13,219 Matching Annotations
  1. Jun 2022
    1. RRID:AB_2535805

      DOI: 10.7554/eLife.78110

      Resource: (Thermo Fisher Scientific Cat# A-21236, RRID:AB_2535805)

      Curator: @scibot

      SciCrunch record: RRID:AB_2535805


      What is this?

    2. RRID:AB_10563566

      DOI: 10.7554/eLife.78110

      Resource: (Thermo Fisher Scientific Cat# A-11036, RRID:AB_10563566)

      Curator: @scibot

      SciCrunch record: RRID:AB_10563566


      What is this?

    3. RRID:AB_2534096

      DOI: 10.7554/eLife.78110

      Resource: (Thermo Fisher Scientific Cat# A-11039, RRID:AB_2534096)

      Curator: @scibot

      SciCrunch record: RRID:AB_2534096


      What is this?

    4. RRID:AB_2576217

      DOI: 10.7554/eLife.78110

      Resource: (Thermo Fisher Scientific Cat# A-11034, RRID:AB_2576217)

      Curator: @scibot

      SciCrunch record: RRID:AB_2576217


      What is this?

    5. RRID:AB_10013483

      DOI: 10.7554/eLife.78110

      Resource: (Takara Bio Cat# 632496, RRID:AB_10013483)

      Curator: @scibot

      SciCrunch record: RRID:AB_10013483


      What is this?

    6. RRID:AB_2534023

      DOI: 10.7554/eLife.78110

      Resource: (Thermo Fisher Scientific Cat# A10262, RRID:AB_2534023)

      Curator: @scibot

      SciCrunch record: RRID:AB_2534023


      What is this?

    7. RRID:AB_221569

      DOI: 10.7554/eLife.78110

      Resource: (Molecular Probes Cat# A-11122, RRID:AB_221569)

      Curator: @scibot

      SciCrunch record: RRID:AB_221569


      What is this?

    8. RRID:AB_2314866

      DOI: 10.7554/eLife.78110

      Resource: (DSHB Cat# nc82, RRID:AB_2314866)

      Curator: @scibot

      SciCrunch record: RRID:AB_2314866


      What is this?

    1. Using this method, we show white/grey hairs that naturally regain pigmentation across sex, ethnicities, ages, and body regions, thereby quantitatively defining the reversibility of greying in humans. Molecularly, grey hairs upregulate proteins related to energy metabolism, mitochondria, and antioxidant defenses. Combining HPP profiling and proteomics on single hairs, we also report hair greying and reversal that can occur in parallel with psychological stressors. To generalize these observations, we develop a computational simulation, which suggests a threshold-based mechanism for the temporary reversibility of greying.

      https://elifesciences.org/articles/67437

      Researchers have shown that some types of greying hair are reversible.

  2. May 2022
    1. RRID: 2336883

      DOI: 10.7554/eLife.71845

      Resource: (Takara Bio Cat# 632592, RRID:AB_2336883)

      Curator: @bandrow

      SciCrunch record: RRID:AB_2336883


      What is this?

    2. RRID:AB_143165

      DOI: 10.7554/eLife.71845

      Resource: (Thermo Fisher Scientific Cat# A-11008, RRID:AB_143165)

      Curator: @scibot

      SciCrunch record: RRID:AB_143165


      What is this?

    3. RRID:AB_310177

      DOI: 10.7554/eLife.71845

      Resource: (Millipore Cat# 06-570, RRID:AB_310177)

      Curator: @scibot

      SciCrunch record: RRID:AB_310177


      What is this?

    4. RRID:AB_444313

      DOI: 10.7554/eLife.71845

      Resource: (Abcam Cat# ab18197, RRID:AB_444313)

      Curator: @scibot

      SciCrunch record: RRID:AB_444313


      What is this?

    5. RRID:AB_2535844

      DOI: 10.7554/eLife.71845

      Resource: (Thermo Fisher Scientific Cat# A-21422, RRID:AB_2535844)

      Curator: @scibot

      SciCrunch record: RRID:AB_2535844


      What is this?

  3. Apr 2022
    1. DGRC: ): https://dgrc.bio.indiana.edu/product/View?product=1568

      DOI: 10.7554/eLife.67598

      Resource: RRID:DGRC_1568

      Curator: @bandrow

      SciCrunch record: RRID:DGRC_1568


      What is this?

    1. DANDI: Distributed Archives for Neurophysiology Data Integration

      DOI: 10.7554/eLife.55130

      Resource: Distributed Archives for Neurophysiology Data Integration (RRID:SCR_017571)

      Curator: @bandrow

      SciCrunch record: RRID:SCR_017571


      What is this?

    1. https://gui.dandiarchive.org

      DOI: 10.7554/eLife.61834

      Resource: Distributed Archives for Neurophysiology Data Integration (RRID:SCR_017571)

      Curator: @bandrow

      SciCrunch record: RRID:SCR_017571


      What is this?

  4. Mar 2022
  5. Feb 2022
    1. 2019-10-25

    2. 10.7554/eLife.51230
    3. The coronavirus S-protein mediates receptor binding and fusion of the viral and host cell membranes. In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation but how S-protein function is maintained is not understood. Reported are the X-ray crystal structures of Class III-V RBDs in complex with human aminopeptidase N (hAPN), as well as the electron cryomicroscopy structure of the 229E S-protein. The structures show that common core interactions define the specificity for hAPN and that the peripheral RBD sequence variation is accommodated by loop plasticity. The results provide insight into immune evasion and the cross-species transmission of 229E and related coronaviruses. We also find that the 229E S-protein can expose a portion of its helical core to solvent. This is undoubtedly facilitated by hydrophilic subunit interfaces that we show are conserved among coronaviruses. These interfaces likely play a role in the S-protein conformational changes associated with membrane fusion.
    4. The human coronavirus HCoV-229E S-protein structure and receptor binding
    1. RRID:SCR_002798

      DOI: 10.7554/eLife.35224

      Resource: GraphPad Prism (RRID:SCR_002798)

      Curator: @scibot

      SciCrunch record: RRID:SCR_002798


      What is this?

    2. RRID:SCR_014199

      DOI: 10.7554/eLife.35224

      Resource: Adobe Photoshop (RRID:SCR_014199)

      Curator: @scibot

      SciCrunch record: RRID:SCR_014199


      What is this?

    3. RRID:SCR_002285

      DOI: 10.7554/eLife.35224

      Resource: Fiji (RRID:SCR_002285)

      Curator: @scibot

      SciCrunch record: RRID:SCR_002285


      What is this?

    4. RRID:SCR_002677

      DOI: 10.7554/eLife.35224

      Resource: AxioVision Imaging System (RRID:SCR_002677)

      Curator: @scibot

      SciCrunch record: RRID:SCR_002677


      What is this?

    5. RRID:AB_10679369

      DOI: 10.7554/eLife.35224

      Resource: (Abcam Cat# ab97051, RRID:AB_10679369)

      Curator: @scibot

      SciCrunch record: RRID:AB_10679369


      What is this?

    6. RRID:AB_305564

      DOI: 10.7554/eLife.35224

      Resource: (Abcam Cat# ab6556, RRID:AB_305564)

      Curator: @scibot

      SciCrunch record: RRID:AB_305564


      What is this?

    7. RRID:SCR_014485

      DOI: 10.7554/eLife.35224

      Resource: MaxQuant (RRID:SCR_014485)

      Curator: @scibot

      SciCrunch record: RRID:SCR_014485


      What is this?

    8. RRID:SCR_013731

      DOI: 10.7554/eLife.35224

      Resource: National Xenopus Resource (RRID:SCR_013731)

      Curator: @scibot

      SciCrunch record: RRID:SCR_013731


      What is this?

    1. B2086

      DOI: 10.7554/eLife.02996

      Resource: A Resource Center For Tetrahymena Thermophila (RRID:SCR_008362)

      Curator: @Naa003

      SciCrunch record: RRID:SCR_008362


      What is this?

    2. CU428

      DOI: 10.7554/eLife.02996

      Resource: A Resource Center For Tetrahymena Thermophila (RRID:SCR_008362)

      Curator: @Naa003

      SciCrunch record: RRID:SCR_008362


      What is this?

    3. CU427

      DOI: 10.7554/eLife.02996

      Resource: A Resource Center For Tetrahymena Thermophila (RRID:SCR_008362)

      Curator: @Naa003

      SciCrunch record: RRID:SCR_008362


      What is this?

    4. B2086

      DOI: 10.7554/eLife.02996

      Resource: A Resource Center For Tetrahymena Thermophila (RRID:SCR_008362)

      Curator: @Naa003

      SciCrunch record: RRID:SCR_008362


      What is this?

    5. CU428

      DOI: 10.7554/eLife.02996

      Resource: A Resource Center For Tetrahymena Thermophila (RRID:SCR_008362)

      Curator: @Naa003

      SciCrunch record: RRID:SCR_008362


      What is this?

    6. CU427

      DOI: 10.7554/eLife.02996

      Resource: A Resource Center For Tetrahymena Thermophila (RRID:SCR_008362)

      Curator: @Naa003

      SciCrunch record: RRID:SCR_008362


      What is this?

  6. Jan 2022
    1. RRID:CVCL_C464

      DOI: 10.7554/eLife.69584

      Resource: (ATCC Cat# CRL-4003, RRID:CVCL_C464)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_C464


      What is this?

    2. RRID:AB_141359

      DOI: 10.7554/eLife.69584

      Resource: (Molecular Probes Cat# A-11012, RRID:AB_141359)

      Curator: @scibot

      SciCrunch record: RRID:AB_141359


      What is this?

    3. RRID:AB_2633218

      DOI: 10.7554/eLife.69584

      Resource: (Thermo Fisher Scientific Cat# 720256, RRID:AB_2633218)

      Curator: @scibot

      SciCrunch record: RRID:AB_2633218


      What is this?

    4. RRID:SCR_006786

      DOI: 10.7554/eLife.69584

      Resource: WebGestalt: WEB-based GEne SeT AnaLysis Toolkit (RRID:SCR_006786)

      Curator: @scibot

      SciCrunch record: RRID:SCR_006786


      What is this?

    5. RRID:SCR_016339

      DOI: 10.7554/eLife.69584

      Resource: Monocle2 (RRID:SCR_016339)

      Curator: @scibot

      SciCrunch record: RRID:SCR_016339


      What is this?

    6. RRID:SCR_007322

      DOI: 10.7554/eLife.69584

      Resource: SEURAT (RRID:SCR_007322)

      Curator: @scibot

      SciCrunch record: RRID:SCR_007322


      What is this?

    7. RRID:SCR_011950

      DOI: 10.7554/eLife.69584

      Resource: vegan (RRID:SCR_011950)

      Curator: @scibot

      SciCrunch record: RRID:SCR_011950


      What is this?

    8. RRID:SCR_017254

      DOI: 10.7554/eLife.69584

      Resource: IQ-TREE (RRID:SCR_017254)

      Curator: @scibot

      SciCrunch record: RRID:SCR_017254


      What is this?

    9. RRID:SCR_016582

      DOI: 10.7554/eLife.69584

      Resource: kallisto (RRID:SCR_016582)

      Curator: @scibot

      SciCrunch record: RRID:SCR_016582


      What is this?

    1. Such opportunities and challenges, best illustrated during the pandemic, lie ahead as medicine moves towards the goal of providing evidence-based care to patients no matter where they live. This trajectory also opens up real opportunities for speed, transparency and rich evaluation across peer review in medicine.

      呼吁医学界提供更为快速、透明、评估更为全面的同行评审创造需求

    2. But even as the pandemic has demonstrated the potential for rapid, author-driven publication to democratize access and accelerate research, it has exposed the challenges of this model of scholarly communication

      作者驱动的快速出版 面向大众开放 促进研究

    3. usher

      展示 更快的 更广泛的 更开放的

  7. Dec 2021
    1. plasmid_46757

      DOI: 10.7554/eLife.72345

      Resource: RRID:Addgene_46757

      Curator: @scibot

      SciCrunch record: RRID:Addgene_46757


      What is this?

    2. plasmid_42250

      DOI: 10.7554/eLife.72345

      Resource: RRID:Addgene_42250

      Curator: @scibot

      SciCrunch record: RRID:Addgene_42250


      What is this?

    3. plasmid_122562

      DOI: 10.7554/eLife.72345

      Resource: RRID:Addgene_122562

      Curator: @scibot

      SciCrunch record: RRID:Addgene_122562


      What is this?

    4. plasmid_122563

      DOI: 10.7554/eLife.72345

      Resource: RRID:Addgene_122563

      Curator: @scibot

      SciCrunch record: RRID:Addgene_122563


      What is this?

    5. Plasmid_42

      DOI: 10.7554/eLife.72345

      Resource: RRID:Addgene_42

      Curator: @scibot

      SciCrunch record: RRID:Addgene_42


      What is this?

    6. RRID:ZFIN_ZDB-GENO-180802-1

      DOI: 10.7554/eLife.72345

      Resource: (ZFIN Cat# ZDB-GENO-180802-1,RRID:ZFIN_ZDB-GENO-180802-1)

      Curator: @scibot

      SciCrunch record: RRID:ZFIN_ZDB-GENO-180802-1


      What is this?

    7. RRID:ZFIN_ZDB-GENO-071217-3

      DOI: 10.7554/eLife.72345

      Resource: (ZFIN Cat# ZDB-GENO-071217-3,RRID:ZFIN_ZDB-GENO-071217-3)

      Curator: @scibot

      SciCrunch record: RRID:ZFIN_ZDB-GENO-071217-3


      What is this?

    8. RRID:ZFIN_ZDB-GENO-100504-13

      DOI: 10.7554/eLife.72345

      Resource: (ZFIN Cat# ZDB-GENO-100504-13,RRID:ZFIN_ZDB-GENO-100504-13)

      Curator: @scibot

      SciCrunch record: RRID:ZFIN_ZDB-GENO-100504-13


      What is this?

    9. Plasmid_122

      DOI: 10.7554/eLife.72345

      Resource: RRID:Addgene_122562

      Curator: @Naa003

      SciCrunch record: RRID:Addgene_122562

      Curator comments: pGEM-T Easy-Gbait-hs-Cre


      What is this?

    1. RRID:SCR_002798

      DOI: 10.7554/eLife.62432

      Resource: GraphPad Prism (RRID:SCR_002798)

      Curator: @scibot

      SciCrunch record: RRID:SCR_002798


      What is this?

    2. RRID:SCR_008520

      DOI: 10.7554/eLife.62432

      Resource: FlowJo (RRID:SCR_008520)

      Curator: @scibot

      SciCrunch record: RRID:SCR_008520


      What is this?

    3. RRID:AB_1107619

      DOI: 10.7554/eLife.62432

      Resource: (Bio X Cell Cat# BE0012, RRID:AB_1107619)

      Curator: @scibot

      SciCrunch record: RRID:AB_1107619


      What is this?

    4. RRID:AB_1107636

      DOI: 10.7554/eLife.62432

      Resource: (Bio X Cell Cat# BE0003-1, RRID:AB_1107636)

      Curator: @scibot

      SciCrunch record: RRID:AB_1107636


      What is this?

    5. RRID:AB_1107705

      DOI: 10.7554/eLife.62432

      Resource: (Bio X Cell Cat# BE0043-1, RRID:AB_1107705)

      Curator: @scibot

      SciCrunch record: RRID:AB_1107705


      What is this?

    6. RRID:AB_1107702

      DOI: 10.7554/eLife.62432

      Resource: (Bio X Cell Cat# BE0043, RRID:AB_1107702)

      Curator: @scibot

      SciCrunch record: RRID:AB_1107702


      What is this?

    7. RRID:AB_1107694

      DOI: 10.7554/eLife.62432

      Resource: (Bio X Cell Cat# BE0055, RRID:AB_1107694)

      Curator: @scibot

      SciCrunch record: RRID:AB_1107694


      What is this?

    8. RRID:AB_467135

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 14-0161-86, RRID:AB_467135)

      Curator: @scibot

      SciCrunch record: RRID:AB_467135


      What is this?

    9. RRID:AB_466193

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 12-7311-82, RRID:AB_466193)

      Curator: @scibot

      SciCrunch record: RRID:AB_466193


      What is this?

    10. RRID:AB_465936

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 12-5773-82, RRID:AB_465936)

      Curator: @scibot

      SciCrunch record: RRID:AB_465936


      What is this?

    11. RRID:AB_466199

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 12-7321-82, RRID:AB_466199)

      Curator: @scibot

      SciCrunch record: RRID:AB_466199


      What is this?

    12. RRID:AB_394341

      DOI: 10.7554/eLife.62432

      Resource: (BD Biosciences Cat# 552126, RRID:AB_394341)

      Curator: @scibot

      SciCrunch record: RRID:AB_394341


      What is this?

    13. RRID:AB_2562503

      DOI: 10.7554/eLife.62432

      Resource: (BioLegend Cat# 145406, RRID:AB_2562503)

      Curator: @scibot

      SciCrunch record: RRID:AB_2562503


      What is this?

    14. RRID:AB_1937317

      DOI: 10.7554/eLife.62432

      Resource: (BD Biosciences Cat# 560776, RRID:AB_1937317)

      Curator: @scibot

      SciCrunch record: RRID:AB_1937317


      What is this?

    15. RRID:AB_10611860

      DOI: 10.7554/eLife.62432

      Resource: (BD Biosciences Cat# 561236, RRID:AB_10611860)

      Curator: @scibot

      SciCrunch record: RRID:AB_10611860


      What is this?

    16. RRID:AB_394587

      DOI: 10.7554/eLife.62432

      Resource: (BD Biosciences Cat# 553052, RRID:AB_394587)

      Curator: @scibot

      SciCrunch record: RRID:AB_394587


      What is this?

    17. RRID:AB_1937327

      DOI: 10.7554/eLife.62432

      Resource: (BD Biosciences Cat# 560783, RRID:AB_1937327)

      Curator: @scibot

      SciCrunch record: RRID:AB_1937327


      What is this?

    18. RRID:AB_1937314

      DOI: 10.7554/eLife.62432

      Resource: (BD Biosciences Cat# 560771, RRID:AB_1937314)

      Curator: @scibot

      SciCrunch record: RRID:AB_1937314


      What is this?

    19. RRID:AB_10563910

      DOI: 10.7554/eLife.62432

      Resource: (BD Biosciences Cat# 561226, RRID:AB_10563910)

      Curator: @scibot

      SciCrunch record: RRID:AB_10563910


      What is this?

    20. RRID:AB_1272189

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 48-0453-82, RRID:AB_1272189)

      Curator: @scibot

      SciCrunch record: RRID:AB_1272189


      What is this?

    21. RRID:AB_2573992

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 47-5932-82, RRID:AB_2573992)

      Curator: @scibot

      SciCrunch record: RRID:AB_2573992


      What is this?

    22. RRID:AB_465232

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 11-5321-82, RRID:AB_465232)

      Curator: @scibot

      SciCrunch record: RRID:AB_465232


      What is this?

    23. RRID:AB_469398

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 17-0453-82, RRID:AB_469398)

      Curator: @scibot

      SciCrunch record: RRID:AB_469398


      What is this?

    24. RRID:AB_10671550

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 48-0251-82, RRID:AB_10671550)

      Curator: @scibot

      SciCrunch record: RRID:AB_10671550


      What is this?

    25. RRID:AB_469366

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 17-0251-82, RRID:AB_469366)

      Curator: @scibot

      SciCrunch record: RRID:AB_469366


      What is this?

    26. RRID:AB_464950

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 11-0141-85, RRID:AB_464950)

      Curator: @scibot

      SciCrunch record: RRID:AB_464950


      What is this?

    27. RRID:AB_1582236

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 48-0112-82, RRID:AB_1582236)

      Curator: @scibot

      SciCrunch record: RRID:AB_1582236


      What is this?

    28. RRID:AB_1107004

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 45-0081-82, RRID:AB_1107004)

      Curator: @scibot

      SciCrunch record: RRID:AB_1107004


      What is this?

    29. RRID:AB_1272193

      DOI: 10.7554/eLife.62432

      Resource: (Thermo Fisher Scientific Cat# 48-0032-82, RRID:AB_1272193)

      Curator: @scibot

      SciCrunch record: RRID:AB_1272193


      What is this?

    1. RRID:SCR_002865

      DOI: 10.7554/eLife.70137

      Resource: SPSS (RRID:SCR_002865)

      Curator: @scibot

      SciCrunch record: RRID:SCR_002865


      What is this?

    2. RRID:SCR_002798

      DOI: 10.7554/eLife.70137

      Resource: GraphPad Prism (RRID:SCR_002798)

      Curator: @scibot

      SciCrunch record: RRID:SCR_002798


      What is this?

    3. RRID:SCR_001622

      DOI: 10.7554/eLife.70137

      Resource: MATLAB (RRID:SCR_001622)

      Curator: @scibot

      SciCrunch record: RRID:SCR_001622


      What is this?

    4. RRID:IMSR_JAX:006852

      DOI: 10.7554/eLife.70137

      Resource: (IMSR Cat# JAX_006852,RRID:IMSR_JAX:006852)

      Curator: @scibot

      SciCrunch record: RRID:IMSR_JAX:006852


      What is this?

  8. Nov 2021
    1. it is designed to help institutional leaders reflect on the extent to which their organization can support sustained and values-driven assessment practices, and where they might focus efforts to further evolve them.

      SPACE to support sustained and values-driven assessment practices.

    2. SPACE is a rubric that is composed of two axes

      adaptation to Indonesian institutes

    3. prioritize rankings over their stated goals for diversity, equity, and inclusion

      prioritize rankings over other missions

    4. the metric oversimplification of scholarly achievement distracts academics and institutions

      metric oversimplification >> distractions

    5. Rather, it is due to a growing reliance on proxy measures of research quality in the management of recruitment, promotion, tenure and funding decisions: these proxy measures are widely used because they are convenient, not because they are meaningful. The pursuit of a higher ranking in league tables for universities has also contributed to the problem.

      proxy measures >> convenient but not meaningful

    6. Ghent University in Belgium made headlines in 2019 when it announced a new policy for evaluating faculty that marked a shift away from the 'rat race' of metrics and rankings towards more holistic processes focused on valuing and nurturing talent

      notice >> rat race

  9. Sep 2021
    1. However, the entire repair process for DSBs is notably more efficient in DR than in other organisms; DR is able to restore hundreds of copies of damaged double strands in an hour

      I guess that answers my last question, but raises another question: what allows the repair process to only be most efficient in DR than other organisms, could there be some other important aspect involved in the mechanism?

    2. The first binding between drRecO and ssDNA occurs in the drSSB-free space of ssDNA and generates a heterotrimer of the drSSB-ssDNA-drRecO complex as an intermediate state. The second binding between drRecO and ssDNA induces the dissociation of drSSB from ssDNA by facilitating a conformational change in ssDNA.

      So ultimately from these findings there are two steps involved in a conformational change in the ssDNA and can avoid using ATP?

    3. molecular mechanism of D. radiodurans (DR) RecO (drRecO) in SSB displacement from ssDNA at the single-molecule level. DR is the toughest bacterium known, with outstanding resistance to ionizing radiation and DNA damage-inducing reagents (Blasius et al., 2008; Cox and Battista, 2005; Slade and Radman, 2011), which are the main causes of DSBs, a form of fatal biological damage at the genomic level

      I wonder if a section of the genome that helps encode for this mechanism and trait for resistance to ionizing radiation can be inserted in other organisms or be used to prevent DNA damage from radiation

    4. In summary, our results demonstrate that drRecO binds to ssDNA sequentially using its two binding sites, which gives drRecO the ability to displace drSSB from ssDNA even though the binding affinity of drRecO for ssDNA is two orders of magnitude smaller than that of drSSB.

      It is amazing that cells are capable of performing actions like this and the researchers were able to show this through their results. I would usually assume that a molecule would need a stronger binding affinity to displace another bound protein, so it was interesting to learn how DrRecO can accomplish this with a much lower binding affinity.

    5. RecO is believed to play a key role in this step because RecO is able to interact with SSB, ssDNA, and dsDNA simultaneously

      So (if I'm interpreting this correctly) RecO is thought to be involved in helping to recruit the rest of the RecFOR machinery to the site of the break because it can interact with SSB, ssDNA, and dsDNA even though SSB binds strongly to ssDNA, right?

  10. Aug 2021
    1. RRID:AB_10861977

      DOI: 10.7554/eLife.70564

      Resource: (Abcam Cat# ab113687, RRID:AB_10861977)

      Curator: @scibot

      SciCrunch record: RRID:AB_10861977


      What is this?

    2. RRID:AB_303395

      DOI: 10.7554/eLife.70564

      Resource: (Abcam Cat# ab290, RRID:AB_303395)

      Curator: @scibot

      SciCrunch record: RRID:AB_303395


      What is this?

    1. RRID:Addgene_113005

      DOI: 10.7554/eLife.67294

      Resource: RRID:Addgene_113005

      Curator: @scibot

      SciCrunch record: RRID:Addgene_113005


      What is this?

    2. RRID:AB_10015282

      DOI: 10.7554/eLife.67294

      Resource: (Jackson ImmunoResearch Labs Cat# 711-035-152, RRID:AB_10015282)

      Curator: @scibot

      SciCrunch record: RRID:AB_10015282


      What is this?

    3. RRID:AB_2338133

      DOI: 10.7554/eLife.67294

      Resource: (Jackson ImmunoResearch Labs Cat# 112-035-062, RRID:AB_2338133)

      Curator: @scibot

      SciCrunch record: RRID:AB_2338133


      What is this?

    4. RRID:AB_2338510

      DOI: 10.7554/eLife.67294

      Resource: (Jackson ImmunoResearch Labs Cat# 115-035-164, RRID:AB_2338510)

      Curator: @scibot

      SciCrunch record: RRID:AB_2338510


      What is this?

    5. RRID:AB_2340447

      DOI: 10.7554/eLife.67294

      Resource: (Jackson ImmunoResearch Labs Cat# 706-035-148, RRID:AB_2340447)

      Curator: @scibot

      SciCrunch record: RRID:AB_2340447


      What is this?

    6. RRID:AB_2568986

      DOI: 10.7554/eLife.67294

      Resource: (Nakamura A; Development. 2001 Cat# me31B, RRID:AB_2568986)

      Curator: @scibot

      SciCrunch record: RRID:AB_2568986


      What is this?

    7. RRID:AB_10598029

      DOI: 10.7554/eLife.67294

      Resource: (MBL International Cat# PM013-7, RRID:AB_10598029)

      Curator: @scibot

      SciCrunch record: RRID:AB_10598029


      What is this?

    8. RRID:AB_528189

      DOI: 10.7554/eLife.67294

      Resource: (DSHB Cat# F2F4, RRID:AB_528189)

      Curator: @scibot

      SciCrunch record: RRID:AB_528189


      What is this?

    9. RRID:BDSC_4274

      DOI: 10.7554/eLife.67294

      Resource: (BDSC Cat# 4274,RRID:BDSC_4274)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_4274


      What is this?

    10. RRID:BDSC_6839

      DOI: 10.7554/eLife.67294

      Resource: (BDSC Cat# 6839,RRID:BDSC_6839)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_6839


      What is this?

    11. RRID:BDSC_24163

      DOI: 10.7554/eLife.67294

      Resource: (BDSC Cat# 24163,RRID:BDSC_24163)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_24163


      What is this?

    12. RRID:BDSC_38437

      DOI: 10.7554/eLife.67294

      Resource: (BDSC Cat# 38437,RRID:BDSC_38437)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_38437


      What is this?

    13. RRID:BDSC_3248

      DOI: 10.7554/eLife.67294

      Resource: (BDSC Cat# 3248,RRID:BDSC_3248)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_3248


      What is this?

    14. RRID:DGGR_110658

      DOI: 10.7554/eLife.67294

      Resource: (DGGR Cat# 110658,RRID:DGGR_110658)

      Curator: @scibot

      SciCrunch record: RRID:DGGR_110658


      What is this?

    1. RRID:SCR_002798

      DOI: 10.7554/eLife.68268

      Resource: GraphPad Prism (RRID:SCR_002798)

      Curator: @scibot

      SciCrunch record: RRID:SCR_002798


      What is this?

    2. RRID:BDSC_18896

      DOI: 10.7554/eLife.68268

      Resource: (BDSC Cat# 18896,RRID:BDSC_18896)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_18896


      What is this?

    3. RRID:BDSC_24819

      DOI: 10.7554/eLife.68268

      Resource: (BDSC Cat# 24819,RRID:BDSC_24819)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_24819


      What is this?

    4. RRID:BDSC_56254

      DOI: 10.7554/eLife.68268

      Resource: (BDSC Cat# 56254,RRID:BDSC_56254)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_56254


      What is this?

    5. RRID:BDSC_8765

      DOI: 10.7554/eLife.68268

      Resource: (BDSC Cat# 8765,RRID:BDSC_8765)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_8765


      What is this?

    6. RRID:BDSC_5905

      DOI: 10.7554/eLife.68268

      Resource: (BDSC Cat# 5905,RRID:BDSC_5905)

      Curator: @scibot

      SciCrunch record: RRID:BDSC_5905


      What is this?

    1. RRID:Addgene_12253

      DOI: 10.7554/eLife.64148

      Resource: RRID:Addgene_12253

      Curator: @scibot

      SciCrunch record: RRID:Addgene_12253


      What is this?

    2. RRID:AB_2875675

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 751690, RRID:AB_2875675)

      Curator: @scibot

      SciCrunch record: RRID:AB_2875675


      What is this?

    3. RRID:AB_2875614

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 751621, RRID:AB_2875614)

      Curator: @scibot

      SciCrunch record: RRID:AB_2875614


      What is this?

    4. RRID:AB_394629

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 553108, RRID:AB_394629)

      Curator: @scibot

      SciCrunch record: RRID:AB_394629


      What is this?

    5. RRID:AB_394806

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 553355, RRID:AB_394806)

      Curator: @scibot

      SciCrunch record: RRID:AB_394806


      What is this?

    6. RRID:AB_394774

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 553310, RRID:AB_394774)

      Curator: @scibot

      SciCrunch record: RRID:AB_394774


      What is this?

    7. RRID:AB_394595

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 553062, RRID:AB_394595)

      Curator: @scibot

      SciCrunch record: RRID:AB_394595


      What is this?

    8. RRID:AB_396936

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 557915, RRID:AB_396936)

      Curator: @scibot

      SciCrunch record: RRID:AB_396936


      What is this?

    9. RRID:AB_394618

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 553088, RRID:AB_394618)

      Curator: @scibot

      SciCrunch record: RRID:AB_394618


      What is this?

    10. RRID:AB_394643

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 553127, RRID:AB_394643)

      Curator: @scibot

      SciCrunch record: RRID:AB_394643


      What is this?

    11. RRID:AB_10694494

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 5453, RRID:AB_10694494)

      Curator: @scibot

      SciCrunch record: RRID:AB_10694494


      What is this?

    12. RRID:AB_2228008

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 2764, RRID:AB_2228008)

      Curator: @scibot

      SciCrunch record: RRID:AB_2228008


      What is this?

    13. RRID:AB_2062127

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 9239, RRID:AB_2062127)

      Curator: @scibot

      SciCrunch record: RRID:AB_2062127


      What is this?

    14. RRID:AB_2716685

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 12105, RRID:AB_2716685)

      Curator: @scibot

      SciCrunch record: RRID:AB_2716685


      What is this?

    15. RRID:AB_10167668

      DOI: 10.7554/eLife.64148

      Resource: (Santa Cruz Biotechnology Cat# sc-25778, RRID:AB_10167668)

      Curator: @scibot

      SciCrunch record: RRID:AB_10167668


      What is this?

    16. RRID:AB_11210179

      DOI: 10.7554/eLife.64148

      Resource: (Millipore Cat# 07-178, RRID:AB_11210179)

      Curator: @scibot

      SciCrunch record: RRID:AB_11210179


      What is this?

    17. RRID:AB_626736

      DOI: 10.7554/eLife.64148

      Resource: (Santa Cruz Biotechnology Cat# sc-7382, RRID:AB_626736)

      Curator: @scibot

      SciCrunch record: RRID:AB_626736


      What is this?

    18. RRID:AB_203288

      DOI: 10.7554/eLife.64148

      Resource: (Bethyl Cat# A300-081A, RRID:AB_203288)

      Curator: @scibot

      SciCrunch record: RRID:AB_203288


      What is this?

    19. RRID:AB_2160739

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 9542, RRID:AB_2160739)

      Curator: @scibot

      SciCrunch record: RRID:AB_2160739


      What is this?

    20. RRID:AB_2070042

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 9664, RRID:AB_2070042)

      Curator: @scibot

      SciCrunch record: RRID:AB_2070042


      What is this?

    21. RRID:AB_2798429

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 14220, RRID:AB_2798429)

      Curator: @scibot

      SciCrunch record: RRID:AB_2798429


      What is this?

    22. RRID:AB_2631168

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 13987, RRID:AB_2631168)

      Curator: @scibot

      SciCrunch record: RRID:AB_2631168


      What is this?

    23. RRID:AB_2143306

      DOI: 10.7554/eLife.64148

      Resource: (Bethyl Cat# A300-105A, RRID:AB_2143306)

      Curator: @scibot

      SciCrunch record: RRID:AB_2143306


      What is this?

    24. RRID:AB_2688010

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 14197, RRID:AB_2688010)

      Curator: @scibot

      SciCrunch record: RRID:AB_2688010


      What is this?

    25. RRID:AB_2688009

      DOI: 10.7554/eLife.64148

      Resource: (Cell Signaling Technology Cat# 14689, RRID:AB_2688009)

      Curator: @scibot

      SciCrunch record: RRID:AB_2688009


      What is this?

    26. RRID:CVCL_0258

      DOI: 10.7554/eLife.64148

      Resource: (ECACC Cat# 94042252, RRID:CVCL_0258)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_0258


      What is this?

    27. RRID:CVCL_0093

      DOI: 10.7554/eLife.64148

      Resource: (ATCC Cat# CRL-1873, RRID:CVCL_0093)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_0093


      What is this?

    28. RRID:CVCL_0004

      DOI: 10.7554/eLife.64148

      Resource: (KCB Cat# KCB 90029YJ, RRID:CVCL_0004)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_0004


      What is this?

    29. RRID:SCR_002798

      DOI: 10.7554/eLife.64148

      Resource: GraphPad Prism (RRID:SCR_002798)

      Curator: @scibot

      SciCrunch record: RRID:SCR_002798


      What is this?

    30. RRID:AB_2868885

      DOI: 10.7554/eLife.64148

      Resource: (BD Biosciences Cat# 550475, RRID:AB_2868885)

      Curator: @scibot

      SciCrunch record: RRID:AB_2868885


      What is this?

    31. RRID:Addgene_68343

      DOI: 10.7554/eLife.64148

      Resource: RRID:Addgene_68343

      Curator: @scibot

      SciCrunch record: RRID:Addgene_68343


      What is this?

    32. RRID:SCR_001881

      DOI: 10.7554/eLife.64148

      Resource: DAVID (RRID:SCR_001881)

      Curator: @scibot

      SciCrunch record: RRID:SCR_001881


      What is this?

    33. RRID:SCR_017270

      DOI: 10.7554/eLife.64148

      Resource: ComplexHeatmap (RRID:SCR_017270)

      Curator: @scibot

      SciCrunch record: RRID:SCR_017270


      What is this?

    34. RRID:SCR_001802

      DOI: 10.7554/eLife.64148

      Resource: CASAVA (RRID:SCR_001802)

      Curator: @scibot

      SciCrunch record: RRID:SCR_001802


      What is this?

    35. RRID:AB_2753213

      DOI: 10.7554/eLife.64148

      Resource: (Miltenyi Biotec Cat# 130-091-224, RRID:AB_2753213)

      Curator: @scibot

      SciCrunch record: RRID:AB_2753213


      What is this?

    36. RRID:Addgene_12259

      DOI: 10.7554/eLife.64148

      Resource: RRID:Addgene_12259

      Curator: @scibot

      SciCrunch record: RRID:Addgene_12259


      What is this?

    37. RRID:Addgene_12251

      DOI: 10.7554/eLife.64148

      Resource: RRID:Addgene_12251

      Curator: @scibot

      SciCrunch record: RRID:Addgene_12251


      What is this?

    38. RRID:CVCL_1418

      DOI: 10.7554/eLife.64148

      Resource: (DSMZ Cat# ACC-15, RRID:CVCL_1418)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_1418


      What is this?

    39. RRID:CVCL_0064

      DOI: 10.7554/eLife.64148

      Resource: (DSMZ Cat# ACC-102, RRID:CVCL_0064)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_0064


      What is this?

    40. RRID:CVCL_0006

      DOI: 10.7554/eLife.64148

      Resource: (RCB Cat# RCB1189, RRID:CVCL_0006)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_0006


      What is this?

    41. RRID:CVCL_8227

      DOI: 10.7554/eLife.64148

      Resource: (RRID:CVCL_8227)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_8227


      What is this?

    42. RRID:CVCL_B488

      DOI: 10.7554/eLife.64148

      Resource: (RRID:CVCL_B488)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_B488


      What is this?

    43. RRID:CVCL_UL49

      DOI: 10.7554/eLife.64148

      Resource: (RRID:CVCL_UL49)

      Curator: @scibot

      SciCrunch record: RRID:CVCL_UL49


      What is this?

    44. RRID:Addgene_57822

      DOI: 10.7554/eLife.64148

      Resource: RRID:Addgene_57822

      Curator: @scibot

      SciCrunch record: RRID:Addgene_57822


      What is this?

    1. RRID:SCR_020309

      DOI: 10.7554/eLife.66768

      Resource: Nanodrop 2000c Spectrophotometer (RRID:SCR_020309)

      Curator: @scibot

      SciCrunch record: RRID:SCR_020309


      What is this?

    2. RRID:AB_2536530

      DOI: 10.7554/eLife.66768

      Resource: (Thermo Fisher Scientific Cat# G-21234, RRID:AB_2536530)

      Curator: @scibot

      SciCrunch record: RRID:AB_2536530


      What is this?

    3. RRID:AB_2536527

      DOI: 10.7554/eLife.66768

      Resource: (Thermo Fisher Scientific Cat# G-21040, RRID:AB_2536527)

      Curator: @scibot

      SciCrunch record: RRID:AB_2536527


      What is this?

    4. RRID:AB_262044

      DOI: 10.7554/eLife.66768

      Resource: (Sigma-Aldrich Cat# F1804, RRID:AB_262044)

      Curator: @scibot

      SciCrunch record: RRID:AB_262044


      What is this?

    5. RRID:AB_477583

      DOI: 10.7554/eLife.66768

      Resource: (Sigma-Aldrich Cat# T6199, RRID:AB_477583)

      Curator: @scibot

      SciCrunch record: RRID:AB_477583


      What is this?

    6. RRID:AB_302613

      DOI: 10.7554/eLife.66768

      Resource: (Abcam Cat# ab1791, RRID:AB_302613)

      Curator: @scibot

      SciCrunch record: RRID:AB_302613


      What is this?

    7. RRID:AB_10013427

      DOI: 10.7554/eLife.66768

      Resource: (Takara Bio Cat# 632380, RRID:AB_10013427)

      Curator: @scibot

      SciCrunch record: RRID:AB_10013427


      What is this?

    1. RRID:AB_355445

      DOI: 10.7554/eLife.49818

      Resource: (R and D Systems Cat# AF566, RRID:AB_355445)

      Curator: @scibot

      SciCrunch record: RRID:AB_355445


      What is this?

    2. RRID:AB_2133200

      DOI: 10.7554/eLife.49818

      Resource: (Millipore Cat# MAB5272, RRID:AB_2133200)

      Curator: @scibot

      SciCrunch record: RRID:AB_2133200


      What is this?

    3. RRID:AB_2298772

      DOI: 10.7554/eLife.49818

      Resource: (Millipore Cat# MAB377, RRID:AB_2298772)

      Curator: @scibot

      SciCrunch record: RRID:AB_2298772


      What is this?

    4. RRID:AB_10013382

      DOI: 10.7554/eLife.49818

      Resource: (Agilent Cat# Z0334, RRID:AB_10013382)

      Curator: @scibot

      SciCrunch record: RRID:AB_10013382


      What is this?

    5. RRID:AB_10000240

      DOI: 10.7554/eLife.49818

      Resource: (Aves Labs Cat# GFP-1020, RRID:AB_10000240)

      Curator: @scibot

      SciCrunch record: RRID:AB_10000240


      What is this?

    6. RRID:AB_221569

      DOI: 10.7554/eLife.49818

      Resource: (Molecular Probes Cat# A-11122, RRID:AB_221569)

      Curator: @scibot

      SciCrunch record: RRID:AB_221569


      What is this?

    1. RRID:AB_2314986

      DOI: 10.7554/eLife.49226

      Resource: (J. Sevigny, Laval University; Quebec; Canada Cat# NTPDase2, RRID:AB_2314986)

      Curator: @scibot

      SciCrunch record: RRID:AB_2314986


      What is this?

    1. RRID:SCR_015406

      DOI: 10.7554/eLife.49995

      Resource: University of Pennsylvania Center for Molecular Therapy for Cystic Fibrosis (RRID:SCR_015406)

      Curator: @scibot

      SciCrunch record: RRID:SCR_015406


      What is this?

    2. RRID:SCR_014847

      DOI: 10.7554/eLife.49995

      Resource: Salk Institute Gene Transfer Targeting and Therapeutics Core (GT3) (RRID:SCR_014847)

      Curator: @scibot

      SciCrunch record: RRID:SCR_014847


      What is this?

    3. RRID:SCR_002448

      DOI: 10.7554/eLife.49995

      Resource: UNC Joint Vector Laboratories (RRID:SCR_002448)

      Curator: @scibot

      SciCrunch record: RRID:SCR_002448


      What is this?

    1. RRID:AB_659848

      DOI: 10.7554/eLife.47891

      Resource: (Cell Signaling Technology Cat# 9753, RRID:AB_659848)

      Curator: @scibot

      SciCrunch record: RRID:AB_659848


      What is this?