17 Matching Annotations
  1. Feb 2021
  2. Dec 2020
    1. SOC is added to E. coli cells to aid recovery after exposure to high salt concentrations and heat shock in many chemical-based DNA transformation protocols

      Elbing and Brent, 2002, Green and Sambrook, 2012

    1. For best results, use only LB-Miller agar plates. Do not use LB-Lennox or LB-Luriaplates (Vmax™ growth will be suboptimal).
  3. Oct 2020
    1. Human evolution produced gossip. Cultural anthropology sees gossip as an informal way of enforcing group norms. It is effective in small groups.

      Gossip evolved as a strategy to enforce group norms and it is effective in small groups.

  4. Sep 2020
    1. Nutrient Agar is a general purpose, nutrient medium used for the cultivation of microbes supporting growth of a wide range of non-fastidious organisms.
    1. Different nutrient media were used for selective cultivation of bacteria: kanamycin esculin azide (KAA, Merck Eurolab, Darmstadt, Germany) for enterococci, and Chromocult® agar (Merck) as a selective agent for Enterobacteriaceae. For the heterotrophic plate count the bacterial dilutions were plated on R2A agar
    1. R-2A Agar is formulated as per Reasoner and Geldreich (5). Stressed or injured organismsduring water treatment are unable to grow on high nutrient media, since the faster growing organisms outgrow the former(2). Therefore the use of a low nutrient medium like R-2A Agar incubated for longer incubation periods allows these stressedorganisms to grow well.
    1. MacConkey agar is selective for Gram-negative organisms and helps to differentiate lactose fermenting gram-negative rods from non-lactose fermenting gram-negative rods. It is primarily used for the detection and isolation of members of family enterobacteriaceae and Pseudomonas spp.
  5. May 2020
    1. In medieval learned cultures (all the material in this volume was producedin learned, even academic circles for purposes of reading and new compo-sition), such a thorough mixing of media, especially the visual and the ver-bal, was commonplace

      This sounds much more like the "learned" world in the modern era using multi-media on the internet.

  6. Jan 2019
  7. Feb 2018
    1. While it’s foolish to assign coherent political meaning to Rodriguez’s film, it cannot be denied that the machete is a powerful symbol of violent, popular revolt, a tool/weapon freighted with centuries of significance.

      Media shapes culture, culture shapes society, and society can shape politics. It is cultural examples such as these that really show how influential media can be. Especially in movies that glorify tools to be weapons. Haltman states that there are metaphysical aspect that embody culture.

  8. Apr 2016
    1. massmediarefers to those means of transmission

      When I ask students to post on Youth Voices, I'm asking them to participate in mass media. It's a big jump for some who do very little by friend-to-friend communication.

  9. Sep 2015