10,000 Matching Annotations
  1. Feb 2022
  2. elifesciences.org elifesciences.org
    The human coronavirus HCoV-229E S-protein structure and receptor binding
    4
    3. jackiekrauss 21 Feb 2022
      The coronavirus S-protein mediates receptor binding and fusion of the viral and host cell membranes. In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation but how S-protein function is maintained is not understood. Reported are the X-ray crystal structures of Class III-V RBDs in complex with human aminopeptidase N (hAPN), as well as the electron cryomicroscopy structure of the 229E S-protein. The structures show that common core interactions define the specificity for hAPN and that the peripheral RBD sequence variation is accommodated by loop plasticity. The results provide insight into immune evasion and the cross-species transmission of 229E and related coronaviruses. We also find that the 229E S-protein can expose a portion of its helical core to solvent. This is undoubtedly facilitated by hydrophilic subunit interfaces that we show are conserved among coronaviruses. These interfaces likely play a role in the S-protein conformational changes associated with membrane fusion.
      ann:summary
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    elifesciences.org/articles/51230
  3. elifesciences.org elifesciences.org
    Dual roles for ATP in the regulation of phase separated protein aggregates in Xenopus oocyte nucleoli
    8
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    elifesciences.org/articles/35224
  4. elifesciences.org elifesciences.org
    Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis
    6
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    elifesciences.org/articles/02996
  5. Jan 2022
  6. elifesciences.org elifesciences.org
    Evolutionary transcriptomics implicates new genes and pathways in human pregnancy and adverse pregnancy outcomes
    9
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    elifesciences.org/articles/69584
  7. elifesciences.org elifesciences.org
    Reproducibility in Cancer Biology: Challenges for assessing replicability in preclinical cancer biology
    1
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    elifesciences.org/articles/67995
  8. elifesciences.org elifesciences.org
    eLife and Medicine: Rigorous review and editorial oversight of clinical preprints
    3
    1. wangtaotao 03 Jan 2022
      Such opportunities and challenges, best illustrated during the pandemic, lie ahead as medicine moves towards the goal of providing evidence-based care to patients no matter where they live. This trajectory also opens up real opportunities for speed, transparency and rich evaluation across peer review in medicine.

      呼吁医学界提供更为快速、透明、评估更为全面的同行评审创造需求

    2. wangtaotao 03 Jan 2022
      But even as the pandemic has demonstrated the potential for rapid, author-driven publication to democratize access and accelerate research, it has exposed the challenges of this model of scholarly communication

      作者驱动的快速出版 面向大众开放 促进研究

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    elifesciences.org/articles/67528
  9. Dec 2021
  10. elifesciences.org elifesciences.org
    Specialized neurons in the right habenula mediate response to aversive olfactory cues
    9
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    elifesciences.org/articles/72345
  11. elifesciences.org elifesciences.org
    IL-2/JES6-1 mAb complexes dramatically increase sensitivity to LPS through IFN-γ production by CD25+Foxp3- T cells
    29
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    elifesciences.org/articles/62432
  12. elifesciences.org elifesciences.org
    Light sets the brain’s daily clock by regional quickening and slowing of the molecular clockworks at dawn and dusk
    4
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    elifesciences.org/articles/70137
  13. homandev.pressbooks.network homandev.pressbooks.network
    Webinar Report: Being a scientist and a parent
    1
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    homandev.pressbooks.network/emptybook/
  14. Nov 2021
  15. elifesciences.org elifesciences.org
    Research Culture: Creating SPACE to evolve academic assessment
    6
    1. dasaptaerwin 10 Nov 2021
      it is designed to help institutional leaders reflect on the extent to which their organization can support sustained and values-driven assessment practices, and where they might focus efforts to further evolve them.

      SPACE to support sustained and values-driven assessment practices.

      research culture
    5. dasaptaerwin 10 Nov 2021
      Rather, it is due to a growing reliance on proxy measures of research quality in the management of recruitment, promotion, tenure and funding decisions: these proxy measures are widely used because they are convenient, not because they are meaningful. The pursuit of a higher ranking in league tables for universities has also contributed to the problem.

      proxy measures >> convenient but not meaningful

      research culture proxy
    6. dasaptaerwin 10 Nov 2021
      Ghent University in Belgium made headlines in 2019 when it announced a new policy for evaluating faculty that marked a shift away from the 'rat race' of metrics and rankings towards more holistic processes focused on valuing and nurturing talent

      notice >> rat race

      research culture
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    elifesciences.org/articles/70929
  16. Sep 2021
  17. elifesciences.org elifesciences.org
    Single-molecule observation of ATP-independent SSB displacement by RecO in Deinococcus radiodurans
    5
    1. aksmit22 22 Sep 2021
      However, the entire repair process for DSBs is notably more efficient in DR than in other organisms; DR is able to restore hundreds of copies of damaged double strands in an hour

      I guess that answers my last question, but raises another question: what allows the repair process to only be most efficient in DR than other organisms, could there be some other important aspect involved in the mechanism?

    2. aksmit22 22 Sep 2021
      The first binding between drRecO and ssDNA occurs in the drSSB-free space of ssDNA and generates a heterotrimer of the drSSB-ssDNA-drRecO complex as an intermediate state. The second binding between drRecO and ssDNA induces the dissociation of drSSB from ssDNA by facilitating a conformational change in ssDNA.

      So ultimately from these findings there are two steps involved in a conformational change in the ssDNA and can avoid using ATP?

    3. aksmit22 22 Sep 2021
      molecular mechanism of D. radiodurans (DR) RecO (drRecO) in SSB displacement from ssDNA at the single-molecule level. DR is the toughest bacterium known, with outstanding resistance to ionizing radiation and DNA damage-inducing reagents (Blasius et al., 2008; Cox and Battista, 2005; Slade and Radman, 2011), which are the main causes of DSBs, a form of fatal biological damage at the genomic level

      I wonder if a section of the genome that helps encode for this mechanism and trait for resistance to ionizing radiation can be inserted in other organisms or be used to prevent DNA damage from radiation

    4. wcmarx 21 Sep 2021
      In summary, our results demonstrate that drRecO binds to ssDNA sequentially using its two binding sites, which gives drRecO the ability to displace drSSB from ssDNA even though the binding affinity of drRecO for ssDNA is two orders of magnitude smaller than that of drSSB.

      It is amazing that cells are capable of performing actions like this and the researchers were able to show this through their results. I would usually assume that a molecule would need a stronger binding affinity to displace another bound protein, so it was interesting to learn how DrRecO can accomplish this with a much lower binding affinity.

    5. tkduncan 21 Sep 2021
      RecO is believed to play a key role in this step because RecO is able to interact with SSB, ssDNA, and dsDNA simultaneously

      So (if I'm interpreting this correctly) RecO is thought to be involved in helping to recruit the rest of the RecFOR machinery to the site of the break because it can interact with SSB, ssDNA, and dsDNA even though SSB binds strongly to ssDNA, right?

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    elifesciences.org/articles/50945
  18. Aug 2021
  19. elifesciences.org elifesciences.org
    Natural variation in the consequences of gene overexpression and its implications for evolutionary trajectories
    2
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    elifesciences.org/articles/70564
  20. elifesciences.org elifesciences.org
    The GNU subunit of PNG kinase, the developmental regulator of mRNA translation, binds BIC-C to localize to RNP granules
    14
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    elifesciences.org/articles/67294
  21. elifesciences.org elifesciences.org
    An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites
    6
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    elifesciences.org/articles/68268
  22. elifesciences.org elifesciences.org
    HOXA9 promotes MYC-mediated leukemogenesis by maintaining gene expression for multiple anti-apoptotic pathways
    44
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    elifesciences.org/articles/64148
  23. elifesciences.org elifesciences.org
    miR-1 coordinately regulates lysosomal v-ATPase and biogenesis to impact proteotoxicity and muscle function during aging
    7
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    elifesciences.org/articles/66768
  24. elifesciences.org elifesciences.org
    VEGF/VEGFR2 signaling regulates hippocampal axon branching during development
    6
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    elifesciences.org/articles/49818
  25. elifesciences.org elifesciences.org
    Soluble PD-L1 generated by endogenous retroelement exaptation is a receptor antagonist
    1
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    elifesciences.org/articles/50256
  26. elifesciences.org elifesciences.org
    A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in brain neurons
    2
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    elifesciences.org/articles/48750
  27. elifesciences.org elifesciences.org
    Taste bud formation depends on taste nerves
    1
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    elifesciences.org/articles/49226
  28. elifesciences.org elifesciences.org
    An open cortico-basal ganglia loop allows limbic control over motor output via the nigrothalamic pathway
    3
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    elifesciences.org/articles/49995
  29. elifesciences.org elifesciences.org
    CCN1 interlinks integrin and Hippo pathway to autoregulate tip cell activity
    1
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    elifesciences.org/articles/46012
  30. elifesciences.org elifesciences.org
    Mutationally-activated PI3’-kinase-α promotes de-differentiation of lung tumors initiated by the BRAFV600E oncoprotein kinase
    1
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    elifesciences.org/articles/43668
  31. elifesciences.org elifesciences.org
    Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 crosstalk
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    elifesciences.org/articles/47646
  32. elifesciences.org elifesciences.org
    RbFox1 up-regulation impairs BDNF-dependent hippocampal LTP by dysregulating TrkB isoform expression levels
    1
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    elifesciences.org/articles/49673
  33. elifesciences.org elifesciences.org
    Ataxin-7 and Non-stop coordinate SCAR protein levels, subcellular localization, and actin cytoskeleton organization
    1
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    elifesciences.org/articles/49677
  34. elifesciences.org elifesciences.org
    Epimutations are associated with CHROMOMETHYLASE 3-induced de novo DNA methylation
    1
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    elifesciences.org/articles/47891
  35. elifesciences.org elifesciences.org
    The antimicrobial peptide defensin cooperates with tumour necrosis factor to drive tumour cell death in Drosophila
    1
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    elifesciences.org/articles/45061
  36. elifesciences.org elifesciences.org
    Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    4
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    elifesciences.org/articles/18825
  37. elifesciences.org elifesciences.org
    27 T ultra-high static magnetic field changes orientation and morphology of mitotic spindles in human cells
    8
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    elifesciences.org/articles/22911
  38. elifesciences.org elifesciences.org
    A Cryptochrome 2 mutation yields advanced sleep phase in humans
    7
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    elifesciences.org/articles/16695
  39. elifesciences.org elifesciences.org
    The retromer complex safeguards against neural progenitor-derived tumorigenesis by regulating Notch receptor trafficking
    2
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    elifesciences.org/articles/38181
  40. elifesciences.org elifesciences.org
    GIPC proteins negatively modulate Plexind1 signaling during vascular development
    1
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    elifesciences.org/articles/30454