4,703 Matching Annotations
  1. Oct 2019
    1. Notice that the Child component class has no state field. The component object created by the custom JSX tag has values of the JSX tag properties. If you define a state field in the component, your state field overwrites the JSX properties.

      no state field

    1. The FBI said it has stopped using the "Black Identity Extremist" tag and acknowledged that white supremacist violence is the biggest terrorist threat this country faces. https://trib.al/OepGw2S

      I love the way she looks because it's like she saying..."wow what took you sooooo long." The Root uses data report from California State University, San Bernardino. The 137-page research helps support the allegations that White Supremacy has been a threat to national security for many years.

      https://www.theroot.com/the-fbi-admits-black-lives-matter-was-never-a-threat-i-1835417043?utm_medium=socialflow&utm_source=theroot_twitter

      https://antisemitism.org.il/wp-content/uploads/2019/08/CSHE-2019-Report-to-the-Nation-FINAL-7.29.19-11-PM.pdf

  2. Sep 2019
    1. use the REPOSITORY:TAG combination rather than IMAGE ID

      Error response from daemon: conflict: unable to delete c565603bc87f (cannot be forced) - image has dependent child images

      I really feel like this should be the accepted answer here but it does depend on the root cause of the problem. When you create a tag it creates a dependency and thus you have to delete the tag and the image in that order. If you delete the image by using the tag rather than the id then you are effectively doing just that.

    1. 5) The selective function. This refers not to human choice at all but to Darwin's theory of natural selection as applied to what he called "the favored races." In short, the idea is to help things along by consciously attempting to improve the breeding stock. Schools are meant to tag the unfit - with poor grades, remedial placement, and other punishments - clearly enough that their peers will accept them as inferior and effectively bar them from the reproductive sweepstakes. That's what all those little humiliations from first grade onward were intended to do: wash the dirt down the drain.

      werd

    1. "Occupation: At Home."a Noone claimed that she was "Poet" or "Writer."

      How completely infuriating, and disrespectful to the legacy of one of the world's most brilliant writers. It's incredible how badly society wants to hold on to this image of Dickinson as an outcast / recluse instead of recognizing her brilliance; the fact that her sister in law had to tag on some information about Dickinson's achievements and intellect (almost as an afterthought) is really unfortunate.

    1. Agregát popisuje slovní tvar, který zastupuje dva nebo více slovních tvarů (složek agregátu) a většinou mu není možné přiřadit jednoduše slovní druh.

      Domníváme se se Sašou, že by bylo žádoucí definovat v rámci NovaMorf u agregátů i (možné) dělení ortografických slov na syntaktická slova. Ortografické slovo by zůstalo jako "hlavní", ale jasně se řekne, že pro účely syntaktického zpracování lze agregát rozdělit na syntaktická slova, odpovídající níže v kapitole uvedeným kombinacím lemma+tag: naň: na|ň očs: o|č|s dělals: dělal|s abyste: a|byste kdybychom: kdy|bychom. U "abyste", "kdybychom" je to poněkud nepřirozené, ale asi nejlepší řešení. Nežádáme "interpretované tokeny" jako v UD.

    1. show only
      1. The typeface 'Avenir Roman' should be used.
      2. The premium tag should be right aligned to the container below.
      3. The premium tag's rectangle should be of dimensions 81x22px.
      4. The check box used is not the same one.
    1. we injected Cre recombinase–inducible adeno-associated viruses (AAV) expressing the optogenetic channelrhodopsin-like ChIEF fused with a tdTomato reporter [AAVdj-CAG-DIO-ChIEF-tdTomato (driven by the CAG promoter) (10, 11)] bilaterally into the rostral ZI of vesicular GABA transporter (VGAT)–Cre mice that express Cre recombinase in GABA neurons

      To target a neuron population of interest, e.g. those that express GABA, scientists use genetically modified viruses (AAVs) to deliver proteins into the brain (such as optogenetic tools).

      This is achieved by using two tools: 1) a mouse line that expressed the enzyme Cre recombinase in a specific population of neurons (e.g. those that express the GABA transporter VGAT) and 2) an AAV that expresses an optogenetic protein only in the presence of Cre. The AAV is injected into the brain region of interest in the Cre mice. This AAV has a tdTomato tag which allows the injection site to be visualized under a fluorescent microscope.

      For further information on these tools see how mice optogenetics are used this video.

      The ZI in both hemispheres of the brain was injected with the AAV (bilaterally), with the region lying towards the front of the brain (rostral) being targeted. The optogenetic tool used (ChIEF) activates neurons when blue light is shone on the cells.

    1. The Path-Goal model emphasizes the importance of the leader’s ability to interpret follower’s needs accurately and to respond flexibly to the requirements of a situation.

      https://kurtstuke.com/Bijoux.jpg

      I had hard time as well. Here is the link. Hope it works.

      Reference

      Free online word cloud generator and tag cloud creator. (2019). Retrieved 5 September 2019, from https://www.wordclouds.com/

      Russell, R., & Gregory Stone, A. (2002). A review of servant leadership attributes: developing a practical model. Leadership & Organization Development Journal, 23(3), 145-157. doi: 10.1108/01437730210424

    1. an iPhone was out of reach for even the richest man on earth.

      I'm not sure I get this. The first IPhone was released with a price tag of $499.00, which, with inflation calculated in, was $617.48. The richest man in 2007 was Bill Gates, who had $62.29 billion. He could literally buy over 100 million IPhones.

    1. September 1939 aus Wehrmacht-Perspektive Deutsche Soldaten fotografierten vom ersten Tag an, wie sie den Beginn des Zweiten Weltkriegs erlebten - und welches Leid sie über ihr Nachbarland brachten. Das Berliner "Haus der Wannsee-Konferenz" zeigt auf seiner Website Fotos des Hauptmanns Kurt Seeliger. Seine Bilder und die anderer Soldaten aus dem Projekt "Stumme Zeugnisse 1939" lassen erahnen, wie stark der Vernichtungswille oft war.

      Drei Männer stehen vor Häuser. Die Häuser sind verbrannt weil die Zweiter Weltkrieg begann hat. Meiner Meinung nach ist es sehr interessant eine Foto von 1939 zu sehen.

  3. Aug 2019
    1. crowd

      An English Sonnet/Elizabethan Sonnet is a type of sonnet with 3 quatrains and 1 couplet (usually a capping couplet). The rhyme scheme is abab cdcd efef gg

    1. No, I’m not very good in school. This is my second year in the seventh grade, and I’m bigger and taller than the other kids. They like me all right, though, even if I don’t say much in the classroom, because outside I can tell them how to do a lot of things. They tag me around and that sort of makes up for what goes on in school.

      This definitely relates to today's situation in American education. He has many different interests and talents, but none of them are being put to use because of the curriculum of schools. The subjects in school relate to his situation at home in some cases. But the teachers only have one way to teach the students, and that's why students' who can't learn that way become bored, and lose desire to learn.

    1. Self-driving cars would not merely represent a private luxury

      But it would be though. Currently, having autonomous driving abilities in your car, adds about $100,000 to the price tag, according to Esurance. Semi-autonomous features would add between $5,000-$10,000 to the base vehicle cost. Where there is a way to profit off something, it'll be made more and more expensive, with fully autonomous vehicles not being available to the general public, due to the cost of producing self-driving cars.

    1. Another field where XSL is expected to beimportant is print XML data with layoutand output it to PDF.For example, when we convert form datainto XML, it is natural to input data onbrowser form and specify the data format isXML, output to paper by specifying XMLformat. Therefore, XML is a tag mappedform, it is necessary for men to understandthe contents and confirm XML to print bygiving format type, or to output digitalizedpaper such as PDF.

      example

    1. 15 Mutace

      Rozumím-li tomu správně, nemohou být mutace (na rozdíl od všech výše uvedených gram. kategorií) pojaty jako jeden znak v pozičním tagsetu. Bylo by asi vhodné popsat, jak se s tím v tagsetu vyrovnat, zda navrhujete, aby byly v pozičním tagsetu hodnoty mutací uvedeny samostatně mimo tag, nebo máte nějaké jiné řešení...

    1. Update the P and I values using the values recorded on the blue tag that shipped with the module.

      I don't think this information is on the blue tag but I could be wrong.

  4. Jul 2019
    1. Your teacher may require you to use tags for a variety of reasons.

      Tags would also be a great way to practice metacognition! One technique would be to have a list of categories you use when annotating (such as "Evidence", "Central Idea", and "Vocab") and apply one of these as a tag to each annotation.

      The goal is to go beyond analyzing the text and identify why you're analyzing that part of the text. You can read more about metacognition here.

    2. Highlights are private, visible only to you when you’re logged in (even if they are made in the Public layer).

      Adding a tag or note appears to convert a highlight to a full-fledged annotation in that you may then choose Post to Public (or to group visibility). Without these, Post to Only Me is the only available option for bare highlights.

    1. locate the blue tag that shipped with the replacement centrifuge

      Perhaps this would be better to state after removing the new centrifuge from the box?

    1. I was viewed, initially, as a tag-along in this process, not an equal partner.

      Pregnant mother = two lives, partner = one lift, no partner can ever expect to be “equal”

    1. In Python, every object has a unique identification tag. Likewise, there is a built-in function that can be called on any object to return its unique id. The function is appropriately called id and takes a single parameter, the object that you are interested in knowing about. You can see in the example below that a real id is usually a very large integer value (corresponding to an address in memory).
    1. ear again. Sometimes he would come up unexpectedly on the opposite side of me, having apparently passed directly und

      It's as if they were playing a game of tag, and Thoreau was horribly under qualified.

    1. It is very important to keep the differences in mind between head, header element, and heading element.

      • The head goes at the very top of the file and is not visible on the page. It is the section where the title tag as well as external files such as CSS files go.

      • The header element is used at the top but inside the body element. It is visible on the page. It is most often used once but some pages include separate headers.

      • The heading element is used to create titles that identify the content or purpose of a section of a page like h1, h2, and so on.
    1. By Product

      if your header is in other non-western language, you need to set id tag. For example:

      第二節

      should be

      第二節{#section2}

      This is because tab's hyperlink can not take Chinese characters, you have to specify its non-Chinese link name yourself.

    1. A class examining and exploring a series of online texts (websites, blogs, wikis) to aid in comprehension and synthesis. Hypothesis allows for a common tag to organize readings across a group.

      Sounds exciting! Looking forward to seeing my classmates annotations!

    1. Nikkei Asian Review

      Hello, I am the Citerpress bot :) I think this sentence is mentioning a news article without an explicit link. I looked in my news database and here is what I found:

      Hit #1 (score of 39.0)

      Hit #2 (score of 37.9)

      Hit #3 (score of 37.2)

      Hit #4 (score of 34.3)

      I did my best! My annotations will get better and better with time, as I index new pages every day.

    1. Manywho cannot afford half the items listed above include themselves in thatdefinition. Also included are most of the nation’s millionaires,who consider themselves middle or upper-middle class despite their obviouslyoutsized income11and their small representation (3.3%) among the country’s overall population.

      Everyone thinks they're middle class!!!

      Also -- wealthy are uneasy about being labeled as such.

      https://www.cnbc.com/2015/05/06/naires-say-theyre-middle-class.html

      https://nypost.com/2017/08/20/why-rich-new-yorkers-are-hiding-their-wealth-and-privilege/

      https://www.amazon.com/Uneasy-Street-Anxieties-Rachel-Sherman/dp/0691165505/ref=sr_1_1?ie=UTF8&qid=1503090665&sr=8-1&keywords=Uneasy+Street%3A+The+Anxieties+of+Affluence&tag=nypost-20

  5. Jun 2019
    1. Aufwühlende Zeiten sind das für Annegret Kramp-Karrenbauer. Als die auch AKK genannte CDU-Bundesvorsitzende am Montagabend beim Ständehaustreff, einer Live-Talkshow der „Rheinischen Post“, in Düsseldorf auf die Bühne kommt, hat sie einen langen Tag mit Gremiensitzungen in Berlin mit vielen wichtigen Klärungen hinter sich.

      "These are disturbing times for Annagret Kramp-Karrenbauer. On monday evening, after a long day of committee meetings in Berlin with important clarifications, she sat down on Ständehaustreff, a live talk show hosted by the Rhenish Post in Berlin.

    1. The FBI said it has stopped using the "Black Identity Extremist" tag and acknowledged that white supremacist violence is the biggest terrorist threat this country faces.

      I am not surprised when the article stated, that white supremacist violence is the biggest terrorist threat that this country faces.

    1. Hi everyone! Thanks for opening the PDF of the evidence for the case study! My hope is that everyone will be able to tag/highlight between 3-5 important pieces of evidence using hypothesis. I know that 185 pages is a lot to go through, so I highlighted a couple of important places for you all to look at for evidence to make it easier for you.

      While looking at the evidence, please look for the following information:

      -Highlight points where Conrad Roy III shows warning signs, risk factors, or protective factors. -Highlight points where Michelle Carter attempted to help Conrad Roy. -Highlight points where you would have said or done something differently than Michelle Carter did.

    Annotators

    1. tag or mention district schools or communities.

      I think that this is something important to highlight as well. There are a lot of people that are looking at a district from online. If people are posting wrong or offensive things about the district, this could create potential problems for people who are looking at the district. I think the true image of the district should be able to be reflected, and not false allegations or posts from kids on social media. Therefore, monitoring the public posts per the districts own well being is important.

    1. Si les réponses ne peuvent être données et rédigées en live j'ai pour habitude de créer et placer un tag "Attente spécifications" sur les US incriminées. Une seconde relecture permettra de l'en enlever une fois tous les doutes levés.

      Il est également possible d'utiliser le statut "Blocked" plus visible (pastille rouge)

    1. Thus, you could use a spreadsheet program to create bar charts of the count of documents with an ‘architecture’ tag or a ‘union history’ tag, or ‘children’, ‘women’, ‘agriculture,’ etc. We might wonder how plaques concerned with ‘children’, ‘women’, ‘agriculture’, ‘industry’, etc might be grouped, so we could use Overview’s search function to identify these plaques by search for a word or phrase, and applying that word or phrase as a tag to everything that is found. One could then visually explore the way various tags correspond with particular folders of similar documents.[5]
  6. May 2019
    1. Then we accept UDP traffic if the value of the udpserver tag is 1 when both sender and receiver tags are ORed together, or if UDP traffic is multicast. This allows multicast mDNS and Netbios announcements and allows UDP traffic to and from UDP servers, but prohibits other horizontal UDP traffic.

      is this the statement indicating MDNS support in ZeroTier?

    1. If innerHTML inserts a <script> tag into the document – it doesn’t execute. It becomes a part of HTML, just as a script that has already run.

      key point!

    1. Menu items are links <a>, not buttons. There are several benefits, for instance: Many people like to use “right click” – “open in a new window”. If we use <button> or <span>, that doesn’t work. Search engines follow <a href="..."> links while indexing.

      key point on why use 'a' tag instead of buttons for navigation purposes

    1. They’ve learned, and that’s more dangerous than caring, because that means they’re rationally pricing these harms. The day that 20% of consumers put a price tag on privacy, freemium is over and privacy is back.

      Google want you to say yes, not because they're inviting positivity more than ever, but because they want you to purchase things and make them richer. This is the essence of capitalism.

    1. Suppose college tuition was free and every first-year had a guaranteed job lined up for after graduation. This parallel universe does exist at military-service academies—and at West Point, Annapolis, and Colorado Springs, humanities majors are at about the same level as they were in 2008.

      Huge clue re: precarity's effect. Also a bit of a portent in the possible effect of a Universal Basic Income. Which has to now become a new tag I use...

    1. yes there are many threads

      Yes. Every time one of these accounts appears, I tag it as a throwaway account. I am doing that will all immediately registered accounts in those threads, no matter who they look like. This allows later analysis, because, without this, the accounts become [deleted] and cannot then be correlated. Interesting patterns appear when this is done. Sometimes it becomes possible to correlate accounts across multiple platforms.

    1. vector, under the phage T7 promoter, in BL21 (DE3) cells, and under the T5 phage promoter, in the pQE30 vector for expression in SG13009[pREP4] and M15[pREP4] cell strains. For cloning in pRSET B, the full length bZP3 initially subcloned in the pBacPAK8 vector at the Kpn I and Sac I sites was released after digestion with Kpn I and EcoR I and cloned in a similarly restricted pRSETB vector inframe with an N-terminal His6 tag. For cloning in the pQE30 vector, the pBacPAK8 carrying the full length bZP3 was initially digested with Not I, filled in with Klenow and then digested with Kpn I. The purified bZP3 fragment was then cloned in the vector digested with Kpn I and Sma I in frame with an N-terminal His6 tag. Though transformants positive for the bZP3 insert in the right reading frame were recovered, no expression could be detected by SDS-PAGE or immunoblots in either case. An alternate strategy was then devised in which an internal fragment of the gene, excluding the signal sequence and the transmembrane-like domain, following the putative furin cleavage site, was amplified by PCR using the forward primer 5'-CGGGATCCCAACCCTTCTGGCTCTTG-3' incorporating a BamH I site and the reverse primer 5'-CCGAGCTCAGAAGCAGACCTGGACCA-3' incorporating a Sac I site. The PCR was done in a 50 J!l volume using 50 pM of each primer and Vent polymerase for extension. The pBluescript-bZP3 (1 0 ng) having a full length bZP3 insert was used as the template and was initially denatured at 95°C for 10 min. Amplification was carried out for 35 cycles of denaturation at 95°C for 2 min, primer annealing at 600C for 2 min and extension at 72°C for 3 min followed by a final extension at 72oc .for 15 min. The amplified bZP3 fragment was digested with BamH I and Sac I and cloned in frame downstream of a His6 tag under the T5 promoter-lac operator control in the pQE30 vector. The authenticity of the construct was confirmed by N-terminal sequencing using an upstream sequencing primer GGCGT ATCACGAGGCCCTTTCG.
    1. argP+, argPd-S94L, argPd-P108S, argPd-P274Sfragment downstream of the phage T7-promoter, such that the encoded proteins beara C-terminal His6-tag provided by the vector DNA sequence. Theresultant plasmid was transformed into strain BL21(DE3) which has the T7 RNA Polymerase under the isopropyl thio-β-D-galactoside (IPTG) inducible lacUV5promoter.The resultant strains were grownin LB (500-1000 ml) to an A600of around 0.6and were then induced with 1 mM IPTG and harvested after 4-hrs of induction.Bacterial cells were recovered by centrifugation, resuspended in 20 ml of lysis buffer(20 mM Tris-Cl, pH-8; 300 mM NaCl; 10 mM DTT and 10 mM imidazole) containing20 μg/ml lysozyme, and lysed by sonication with 30-sec pulses for 10-min. Theprotocol for His6-ArgP(ArgPds)protein purification involved (i) passing the lysate through a 5ml Ni-NTA (Qiagen) chromatographic columnequilibrated with lysis buffer, (ii) washing thecolumn with 100 ml of washing buffer (20 mM Tris-Cl, pH-8; 300 mM NaCl; 10 mMDTT; 30 mM imidazole), and (iii) elution of His6-ArgP(ArgPds)from the column with elutionbuffer (20 mM Tris-Cl, pH-8;300 mM NaCl; 10 mM DTT and 250 mM imidazole) andcollection of 1.5 ml eluate fractions (10 fractions). The fractions were tested forprotein by Bradford method and the protein-carrying fractions (generally tubes 2 to 5)were pooled and dialysed in a 1:200 volume ratio against 20 mM Tris-Cl, pH-8 with 10mM DTT, 300 mMNaCl for 5 hrs followedby a change to buffer of composition 20 mM Tris-Cl, pH-8 with 10 mM DTT, 300 mM NaCl and 40% glycerol for 24 hrs. The proteins were concentrated by centrifugation toaround 1 mg/ml by using Amicon filter (pore size 10-KDa) and stored at −20ºC or −70ºC
    1. The TEI's adoption as a model in digital library projects raised some interesting issues about the whole philosophy of the TEI, which had been designed mostly by scholars who wanted to be as flexible as possible. Any TEI tag can be redefined and tags can be added where appropriate

      Question - What were some of the issues that arose with the TEI's adoption as a model in digital library projects?

      The ability to add tags where needed throughout the texts seems to be a positive aspect due to the added ease of searching key words and it being included in said search.

    1. After 2-3 h exposure,phosphorimagerscreenwas scannedon Fuji FLA-9000 to acquire hybridization images. Next,signal intensity for each spot on the membrane for both input and outputsampleswas quantifiedusing Fuji Multi Gauge V3.0 software andpercentage intensity foreach spot relative tothe whole signal intensity ofthe membranewas determined.To identify mutants with altered survival profiles,ratio of output (Op) to input (Ip) signal for each spot (oligonucleotide tag)present on the membranewas calculated.Mutantsdisplaying at least 6-fold higher and 10-fold lower survival were selectedas “up’ (Op/Ip= 6.0) and ‘down’ (Op/Ip = 0.1) mutants, respectively
    2. 24 h post infection, THP-1 macrophages were washed thrice with PBS, lysed in water and recovered yeast cells were used to infect THP-1 cells at a MOIof 1:10. Three rounds of macrophage infection foreach mutant pool were carried out to enrich for the desired mutants in the final population. The lysate of 3rdround infection was inoculated in YPD medium for overnight (output). Cells were harvested, genomic DNA isolated from each input and output cell pellet andunique signature tags were PCR-amplified with P32-labeledα-dCTP using primers complementary to theinvariant region flanking each unique tag sequence. LabeledPCR products were denatured at 95°C for 10 min, chilled on ice and were hybridized tonylon membranescarrying immobilized plasmid DNA containing 96 unique tagsfor 14-16 h at 42°C.Membranes were washed twicewith 0.1X SSC bufferand exposed to phosphorimager screen for 2-4 h. Radioactive counts for each spot were quantified using Image Quant and Fuji Multi Gauge V3.0 software. Relative percentage intensity for individual spot was calculated with respect to allspots present oneach hybridizedmembrane
    3. YPD-grown cultures (0.05 OD600) of each mutant pool (96 mutants, each carrying a unique signature tag) were either inoculated in YPD medium for overnight (input) or used to infect differentiated THP-1 cells (1X106). After 2 h incubation, non-cell-associated yeastcellswere removed by washing THP-1 cellsthricewith PBS. At
    1. Afteractivating Hypothes.is in the browser, a learner can annotate any piece of text on a webpage with her own ideas, to whichother learners could respond (Principle 1). Tags can be attached to a Hypothes.is annotation, enabling the aggregation of webannotations that are scattered across webpages through a tag (Principle 2). Because Hypothes.is adheres to the Open AnnotationModel, annotations are uniquely identifiable and retrievable, allowing portability of ideas not widely supported in threadeddiscussion forums (Principle 2). The search functionality of Hypothes.is offers means to aggregate annotations according to avariety of criteria such as by tags, users, user groups, and annotated web URLs. Users are thus able to enter the discourse notonly from a specific webpage (e.g., the textbook), but also from the search page that provides another view of the discourse(Principle 3)

      Alignment statement for Hypo

    1. The following antibodies were used in the present study:Primary antibodies against GAPDH (anti-rabbit), FLAG (anti-mouse), Immunoglobulin (IgG, anti-rabbit or anti-mouse),profilin-1 (anti-rabbit), tubulin (anti-mouse) and ubiquitin (anti-rabbit) were obtained from Sigma Aldrich Chemicals(St Louis, MO, USA). Antibodies againstAKT (anti-rabbit), cleaved caspases-3, 8 and 9 (anti-rabbit),HA-tag(anti-rabbit), Myc-tag (anti-rabbit), p21 (anti-rabbit), phospho-p53 (anti-mouse), PTEN (anti-mouse), phospho-AKT (Ser473; anti-rabbit), phospho-GSK-3β (Ser9; anti-rabbit), phospho-IKKα/β (Ser177/181; anti-rabbit), phospho-IκBα (Ser32; anti-rabbit), and phospho-p65 (Ser276; anti-rabbit) were obtained from Cell Signaling Technologies(Danvers, MA, USA), whereas antibodies for cox-2 (anti rabbit), c-Rel (anti-rabbit), ICAM-1 (anti-rabbit), IKKα/β (anti rabbit), IκBα (anti-rabbit), Mdm2 (anti-rabbit), PARP-1/2 (anti-rabbit), Rel-B (anti-rabbit), p50 (anti-rabbit), p53 (anti-mouse), p65 (anti-rabbit) were obtained from Santa Cruz Biotechnology(Santa Cruz, CA, USA).HRP (Horse radish peroxidase)-conjugated secondary antibodies (anti mouse and anti-rabbit) were obtained from Bangalore Genie(Peenya, India). For immuno-fluorescencestudies, secondary antibodiesconjugated toAlexa Fluor (488 and 594, anti-mouse and anti-rabbit) were obtained from Molecular Probes, Invitrogen(Eugene, OR, USA)
    1. immunoprecipitation by using substrate specific antibody or pull-down by affinity trapping the substrate tag. The IP/pull-down complexes wereanalyzed by detecting the ubiquitination of substrate protein by using either substrate specificantibody or ubiquitin antibody through western blotting
    2. using gateway cloning method (Invitrogen). P73domain deletions were cloned in SFB destination vector. WWP2, WWP1, HACE1, E6AP, and PPM1G were cloned into SFB (S-protein/Flag/streptavidin binding protein (SBP) triple tag), GFP,and Myc mammalian destination vectors using the Gateway cloning technology (Invitrogen). WWP2 domain deletions were cloned into Myc-destination vector. WWP1 domain deletions were cloned into SFB-destination vector. PPM1G domain deletions were cloned into SFB mammalian destination vector using Gateway cloning. Bacterially expressing GST-p73, GST-∆Np73, GST-PPM1G, MBP-WWP1, MBP-WWP2, GST-WWP2, GST-WWP1 and GST-HACE1 were generated by using gateway technology. Ubiquitin WT and all the mutants were cloned into hemagglutinin (HA) mammalian destination vector. Flag-tagged Dvl2was purchased from Addgene. Dvl2 domain deletions were cloned into SFB-destination vectors. All the plasmid constructs generated in the present study are mentioned in table 2.Table 2: Plasmids used in the study
    1. ability to take notes anchored to specific passages of text, and have them tagged and searchable across papers, are such major benefits

      For this to happen, start with:

      1. Open an article in the web browser (Google Chrome or Firefox) that can support pdf.js or epub.js or html if that is available
      2. Open hypothesis app, and start annotating at passages (as in this annotation)
      3. Add a consistent tag (see the other how_to tagged articles)
      4. Then search all related hypothesis annotations and notes on pages using those tags and organise knowledge that way.
    1. agree on a standard set of tags to classify sets of resources

      This is step one. Set up a standard set of tags. These tags will then classify resources. Let's say I want to classify all studies on polygenic risk scores on the web. These documents come in the form of HTML, or PDF. If on the top of that, you'd also like to tag something like tutorial, or software to do the job, then write those tags as well when you come across a resource and tag it. So, a list of tags to learn about polygenic risk scores could be something like:

      • polygenic risk score
      • polygenic_risk_score
      • tutorial
      • utility
      • why_how So, all resources that pertain to polygenic risk scores can now be divided into a set of main document types: some that discuss tutorials and how tos, others discuss the utilisation values and debates. Later, these could be reassembled.
  7. Apr 2019
    1. any complaint which asses' milk could possibly relieve

      Donkey milk was considered a viable medical treatment from antiquity (Cleopatra bathed in it) until the turn of the 19th century, when it largely went out of fashion. It was considered a generic cure for a variety of conditions, including gout, scurvy, coughs, colds and asthma. For many, donkey milk caused stomach problems and "lactose intolerance."

      https://georgianera.wordpress.com/tag/asses-milk/

    1. Amdo Group 654 For more information about this term, see Full Entry below. SubjectsLanguage TreeSino-TibetanTibeto-BurmanBodicBodishTibetanFull EntryRelated Subjects (7)Related Texts (12)Related Audio-video (1864)

      KMaps tag: bold, with popover.

    1. 1) We identify and investigate two major kinds of lin-guistic activities on Quora: user level [e.g., basicactivities like posting a question/answer/comment aswell as linguistic styles that involves word/char usage,and part-of-speech (POS) tag usage] and questionlevel [e.g., content, topic associations, and edits for aquestion). Remarkably, many of these activities arefound to have a natural correspondence to the qualitiesthat human judges would consider while deciding if aquestion would remain unanswered (see Table I for a setof motivating examples).2) We perform an extensive measurement study to showthat answerability can be indeed characterized based onthe above-mentioned linguistic activities.3) A central finding is that the language use patterns ofthe users is one of the most effective mechanisms tocharacterize answerability.

    Annotators

    1. appears

      The link is to my blog, but is defective. (a link that works is this, but this is to all occurrences of a tag.) Elsewhere, I found the page that the author was attempting to cite. Here I am not. The text is confused. I have never seen Oliver write "under his brother's name," but he has mostly claimed his brother is different, and then he claimed that he was lying about that and had been lying for years, then he took it back. Liars lie, no way around it. If a liar says "I'm a liar," a sane answer is "Not always." After all, stopped clock.

    2. claim he made this up.

      This is a link to my blog, the URL is broken, though. The core points to this, a tag. Searching that display for "schizo," I find eventually material copied from this page: Authentic Darryl Smith on himself That page is Darryl making the claim of "made up." Recently, socks on Reddit -- probably also Darryl -- have repeated the claim that Oliver denied "schizophrenia," but I have never seen Oliver openly deny it. And it makes sense.

    1. If this is a production situation, and security and stability are important, then just "convenience" is likely not the best deciding factor (any more than leaving your house unlocked all the time might be "convenient").

      如果这是生产情况,安全性和稳定性很重要,那么“便利”可能不是最好的决定因素(不仅仅是让你的房子一直解锁可能是“方便的”)

      • 您可以考虑将每个push to registry的版本 - 以某种形式(毕竟,您发布了新版本的代码,并使其他人可以访问)。
      • :latest与Git存储库中的master分支相当。是否每个push to master都考虑准备投入生产?
      • Releases将(通常)通过验证过程(CI/QA /acceptance/etc)。是否应首先验证master中的更改,并且仅在验证(标记并)部署到生产之后?
      • 发行版(Releases)带有版本;这可以是显式版本(标记),也可以是隐式(不可变标记:图像的摘要)

      显式版本 -- image tag<br> 隐式版本 -- 不可变标记 :image digest

    2. However, there is not a 1:1 relation of digests to tags, so when pulling an image by digest, only the digest is known. If you happen to have an image pulled (manually) with a tag that matches that digest, the tag is shown, but not otherwise

      但是,摘要与标签之间没有1:1的关系,因此在通过摘要pull image时,只知道摘要。如果您碰巧使用与该摘要匹配的标记(手动)拉出图像,则会显示标记,否则不会显示

    1. Impact of the Legion 9 plain 2019-04-16T02:18:41+00:00 Joseph Brown 5eac7ef3705d9f80e567b77e809a27064bd00249 1 2019-04-11T22:00:35+00:00 Joseph Brown 5eac7ef3705d9f80e567b77e809a27064bd00249 Organization of the Legion

      As far as I can tell, you are using the tag function for the purpose of forward and backward movement. I'd reconsider using it for this purpose.

    1. “the expression or application of human creative skill and imagination […] producing works to be appreciated primarily for their beauty or emotional power.”

      Who is the speaker on this quote? Braidotti? I would tag the source or do what you did above (Author last name, page #)

    1. Alexander McQueen: Relationality between human and non-human Charles Sirisawat-Larouche 6 plain 2019-04-10T17:03:44+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 2019-04-01T13:48:01+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 The Deconstructivist Approach Charles Sirisawat-Larouche 22 plain 2019-04-10T16:51:05+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-10T11:43:52+00:00 Alexander McQueen: Relationality between human and non-human 6 plain 2019-04-10T17:03:44+00:00 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 2019-04-10T13:46:55+00:00 The display of its fashion is done toward alien like figure or cyborg incarnation which allow a certain extent of dis-identification. 3 plain 2019-04-10T13:48:27+00:00 1 2019-04-10T12:26:35+00:00 Fashion and Anti-fashion Dichotomy 2 plain 2019-04-10T16:34:01+00:00 1 2019-04-10T12:22:36+00:00 Phallogocentrism 3 plain 2019-04-10T16:38:25+00:00 1 2019-04-10T12:26:06+00:00 Self-Styling and Self-Fashion 3 plain 2019-04-10T16:39:08+00:00 1 media/Fashion-Looks-Forward_Utopian-Bodies_Exhibition_Stockholm-_dezeen_936_12.jpg 2019-04-01T15:44:50+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Post-Gender Charles Sirisawat-Larouche 15 visual_path 2019-04-10T17:26:43+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 2019-04-10T00:30:04+00:00 Rei Kawakubo and the excessive use of material as a strategy to reassert the conceptual embodied entity toward subversive site. 5 Rei Kawakubo and the excessive use of material as a strategy to reassert the conceptual embodied entity toward subversive site. Click and Drag to Annotate plain 2019-04-10T16:17:23+00:00 1 media/IMG_5473 2.jpg media/Animated GIF-original.mp4 2019-04-01T13:55:26+00:00 Thierry Mugler: The contradictory abjection 27 visual_path 2019-04-10T17:32:51+00:00 1 media/2000px-Polka_dots.svg.png media/Animated GIF-original.mp4 2019-04-04T13:16:11+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Posthuman Museum Practices: From Runway to exhibition Charles Sirisawat-Larouche 34 Preceding the essence of fashion tags 2019-04-10T17:47:08+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 2019-04-08T15:25:59+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Self-organizing: Curator, Curatorial. Curating... Charles Sirisawat-Larouche 10 visual_path 2019-04-10T14:09:14+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Contents of this tag: 1 2019-04-04T13:19:50+00:00 Deterritorialize the Fashion Practices 11 vistag 2019-04-10T17:15:57+00:00 1 media/IMG_5473 2.jpg media/Animated GIF-original.mp4 2019-04-01T13:55:26+00:00 Charles Sirisawat-Larouche b6ad6d1a6de4c926cdef3dafe6adb7d5f4702fd8 Thierry Mugler: The contradictory abjection

      Nice idea to use tags. Relevant to the style of assignment.

    1. Can we at this point have the option of also adding a "TAG" function It's much faster to be able to tag as when new questions are being inputted

      Also if the question TAG is shown here, new quizzes can be created on the spot with a specific question TAG (if that makes sense)

    1. Safety advocates urged college students to match the vehicle color and model, the license tag number, and the photo of their ride-share drivers before getting in a vehicle and make the driver say their names to them before they introduce themselves.

      Students should follow this to be safe.

    2. Safety advocates urged college students to match the vehicle color and model, the license tag number, and the photo of their ride-share drivers before getting in a vehicle and make the driver say their names to them before they introduce themselves.

      Always make sure to do this

    1. Dr. Sanha Kim should be moved over to Columbia. We need to add a space holder for Cynthia Loveland under Columbia doctors. For all doctors except Tanya Tag, list them as 'Emergency Veterinarian' instead of emergency and critical care. Under 'doctors' Dr. Tag looks great, could you add Towson & Columbia next to Medical Director? For Dr. Tag under administration, remove her middle initial and change DVM to match DACVECC as displayed for her under doctors. Leave medical director Towson & Columbia. Please display leadership in this order: Angelina Morgan, Tanya Tag, Tamara Santesse, Elizabeth Moyers, Beth Faux, Ashley Carlson, Victoria Syczurowski, Jeff Hawkins, Christine Wolff, Paul Heggie, Joey Sumby (I spelled his name wrong last time)

      Beth Faux should display as "Beth Faux, RVT, VTS (ECC). Her title should be listed as Technician Manager Columbia. Tamara Santesse's title should be Office Manager, Towson. Elizabeth Moyers' title should be Office Manager, Columbia Victoria and Ashley's title are: Technician Co-Manager Towson Jeff Hawkins' title should be Financial Manager Towson & Columbia Paul Heggie's title should be Facilities Manager Towson & Columbia Joey Sumby's title should be Facilities Supervisor Columbia

      Love the super heroes-are there gender specific?

  8. Mar 2019
      • gutes Teaserbild. Allerdings nimmt zuviel Platz. Für den Hauptinhalt (Wanderungen) muss man sofot scrollen. Eine Alternativ wäre mit einem "Karrussell" von den letzten 3 Wanderungsbilder oder so.
    1. Behaviorism

      Learning-Theories.com published a very handy few pages that describe various learning theories. This is a quick, straightforward, simple way to access information on the different theories. This article, Behaviorism, explains that the theory assumes learners learn by responding to external stimuli in their environment. Learning under behaviorism is characterized by a change in the learner's behavior. I use this in my horse training as I use both positive reinforcement (clicker training) and negative reinforcement (pressure-release) to structure my horse's behaviors. Behaviorism can be translated to human work, too. I've used TAG teaching (clicker training for humans) to teach people to get on and off horses with ease and also to trim horses' hooves. I also use it to clicker train my cats! 6/10

    1. Republicans immediately painted the plan as “disastrous,” pointing to studies that suggest the price tag could be as high as $32 trillion.

      Source?<br> Which Republicans?

  9. digitalempathyvet.com digitalempathyvet.com
    1. health exam and vaccinations - $167.53 includes Doctor exam, rabies, Dhlpp, bordetella, send out to lab fecal, and heart worm occult and parish tag. puppy care 1st shots= $137.40 2nd- 9 weeks is $143.29 3rd - 12 weeks $147.01 4th - 15 weeks $187.08 Micro chips- $63.13 feline neuter- $100.89 feline front declaw- $232.87

    1. disallows it in our Public channel and in groups

      You can, however, add a tag to a highlight in order to share it to the Public channel or a group.

    1. personalize learning infographic

      This is not quite what it sounds like. It is a Pinterest style page with links to assorted articles that relate to personalized learning, most of which are presented in an infographic. It is sufficiently useful if one has the patience to click through to the infographics. Usability is satisfactory although the top half of the page is taken up with graphics that are not directly related to the content. rating 3/5

    1. CourseFolk

      I've taken the focus off CourseFolk being educators who craft the lessons and made that something The Lady Alliance does and CourseFolk just does the techy website/members stuff. I think influencers will balk at a random company doing the lesson writing part for them. I'm still happy to do the interviews and workshop the lessons, but maybe I can do that with a Lady Alliance tag somehow. We can chat more about how this will work

  10. Feb 2019
    1. the agent model swaps the in-put and output sequences, and it also takes the tag of filledinformation slots as an input which is extracted from dia-logue in previous turns by pattern matching with the orderinformation in ground truth

      agent model 构建前先预训练。网络结构和user model一样,但是输入和输出反转,同时也把之前对话中已经填充的槽位信息作为输入。但是这俩部分信息并不是简单的直接拼接在一起,而是来学习适合的attention 权重来更好的利用注意力机制。此外任何其他额外的语义意图标签都不必用。

    1. And who will write the software that makes this contraption useful and productive? We will. In fact, we’re already doing it, each of us, every day. When we post and then tag pictures on the community photo album Flickr, we are teaching the Machine to give names to images. The thickening links between caption and picture form a neural net that can learn. Think of the 100 billion times per day humans click on a Web page as a way of teaching the Machine what we think is important. Each time we forge a link between words, we teach it an idea. Wikipedia encourages its citizen authors to link each fact in an article to a reference citation. Over time, a Wikipedia article becomes totally underlined in blue as ideas are cross-referenced. That massive cross-referencing is how brains think and remember.

      This was the main goal of the 'docuverse' where information will be cross referenced and everything will be related in someway to everything else.

    1. tag those and only those who will really derive benefit

      Implies you know who those people are and you may not know but for some of us that is the whole point of an ESN

    1. A study published in October of last year sought to determine how to make best use of digital out-of-home (DOOH) advertisements in the London Underground.7 An example of a DOOH ad would be a digital billboard programed to change the advertisement on display after a specific period of time.  To achieve their goal, the researchers used the same Twitter Streaming API described in the previous study; however, this time they utilized Twitter’s geotagging function (a capability that allows Twitter users to “tag” their location when they post a tweet). Each London Underground station was carefully outlined on a map of London.  Then, the researchers randomly sampled geotagged tweets falling within those zones (meaning the tweeter was at a station).  The specific Underground station, the time of the tweet, and the content of the tweet were all extracted. The researchers continued this practice for one year, seemingly unbeknownst to the Twitter-using patrons of the London Underground, collecting over 10.5 million tweets. This data was then compiled and processed to determine what sort of things people were tweeting about in each London Underground station at certain times of the day on weekdays and on weekends.  For example, nearly 35% of tweets from the Holloway Road station were about sports, and almost 40% of tweets posted between 6 PM and midnight on weekends at the North Greenwich station were about music.8 The authors of the study recommended using this data to create targeted DOOH advertising. For instance, a music-related ad on a rotating digital billboard at night on the weekends in North Greenwich station would probably be more successful than an ad for a sports team.

      This entire passage gives a perfect example of how the data can be broken down and used to make money by advertisers. I intend to allude to the fact that it doesn't stop here.

    1. it allows you to tag records

      Since DH is such a collaborative subject, you have to make sure references are accurate so that everyone gets credit for their contributions

  11. Jan 2019
    1. SGML, HTML, XML

      SGML = Standard Generalized Markup Language, "a standard for how to specify a document markup language or tag set... SGML is not in itself a document language, but a description of how to specify one." So a set of guidelines, like TEI?

    1. With a $5 billion price tag and a $1 billion annual operation cost, the L.H.C. is the most expensive instrument ever built

      That is intense. 5 billion $ to build, yet it cost 1 billion to upkeep.

    1.  .   Social Media is where we will connect with the broader digital storytelling community, which are very active on Twitter and Instagram.  You may participate anonymously if you prefer.  Tag all class related communications with #inte5340 (Links to an external site.)Links to an external site. and #digstory (Links to an external site.)Links to an external site. .

      Would using our personal social media be acceptable for public communication or would you suggest in creating a new account?

    2. Articles and videos will be assigned each week.

      Just wondering what the weekly deadline for discussion will be. Also, do I need to tag these comments...I just saw the option below?

    1. Astros outfielder Josh Reddick is a big wrestling fan, so after he tied the knot this weekend, him and his bride came out to their friends and family using the same entrace as current WWE tag team champions Bobby Roode and Chad Gable.

      "him and his bride came out to their friends"

    1. Machines with interchangeable parts can now be constructed with great economy of effort. In spite of much complexity, they perform reliably. Witness the humble typewriter, or the movie camera, or the automobile. We have reached a point in technology now where all of these inventions can perform their jobs without human involvement.

      His description of the "humble typewriter" seems like such an understatement in comparison to the computers and recording devices we now have

    1. burying their old professional competition in the demand of a common cause, have shared greatly and learned much. It has been exhilarating to work in effective partnership. Now, for many, this appears to be approaching an end.

      What is interesting is how individuals still share their knowledge to create new things, artists and scientists collaborating together, but how there is still that tight restriction that controls what can be done. New inventions, artworks, music, and tools are still copyrighted and individuals must apply to work with them. You also have inventors that place an outrageous price tag on some of their products, limiting the number of people who can buy them and find out what things they can do that are beyond even the mind of the tool's creator.

    1. Hypothesis Annotation instructions:

      1) Make 2-3 annotations. 2) If another student has already annotated a passage you wished to annotate, you have two options: reply to that annotation or choose another passage to annotate. 3) Reply to at least 2 annotations made by your peers 4) If someone replies to your annotation, reply to keep the conversation going. 5) TAG all annotations CITA2019

    1. Proche de la basilique Saint Sernin, haut lieu touristique de la ville rose, le quartier Arnaud Bernard ne profite pas de ce rayonnement. Beaucoup de rues sont sales et le quartier n’attire pas les visiteurs. Des tags et des graffs décorent les murs de certains immeubles. Le quartier fait partie des tout premiers de la ville à accueillir de nombreux artistes graffeurs, mais sans organisation. En 1997 l’association « carrefour culturel » décide de lancer un projet avec les habitants du quartier: réaliser une fresque géante dans la rue Gramat pour couvrir les tags. Après trois ans de discussions avec la municipalité, les propriétaires et les artistes, la fresque peut enfin voir le jour. Les riverains participent eux-mêmes au nettoyage de la rue pour accueillir les dessins. Elle est au cœur d’un projet citoyen. David Brunel explique :  » le but c’est de créer à l’époque des actions culturelles où les citoyens sont à l’initiative, mais aussi acteur du projet du début à la fin » Il poursuit :  » le quartier est vu comme une base d’expérience, avec une réflexion sur le civique ». Parmi les artistes, on retrouve un SDF du quartier, un peintre mais aussi des gens venu d’Espagne. Ils sont tous là pour donner une vision de la ville et de son histoire. Plus qu’une fresque ce sont des échanges, un projet construit par les habitants, pour les habitants. Annick Lodereau, l’une des meneuses, résume le projet à l’époque :  » C’est une fresque anti-tag, une création qui démontre que l’on peut faire des choses ensemble et leur donner un sens ». Malgré l’ampleur du projet, au fil des ans la rue est de nouveau utilisée par les tagueurs qui repassent sur les fresques de l’époque dont il ne reste aujourd’hui plus rien.

      Fais des retours ligne pour marque les paragraphes, sinon c'est trop difficile à lire.

    1. In this section of the paper we broach two aspects of this articulation issue, onefocusing on the management of workflow, the other on the construction and manage-ment of what we term a ‘common information space’. The former concept has beenthe subject of discussion for some time, in the guise of such terms as office automa-tion and more recently, workflow automation. The latter concept has, in our view,been somewhat neglected, despite its critical importance for the accomplishmentof many distributed work activities

      A quick scan of ACM library papers that tag "articulation work" seems to indicate the "common information space" problem still has not attracted a lot of study. This could be a good entry point for my work with CSCW because time cuts across both workflow and information space.

      Nicely bundles boundary infrastructure, sense-making and distributed work

  12. Dec 2018
  13. spilledreality.tumblr.com spilledreality.tumblr.com
    1. But the real sex politics are more ingrained and foundational, relating to how ‘Tag perceives herself in the world and how that self-image as object lends itself to a specific and perhaps primarily female mode of suffering.

      A. Saramandi: "She’s victim of a culture that bombards her with the message that she is in control, as long as she buys the necessary products, looks a certain way. In one of the most poignant moments of the novel, she doesn’t realise that this agency is a false god: when the narrator is molested by one of her father’s colleagues, she believes she was “letting [him] kiss me” and is confused as to why, since after all, as the epitome of desire, she must be responsible."

    1. The real question, I realized, was: how can I remember anything if I can only keep it in one place?

      The biggest issue I have with iThoughts right now is the lack of ability to dynamically filter maps based on tags or other properties of nodes. I'd love to be able to view only the nodes I need for a specific query and have everything else dynamically vanish— particularly on large maps, where the existing method of filtering renders the nodes illegible...

      That said, I've come to depend on text searches to return a filtered list of nodes. iThoughts allows for searching within a map and across all maps in the filesystem. It's not a fuzzy search, so search strings have to be constructed in a fixed way which necessitates a robust system of memorable tags, categories or conventions (for example, use of the tag "@due(2019-10-02)" allows me to filter for nodes with due dates, and nodes due within a specific year, month or on a specific date, by parsing incremental fragments of the tag. Simple, but effective.

    1. Tag Cloud

      Le nuage de mots surcharge la page

      Un nuage de mots n'apporte pas beaucoup d'informations aux utilisateur·rice·s qui arrivent sur cette page.

      En revanche, cela surcharge la page à la place d'autres informations qui pourraient être plus utiles.

      Suggestion : placer ce nuage de mots sur une page spécifique que les utilisateurs que ce nuage de mots intéresse pourront consulter.

    1. We therefore developed protocols for fluorescent in situ hybridization (FISH) targeting specific ascomycete and cystobasidiomycete rRNA sequences.

      A molecular probe is created against a specific DNA sequence, in this case rRNA from either ascomycetes or basidiomycetes. The probe is designed with a recognizable tag on the end. Then a second probe is used to match the first probe, and finally a third probe to match the second one with a fluorescent tag on it. By using multiple probes we can amplify even the smallest signals to our first probe. These probes are then mixed with a biological sample that is fixed in place. If fluorescence is observed the probe has found a target.

      In some experiments, as in this example, researchers use multiple probes in a single experiment. This provides information about how close two organisms of interest are in a given sample.

    1. Pong in 1972 and Space Invaders in 1978, which kick started a cultural revolution. Notably in 1981, with Nintendo’s masterpiece, Donkey Kong

      These were the first games to really kickstart video games' jump into popular culture. More than that, they left their mark on society as the first games to be accessible in your homes, which is an important note. That was the start of gaming being right in people's faces, as they have become everywhere now.

    1. seen romantically situated among wood on a high eminence at some little distance

      This description of a cottage reminds me of the contrast in Austen's Sense and Sensibility between how the upper classes and the landed gentry view cottages. The upper classes view cottages in a romantic way as cute, comfy homes, however the landed gentry know that cottages result out of a neccesity brough on by an oppresive and restrictive economic system.

      https://janeaustensworld.wordpress.com/tag/19th-century-cottages/

    2. leeches

      "Bloodletting procedures, including leeching, became the most common medical procedure throughout the early modern period. By the early 19th century, many patients regularly submitted to various bloodletting practices as a means of preventing or treating infection and disease"

      https://www.britannica.com/science/leeching

      Leeching more specifically in Jane Austen's world: "One can imagine that during her final illness, Jane Austen was no stranger to leeches."

      https://janeaustensworld.wordpress.com/tag/leeches/

    1. KnowFlow in a visual knowledge management enviroment for collaborative groups. Collaborative SAAS, purely web-based multi-user application.

      Although it is been created as a part of Neuroweb platform, it seems, that roots behind Douglas Engelbart conceptual framework and Neuroweb conceptual framework are the same. As one can see when reading D.Engelbart's group documents, cognitive science concepts were used in a context very similar to what is considered to be a conceptual framework of Cultural-historical psychology approach, based on undestanding of concept developement and role of symbolic representations in development of psyche and culture. One can see citations of authors connected to cultural-historical psychology throughout the works of Engelbart's group, i.e. of Alan's Keys and other members. KnowFlow system as it is and in scope of it's developement is a system that can easily be understood via DKR conceptual approach. It does not mean that it was build on D.Engelbarts framework alone, but still all the basic concepts of DRK are there. We build KnowFlow to be a living knowledge network, providing means to import, create, modify and view and export knowledge in a process of any complex product or project developement by a group of knowledge workers. That is basically a tool to boost group's IQ, its collective exocortex. KnowFlow can import different kinds of documents, including texts, maindmaps, presentations, media materials. We can do it via direct import, import from connected sources via public API, or via WebClipper that works as a Chrome Browser extention similar to one of Evernote, that can grab a page, fragment of text or a screenshot to save it as a knowledge element, rich format note. If the initial imported document is too large or consists of many subdocuments, special import algorythm can parce it up to a set of knowledge elements, saving each of them separately. Semantic engine is used to help user to parce the documents, finding the key statements in text, separating them and even instantly creating a concept-map our of the text.

      User can work individually or with a group of other users on this knowledge material in different ways: He can work with element as a text, using simple text editor, adding additional materials to a body of the knowledge element, making a group of elements form one element or combining several elements into a group that can be presented as a group or as a complex element at the same time. One can also use element (card) templates to make his work easier. User can also work with his collection of knowledge as with a database, searching through elements, filtering them, group or combine them in a "collection view". One can use domain system to tag or classify elements of knowledge he is working with. Domain is a set of classes that can also me named ontology. So, user can place elements in context of different ontologies. User can work with elements or groups of elements (that can be seen as fractal elements) in a concept-map view. It means that one can connect elements with cinnection, set relations between them and work with them as one works with knowledge maps. One card (knowledge element) can be used in different maps, still being the same element, changing in all the maps it is being used it. Or elements can be copied or modified, That way the system will know who and how used elements to build a new knowledge upon them. Version control is also there. One can use his collection view not for his whole collection but for one knowledge space (map). This view disregards connections, still providing means to work with groups of cards and stacks or sets, similar to Kanban decs used in Trello (and yes we have an integration with trello. too) Knowledge spaces (maps) can be connected to each other through "portals" - these are cards (elements) or groups of cards that are present in several maps (knowlege spaces) at the same time. This way knowledge workers can create a multi-space flow of knowledge between different maps, belonging to one or many groups, working with different aspects of knowledge and different stages of its life-cycle. A special instrument - "Сass" can be used to import knowledge elements or portals from other maps during group work. Users can instantly view the map (knowledge space) in presentation view, when each element is one slide. navigating through complex knowledge structure, choosing different trajectories depending on what they want to focus on. Additional collaboration tools include, but not limited to voting, commentaries end collective editing of knowledge in any view, as well as using shared knowledge space templates for different kinds of collaboration (brainstorms, SWOT analisys etc), collaborators can share elements of knowledge, collections, maps or it branches between individual users, groups or make them publicly available.<br> All that work is augmented with voice recognition that lets users speak their knowledge in - and that is particularly useful in capruring debates or meetings. All functions of the KnowFlow system are augmented with machine learning semantic recommendation engine. That recommends fragments of knowledge already in the system, recommends templates and connections in knowledge map mode. We also have a Virtual Relity interface that can give one an opportunity to view multiple knowledge spaces at the same time, walk between knowledge networks and see them being created in real time.

      All the results can be exported in any moment to a number of formats (including PDF, Mindmanager, Table, Text, etc) or streamed through API to other elements for additional ways of use. One of uses of KnowFlow is creation a templates or publically available knowledge spaces, knowledge elements, maps or chains that contain best practices and knowledge that can be used in other contexts, when needed.

      Next version will also include timeline views usefull for sorting all the knowledge elements on a timeline. filtered by meta-data, VR interface will get edit capabilities and voice commands will be available for a group work in shared spaces. Additional libraries of semanic analisis will be added, text edit mode will be upgraged, and number of other software integrations will provide easy use of KnowFlow as a collaborative knowledge processing tool in any workflows.

      That is, basically, DKR, as it seems to me.

      “A dynamic knowledge repository is a living, breathing, rapidly evolving repository of all the stuff accumulating moment to moment throughout the life of a project or pursuit. This would include successive drafts and commentary leading up to more polished versions of a given document, brainstorming and conceptual design notes, design rationale, work lists, contact info, all the email and meeting notes, research intelligence collected and commented on, emerging issues, timelines, etc.” That are the forms of data that can be inmorted, used, transformed and contextually accessed, History tool provides ability to move back in time to a point in knowledge space developement, to access relevant data and fork new line of work from there, still being able to use all the knowledge being created later or in lateral lines of work, even by other groups, proven that access mode for them makes it available.

      One can say that knowflow is created as a version of CoDIAK process, as it supports and speeds up ways to develop, integrate, and apply all this iterating knowledge from the swirl of disparate concurrent contributions. So, if KnowFlow is an “emerging collective record of all this activity captured on the fly -- the emerging collective vision, know-how, the group brain, memory, where the dots are connected and the right hand knows what the left hand is doing” it means that KnowFlow is really a version of DKR .

      KnowFlow emerged as a result of best practices of work with project groups in business schools, methods and approaches to collective work (i.e. Rapid Foresight methodology, co-developed by KnowFlow team members, etc) and is already used to facilitiate the collective work “in a way that optimizes the capture, organization, and enhanced utility of the DKR”. Best practices would include special roles such as collaboration facilitators, as well as high performance knowledge specialists, who are continuously moderating the connectivity, capture, tagging, tracking, and portals into the emerging repository -- tools, practices, and people connecting in a way that renders the DKR optimally navigable, searchable, and useful.

  14. Nov 2018
    1. only a small fraction of the neurons that receive odor information respond to each odor

      Lin et al. discovered the neurological process that controls why so few olfactory neurons make up each odor "tag."

      The reason is because when the cells that receive odor information, called Kenyon cells (KCs), become active, the anterior paired lateral neuron responds by slowing the activity of the KCs. The more active the KCs become, the harder the anterior paired lateral neuron works to block their activity. The few KCs that are left active after this process form the odor tag.

    1. We have lost any sense of what we mean when we call a university “public.

      It really loses the tag of public when it's hundred of dollars to take a single class almost anywhere.

    1. Partial charges and discharges that combine to 100% are counted as a single full cycle

      Really? I don't think anyone has tested partial charges and discharges. I would like to see a reference for this point!

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  15. Oct 2018
    1. Oates soon realized navigating Wikimedia Commons and finding interesting materials is challenging. She noted how the category system used to organize and tag media files on Commons is confusing and hides—not shows—the richness of content there.

      Problems with the category system making it difficult to search relevant information

    1. agree, but "you can't prove a negative".

      It's easy to mess up the quotation formatting. Also easy to fix with a close-quote tag. That errors like this commonly persist shows that people don't go back and read their own writing.

    1. Margaretta M. Lovell #pt-cv-view-29709a7js0.pt-cv-post-border { margin: 0; border-top-width: 1px; border-left-width: 1px } #pt-cv-view-29709a7js0.pt-cv-post-border { margin: 0; border-top-style: solid; border-left-style: solid } #pt-cv-view-29709a7js0.pt-cv-post-border .pt-cv-content-item { border-right-width: 1px; border-bottom-width: 1px; border-right-style: solid; border-bottom-style: solid; } #pt-cv-view-29709a7js0 .pt-cv-title a, #pt-cv-view-29709a7js0 .panel-title { font-weight: 600 !important; } #pt-cv-view-29709a7js0 .pt-cv-hover-wrapper::before { background-color: rgba(0,0,0,.3) !important; } #pt-cv-view-29709a7js0 .pt-cv-content-item:hover .pt-cv-hover-wrapper::before { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-29709a7js0:not(.pt-cv-nohover) .pt-cv-mask * { color: #fff; } #pt-cv-view-29709a7js0 .pt-cv-carousel-caption { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-29709a7js0 .pt-cv-specialp * { color: #fff !important; background-color: #CC3333 !important; } #pt-cv-view-29709a7js0 .pt-cv-pficon { color: #bbb !important; } #pt-cv-view-29709a7js0 .add_to_cart_button, #pt-cv-view-29709a7js0 .add_to_cart_button * { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-29709a7js0 .woocommerce-onsale { color: #ffffff !important; background-color: #ff5a5f !important; } #pt-cv-view-29709a7js0 .pt-cv-readmore { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-29709a7js0 .pt-cv-readmore:hover { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-29709a7js0 + .pt-cv-pagination-wrapper .pt-cv-more , #pt-cv-view-29709a7js0 + .pt-cv-pagination-wrapper .pagination .active a { color: #ffffff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-29709a7js0'] .active.pt-cv-filter-option, [id^='pt-cv-filter-bar-29709a7js0'] .active .pt-cv-filter-option, [id^='pt-cv-filter-bar-29709a7js0'] .selected.pt-cv-filter-option, [id^='pt-cv-filter-bar-29709a7js0'] .dropdown-toggle { color: #fff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-29709a7js0'] .pt-cv-filter-title { color: #fff !important; background-color: #00aeef !important; } #pt-cv-gls-29709a7js0 li a.pt-active { color: #fff !important; background-color: #ff5a5f !important; } #pt-cv-view-29709a7js0 .pt-cv-gls-header { color: #fff !important; background-color: #00aeef !important; } #pt-cv-view-29709a7js0 .cvp-responsive-image[style*="background-image"] { width: 200px; height: 200px; } #pt-cv-view-29709a7js0 .pt-cv-ocol:nth-child(2n+2) .cvp-responsive-image[style*="background-image"] { width: 150px; height: 150px; } Bully PulpitWhat Is the Role of Patriotism in the Study of American Art?M. Elizabeth Boone, Lauren LessingM. Elizabeth (Betsy) Boone and Lauren Lessing, Executive Editors Do we have a responsibility to the nation in our teaching and writing on American art? How would this responsibility be enacted and can it be done without seeming to be jingoistic? Does a new understanding of our nation impact our teaching and study of American art? How do you feel about the idea of a national narrative? Have you been challenged to rethink the relationship between American art, the nation, and its citizenry at any time in the last year?ResponsesDavid M. Lubin, Charlotte C. Weber Professor of Art, Wake Forest UniversityAngela Miller, Professor, Department of Art History and Archaeology, Washington University, Saint LouisPatricia Junker, Ann M. Barwick Curator of American Art, Seattle Art MuseumLauren Lessing, Mirken Director of Academic and Public ProgramsAlan Wallach, Professor Emeritus, Department of Art and Art History, The College of William and MarySally Webster, Professor Emerita, City University of New YorkResearch Notes “Kicked About”: Native Culture at Thomas Jefferson’s MonticelloKristine K. RonanKristine K. Ronan, Independent Scholar Two of the most prominent Native-made objects in Jefferson’s original hall were a pair of male and female figures that Jefferson had received several years prior to Lewis and Clark’s shipments. Curiously, the figures had disappeared from the historical record with Jefferson’s death in 1826. It came as quite a surprise, then, that during my #pt-cv-view-6f0799d3kr.pt-cv-post-border { margin: 0; border-top-width: 1px; border-left-width: 1px } #pt-cv-view-6f0799d3kr.pt-cv-post-border { margin: 0; border-top-style: solid; border-left-style: solid } #pt-cv-view-6f0799d3kr.pt-cv-post-border { margin: 0; border-top-color: #343434; border-left-color: #343434 } #pt-cv-view-6f0799d3kr.pt-cv-post-border .pt-cv-content-item { border-right-width: 1px; border-bottom-width: 1px; border-right-style: solid; border-bottom-style: solid; border-right-color: #343434; border-bottom-color: #343434; } #pt-cv-view-6f0799d3kr .pt-cv-title a, #pt-cv-view-6f0799d3kr .panel-title { font-weight: 600 !important; } #pt-cv-view-6f0799d3kr .pt-cv-title a:hover, #pt-cv-view-6f0799d3kr .panel-title:hover { color: #b53328 !important; } #pt-cv-view-6f0799d3kr .pt-cv-hover-wrapper::before { background-color: rgba(0,0,0,.3) !important; } #pt-cv-view-6f0799d3kr .pt-cv-content-item:hover .pt-cv-hover-wrapper::before { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-6f0799d3kr:not(.pt-cv-nohover) .pt-cv-mask * { color: #fff; } #pt-cv-view-6f0799d3kr .pt-cv-carousel-caption { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-6f0799d3kr .pt-cv-specialp * { color: #fff !important; background-color: #CC3333 !important; } #pt-cv-view-6f0799d3kr .pt-cv-pficon { color: #bbb !important; } #pt-cv-view-6f0799d3kr .add_to_cart_button, #pt-cv-view-6f0799d3kr .add_to_cart_button * { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-6f0799d3kr .woocommerce-onsale { color: #ffffff !important; background-color: #ff5a5f !important; } #pt-cv-view-6f0799d3kr .pt-cv-readmore { color: #ffffff !important; background-color: #2a6ca0 !important; } #pt-cv-view-6f0799d3kr .pt-cv-readmore:hover { color: #ffffff !important; background-color: #000000 !important; } #pt-cv-view-6f0799d3kr + .pt-cv-pagination-wrapper .pt-cv-more , #pt-cv-view-6f0799d3kr + .pt-cv-pagination-wrapper .pagination .active a { color: #ffffff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-6f0799d3kr'] .active.pt-cv-filter-option, [id^='pt-cv-filter-bar-6f0799d3kr'] .active .pt-cv-filter-option, [id^='pt-cv-filter-bar-6f0799d3kr'] .selected.pt-cv-filter-option, [id^='pt-cv-filter-bar-6f0799d3kr'] .dropdown-toggle { color: #fff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-6f0799d3kr'] .pt-cv-filter-title { color: #fff !important; background-color: #00aeef !important; } #pt-cv-gls-6f0799d3kr li a.pt-active { color: #fff !important; background-color: #ff5a5f !important; } #pt-cv-view-6f0799d3kr .pt-cv-gls-header { color: #fff !important; background-color: #00aeef !important; } #pt-cv-view-6f0799d3kr .cvp-responsive-image[style*="background-image"] { width: 150px; height: 150px; } Book ReviewsConsuming Stories: Kara Walker and the Imagining of American Race Enduring Truths: Sojourner’s Shadows and Substance The American School: Artists and Status in the Late Colonial and Early National Era Building the British Atlantic World: Spaces, Places, and Material Culture, 1600–1850 The Early American Daguerreotype: Cross-Currents in Art and Technology #pt-cv-view-4b4916esnz.pt-cv-post-border { margin: 0; border-top-width: 1px; border-left-width: 1px } #pt-cv-view-4b4916esnz.pt-cv-post-border { margin: 0; border-top-style: solid; border-left-style: solid } #pt-cv-view-4b4916esnz.pt-cv-post-border { margin: 0; border-top-color: #343434; border-left-color: #343434 } #pt-cv-view-4b4916esnz.pt-cv-post-border .pt-cv-content-item { border-right-width: 1px; border-bottom-width: 1px; border-right-style: solid; border-bottom-style: solid; border-right-color: #343434; border-bottom-color: #343434; } #pt-cv-view-4b4916esnz .pt-cv-title a, #pt-cv-view-4b4916esnz .panel-title { font-weight: 600 !important; } #pt-cv-view-4b4916esnz .pt-cv-title a:hover, #pt-cv-view-4b4916esnz .panel-title:hover { color: #b53328 !important; } #pt-cv-view-4b4916esnz .pt-cv-hover-wrapper::before { background-color: rgba(0,0,0,.3) !important; } #pt-cv-view-4b4916esnz .pt-cv-content-item:hover .pt-cv-hover-wrapper::before { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-4b4916esnz:not(.pt-cv-nohover) .pt-cv-mask * { color: #fff; } #pt-cv-view-4b4916esnz .pt-cv-carousel-caption { background-color: rgba(51,51,51,.6) !important; } #pt-cv-view-4b4916esnz .pt-cv-specialp * { color: #fff !important; background-color: #CC3333 !important; } #pt-cv-view-4b4916esnz .pt-cv-pficon { color: #bbb !important; } #pt-cv-view-4b4916esnz .add_to_cart_button, #pt-cv-view-4b4916esnz .add_to_cart_button * { color: #ffffff !important; background-color: #00aeef !important; } #pt-cv-view-4b4916esnz .woocommerce-onsale { color: #ffffff !important; background-color: #ff5a5f !important; } #pt-cv-view-4b4916esnz .pt-cv-readmore { color: #ffffff !important; background-color: #2a6ca0 !important; } #pt-cv-view-4b4916esnz .pt-cv-readmore:hover { color: #ffffff !important; background-color: #000000 !important; } #pt-cv-view-4b4916esnz + .pt-cv-pagination-wrapper .pt-cv-more , #pt-cv-view-4b4916esnz + .pt-cv-pagination-wrapper .pagination .active a { color: #ffffff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-4b4916esnz'] .active.pt-cv-filter-option, [id^='pt-cv-filter-bar-4b4916esnz'] .active .pt-cv-filter-option, [id^='pt-cv-filter-bar-4b4916esnz'] .selected.pt-cv-filter-option, [id^='pt-cv-filter-bar-4b4916esnz'] .dropdown-toggle { color: #fff !important; background-color: #00aeef !important; } [id^='pt-cv-filter-bar-4b4916esnz'] .pt-cv-filter-title { color: #fff !important; background-color: #00aeef !important; } #pt-cv-gls-4b4916esnz li a.pt-active { color: #fff !important; background-color: #ff5a5f !important; } #pt-cv-view-4b4916esnz .pt-cv-gls-header { color: #fff !important; background-color: #00aeef !important; } #pt-cv-view-4b4916esnz .cvp-responsive-image[style*="background-image"] { width: 150px; height: 150px; } Exhibition ReviewsSoul of a Nation: Art in the Age of Black Power The Wall of Respect: Vestiges, Shards and the Legacy of Black Power / Eugene Eda’s Doors for Malcolm X College We Wanted a Revolution: Black Radical Women, 1965–85 Marsden Hartley’s Maine #pt-cv-view-66aa710wr8.pt-cv-post-border { margin: 0; border-top-width: 1px; border-left-width: 1px } #pt-cv-view-66aa710wr8.pt-cv-post-border { margin: 0; border-top-style: solid; border-left-style: solid } #pt-cv-view-66aa710wr8.pt-cv-post-border { margin: 0; border-top-color: #343434; 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} #pt-cv-gls-66aa710wr8 li a.pt-active { color: #fff !important; background-color: #ff5a5f !important; } #pt-cv-view-66aa710wr8 .pt-cv-gls-header { color: #fff !important; background-color: #00aeef !important; } #pt-cv-view-66aa710wr8 .cvp-responsive-image[style*="background-image"] { width: 150px; height: 150px; } Home | Contact Publishing Services | My Account Privacy | Acceptable Use of IT Resources The copyright of these individual works published by the University of Minnesota Libraries Publishing remains with the original creator or editorial team. For uses beyond those covered by law or the Creative Commons license, permission to reuse should be sought directly from the copyright owner listed in the About pages.

      This tag should take the reader to The Gustatory Turn as well as the two essays that are being referenced.

  16. allred720fa18.commons.gc.cuny.edu allred720fa18.commons.gc.cuny.edu
    1. the consequent prolonged beating about, the past sufferings from obstinate calms, and still continued suffering from thirst; in all these points, as well as others, Don Benito’s story had corroborated not only the wailing ejaculations of the indiscriminate multitude, white and black, but likewise–what seemed impossible to be counterfeit–by the very expression and play of every human feature, which Captain Delano saw.

      These "obstinate calms" probably refer to the Intertropical Convergence Zone, or ITCZ. Also know as "the doldrums," in this zone around the equator wind currents of the northern and southern hemisphereshttps://en.wikipedia.org/wiki/Doldrums converge. The doldrums are known for both storms and minimal wind. "Colloquially, the "doldrums" are a state of inactivity, mild depression, listlessness, or stagnation" - which also seems to be the prevailing mood of Benito Cereno, as Capt. Delano perceives him. See also: Page note 1, Tag: "doldrums"

    1. The role of touch in the multi-sensory experience of reading turns out to be as important as we intuit it to be when we hold a volume or turn a page — or better yet, when we mark it up.

      I've found that the way I read and my reading retention have changed since I started to regularly use digital annotation. The act of selecting what sentence to highlight, how to tag passages and articles, and what to make public has changed how I feel about reading online. I still prefer paper for pleasure reading, but for news, research, and collaborative reading, digital now works just fine for me.