- Aug 2021
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onlinelibrary.wiley.com onlinelibrary.wiley.com
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RRID:ZFIN_ZDB-GENO-090918-6
DOI: 10.1002/cne.23645
Resource: (ZFIN Cat# ZDB-GENO-090918-6,RRID:ZFIN_ZDB-GENO-090918-6)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-GENO-090918-6
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- Jul 2021
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www.sciencedirect.com www.sciencedirect.com
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Zebrafish: Tg(sox10(7.2):mEGFP)sl3
DOI: 10.1016/j.cell.2019.08.001
Resource: (ZFIN Cat# ZDB-ALT-150113-6,RRID:ZFIN_ZDB-ALT-150113-6)
Curator: @ethanbadger
SciCrunch record: RRID:ZFIN_ZDB-ALT-150113-6
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www.sciencedirect.com www.sciencedirect.com
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Zebrafish: npas4ls5 mutant
DOI: 10.1016/j.celrep.2019.05.052
Resource: (ZFIN Cat# ZDB-ALT-010426-6,RRID:ZFIN_ZDB-ALT-010426-6)
Curator: @ethanbadger
SciCrunch record: RRID:ZFIN_ZDB-ALT-010426-6
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RRID: ZDB-ALT-171004-6
DOI: 10.7554/eLife.25727
Resource: (ZFIN Cat# ZDB-ALT-171004-6,RRID:ZFIN_ZDB-ALT-171004-6)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-171004-6
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Tg(kdrl:eGFP)
DOI: 10.1364/BOE.6.002056
Resource: (ZFIN Cat# ZDB-ALT-061120-6,RRID:ZFIN_ZDB-ALT-061120-6)
Curator: @mpairish
SciCrunch record: RRID:ZFIN_ZDB-ALT-061120-6
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Tg(UAS:kaede)rk8
DOI: 10.7554/eLife.07288
Resource: (ZFIN Cat# ZDB-ALT-040913-6,RRID:ZFIN_ZDB-ALT-040913-6)
Curator: @mpairish
SciCrunch record: RRID:ZFIN_ZDB-ALT-040913-6
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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cftrpd1048
DOI: 10.1002/dvdy.24355
Resource: (ZFIN Cat# ZDB-GENO-121210-6,RRID:ZFIN_ZDB-GENO-121210-6)
Curator: @mpairish
SciCrunch record: RRID:ZFIN_ZDB-GENO-121210-6
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZDB-ALT-040217-6
DOI: 10.7554/eLife.37001
Resource: (ZFIN Cat# ZDB-ALT-040217-6,RRID:ZFIN_ZDB-ALT-040217-6)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-040217-6
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RRID:ZDB-ALT-110310-6
DOI: 10.7554/eLife.37001
Resource: (ZFIN Cat# ZDB-ALT-110310-6,RRID:ZFIN_ZDB-ALT-110310-6)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-110310-6
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onlinelibrary.wiley.com onlinelibrary.wiley.com
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ZFIN_ZDB-GENO-090918-6
DOI: 10.1002/cne.23645
Resource: (ZFIN Cat# ZDB-GENO-090918-6,RRID:ZFIN_ZDB-GENO-090918-6)
Curator: @gabimpine
SciCrunch record: RRID:ZFIN_ZDB-GENO-090918-6
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ZFIN_ZDB-GENO-090918-6
DOI: 10.1002/cne.23645
Resource: (ZFIN Cat# ZDB-GENO-090918-6,RRID:ZFIN_ZDB-GENO-090918-6)
Curator: @gabimpine
SciCrunch record: RRID:ZFIN_ZDB-GENO-090918-6
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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ZFIN ID: ZDB-ALT-120409-6
DOI: 10.7554/eLife.66596
Resource: (ZFIN Cat# ZDB-ALT-120409-6,RRID:ZFIN_ZDB-ALT-120409-6)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-120409-6
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- Jun 2021
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www.sciencedirect.com www.sciencedirect.com
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ZFIN: ZDB-ALT-131127-6
DOI: 10.1016/j.cub.2021.05.042
Resource: (ZFIN Cat# ZDB-ALT-131127-6,RRID:ZFIN_ZDB-ALT-131127-6)
Curator: @Naa003
SciCrunch record: RRID:ZFIN_ZDB-ALT-131127-6
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ZFIN: ZDB-ALT-110217-6
DOI: 10.1016/j.cub.2021.05.042
Resource: (ZFIN Cat# ZDB-ALT-110217-6,RRID:ZFIN_ZDB-ALT-110217-6)
Curator: @Naa003
SciCrunch record: RRID:ZFIN_ZDB-ALT-110217-6
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elifesciences.org elifesciences.org
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ZDB-ALT-051223–6
DOI: 10.7554/eLife.62155
Resource: (ZFIN Cat# ZDB-ALT-051223-6,RRID:ZFIN_ZDB-ALT-051223-6)
Curator: @Naa003
SciCrunch record: RRID:ZFIN_ZDB-ALT-051223-6
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academic.oup.com academic.oup.com
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Sarilumab and Tocilizumab
Another open-label trial NCT02735707 showed that both IL-6 receptor antagonists improved recovery and survival compared to standard care https://doi.org/10.1101/2021.01.07.21249390 (preprint)
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- May 2021
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www.sciencedirect.com www.sciencedirect.com
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ZFIN: ZDB-ALT-051111-6
DOI: 10.1016/j.devcel.2020.07.015
Resource: (ZFIN Cat# ZDB-ALT-051111-6,RRID:ZFIN_ZDB-ALT-051111-6)
Curator: @Naa003
SciCrunch record: RRID:ZFIN_ZDB-ALT-051111-6
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ZFIN: ZDB-ALT-051223-6
DOI: 10.1016/j.devcel.2020.07.015
Resource: (ZFIN Cat# ZDB-ALT-051223-6,RRID:ZFIN_ZDB-ALT-051223-6)
Curator: @Naa003
SciCrunch record: RRID:ZFIN_ZDB-ALT-051223-6
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ZFIN: ZDB-GENO-200316-6
DOI: 10.1016/j.devcel.2020.07.015
Resource: (ZFIN Cat# ZDB-GENO-200316-6,RRID:ZFIN_ZDB-GENO-200316-6)
Curator: @Naa003
SciCrunch record: RRID:ZFIN_ZDB-GENO-200316-6
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- Mar 2021
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www.nord.gouv.fr www.nord.gouv.fr
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CONSEQUENCES JURIDIQUES DU DEFAUT D’ACCUSE DE RECEPTION. Article L112-6 CRPASi l’administration n’accuse pas réception de la demande, ou le fait demanière incorrecte (mentions incomplètes ou erronées), les délais derecours ne sont pas opposables à l’auteur de la demande.L’usager pourra donc contester votre décision implicite à tout momentdevant la juridiction administrative.Il est donc important pour la sécurité juridique de vos décisions, derespecter les règles tant sur la forme que sur le fond.
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Results for individual PALB2 variants were normalized relative to WT-PALB2 and the p.Tyr551ter (p.Y551X) truncating variant on a 1:5 scale with the fold change in GFP-positive cells for WT set at 5.0 and fold change GFP-positive cells for p.Y551X set at 1.0. The p.L24S (c.71T>C), p.L35P (c.104T>C), p.I944N (c.2831T>A), and p.L1070P (c.3209T>C) variants and all protein-truncating frame-shift and deletion variants tested were deficient in HDR activity, with normalized fold change <2.0 (approximately 40% activity) (Fig. 1a).
AssayResult: 4.7
AssayResultAssertion: Normal
StandardErrorMean: 0.32
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A total of 84 PALB2 patient-derived missense variants reported in ClinVar, COSMIC, and the PALB2 LOVD database were selected
HGVS: NM_024675.3:c.109C>T p.(Arg37Cys)
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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SUPPLEMENTARY DATA
AssayResult: 93.06
AssayResultAssertion: Not reported
PValue: > 0.9999
Comment: Exact values reported in Table S3.
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To this end, 44 missense variants found in breast cancer patients were identified in the ClinVar database (https://www.ncbi.nlm.nih.gov/clinvar) and/or selected by literature curation based on their frequency of description or amino acid substitution position in the protein (Supplemental Table S1).
HGVS: NM_024675.3:c.226A>G p.(Ile76Val)
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Source Data
AssayResult: 17.43
AssayResultAssertion: Abnormal
ReplicateCount: 2
StandardErrorMean: 5.19
Comment: Exact values reported in “Source Data” file.
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Source Data
AssayResult: 7.82
AssayResultAssertion: Abnormal
ReplicateCount: 2
StandardDeviation: 2.31
StandardErrorMean: 1.64
Comment: Exact values reported in “Source Data” file.
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We, therefore, analyzed the effect of 48 PALB2 VUS (Fig. 2a, blue) and one synthetic missense variant (p.A1025R) (Fig. 2a, purple)29 on PALB2 function in HR.
HGVS: NM_024675.3:c.1227_1231delTGTTA p.(Y409X)
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www.cell.com www.cell.com
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Most Suspected Brugada Syndrome Variants Had (Partial) Loss of Function
AssayResult: 0
AssayResultAssertion: Abnormal
ReplicateCount: 39
StandardErrorMean: 0
Comment: This variant had loss of function of peak current (<10% of wildtype), therefore it was considered abnormal (in vitro features consistent with Brugada Syndrome Type 1). (Personal communication: A. Glazer)
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we selected 73 previously unstudied variants: 63 suspected Brugada syndrome variants and 10 suspected benign variants
HGVS: NM_198056.2:c.1100G>T p.(Arg367Leu)
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZFIN_ZDB-ALT-050512-6
DOI: 10.7554/eLife.64267
Resource: (ZFIN Cat# ZDB-ALT-050512-6,RRID:ZFIN_ZDB-ALT-050512-6)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-050512-6
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elifesciences.org elifesciences.org
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RRID:ZFINID:ZDB-ALT-120523-6
DOI: 10.7554/eLife.63595
Resource: (ZFIN Cat# ZDB-ALT-120523-6,RRID:ZFIN_ZDB-ALT-120523-6)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-120523-6
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RRID:ZFINID:ZDB-ALT-160204-6
DOI: 10.7554/eLife.63595
Resource: (ZFIN Cat# ZDB-ALT-160204-6,RRID:ZFIN_ZDB-ALT-160204-6)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-160204-6
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- Feb 2021
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZFIN_ZDB-ALT-131127-6
DOI: 10.1126/sciadv.aaz3173
Resource: (ZFIN Cat# ZDB-ALT-131127-6,RRID:ZFIN_ZDB-ALT-131127-6)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-131127-6
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bpspubs.onlinelibrary.wiley.com bpspubs.onlinelibrary.wiley.com
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RRID:ZFIN_ZDB-ALT-160322-6
DOI: 10.1111/bph.15156
Resource: (ZFIN Cat# ZDB-ALT-160322-6,RRID:ZFIN_ZDB-ALT-160322-6)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-160322-6
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elifesciences.org elifesciences.org
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RRID:ZDB-ALT-040217-6
DOI: 10.7554/eLife.37001
Resource: (ZFIN Cat# ZDB-ALT-040217-6,RRID:ZFIN_ZDB-ALT-040217-6)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-040217-6
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RRID:ZDB-ALT-110310-6
DOI: 10.7554/eLife.37001
Resource: (ZFIN Cat# ZDB-ALT-110310-6,RRID:ZFIN_ZDB-ALT-110310-6)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-110310-6
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elifesciences.org elifesciences.org
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RRID:ZFIN_ZDB-ALT-051223-6
DOI: 10.7554/eLife.42881
Resource: (ZFIN Cat# ZDB-ALT-051223-6,RRID:ZFIN_ZDB-ALT-051223-6)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-051223-6
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RRID:ZFIN_ZDB-ALT-160323-6
DOI: 10.7554/eLife.42881
Resource: (ZFIN Cat# ZDB-ALT-160323-6,RRID:ZFIN_ZDB-ALT-160323-6)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-160323-6
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jmg.bmj.com jmg.bmj.com
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Supplemental material
AssayResult: 89, 90
AssayResultAssertion: Normal
Comment: See Table S3 for details
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Supplemental material
AssayResult: 5.8, 6.1
AssayResultAssertion: Abnormal
Comment: See Table S3 for details
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We analysed a total of 82 blood samples derived from 77 individuals (online supplemental table 3). These 77 individuals corresponded either to new index cases suspected to harbour a pathogenic TP53 variant or to relatives of index cases harbouring TP53 variants.
HGVS: NM_000546.5:c.1043T>C p.(Leu348Ser)
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laulima.hawaii.edu laulima.hawaii.edu
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‘ofa‘ang
love or lover
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10.11.66.200 10.11.66.200
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First and Last Name
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If the cardholder refuses to be transferred to the IVR
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- Jan 2021
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Local file Local file
- Oct 2020
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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ZFIN : ZDB-ALT-160119–6
DOI: 10.7554/eLife.59469
Resource: (ZFIN Cat# ZDB-ALT-160119-6,RRID:ZFIN_ZDB-ALT-160119-6)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-160119-6
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ecourse.auca.kg ecourse.auca.kgFYS.pdf2
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He who sees me everywhereand sees everything in mewill not be lost to me,and I will not be lost to him
Is it explaining the relationship between lord Krishna and Arjuna?
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A man of discipline should alwaysdiscipline himself, remain in seclusion,isolated, his thought and self well controlled,without possessions or hope.
A man of discipline is the one who should control his senses, and he should be discipline by himself.
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www.americanyawp.com www.americanyawp.com
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I am very intrigued by the arguments that are mentioned by Angeline Grimke'. These are perspectives that I have not considered other than the morality aspect situation. I do like the way that she compares America, where we enslave men, women, and children but even in Africa they would be free. Not only free but have many more inalienable rights in such a poor, egregious country, in my opinion of the times anyway. But here in the land of the free they can't control one single facet of their life. And how exactly does a person being born in America, that is as American as George Washington, lose his rights because one man says so and believes that he is subservient in nature. I would not have done well back then. The ignorance that they posses is devastatingly incredible. That total lack of consideration for other people and human life is utterly despicable. To think that the men could not even get it figured out. It was such a long lasting debate that women had to get involved and try to speak some sort of since to these so called men. I know it is a different time but I am unable to fathom treating someone so horribly. I am glad it is a different time!
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www.youtube.com www.youtube.com
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à 21.20 les écrans à tous les âges résultats de l'étude en 2027 https://youtu.be/ovbeMGfSO2M?t=1284
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Annotators
URL
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- Sep 2020
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cosmicchrist.net cosmicchrist.net
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The answer is that they were two different “Yahweh’s.”
OK. I'm trying to work with this. I feel like I've been on the edge of revelation concerning this for quite a while.
- Wouldn't the 1st encounter w/ a negative entity be in Genesis (Specifically because of all the killing and wars and Gen 6 account)
- Was the serpent a negative entity????
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ecourse.auca.kg ecourse.auca.kgFYS.pdf1
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She persuades Anu, her father, to give her the fiery Bull of Heaven (the constellation Taurus) so that she can punish Gilgamesh with death.
Why she wanted to kill Gilgamesh, if she loves him?
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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ZDB-ALT-170424-6
DOI: 10.1016/j.devcel.2020.03.017
Resource: (ZFIN Cat# ZDB-ALT-170424-6,RRID:ZFIN_ZDB-ALT-170424-6)
Curator: @ethanbadger
SciCrunch record: RRID:ZFIN_ZDB-ALT-170424-6
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- Aug 2020
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www.sciencedirect.com www.sciencedirect.com
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ZDB-ALT-120320-6
DOI: 10.1016/j.cub.2020.05.016
Resource: (ZFIN Cat# ZDB-ALT-120320-6,RRID:ZFIN_ZDB-ALT-120320-6)
Curator: @ethanbadger
SciCrunch record: RRID:ZFIN_ZDB-ALT-120320-6
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www.indepaz.org.co www.indepaz.org.co
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Los Acuerdos PDET, así sean aún incipientes y en construcción, concebidos como procesos, deberían dar lugar a proyectos y acciones concretos, en algunos casos de tipo experimental o piloto. Esto, por ejemplo, en cuanto a proyectos productivos de la estrategia de “territorios agroalimentarios
Se responde en parte la pregunta de cómo se articulan Territorios agroalimentarios campesinos y PDET
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- Jun 2020
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www.defenseurdesdroits.fr www.defenseurdesdroits.fr
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L’article 6 fixe un second principe général qui reconnait le droit inhérent à la vie de tout enfant et l’obligation de l’État d’assurer sa survie et son développement
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www.behaviouralbydesign.co.nz www.behaviouralbydesign.co.nz
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5 Innovations in Behavioural Design for Physical Distancing. (2020 May 06) https://www.behaviouralbydesign.co.nz/post/5-innovations-in-behavioural-design-for-physical-distancing
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- Jan 2020
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZFIN_ZDB-ALT-020918-6
DOI: 10.7554/eLife.46275
Resource: (ZFIN Cat# ZDB-ALT-020918-6,RRID:ZFIN_ZDB-ALT-020918-6)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-020918-6
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZFIN_ZDB-ALT-150424-6
DOI: 10.7554/eLife.42455
Resource: (ZFIN Cat# ZDB-ALT-150424-6,RRID:ZFIN_ZDB-ALT-150424-6)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-150424-6
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www.sciencedirect.com www.sciencedirect.com
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allele id lookup result: http://reg.clinicalgenome.org/redmine/projects/registry/genboree_registry/by_caid?caid=CA399189115
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down-slanting eyelid
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0200006
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short proximal extremities
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0009815
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6
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000175
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6
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000369
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6
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000316
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6
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000348
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6
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0002003
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macrocephaly
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000256
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muscular hypotonia
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001252
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intellectual disability
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001249
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motor development
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001270
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speech and language development
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000750
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developmental delay
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001263
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- Dec 2019
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www.sciencedirect.com www.sciencedirect.com
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General hypotonia
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001290
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- Sep 2019
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Linearity
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- Jul 2019
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www.milestonedocuments.com www.milestonedocuments.com
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5. To accept the collaboration in Serbia of representatives of the Austro-Hungarian Government in the suppression of the subversive movement directed against the territorial integrity of the monarchy;
This demand was not met leading to be one of the causes of WWI.
Tags
Annotators
URL
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www.milestonedocuments.com www.milestonedocuments.com
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The removal, so far as possible, of all economic barriers and the establishment of an equality of trade conditions among all the nations consenting to the peace and associating themselves for its maintenance.
Free trade
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Adequate guarantees given and taken that national armaments will be reduced to the lowest point consistent with domestic safety.
reduction of national weapons
Tags
Annotators
URL
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Animal handling and care
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Cell culture
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Tests for Flavonoids
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Inoculum preparation
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Running of gel and visualization of DNA
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Enzyme production (EP) medium
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Mycelial characteristicsof pathogen
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Identificationof bioagent
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Isolation and purification of Trichoderma sp.
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Isolation and purification of pathogen, Fusarium udum
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Isolation, purification and morphological characterization of pathogen and bioagent
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Prevalence of type III secretion system genes amongV. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood
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Production of chitinase
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Resistance to ampicillin
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Citrate utilisation tes
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Nitrate reduction test
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Production of β-galactosidase (ortho-Nitrophenyl β-D-galactopyranoside (ONPG)) test
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Urease production
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Gelatinase production
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Growth at different temperatures
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Salt tolerance tes
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Voges Proskauer (acetoin production) test
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Indole production
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Carbon source utilisation tes
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Carbohydrate fermentation test
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Amino acids utilisation test (Decarboxylase/dihydrol
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Species level identification
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Urease productio
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Gelatinase productio
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Growth at different temperature
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Salt tolerance tes
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Voges Proskauer (acetoin production) tes
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Indole production
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Carbon source utilisation t
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Carbohydrate fermentation tes
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Amino acids utilisation test (Decarboxylase/dihydrola
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Species level identification
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Relative antibiotic resistance amongVibrioisolated from Cochin estuary, shrimp farm and seafood
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Motif analysis
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Phylogenetic and motif analysi
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In silico characterization of cloned Dof gene
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Gel elution of PCR Produ
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Scoring of amplification data points and construction of a den
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Analysis of PCR amplicons using agarose gel electrophoresis
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Cycling condition
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PCR reaction set up
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Basic requirements for PC
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PCR amplification of Dof domain and Dof genes from different
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Post-challenge survival
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Solutions used for cytokine assay
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Assessment of Cytokine levels- IFN--12/IL-10 in lymphocytes of cured/endemic patients
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Nitrite production in macrophages of hamsters
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Assessment of Lymphocyte proliferative responses (LTT) in cured/exposed patients and hamsters
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Immunological assays
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Amplification, Cloning and Sequencing
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Cloning, Overexpression and purificationof TP
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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2D QSAR
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Glutathione peroxidase (GPx) assay
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Superoxide dismutase (SOD) assay
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Catalase assay
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Assay for reduced glutathione(GSH)
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Assay for Lipid peroxidase(LPO)
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Estimation of Total protein
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Oxidative stress parameters
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Hydrogen peroxide quenching activity
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Nitric oxide radical quenching activity
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Superoxide radical scavenging activity
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Reducing power assay
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DPPH free radical quenching activity
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In vitro free radical scavenging assays
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Inhibition of haemocytes spreading behavior
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. Inhibition of haemocytes aggregation behavior
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Haemolymph protein profiling
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Total haemocyte count (THC)
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Haemolymph collection
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Immunomodulatory
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Alkaline Phosphatase
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Acid Phosphatase
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Estimation of Phosphatases activities (Acid and Alkaline Phosphatase) in muscle and gill tissue of fish, Labeo rohita
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- Jun 2019
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krishikosh.egranth.ac.in krishikosh.egranth.ac.in
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1000 Kernel weight(mg)
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Carbon sources
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Dominance of regulatory transitional B cellsand lower memory B cells in HBsAgpositive compared to negative newborns
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Separation of Peripheral Blood Mononuclear Cells(PBMCs)from whole blood
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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GST elution buffer
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Nuclear and cytoplasmic fractionation
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Secondary antibodies
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Primary antibodies
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Antibodies used in our study
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GFP-β-catenin-C-N (151-423 a.a) (ARM-N)
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Annexin-V FITC/ PI staining
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Antibodies
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Irradiation of bacteria
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.inThesis4
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Procedure
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Extraction of Tannin
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Reagent
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Tannin
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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ELISA blocking buffer
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Lymphoproliferation
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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DNA fragment isolation from agarose gels
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Resuspension solution
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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T-Cell Receptor Mutation Assay
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Modified Enzyme Linked Immunosorbent Assay
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Reducing powe
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Estimation of Steviol
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Extraction from plant material
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Quantification of steviol
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Endomorph-ectom9££E:
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Calf
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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V8 protease-mediated semisynthesis of a globin was carried out at 4°C in 0.05 M ammonium acetate buffer (pH 6) containing 30% 1-propanol. For this, the lyophilized samples of natural or synthetic analogs of a 1-30 and respective a31-141 were individually prepared in water. Suitable volumes of the complementary fragments were mixed to obtain a 1:1 molar ratio and lyophilized. The lyophilized material was dissolved in appropriate amount of ammonium acetate buffer (pH 6). To this solution, a suitable volume of 1-propanol was added to a final concentration of 30% 1-propanol and 20 mg/ml substrate. The mixture was cooled on ice subsequent to which suitable volume of V8 protease solution prepared in water ( 1% w/w of substrate) was added. The ligation reaction mixture was incubated at 4°C for 24 hours. The extent of synthesis was monitored on RPHPLC by loading an aliquot of the reaction mixture on an analytical reverse phase column. The reaction was stopped by addition of chilled 5% TF A solution (0.2 fold v/v) and lyophilized
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Construction of mutant a globins
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- May 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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The reaction mixture contained 0.2 mL of enzyme sample, 0.3 mL of buffer and 0.5 mL of p-nitrophenyl-β-D-glucopyranoside (1.0 mM) prepared in 100 mM buffer as the substrate. The reaction was terminated after 30 min of incubation at 70 °C by adding 2 mL of sodium carbonate-bicarbonate buffer (0.1 M, pH 10.0). The liberation of p-nitrophenol was measured at 400 nm and its yield was determined using a standard curve of p-nitrophenol (1-10 μg mL-1) prepared in sodium carbonate-bicarbonate buffer
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β-Glucosidase
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Overnight grown cultures of E. coli DH5α, E. coli BL21 (DE3), E. coli XL1blue cells with and without constructs were preserved in 80 % v/v glycerol
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MAINTENANCE OF THE RECOMBINANT STRAIN
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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incubated on ice for 5 min, buffer N3 (350 J.!l) was added to the mixture and the tube was iriverted 4-6 times until mix appeared cloudy. Cell debris was removed by centrifugation at 12000 x g for 10 min and the supernatant was applied to QIAprep spin columns. Columns were centrifuged at 12000 x g for 1 miri and the flow through was discarded and columns were washed using 750 J.!l of 70% ethanol and centrifuged, at 12000 x g for 1 min. Additional centrifugation was performed to remove the residual ethanol. The columns were placed in a 1.5 ml microfuge tube and DNA was eluted with autoclaved water or 1 mM Tris-HCI (PH 8.0).
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Plasmid DNA was extracted using commercially available kit (Qiagen, Germany) as per manufacturer's instructions. For a miniprep, bacterial cell pellet from 5ml freshly grown culture were resuspended in 250 III buffer PI containing RNaseA in a microfuge tube, followed by lysis in 250 III of buffer P2. After the tube was
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Plasmid DNA Isolation
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medium. The culture volume in 75 cm2 culture flasks, was increased to 25 ml from 12 ml. The flasks were kept at 37°C and the medium was prewarmed before use. The flasks were gassed with a mixture of 5% C02, 3% 02 and 92% N2 for a i minimum of 20 seconds at a pressure of around 5 Ib/in2. The culture medium was changed daily without the addition of RBCs. Blood smears were prepared once or twice a week to check the ~tate of the cultures and the presence of gametocytes. Typically, mature gametocytes were observed after 14-17 days
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Fresh stock of parasites was thawed for culture as described above. Thin blood smears were made on the fourth day after setting up the culture. When high , parasitemia with "stressed" parasites was observed, culture volume was increased by the addition of medium. At this stage, fresh RBCs were not added to the culture
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Gametocyte cultures
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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700 mM NaCl, 12.5 mM CaCh, pH 7.4). 5 J.!L of Annexin-V conjugated to Alexa fluor 488 and 1 J.!L of working solution of PI (100 Jlg/mL) were added to the 100 J.!L cell suspension. Cells were incubated for 15 min at room temperature. Following this, 400 J.!L of IX Annexin binding buffer was added to dilute the sample. The samples were placed on ice. The fluorescence was measured by flow cytometry in FL 1 and FL2 channels for Annexin-V-Alexa fluor 488 and PI fluorescence respectively.
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The Vybrant apoptosis assay kit was used to perform Annexin-V/PI staining as described previously (3). The assay is based on the principle that apoptotic cells show loss of membrane asymmetry by exposing phosphatidylserine on the outer surface of the plasma membrane for which Annexin-V, a phosphlipid binding protein, shows high affinity. Hence, Annexin-V conjugated to Alexa fluor 488 binds to phosphatidylserine exposed on apoptotic cells, while propidium iodide binds to nucleic acids of all non-viable cells including necrotic and apoptotic cells. Thus, flow-cytometric analysis of Annexin-V /PI stained cells reveals distinct cellular populations, with the viable cells displaying little or no fluorescence; the early apoptotic cells show green fluorescence of Annexin-V conjugated to Alexa fluor 488; the late apoptotic cells display both green and red fluorescence, while necrotic cells show red fluorescence. The cells after appropriate treatment were harvested by centrifugation at 250 x g for 5 min and were given two washes with ice-cold 1X PBS following which they were resuspended in 100 J!L of ice-cold 1X Annexin binding buffer (50 mM HEPES,
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