26 Matching Annotations
  1. Last 7 days
  2. Nov 2020
    1. Previously, we and others have shown that the inclusion of an antitoxin and the careful balancing of toxin and antitoxin expression levels across relevant conditions can mitigate this effect, resulting in a kill switch that is evolutionarily stable over biologically relevant periods
    1. But using internal api's is dangerous as these may change when you update svelte. If you decide to use this, add a line to your project Readme.md mentioning which internal api's you used and why. Try to write it using other methods when you can.
    1. Some of the verbs implemented by systemctl are designed to provide a high-level overview in a human readable format. All that information is available over dbus, and/or journalctl, systemctl show. We could provide that information in json format, but there's a second problem. Information and format of information printed by e.g. systemctl status is not stable. Since the output is not suitable for programmatic consumption anyway, there's no need to provide it in a machine readable format.
  3. Sep 2020
  4. Aug 2020
    1. Bangaru, S., Ozorowski, G., Turner, H. L., Antanasijevic, A., Huang, D., Wang, X., Torres, J. L., Diedrich, J. K., Tian, J.-H., Portnoff, A. D., Patel, N., Massare, M. J., Yates, J. R., Nemazee, D., Paulson, J. C., Glenn, G., Smith, G., & Ward, A. B. (2020). Structural analysis of full-length SARS-CoV-2 spike protein from an advanced vaccine candidate. BioRxiv, 2020.08.06.234674. https://doi.org/10.1101/2020.08.06.234674

    1. Hsieh, C.-L., Goldsmith, J. A., Schaub, J. M., DiVenere, A. M., Kuo, H.-C., Javanmardi, K., Le, K. C., Wrapp, D., Lee, A. G., Liu, Y., Chou, C.-W., Byrne, P. O., Hjorth, C. K., Johnson, N. V., Ludes-Meyers, J., Nguyen, A. W., Park, J., Wang, N., Amengor, D., … McLellan, J. S. (2020). Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Science. https://doi.org/10.1126/science.abd0826

  5. Jul 2020
  6. Jun 2020
  7. May 2020
  8. Mar 2020
    1. demonstrate that adapting a strain to the intended growth condition increases fitness and in turn improves the stability of the engineered function over hundreds of generations

      Does this imply that an organism taken from a native environment, modified with a plasmid and re-introduced is likely to be stable?

  9. Feb 2020
  10. Dec 2019
    1. a few simple design principles: high expression of genetic circuits comes with the cost of low evolutionary stability, avoid repeated sequences, and the use of inducible promoters increases stability. Inclusion of an antibiotic resistance gene within the circuit does not ensure evolutionary stability.
  11. Nov 2019
  12. Sep 2017
  13. Jul 2017
  14. Jun 2017
    1. CINNA. I am not Cinna the conspirator. FOURTH CITIZEN. It is no matter, his name’s Cinna; pluck but his name out of his heart, and turn him going.

      In this act, mistaken identity is used to break tension. Apart from the obvious comedic relief this scene adds to the ever mounting tension and drama in the play, this scene also indicates the disintegration of society and the lack of social restraints of the general public after Caesar’s death.

      In this scene, the plebeians initially surround Cinna the poet after confusing him with Cinna the conspirator. Even when Cinna repeatedly tells them “I am not Cinna the conspirator”, the citizens, in their bloodthirsty rampage, still decide to kill him, stating that “It is no matter, his name’s Cinna”. This degradation of social standards and the crumbling of the social foundations of Ancient Rome bolster the image of the plebeians as ‘sheep’ to be swayed and controlled by the ruling classes, and solidifies their position in the play.

      It is also no coincidence that Shakespeare made Cinna a poet. In the citizens’ interrogation of Cinna, Cinna not only speaks for himself, but as a poet and as a projection of those in scholarly fields and free speech as a whole. With this, Shakespeare compels the audience to question whom poets and those who provide information to the public are accountable to, and whether free speech is more important than a stable and safe society.

  15. Mar 2017
    1. People that do the activity of parkour are athletic humans capable of controlling their body and using it to the fullest extent. Numerous people hang off of buildings or do handstands with one hand on the edge of tall buildings which show great stability. A person that does a handstand on an edge of a tall building has great strength as well as stability because of their form. Their center of gravity is on the arm that is holding them up, and they are able to stabilize themselves by moving their other arm as well as their legs in a position that "pushes" all the weight onto one spot allowing them to balance.