- Sep 2021
because of its small size, binding of tris-NTA has minimum effect on biochemical and physicochemical properties of the protein
small tag may not alter the conformation and function of the protein
The observed Kd values were 1.3 ± 0.2 nM for the His6 peptide, 0.6 ± 0.3 nM for IDH R132H and 2.4 ± 1.1 nM for p38α
- binding Kd is about 1nM
- Kd for different targets could be slightly different
- Aug 2021
- Mar 2021
Benjamin Franklin's father, Josiah Franklin, was a tallow chandler, soaper, and candlemaker
Franklin came from parents with an ordinary background, and rose to become one of the greatest statesmen, scientist, inventors, and authors of his day.
Fibar bi jàngal na taawan bu góor ni ñuy dagge reeni aloom.
Le guérisseur a appris à son fils aîné comment on coupe les racines du Diospyros.
fibar -- (fibar bi? the healer? as in feebar / fièvre / fever? -- used as a general term for sickness).
bi -- the (indicates nearness).
jàngal v. -- to teach (something to someone), to learn (something from someone) -- compare with jàng (as in janga wolof) and jàngale.
na -- pr. circ. way, defined, distant. How? 'Or' What. function indicator. As.
taaw+an (taaw) bi -- first child, eldest. (taawan -- his eldest).
bu -- the (indicates relativeness).
góor gi -- man; male.
ni -- pr. circ. way, defined, distant. How? 'Or' What. function indicator. As.
ñuy -- they (?).
dagg+e (dagg) v. -- cut; to cut.
reen+i (reen) bi -- root, taproot, support.
aloom gi -- Diospyros mespiliformis, EBENACEA (tree).
Dafa ànd ak moroom yi àll ba, fori aloom.
Il est allé dans la brousse ramasser des fruits de Diospyros avec ses camarades.
dafa -- he/she.
ànd v. / ànd bi -- to be together, to go together; copulate; going together, fellowship; placenta.
ak -- and, with, etc.
moroom mi -- comrade of the same age group, equal, companion, neighbor.
yi -- the (plural).
àll bi -- large expanse of uninhabited land, bush; distant, as opposed to home.
ba -- the (indicates distance).
for+i (for) v. -- to pick up.
aloom bi -- edible fruit of Diospyros mespiliformis (aloom gi for the tree).
Alalu baayam la donn.
Il a hérité des biens de son père.
alal+u (alal) ji -- property, fortune. 💰
baay+am (baay) bi -- dad (his). 👨👦
la -- he (?).
donn v. -- to inherit.
Sëriñ boobu aj na daaw, doomam a ko wuutu léegi.
Ce marabout est décédé l'an dernier, c'est son fils qui le remplace maintenant.
sëriñ bi -- marabout.
boobu -- this.
aj (Arabic: Hajj) v. -- make the pilgrimage to Mecca. 🕋; deceased ☠️ (for a religious personality).
na -- he (?).
daaw n. -- last year. 🗓
doom+am (doom) ji -- child by descent 👶🏽; doll🪆; to have a child.
- pick up
- Dec 2019
Her feet, swinging up and down high in the air, seemed to be nodding at him.
---"not doing the can-can, for God's sake.
“You’re glad to be rid of me, aren’t you?” he said.
- As in Shaw's play, he wants his wives to become something they are not
- Guilt makes him wish his wife was actually pushing him to his love affair
- Again he is trying to get approval for his wrong doings
- He is trying to make excuses to redeem himself for cheating on his wife.
- Aug 2019
Mandy says she would be afraid to go back without some proof that you were disposed to treat us justly and kindly; and we have concluded to test your sincerity by asking you to send us our wages for the time we served you. This will make us forget and forgive old scores, and rely on your justice and friendship in the future. I served you faithfully for thirty-two years, and Mandy twenty years. At twenty-five dollars a month for me, and two dollars a week for Mandy, our earnings would amount to eleven thousand six hundred and eighty dollars. Add to this the interest for the time our wages have been kept back, and deduct what you paid for our clothing, and three doctor’s visits to me, and pulling a tooth for Mandy, and the balance will show what we are in justice entitled to
What do Jourdan and Mandy demand from their former enslaver before agreeing to return
- Aug 2018
He with his whole posteritie must dye, Dye hee or Justice must; unless for him [ 210 ]
We, Indians can't understand how "his whole posteritie must dye"? We believe in the theory of rebirth and therefore have infinite scope to be liberated. Above all one does not suffer due to others fault. Scripture does not contradict reason.
- Oct 2017
Trump noticeably avoided talking about gun control when he was asked about it, saying that he would speak on the matter with the police as a general assembly. In 2000, trump had different standpoint on gun control, saying that he wanted a ban on assault guns. Personally I think background checks should be placed on people with accounts of a 1 or more felonies. And that weapons with a lethality higher than a pistol's should be restricted during a person's review as a pistol is enough for self defense.