Reviewer #1 (Public Review):

In this manuscript, by using simulation, in vitro and in vivo electrophysiology, and behavioral tests, Peng et al. nicely showed a new approach for the treatment of neuropathic pain in mice. They found that terahertz (THz) waves increased Kv conductance and decreased the frequency of action potentials in pyramidal neurons in the ACC region. Behaviorally, terahertz (THz) waves alleviated neuropathic pain in the mouse model. Overall, this is an interesting study. The experimental design is clear, the data is presented well, and the paper is well-written. I have a few suggestions.

(1) The authors provide strong theoretical and experimental evidence for the impact of voltage-gated potassium channels by terahertz wave frequency. However, the modulation of action potential also relies on non-voltage-dependent ion channels. For example, I noticed that the RMP was affected by THz application (Figure 3F) as well. As the RMP is largely regulated by the leak potassium channels (Tandem-pore potassium channels), I would suggest testing whether terahertz wave photons have also any impact on the Kleak channels as well.

(2) The activation curves of the Kv currents in Figure 2h seem to be not well-fitted. I would suggest testing a higher voltage (>100 mV) to collect more data to achieve a better fitting.

(3) In the part of behavior tests, the pain threshold increased after THz application and lasted within 60 mins. I suggest conducting prolonged tests to determine the end of the analgesic effect of terahertz waves.

(4) Regarding in vivo electrophysiological recordings, the post-HFTS recordings were acquired from a time window of up to 20 min. It seems that the HFTS effect lasted for minutes, but this was not tested in vitro where they looked at potassium currents. This long-lasting effect of HFTS is interesting. Can the authors discuss it and its possible mechanisms, or test it in slice electrophysiological experiments?

(5) How did the authors arrange the fiber for HFTS delivery and the electrode for in vivo multi-channel recordings? Providing a schematic illustration in Figure 4 would be useful.

(6) Some grammatical errors should be corrected.

Reviewer #2 (Public Review):

Summary:

In this manuscript, Peng et al., reported that 36THz high-frequency terahertz stimulation (HFTS) can suppress the activity of pyramidal neurons by enhancing the conductance of voltage-gated potassium channel. The authors also demonstrated the effectiveness of using 36THz HFTS for treating neuropathic pain.

Strengths:

The manuscript is well written and the conclusions are supported by robust results. This study highlighted the potential of using 36THz HFTS for neuromodulation.

Weaknesses:

More characterization of HFTS is needed, so the readers can have a better assessment of the potential usage of HFTS in their own applications.

(1) It would be very helpful to estimate the volume of tissue that can be influenced by HFTS. It is not clear how 15 mins HFTS was chosen for this functional study. Does a longer time have a stronger effect? A better characterization of the relationship between the stimulus duration of HFTS and its beneficial effects would be very useful.

(2) How long does the behavioral effect last after 15 minutes of HFTS? Figure 5b only presents the behavioral effect for one hour, but the pain level is still effectively reduced at this time point. The behavioral measurement should last until pain sensitization drops back to pre-stim level.

(3) Although the manuscript only tested in ACC, it will also be useful to demonstrate the neural modulation effect on other brain regions. Would 36THz HFTS also robustly modulate activities in other brain regions? Or are different frequencies needed for different brain regions?

Reviewer #3 (Public Review):

Summary:

This manuscript by Peng et al. presents intriguing data indicating that high-frequency terahertz stimulation (HFTS) of the anterior cingulate cortex (ACC) can alleviate neuropathic pain behaviors in mice. Specifically, the investigators report that terahertz (THz) frequency stimulation widens the selectivity filter of potassium channels thereby increasing potassium conductance and leading to a reduction in the excitability of cortical neurons. In voltage clamp recordings from layer 5 ACC pyramidal neurons in acute brain slice, Peng et al. show that HFTS enhances K current while showing minimal effects on Na current. Current clamp recording analyses show that the spared nerve injury model of neuropathic pain decreases the current threshold for action potential (AP) generation and increases evoked AP frequency in layer 5 ACC pyramidal neurons, which is consistent with previous studies. Data are presented showing that ex-vivo treatment with HFTS in slice reduces these SNI-induced changes to excitability in layer 5 ACC pyramidal neurons. The authors also confirm that HFTS reduces the excitability of layer 5 ACC pyramidal neurons via in vivo multi-channel recordings from SNI mice. Lastly, the authors show that HFTS is effective at reducing mechanical allodynia in SNI using both the von Frey and Catwalk analyses. Overall, there is considerable enthusiasm for the findings presented in this manuscript given the need for non-pharmacological treatments for pain in the clinical setting.

Strengths:

The authors use a multifaceted approach that includes modeling, ex-vivo and in-vivo electrophysiological recordings, and behavioral analyses. Interpretation of the findings is consistent with the data presented. This preclinical work in mice provides new insight into the potential use of directed high-frequency stimulation to the cortex as a primary or adjunctive treatment for chronic pain.

Weaknesses:

There are a few concerns noted that if addressed, would significantly increase enthusiasm for the study.

(1) The left Na current trace for SNI + HFTS in Figure 2B looks to have a significant series resistance error. Time constants (tau) for the rate of activation and inactivation for Na currents would be informative.

(2) It is unclear why an unpaired t-test was performed for paired data in Figure 2. Also, statistical methods and values for non-significant data should be presented.

(3) It would seem logical to perform HFTS on ACC-Pyr neurons in acute slices from sham mice (i.e. Figure 3 scenario). These experiments would be informative given the data presented in Figure 4.

(4) As the data are presented in Figure 4g, it does not seem as if SNI significantly increased the mean firing rate for ACC-Pyr neurons, which is observed in the slice. The data were analyzed using a paired t-test within each group (sham and SNI), but there is no indication that statistical comparisons across groups were performed. If the argument is that HFTS can restore normal activity of ACC-Pyr neurons following SNI, this is a bit concerning if no significant increase in ACC-Pyr activity is observed in in-vivo recordings from SNI mice.

(5) The authors indicate that the effects of HFTS are due to changes in Kv1.2. However, they do not directly test this. A blocking peptide or dendrotoxin could be used in voltage clamp recordings to eliminate Kv1.2 current and then test if this eliminates the effects of HFTS. If K current is completely blocked in VC recordings then the authors can claim that currents they are recording are Kv1.1 or 1.2.

(6) The ACC is implicated in modulating the aversive aspect of pain. It would be interesting to know whether HFTS could induce conditioned place preference in SNI mice via negative reinforcement (i.e. alleviation of spontaneous pain due to the injury). This would strengthen the clinical relevance of using HFTS in treating pain.

eLife assessment

Hou and colleagues describe the the use of a previously characterized FRET sensor for use in determining gamma secretase activity in the brain of living mice. In an approach that targeted the sensor to neurons, they observe patterns of fluorescent sensor readout suggesting clustered regions of secretase activity. These results once validated would be valuable in the field of Alzheimer's Disease research, yet further validation of the approach is required, as the current evidence provided is inadequate to support the conclusions.

Reviewer #1 (Public Review):

Summary:

In their paper, Hou and co-workers explored the use of a FRET sensor for endogenous g-sec activity in vivo in the mouse brain. They used AAV to deliver the sensor to the brain for neuron specific expression and applied NIR in cranial windows to assess FRET activity; optimizing as well an imaging and segmentation protocol. In brief they observe clustered g-sec activity in neighboring cells arguing for a cell non-autonomous regulation of endogenous g-sec activity in vivo.

Weaknesses:

Overall the authors provide a very limited data set and in fact only a proof of concept that their sensor can be applied in vivo. This is not really a research paper, but a technical note. With respect to their observation of clustered activity, the images do not convince me as they show only limited areas of interest: from these examples (for instance fig 5) one sees that merely all neurons in the field show variable activity and a clustering is not really evident from these examples. Even within a cluster, there is variability. With r values between 0.23 to .36, the correlation is not that striking. The authors herein do not control for expression levels of the sensor: for instance, can they show that in all neurons in the field, the sensor is equally expressed, but FRET activity is correlated in sets of neurons? Or are the FRET activities that are measured only in positively transduced neurons, while neighboring neurons are not expressing the sensor? Without such validation, it is difficult to make this conclusion.

Secondly, I am lacking some more physiological relevance for this observation. The experiments are performed in wild-type mice, but it would be more relevant to compare this with a fadPSEN1 KI or a PSEN1cKO model to investigate the contribution of a gain of toxic function or LOF to the claimed cell non-autonomous activations. Or what would be the outcome if the sensor was targeted to glial cells?

For this reviewer it is not clear what resolution they are measuring activity, at cellular or subcellular level? In other words are the intensity spots neuronal cell bodies? Given g-sec activity are in all endosomal compartments and at the cell surface, including in the synapse, does NIR imaging have the resolution to distinguish subcellular or surface localized activities? If cells 'communicate' g-sec activities, I would expect to see hot spots of activity at synapses between neurons: is this possible to assess with the current setup?

Without some more validation and physiological relevant studies, it remains a single observation and rather a technical note paper, instead of a true research paper.

Reviewer #2 (Public Review):

Summary:

The manuscript by Hou et al is a short technical report which details the potential use of a recently developed FRET based biosensor for gamma-secretase activity (Houser et al 2020) for in vivo imaging in the mouse brain. Gamma-secretase plays a crucial role in Alzheimer disease pathology and therefore developing methodologies for precise in vivo measurements would be highly valuable to better understand AD pathophysiology in animal models.

The current version of the sensor utilizes a pair of far-red fluorescent proteins fused to a substrate of the enzyme. Using live imaging, it was previously demonstrated it is possible to monitor gamma-secretase activity in cultured cells. Notably, this is a variant of a biosensor that was previously described using CFP-YFP variants FRET pair (Maesako et al, iScience. 2020). The main claim and hypothesis for the MS is that IR excitation and emission has considerable advantages in terms of depth of penetration, as well as reduction in autofluorescence. These properties would make this approach potentially suitable to monitor cellular level dynamics of Gama-secretase in vivo.

The authors use confocal microscopy and show it is possible to detect fluorescence from single cortical cells. The paper described in detail technical information regarding imaging and analysis. The data presented in figures 5-8 details analysis of FRET ratio (FR) measurements within populations of cells. The authors claim it is possible to obtain reliable measurements at the level of individual cells. They compare the FR values across cells and mice and find a spatial correlation among neighboring cells. This is compared with data obtained after inhibition of endogenous gamma-secretase activity, which abolishes this correlation.

Strengths:

The authors describe in detail their experimental design and analysis for in vivo imaging of the reporter. The idea of using a far-red FRET sensor for in vivo imaging is novel and potentially useful to circumvent many of the pitfalls associated with intensity-based FRET imaging in complex biological environments (such as autofluorescence and scattering).

Weaknesses:

There are several critical points regarding validation of this approach and concerns with the data presented that must be addressed:

(1) Regarding the variability and spatial correlation- the dynamic range of the sensor previously reported in vitro is in the range of 20-30% change (Houser et al 2020) whereas the range of FR detected in vivo is between cells is significantly larger (Fig. 3). This raises considerable doubts for specific detection of cellular activity (see point 3).<br /> (2) One direct way to test the dynamic range of the sensor in vivo, is to increase or decrease endogenous gamma-secretase activity and to ensure this experimental design allows to accurately monitor gamma-secretase activity. In the previous characterization of the reporter (Hauser et al 2020), DAPT application and inhibition of gamma-secretase activity results in increased FR (Figures 2 and 3 of Houser et al). This is in agreement with the design of the biosensor, since FR should be inversely correlated with enzymatic activity. Here, while the authors repeat the same manipulation and apply DAPT to block gamma-secretase activity, it seems to induce the opposite effect and reduces FR (comparing figures 8 with figures 5,6,7). First, there is no quantification comparing FR with and without DAPT. Moreover, it is possible to conduct this experiment in the same animals, meaning comparing FR before and after DAPT in the same mouse and cell populations. This point is absolutely critical- if indeed FR is reduced following DAPT application, this needs to be explained since this contradicts the basic design and interpretation of the biosensor.<br /> (3) For further validation, I would suggest including in vivo measurements with a sensor version with no biological activity as a negative control, for example, a mutation that prevents enzymatic cleavage and FRET changes. This should be used to showcase instrumental variability and would help to validate the variability of FR is indeed biological in origin. This would significantly strengthen the claims regarding spatial correlation within population of cells.<br /> (4) In general, confocal microcopy is not ideal for in vivo imaging. Although the authors demonstrate data collected using IR imaging increases penetration depth, out of focus fluorescence is still evident (Figure 4). Many previous papers have primarily used FLIM based analysis in combination with 2p microscopy for in vivo FRET imaging (Some examples: Ma et al, Neuron, 2018; Massengil et al, Nature methods, 2022; DIaz-Garcia et al, Cell Metabolism, 2017; Laviv et al, Neuron, 2020). This technique does not rely on absolute photon number and therefore has several advantage sin terms of quantification of FRET signals in vivo.<br /> It is therefore likely that use of previously developed sensors of gamma-secretase with conventional FRET pairs, might be better suited for in vivo imaging. This point should be at least discussed as an alternative.

Reviewer #3 (Public Review):

This paper builds on the authors' original development of a near infrared (NIR) FRET sensor by reporting in vivo real-time measurements for gamma-secretase activity in the mouse cortex. The in vivo application of the sensor using state of the art techniques is supported by a clear description and straightforward data, and the project represents significant progress because so few biosensors work in vivo. Notably, the NIR biosensor is detectable to ~ 100 µm depth in the cortex. A minor limitation is that this sensor has a relatively modest ΔF as reported in Houser et al, which is an additional challenge for its use in vivo. Thus, the data is fully dependent on post-capture processing and computational analyses. This can unintentionally introduce biases but is not an insurmountable issue with the proper controls that the authors have performed here.

The observation of gamma-secretase signaling that spreads across cells is potentially quite interesting, but it can be better supported. An alternative interpretation is that there exist pre-formed and clustered hubs of high gamma-secretase activity, and that DAPT has stochastic or differential accessibility to cells within the cluster. This could be resolved by an experiment of induction, for example, if gamma-secretase activity is induced or activated at a specific locale and there was observed coordinated spreading to neighboring neurons with their sensor.

Furthermore, to rule out the possibility that uneven viral transduction was not simply responsible for the observed clustering, it would be helpful to see an analysis of 670nm fluorescence alone.

https://taz.de/CO2-Abscheidung-bei-der-Hannover-Messe/!6003357/

reply to u/bastugubbar at https://www.reddit.com/r/typewriters/comments/1ca8nwk/i_for_one_welcome_our_new_taylor_swift_overlords/

Let's be honest here, the most recent typewriter reference (presumably to that of an ex-boyfriend) is certainly not her first. I'm a modest Swiftie at best (from a trivia perspective), preferring to think of her work as poetry rather than musical pop-culture, so I imagine her more as a quill pen sort of writer, though my notes indicate she does take some of her notes for composition using her cell phone.

This being said, a few years back she did feature a red Sears Cutlass in All Too Well (10 Minute Version) (Taylor's Version) at the 8:28 mark, which hasn't driven the cost of these through the roof, though I have seen one listed for $1,000 (it unsurprisingly didn't sell for that.) For more here see Robert Messenger at OzTypewriter and Ryan Schocket for Buzzfeed. It's not listed anymore, but this past Christmas, she also had a red typewriter Christmas tree ornament in her online store.

Those who were privileged to attend the recent Eras Tour (or see it on Disney+) saw groups of typewriters in the background during several songs.

She's been featuring typewriters for a bit now and it hasn't driven prices through the roof any more than the typewriter renaissance that's been going on for the last few years or so. I suspect that this new round of references isn't going to shift things significantly.

If she does go full-typewriter, which model(s) do you suspect she'd be using amidst the pantheon of other writers? I'd suggest she may be romantic enough to do a late 40's Smith-Corona Clipper... or perhaps while jet-setting a Skyriter?

Type on!

The social model of disability is an approach to disability. The model identifies systemic barriers as the main issues facing people with disabilities. This contrasts with the medical model of disability. The medical model of disability identifies disability as an impairment of the individual.

In this source, the fundamental concept of inclusive design is discussed, including topics such as identifying the issue, addressing inclusive design, history, and other related topics. This site is intriguing to me since it presents numerous instances of inclusive design in our everyday lives that we often overlook, such as playgrounds and parks, text fonts on our phones, and other similar examples.

I read "The Hidden Image Descriptions Making the Internet Accessible" from The New York Times. The article opens with more of an experience than a typical article offer. It puts the reader in the shoes of someone who relies on alt-text Instead of showing photos, it shows alt-text. The article then goes on to explain alt-text, and the issues surrounding how it can be improved. It gives examples of how AI generated alt-text is not always accurate - it showed a photo of pancakes with fruit on top and how the AI generated alt-text said it was a piece of pizza. This can be hard for people who rely on alt-text and cause confusion. It also explains how not many people actually use alt-text to help improve the online experience of those who rely on it.

Hypothesis

Results

I think a very novel point is being made here. The concept of disability is one that is defined by the majority of people in society. If the people we consider disabled were in another society, would they then become ordinary people. We need to think about whether we are putting too much pressure on disabled people.

You are on the verge or falling asleep but you keep yourself awake. Is this a good example?

It seems to me that we have more control over our shaping of behaviors and habits than we have in our actions (free will). So, since we can only process one thought / perform one action at a time, we are training our habitual state of being with each sample.

It might also be the case that this is a way of saving cognitive load and saving energy for something more important at the time. I'd like to use survival as an explanation here.

"we could have made alternative decisions or taken different paths than the ones we actually took."

"we believe that we are the ones actively generating and controlling them. It suggests a sense of agency over our current mental processes and behaviors."

Transparent Peer Review

Download the complete Review Process [PDF] including:

• reviews
• authors' reply
• editorial decisions

</br>

want the links

It is intriguing to consider the possibility that individuals may inadvertently conceal their disability by engaging in masking activities, which may have a negative impact on both their emotional and physical well-being. The fact that individuals modify their conduct in order to conform to the standards and expectations of society, often while hiding their own challenges, is something that is seen and accepted on a regular basis in everyday life. One concern that comes as a result of this conversation is how we may facilitate the creation of surroundings that are more inclusive and do not lay the responsibility of adaptation only on people who have impairments. In order to do this, it is necessary to take into account the ways in which the institutions and attitudes of society may either help or hinder persons with disabilities in terms of their coping mechanism and general well-being.

It is interesting to read about universal design through the lens of user experience design. I am studying to become a teacher so many of my experiences in class have been around accessible design in the classroom (how to design assignments that are accessible and provide students with agency). Before learning about universal design, I never thought about how the design of things impacted others. I feel I am much more aware of design choices and especially ones that restrict those with disabilities. I am grateful that in the future I will be able to design lessons and classroom activities that can be accessible to all students.

similar to - how nature works - physiosphere - the way people think - symbolosphere

reference - https://hyp.is/go?url=https%3A%2F%2Fwww.physics.utoronto.ca%2Fpeople%2Fprofiles%2Frobert-k-logan%2Fsymbolosphere-conceptualiztion-language-and-neo-dualism%2F&group=world

follow up - book - The Turning Point - author Fritjof Capra

from https://hyp.is/rKawpAEpEe-8BWfaUKjlmA/www.linkedin.com/posts/daniel-christian-wahl-51a54616_transformative-education-in-a-crisis-of-activity-7187710848671379456-gCqc/

for - symbolosphere - origins - definition - symbolosphere - definition - physiosphere - definition - neo-dualism - Robert K. Logan - John H. Schumann

origins - symbolosphere - John H. Schumann introduces the complimentary notions of

definition - symbolopshere - the non-physical world of symbolic relationships that includes all its thoughts and communication processes such as language

definition - physiosphere - the physical world, including the human brain.

• This paper introduces these terms in the context of a concept they developed called "neo-dualism",

definition - neo-dualism - a way pragmatic form of dualism that distinguishes mind and brain in the current understanding of neuroscience that is unable to provide an adequate explanation connecting the two.

SOURCE:

Councillors Linda Annis of Surrey, Daniel Fontaine of New Westminster, and Alexa Loo of Richmond, say they will bring motions to their councils that would call on the B.C. NDP government to scrap the three-year experiment that started on Jan. 31, 2023.

My story overlaps a bit with this reading, Its fascinating what stories people have to say about the places they grew up in and lived there.

The first thing that comes to mind is the trading of goods between people and a place where people could offer their services.

for - transition - rapid whole system change - Daniel Christian Wahl

to - https://medium.com/age-of-awareness/transformative-education-in-a-crisis-of-perception-a846075f57e0

for - rapid whole system change - Speed & Scale

summary - hmmm....what's mssing? - They don't explicitly promote citizen led action - They are still using the net zero by 2050 story, - which in many critics eyes is actually far too little and too late - See Kevin Anderson's critique of net zero - https://jonudell.info/h/facet/?max=100&expanded=true&user=stopresetgo&exactTagSearch=true&any=net%2Bzero - They don't address inequality, decolonialization or climate justice issues - They don't identify meta or polycrisis

from - https://hyp.is/J7oIeAEpEe-J1kuOInb20A/www.linkedin.com/posts/colinleduc_we-are-launching-our-speed-scale-2024-global-activity-7188309472837021696-SxSf/

for - rapid whole system change - Speed & Scale - Ryan Panchadsaram

to - https://hyp.is/6DUsiAEnEe-Yr5Ojo_wTSg/speedandscale.com/

Sharing one's experiences with others is a way to help people learn more about you and your background and also lets you reflect on yourself.

Banding students from different parts of the world with different backgrounds helps students learn different perspective.

Based on the situation illustrated here. I wonder how can we , as an educator, do to know more about a student’s family situation instead of asking them or their parents directly. I feel like you have to make a relation with the student first. But I don’t know what to do next.

for - Adverse Childhood Experiences - ACE - Aces Too High - childhood trauma - Intergenerational harm

Summary - A great resource that examines how adverse childhood experiences turns into harmful adult behavior that perpetuates the cycle - Most of the most famous pathological leaders of the modern era, including contemporary ones are examined from the perspective of their ACE

an

?

knew

I wonder if this is true because of the at home audience of the show. I would assume that most viewers are women who have the ability to stay home during the day and watch a daytime television show. This indicates that they likely have the resources to "work on themselves" and make different choices and try new things. The audience is likely not, say, a single, uneducated mother who is working to support her children at one of the jobs that she can get without a degree.

This notion (not just on this particular topic) likely harmed the show and the discussions that could be had on it. I would assume that this meant that only people from marginalized groups who were willing to play this game, and not more radical ones who would really defend themselves as being different and not wanting to fit into 'the norm' would go on the show.

There is something interesting about this inclusion in this article.. It seems as if the authors also can't put themselves in the shoes of the mother, who likely is not proud of what she had to do, but had to get her husband to work so that they could bring in money to live. I understand where they're coming from, but I think that this is unnecessary and sort of goes against the whole point and piles on the already attacked mother.

This seems like the show was using the allusion of being understanding and pushing boundaries to simply parrot the popular belief back to the public while patting themselves on the back for addressing the topic.

It's interesting that the studio audience (I'd assume) has the ability to travel to (likely) another city in the middle of a weekday to attend this taping. It seems as if they have the freedom of either not having children to look after or the financial ability/support system to have other look after them while they go to the studio. This shows how this setup does not work in terms of creating an empathetic space for people to share their stories, as the audience can't put themselves in the shoes of a family where there is one car and a parent who works nights.

I think this is true and I think often times now, theyre just for comedy rather than being forreal.

I wonder if this has changed as we see the government changing and currently what is going on in the world.

I see this as I grew up watching Oprah with my own African American family, and I see that Oprah has influenced many other people from marginalized communities to tune in to her show at the time.

This is especially true considering what is currently going on in America and how other people in other countries would rather deal with what America has going on than whats going on here.

I think that this is especially true considering the fact of how television is a unique way of storytelling that can connect people.

This whole analysis has been quite eye opening to the nature of the show. While the intentions of themes were positive, the reality is that in the process of trying to achieve great, it failed to do good. I think there is no doubt that Oprah Winfrey meant well, however, the way topics were addressed and tackled ended up reinforcing the core problems rather than helping solve them.

This is interesting to read after also watching the "Talk Show Murder" episode. Despite ethics always being a hot point in media, its interesting to see that ethics can technically be violated without much repercussions when the "violation" is done within the perspective of "helping others". It's another case where media can be too intrusive on someone's life and causes people to forget that the people on the show are human too.

I feel like this is a double-edged sword. I think it is a fantastic window for people to view therapy in a less stigmatized way, however, on that same token, it can easily be viewed as a substitute for professional help. On top of that, comparing this version of "therapy" to professional therapy, the cost difference also leans into this idea of "why pay a lot of money for a therapist, when I could get some therapy through my television?"

This is also something I haven't thought about a lot but it intrigues me. Obviously, the intent is to say that discrimination is bad. But the way it is addressed doesn't account for the complexity of the subject. Maybe this is a choice made due to the nature of it being a talk show, and it doesn't have the time to necessarily dive deeper. But I feel like a subject like this would require more than just a "solution" conversation rooted in an incomplete definition.

I feel like this is a good example as to why something like the Fairness Doctrine was revoked. Just because you offer an open voice and free speech for two sides of a conversation, doesn't mean that the overall effects from said conversation couldn't be skewed. Just because two sides can converse, doesn't mean people will agree to either side in a 50/50 manner, so in some cases its better to not say anything rather than to give some air time only to belittle a niche audience.

Including the text highlighted around this annotation, its interesting to see how the Oprah Winfrey Show would push certain social subjects, but then contradict itself by obscuring what contributes to these problems in the first place. I'm wonder if this show were to air today, would this problem be addressed since the vision of the "American Dream" has shifted, or if the same problems would be in place.

This is an interesting statement I never really thought about. But as many of these talk shows strive to represent the "American experience" there are bound to be different interpretations, especially during a time where this concept is changing rapidly.

через api gateway - можно делать, те же запросы к backend. условно он уже отыграет роль proxy. он просто перенаправит (forwarding) на другой url

api gateway - даёт возможность, создавать уже своё api, для взаимодействия со внутреними сервисами yandec cloud

можно просто через это api gateway, создать запрос к другому сервису. apigateWay можно сказать играет роль swagger, он некоторый слой, который помогает именно отправлять запросы куда-то.

Limitations: no consideration of precedence constraints, shared resources, multiprocessors, overhead caused in a mode change

picking the best values ig

oh ok so we're actually investigating this

maybe he's doing averages...

still kinda weird idk

still weird tf

This is why energy is set to 1

Again assuming the WCET is like 5x the actual execution time

This makes sense as you'd expect the utilization of all HI-criticality tasks using C_HI values to be higher than the utilization of all HI-criticality tasks using C_LO values

utilization of all HI-criticality tasks using C_LO values

Ok so we're differing little thingies

Ok but this seems much more accurate so what's tea...

biggest difference along with slowing down speed in urgent mode...why is using actual execution times valid in urgent mode again...?

so randomly choosing a task period in between 1000 and 5000 inclusive

Maybe EAU does this more efficiently than Algorithm A idk

so no power-saving in the urgency mode

So the speeds we just discussed in the example before are not possible aurrrrkkayyy

Ok let's go with that

ok work let's go with that

This seems fake lol

post comment - Linked In SBTi controversy

public support

LOSE-LOSE FOR EVERYONE.

creates hard-to-break cycles and permanently ruins families, with small chance of redemption.

racism

making more things illegal rises crime rates instead of preventing people from doing those things.

proof of racial prejudice

corrupt motives

Nixon blows up these issues to get people on his side

widespread misinformation

beginning of regulation

beginning of drug use

по сути - это спецификая, которая помогает создавать единый стиль restApi. по сути спецификая, как и до этого было написано, удобный элемент для того, чтоб программам, работать с api в написаний документаций

possible evidence of effectiveness

end goal is to help stop drug use

provides home and resources for those in need

proof of efficiency

testimony to the helpfulness

introduction of first hand source

the blind leading the blind honey

So DVFS can't do anything to help save power dissipated by I/O and memory operations

rho is processor speed methinks

Phusion polymerase. Tested on ZymoBIOMICS Microbial Community DNA Standard

Figure out how much this is better by

why ecosystem specific databases vs general ones?

As opposed to a taxonomic assignment

government support

counterargument

evidence

first site

explanation

definition

Sure! Imagine you're building a structure, like a house. Before you start building, you need a plan, right? That's what a schema is in programming – it's like your blueprint.

So, in this code, we're using a tool called Joi to make our blueprint. We want our structure to have a specific type, like a string, and maybe some rules, like a minimum length.

Here's a simple explanation:

1. Constructing the Schema: First, we make our blueprint using Joi. In this case, we're saying we want something called a to be a string. Think of it like saying, "In my house blueprint, I want a room called a, and it should be a string."

javascript const schema = Joi.object({ a: Joi.string() });

1. Adding Rules (Constraints): Now, let's say we want to add a rule to our blueprint, like saying that our room a must be at least 5 characters long. When we add rules, Joi gives us back a new blueprint with that rule added. It's like updating our original blueprint with extra details.

javascript const schemaWithRule = schema.keys({ a: Joi.string().min(5) });

So, in simple terms, we're creating a plan for our data, and then we can add rules to that plan to make sure our data follows certain conditions.

https://factoryinternational.org/factoryplus/editor-in-residence-mckenzie-wark/mckenzie-wark-follow-the-white-rabbit/

interesting article

Poner aquí los signos de DH de acuerdo con el %

A summary of the syntropic agroforestry services this AE website offers.

This webpage offers one person's perspective of SA, and is quite thorough.

This webpage offers one person's perspective of SA, and is quite thorough.

A fresh and more context-driven explanation of syntropic agroforestry.

Lo que se nos presenta en esta visión crítica y consciente en contra de las malas percepciones colonizadas tanto a nivel social como individual, aún vigentes en varios países adoctrinados de creencias y filosofías ajenas, es de vital importancia que estas estrategias de aprendizaje provenientes del pensamiento propio de la epistemología del sur y sus metodologías descolonizadores sean reformadas y puestas en ejecución  cuanto antes, pues estas están diseñadas no solamente para aquellos países que fueron colonizados, sino que estas además están creadas minuciosamente con el fin de ver más allá en los diferentes ámbitos tanto a nivel sociológico, religioso, cultural, político y sobre todo va encaminados directamente en la educación, pues esta es el pilar fundamental para que se presente un cambio favorable, hegemónico, solidario, cultural, más justo e inevitable en un país. 

En el video podemos ver la importancia de los contextos sociales y culturales en el aprendizaje: Las opiniones de Vygotsky y Azobel enfatizan especialmente la importancia de los contextos sociales y culturales en el aprendizaje. Esto sugiere que los educadores deberían considerar factores culturales y sociales al diseñar experiencias de aprendizaje.

DOI: 10.1016/j.celrep.2021.108883

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