Functional study methods:

WAVE DNA fragment analysis to screen for variant

VWF collagen binding activity

cone and plate analysis (CPA)

Microfluidics

static VWF-platelet receptor binding assay

protein expression in cultured HEK293 cell clones

Near and far-UV circular dichroism spectra

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Trevor Bedford. (2022, January 28). Omicron viruses can be divided into two major groups, referred to as PANGO lineages BA.1 and BA.2 or @nextstrain clades 21K and 21L. The vast majority of globally sequenced Omicron have been 21K (~630k) compared a small minority of 21L (~18k), but 21L is gaining ground. 1/15 [Tweet]. @trvrb. https://twitter.com/trvrb/status/1487105396879679488

Carreño, Juan Manuel, Hala Alshammary, Johnstone Tcheou, Gagandeep Singh, Ariel Raskin, Hisaaki Kawabata, Levy Sominsky, et al. ‘Activity of Convalescent and Vaccine Serum against SARS-CoV-2 Omicron’. Nature, 31 December 2021, 1–8. https://doi.org/10.1038/s41586-022-04399-5.

N, P, & R. (2021, November 27). Does omicron pose a risk to the vaccinated? Too early to tell, epidemiologist says. NPR. https://www.npr.org/2021/11/27/1059539509/too-early-to-tell-whether-omicron-poses-a-threat-to-vaccinated-epidemiologist-sa

Keerthivasan, S., Şenbabaoğlu, Y., Martinez-Martin, N., Husain, B., Verschueren, E., Wong, A., Yang, Y. A., Sun, Y., Pham, V., Hinkle, T., Oei, Y., Madireddi, S., Corpuz, R., Tam, L., Carlisle, S., Roose-Girma, M., Modrusan, Z., Ye, Z., Koerber, J. T., & Turley, S. J. (2021). Homeostatic functions of monocytes and interstitial lung macrophages are regulated via collagen domain-binding receptor LAIR1. Immunity, 54(7), 1511-1526.e8. https://doi.org/10.1016/j.immuni.2021.06.012

Another open-label trial NCT02735707 showed that both IL-6 receptor antagonists improved recovery and survival compared to standard care https://doi.org/10.1101/2021.01.07.21249390 (preprint)

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How scientists know COVID-19 is way deadlier than the flu. (2020, July 2). Science. https://www.nationalgeographic.com/science/2020/07/coronavirus-deadlier-than-many-believed-infection-fatality-rate-cvd/

Starr, T. N., Greaney, A. J., Hilton, S. K., Crawford, K. H., Navarro, M. J., Bowen, J. E., Tortorici, M. A., Walls, A. C., Veesler, D., & Bloom, J. D. (2020). Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding [Preprint]. Microbiology. https://doi.org/10.1101/2020.06.17.157982

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This is good for my purposes. It means high doses can be taken for brain effects without disturbing stomach acidity. The question remains, of course, whether coinciding CNS tolerance develops.

H1 antihistamines enhance the opioid high in humans. Hospitals sometimes administer antihistamines in combination with opioids. It's not hard to find people online who are using this combination recreationally.

This suggests that a beta 2 agonist may be appropriate for sleep.

Note: they appear to be talking about the number of dreams recalled (due to awakenings) rather than the actual number of dreams.

Surprisingly, beta-blockers, unlike alpha-blockers, appear to impair sleep.

Surprisingly, beta-blockers, unlike alpha-blockers, appear detrimental to sleep. I would speculate that this could be the result of a shift in autonomic tone, similar to how caffeine tends to lower heart rate.