536 Matching Annotations
  1. Last 7 days
  2. Jun 2021
    1. ZFIN: ZDB-ALT-131127-6

      DOI: 10.1016/j.cub.2021.05.042

      Resource: (ZFIN Cat# ZDB-ALT-131127-6,RRID:ZFIN_ZDB-ALT-131127-6)

      Curator: @Naa003

      SciCrunch record: RRID:ZFIN_ZDB-ALT-131127-6


      What is this?

    2. ZFIN: ZDB-ALT-110217-6

      DOI: 10.1016/j.cub.2021.05.042

      Resource: (ZFIN Cat# ZDB-ALT-110217-6,RRID:ZFIN_ZDB-ALT-110217-6)

      Curator: @Naa003

      SciCrunch record: RRID:ZFIN_ZDB-ALT-110217-6


      What is this?

  3. May 2021
    1. ZFIN: ZDB-ALT-051111-6

      DOI: 10.1016/j.devcel.2020.07.015

      Resource: (ZFIN Cat# ZDB-ALT-051111-6,RRID:ZFIN_ZDB-ALT-051111-6)

      Curator: @Naa003

      SciCrunch record: RRID:ZFIN_ZDB-ALT-051111-6


      What is this?

    2. ZFIN: ZDB-ALT-051223-6

      DOI: 10.1016/j.devcel.2020.07.015

      Resource: (ZFIN Cat# ZDB-ALT-051223-6,RRID:ZFIN_ZDB-ALT-051223-6)

      Curator: @Naa003

      SciCrunch record: RRID:ZFIN_ZDB-ALT-051223-6


      What is this?

    3. ZFIN: ZDB-GENO-200316-6

      DOI: 10.1016/j.devcel.2020.07.015

      Resource: (ZFIN Cat# ZDB-GENO-200316-6,RRID:ZFIN_ZDB-GENO-200316-6)

      Curator: @Naa003

      SciCrunch record: RRID:ZFIN_ZDB-GENO-200316-6


      What is this?

  4. Mar 2021
    1. CONSEQUENCES JURIDIQUES DU DEFAUT D’ACCUSE DE RECEPTION. Article L112-6 CRPASi l’administration n’accuse pas réception de la demande, ou le fait demanière incorrecte (mentions incomplètes ou erronées), les délais derecours ne sont pas opposables à l’auteur de la demande.L’usager pourra donc contester votre décision implicite à tout momentdevant la juridiction administrative.Il est donc important pour la sécurité juridique de vos décisions, derespecter les règles tant sur la forme que sur le fond.
    1. Results for individual PALB2 variants were normalized relative to WT-PALB2 and the p.Tyr551ter (p.Y551X) truncating variant on a 1:5 scale with the fold change in GFP-positive cells for WT set at 5.0 and fold change GFP-positive cells for p.Y551X set at 1.0. The p.L24S (c.71T>C), p.L35P (c.104T>C), p.I944N (c.2831T>A), and p.L1070P (c.3209T>C) variants and all protein-truncating frame-shift and deletion variants tested were deficient in HDR activity, with normalized fold change <2.0 (approximately 40% activity) (Fig. 1a).

      AssayResult: 4.7

      AssayResultAssertion: Normal

      StandardErrorMean: 0.32

    2. A total of 84 PALB2 patient-derived missense variants reported in ClinVar, COSMIC, and the PALB2 LOVD database were selected

      HGVS: NM_024675.3:c.109C>T p.(Arg37Cys)

    1. SUPPLEMENTARY DATA

      AssayResult: 93.06

      AssayResultAssertion: Not reported

      PValue: > 0.9999

      Comment: Exact values reported in Table S3.

    2. To this end, 44 missense variants found in breast cancer patients were identified in the ClinVar database (https://www.ncbi.nlm.nih.gov/clinvar) and/or selected by literature curation based on their frequency of description or amino acid substitution position in the protein (Supplemental Table S1).

      HGVS: NM_024675.3:c.226A>G p.(Ile76Val)

    1. Source Data

      AssayResult: 17.43

      AssayResultAssertion: Abnormal

      ReplicateCount: 2

      StandardErrorMean: 5.19

      Comment: Exact values reported in “Source Data” file.

    2. Source Data

      AssayResult: 7.82

      AssayResultAssertion: Abnormal

      ReplicateCount: 2

      StandardDeviation: 2.31

      StandardErrorMean: 1.64

      Comment: Exact values reported in “Source Data” file.

    3. We, therefore, analyzed the effect of 48 PALB2 VUS (Fig. 2a, blue) and one synthetic missense variant (p.A1025R) (Fig. 2a, purple)29 on PALB2 function in HR.

      HGVS: NM_024675.3:c.1227_1231delTGTTA p.(Y409X)

    1. Most Suspected Brugada Syndrome Variants Had (Partial) Loss of Function

      AssayResult: 0

      AssayResultAssertion: Abnormal

      ReplicateCount: 39

      StandardErrorMean: 0

      Comment: This variant had loss of function of peak current (<10% of wildtype), therefore it was considered abnormal (in vitro features consistent with Brugada Syndrome Type 1). (Personal communication: A. Glazer)

    2. we selected 73 previously unstudied variants: 63 suspected Brugada syndrome variants and 10 suspected benign variants

      HGVS: NM_198056.2:c.1100G>T p.(Arg367Leu)

  5. Feb 2021
    1. RRID:ZFIN_ZDB-ALT-160322-6

      DOI: 10.1111/bph.15156

      Resource: (ZFIN Cat# ZDB-ALT-160322-6,RRID:ZFIN_ZDB-ALT-160322-6)

      Curator: @scibot

      SciCrunch record: RRID:ZFIN_ZDB-ALT-160322-6


      What is this?

    1. RRID:ZDB-ALT-040217-6

      DOI: 10.7554/eLife.37001

      Resource: (ZFIN Cat# ZDB-ALT-040217-6,RRID:ZFIN_ZDB-ALT-040217-6)

      Curator: @scibot

      SciCrunch record: RRID:ZFIN_ZDB-ALT-040217-6


      What is this?

    2. RRID:ZDB-ALT-110310-6

      DOI: 10.7554/eLife.37001

      Resource: (ZFIN Cat# ZDB-ALT-110310-6,RRID:ZFIN_ZDB-ALT-110310-6)

      Curator: @scibot

      SciCrunch record: RRID:ZFIN_ZDB-ALT-110310-6


      What is this?

    1. RRID:ZFIN_ZDB-ALT-051223-6

      DOI: 10.7554/eLife.42881

      Resource: (ZFIN Cat# ZDB-ALT-051223-6,RRID:ZFIN_ZDB-ALT-051223-6)

      Curator: @scibot

      SciCrunch record: RRID:ZFIN_ZDB-ALT-051223-6


      What is this?

    2. RRID:ZFIN_ZDB-ALT-160323-6

      DOI: 10.7554/eLife.42881

      Resource: (ZFIN Cat# ZDB-ALT-160323-6,RRID:ZFIN_ZDB-ALT-160323-6)

      Curator: @scibot

      SciCrunch record: RRID:ZFIN_ZDB-ALT-160323-6


      What is this?

    1. Supplemental material

      AssayResult: 89, 90

      AssayResultAssertion: Normal

      Comment: See Table S3 for details

    2. Supplemental material

      AssayResult: 5.8, 6.1

      AssayResultAssertion: Abnormal

      Comment: See Table S3 for details

    3. We analysed a total of 82 blood samples derived from 77 individuals (online supplemental table 3). These 77 individuals corresponded either to new index cases suspected to harbour a pathogenic TP53 variant or to relatives of index cases harbouring TP53 variants.

      HGVS: NM_000546.5:c.1043T>C p.(Leu348Ser)

  6. Jan 2021
    1. PTT

      AQ:6 Please provide an expansion for PTT.

      PTT: partial thromboplastin time

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  7. Oct 2020
    1. He who sees me everywhereand sees everything in mewill not be lost to me,and I will not be lost to him

      Is it explaining the relationship between lord Krishna and Arjuna?

    2. A man of discipline should alwaysdiscipline himself, remain in seclusion,isolated, his thought and self well controlled,without possessions or hope.

      A man of discipline is the one who should control his senses, and he should be discipline by himself.

    1. I am very intrigued by the arguments that are mentioned by Angeline Grimke'. These are perspectives that I have not considered other than the morality aspect situation. I do like the way that she compares America, where we enslave men, women, and children but even in Africa they would be free. Not only free but have many more inalienable rights in such a poor, egregious country, in my opinion of the times anyway. But here in the land of the free they can't control one single facet of their life. And how exactly does a person being born in America, that is as American as George Washington, lose his rights because one man says so and believes that he is subservient in nature. I would not have done well back then. The ignorance that they posses is devastatingly incredible. That total lack of consideration for other people and human life is utterly despicable. To think that the men could not even get it figured out. It was such a long lasting debate that women had to get involved and try to speak some sort of since to these so called men. I know it is a different time but I am unable to fathom treating someone so horribly. I am glad it is a different time!

  8. Sep 2020
    1. The answer is that they were two different “Yahweh’s.”

      OK. I'm trying to work with this. I feel like I've been on the edge of revelation concerning this for quite a while.

      • Wouldn't the 1st encounter w/ a negative entity be in Genesis (Specifically because of all the killing and wars and Gen 6 account)
      • Was the serpent a negative entity????
    1. She persuades Anu, her father, to give her the fiery Bull of Heaven (the constellation Taurus) so that she can punish Gilgamesh with death.

      Why she wanted to kill Gilgamesh, if she loves him?

  9. Aug 2020
    1. Los Acuerdos PDET, así sean aún incipientes y en construcción, concebidos como procesos, deberían dar lugar a proyectos y acciones concretos, en algunos casos de tipo experimental o piloto. Esto, por ejemplo, en cuanto a proyectos productivos de la estrategia de “territorios agroalimentarios

      Se responde en parte la pregunta de cómo se articulan Territorios agroalimentarios campesinos y PDET

  10. Jun 2020
    1. L’article 6 fixe un second principe général qui reconnait le droit inhérent à la vie de tout enfant et l’obligation de l’État d’assurer sa survie et son développement
  11. Jan 2020
    1. RRID:ZFIN_ZDB-ALT-150424-6

      DOI: 10.7554/eLife.42455

      Resource: (ZFIN Cat# ZDB-ALT-150424-6,RRID:ZFIN_ZDB-ALT-150424-6)

      Curator: @evieth

      SciCrunch record: RRID:ZFIN_ZDB-ALT-150424-6


      What is this?

    1. down-slanting eyelid
    2. short proximal extremities
    3. 6
    4. 6
    5. 6
    6. 6
    7. 6
    8. macrocephaly
    9. muscular hypotonia
    10. intellectual disability
    11. motor development
    12. speech and language development
    13. developmental delay
  12. Dec 2019
  13. Sep 2019
  14. Jul 2019
    1. 5. To accept the collaboration in Serbia of representatives of the Austro-Hungarian Government in the suppression of the subversive movement directed against the territorial integrity of the monarchy;

      This demand was not met leading to be one of the causes of WWI.

    1. The removal, so far as possible, of all economic barriers and the establishment of an equality of trade conditions among all the nations consenting to the peace and associating themselves for its maintenance.

      Free trade

    2. Adequate guarantees given and taken that national armaments will be reduced to the lowest point consistent with domestic safety.

      reduction of national weapons

    1. Prevalence of type III secretion system genes amongV. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood
    2. Production of chitinase
    1. Resistance to ampicillin
    2. Citrate utilisation tes
    3. Nitrate reduction test
    4. Production of β-galactosidase (ortho-Nitrophenyl β-D-galactopyranoside (ONPG)) test
    5. Urease production
    6. Gelatinase production
    7. Growth at different temperatures
    8. Salt tolerance tes
    9. Voges Proskauer (acetoin production) test
    10. Indole production
    11. Carbon source utilisation tes
    12. Carbohydrate fermentation test
    13. Amino acids utilisation test (Decarboxylase/dihydrol
    14. Species level identification
    15. Urease productio
    16. Gelatinase productio
    17. Growth at different temperature
    18. Salt tolerance tes
    19. Voges Proskauer (acetoin production) tes
    20. Indole production
    21. Carbon source utilisation t
    22. Carbohydrate fermentation tes
    23. Amino acids utilisation test (Decarboxylase/dihydrola
    24. Species level identification
    1. Relative antibiotic resistance amongVibrioisolated from Cochin estuary, shrimp farm and seafood
  15. Jun 2019
    1. Dominance of regulatory transitional B cellsand lower memory B cells in HBsAgpositive compared to negative newborns
    1. V8 protease-mediated semisynthesis of a globin was carried out at 4°C in 0.05 M ammonium acetate buffer (pH 6) containing 30% 1-propanol. For this, the lyophilized samples of natural or synthetic analogs of a 1-30 and respective a31-141 were individually prepared in water. Suitable volumes of the complementary fragments were mixed to obtain a 1:1 molar ratio and lyophilized. The lyophilized material was dissolved in appropriate amount of ammonium acetate buffer (pH 6). To this solution, a suitable volume of 1-propanol was added to a final concentration of 30% 1-propanol and 20 mg/ml substrate. The mixture was cooled on ice subsequent to which suitable volume of V8 protease solution prepared in water ( 1% w/w of substrate) was added. The ligation reaction mixture was incubated at 4°C for 24 hours. The extent of synthesis was monitored on RPHPLC by loading an aliquot of the reaction mixture on an analytical reverse phase column. The reaction was stopped by addition of chilled 5% TF A solution (0.2 fold v/v) and lyophilized
    2. Construction of mutant a globins
  16. May 2019
    1. The reaction mixture contained 0.2 mL of enzyme sample, 0.3 mL of buffer and 0.5 mL of p-nitrophenyl-β-D-glucopyranoside (1.0 mM) prepared in 100 mM buffer as the substrate. The reaction was terminated after 30 min of incubation at 70 °C by adding 2 mL of sodium carbonate-bicarbonate buffer (0.1 M, pH 10.0). The liberation of p-nitrophenol was measured at 400 nm and its yield was determined using a standard curve of p-nitrophenol (1-10 μg mL-1) prepared in sodium carbonate-bicarbonate buffer
    2. β-Glucosidase
    3. Overnight grown cultures of E. coli DH5α, E. coli BL21 (DE3), E. coli XL1blue cells with and without constructs were preserved in 80 % v/v glycerol
    4. MAINTENANCE OF THE RECOMBINANT STRAIN
    1. incubated on ice for 5 min, buffer N3 (350 J.!l) was added to the mixture and the tube was iriverted 4-6 times until mix appeared cloudy. Cell debris was removed by centrifugation at 12000 x g for 10 min and the supernatant was applied to QIAprep spin columns. Columns were centrifuged at 12000 x g for 1 miri and the flow through was discarded and columns were washed using 750 J.!l of 70% ethanol and centrifuged, at 12000 x g for 1 min. Additional centrifugation was performed to remove the residual ethanol. The columns were placed in a 1.5 ml microfuge tube and DNA was eluted with autoclaved water or 1 mM Tris-HCI (PH 8.0).
    2. Plasmid DNA was extracted using commercially available kit (Qiagen, Germany) as per manufacturer's instructions. For a miniprep, bacterial cell pellet from 5ml freshly grown culture were resuspended in 250 III buffer PI containing RNaseA in a microfuge tube, followed by lysis in 250 III of buffer P2. After the tube was
    3. Plasmid DNA Isolation
    4. medium. The culture volume in 75 cm2 culture flasks, was increased to 25 ml from 12 ml. The flasks were kept at 37°C and the medium was prewarmed before use. The flasks were gassed with a mixture of 5% C02, 3% 02 and 92% N2 for a i minimum of 20 seconds at a pressure of around 5 Ib/in2. The culture medium was changed daily without the addition of RBCs. Blood smears were prepared once or twice a week to check the ~tate of the cultures and the presence of gametocytes. Typically, mature gametocytes were observed after 14-17 days
    5. Fresh stock of parasites was thawed for culture as described above. Thin blood smears were made on the fourth day after setting up the culture. When high , parasitemia with "stressed" parasites was observed, culture volume was increased by the addition of medium. At this stage, fresh RBCs were not added to the culture
    6. Gametocyte cultures
    1. 700 mM NaCl, 12.5 mM CaCh, pH 7.4). 5 J.!L of Annexin-V conjugated to Alexa fluor 488 and 1 J.!L of working solution of PI (100 Jlg/mL) were added to the 100 J.!L cell suspension. Cells were incubated for 15 min at room temperature. Following this, 400 J.!L of IX Annexin binding buffer was added to dilute the sample. The samples were placed on ice. The fluorescence was measured by flow cytometry in FL 1 and FL2 channels for Annexin-V-Alexa fluor 488 and PI fluorescence respectively.
    2. The Vybrant apoptosis assay kit was used to perform Annexin-V/PI staining as described previously (3). The assay is based on the principle that apoptotic cells show loss of membrane asymmetry by exposing phosphatidylserine on the outer surface of the plasma membrane for which Annexin-V, a phosphlipid binding protein, shows high affinity. Hence, Annexin-V conjugated to Alexa fluor 488 binds to phosphatidylserine exposed on apoptotic cells, while propidium iodide binds to nucleic acids of all non-viable cells including necrotic and apoptotic cells. Thus, flow-cytometric analysis of Annexin-V /PI stained cells reveals distinct cellular populations, with the viable cells displaying little or no fluorescence; the early apoptotic cells show green fluorescence of Annexin-V conjugated to Alexa fluor 488; the late apoptotic cells display both green and red fluorescence, while necrotic cells show red fluorescence. The cells after appropriate treatment were harvested by centrifugation at 250 x g for 5 min and were given two washes with ice-cold 1X PBS following which they were resuspended in 100 J!L of ice-cold 1X Annexin binding buffer (50 mM HEPES,
    3. Assay for detection of apoptosis by Annexin-V /PI staining
    1. function of resolution. The displacement for an isotropic B-value is related to the displacement u by the equation B = 8;(u2). The isotropic B-value assumes equal movements in all the directions. However, the vibration of an atom need not be the same in all the directions, and in such a case motion is described by anisotropic displacement parameter. In this formulation the motion is described by an ellipsoid that can be rotated in any direction. The entire anisotropic displacement can be described in terms of six elements: UIJ, U22 and U33 specify the magnitude of movement in three axis and U 12, U t3 and U23 specify the rotation off the principal axis. Anisotropic displacement parameters can be converted to the isotropic equivalent by the formula Biso = 8;(Ull+l'22+U33). The B-values are restrained during refinement. Atoms that are bonded to each other influence each other's motion. B-values are restrained in such a manner that the average difference in the B-values of bonded atoms is kept to a target value. The B-values should vary smoothly along the protein chain and within the side chain. The usual target restraint for adjacent bonded main chain atom is 1.0 and for side chain the target value is 1.5 since one end of the side chain is free, ensuring the higher gradient. Similarly B-values can be graded for the one to three members of a bond angle. For main chain angles the target value is 1.5 and for the side chain angle the value is set to 2.0. Like rigid body refinement, it is done at the early stages of the refinement process. Refinement of the atomic B-factors is a bit tricky and is carried out in later stages of refinement.
    2. The individual atoms were then refined by several cycles of conventional positional refinement, which uses the conjugate gradient minimization method. The proper weight term called Wa was calculated which was used for subsequent positional refinement (Brunger et al., 1987). In case of CNS this value was set to -1 and the program itself calculated these weights. The refinement was started using data in the range 50 A -4.0 A and higher resolution data were added in a stepwise fashion. After complete data had been added, the F0-Fc and 2F0-Fc electron density maps in addition to the composite omit map were calculated and displayed on an HP xw8400workstation (Hewlett-Packard Company, U.S.A.) using Coot (Emsley P, 2004 ). The electron density map was examined in