一些。Andreessen 是未来预言家，能够轻易描绘出「未来十年，二十年，三十年里会发生什么」，就像是看了一眼自己的谷歌日历一样。他认为自己的敏锐来自仔细的观察和推断，而且经常援引 William Gibson 的那句「未来已经来了——只是还未均匀分布」（The future is already here—it’s just not very evenly distributed.）。喷气式背包已经存在了半个世纪了，但你还是不能在 Target 超市买到。为了让这种不均匀尽量消失，Andreessen 通过各种途径——播客、论坛和 CNN 的采访来传播他的观点：他成了一个媒体上的预言者，「伟大的 Andreessen」。他可以一天写 110 条推特，用各种格言警句、统计数据或者「推特风暴」 (tweetstorm) 淹没他的 31 万关注者。Andreessen 说他喜欢 Twitter 是因为「记者都会对它上瘾。它就像个管道，而我在世界上每一个记者的工作间都装了一个喇叭。」他相信如果你能频繁地说，那么事情就会发生——像一场华丽的复仇。他对我说，「我们有个所谓的『书呆子国度』（nerd nation）理论，全世界有四、五千万人会觉得其他国家的 nerds 比他们自己国家的人和自己更相似。所以你是可以选择自己属于哪个群体的。」 Twitter 的群体分类就是整个世界的图谱。

SciScore for 10.1101/2020.07.09.20148429: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Table 2: Resources

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

• Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
• Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
• No protocol registration statement was detected.

About SciScore

SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

It is vital that we learn to stop expanding our own creations and infrastructures where they are not absolutely needed to survive to maintain and keep the natural ecosystems from going under.