First having read the book of myths, and loaded the camera, and checked the edge of the knife-blade,
this represents the conscious effort to go beyond the surface stories 'myths' and confront the deeper, often hidden realities 'the wreck'
173 Matching Annotations
- Oct 2025
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poets.org poets.org
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There is a ladder. The ladder is always there hanging innocently close to the side of the schooner. We know what it is for, we who have used it.
I was so suprised that the author uses ladder to represnts the fear of his alone.
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whose drowned face sleeps with open eyes whose breasts still bear the stress
I love the author writes about the stress that the author can't support about it.
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my mask is powerful
What is the mask is about? Is a phase or a another thing?
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I stroke the beam of my lamp slowly along the flank of something more permanent than fish or weed
As the author stroke his beam of his lamp on the flank, what he was doing can be looking back the wounds and emotions he had. And it's something that comes and never goes, unlike fish or weed, which doesn't leave lasting impact to our brain.
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This is the place. And I am here, the mermaid whose dark hair streams black, the merman in his armored body.
The speaker finally reached the wreck and become part of it. Calling herself both the mermaid and the merman could suggest that she feels connected to different sides of human experience, not limited by gender.
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I came to explore the wreck. The words are purposes. The words are maps.
This part might mean that the speaker’s goal is to truly explore and understand the wreck, not just read about it. The “words” could represent stories or explanations that guide her, like maps, but she still needs to see the truth for herself.
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I go down. My flippers cripple me, I crawl like an insect down the ladder and there is no one to tell me when the ocean will begin.
This might describe how the she feels awkward and alone while going deeper into the sea. The image of crawling “like an insect” could suggest weakness or discomfort.
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First having read the book of myths, and loaded the camera,
I see nothing in common at this point, and cannot associate the two lines.
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I put on the body-armor of black rubber the absurd flippers the grave and awkward mask.
I think it’s interesting how the ordinary diving equipment becomes symbolic, like “black rubber body armor” and “awkward mask,” suggesting emotional or psychological protection.
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And now: it is easy to forget what I came for among so many who have always lived here
I think when she says it's easy to forget what she came for, it;s because there's so much to take in down there ; not just the wounds and broken things, but also strange , hauntingly beautiful details that make you want to stay and wander.Tat's why there seem to be people who have 'always lived here' - they've settles among the ruins, maybe trapped by it or maybe finding a kind of comfort in it.
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We are, I am, you are by cowardice or courage the one who find our way back to this scene carrying a knife, a camera a book of myths in which our names do not appear.
I think this is powerful because it shows that we are all part of this search. Even if our names weren't written in the old stories, we can still go down there and see the wreck for ourselves. I think the wreck here might be our traumas or things we try to forgetting. this line is really deep
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the drowned face always staring toward the sun the evidence of damage worn by salt and sway into this threadbare beauty the ribs of the disaster curving their assertion among the tentative haunters.
I love how she describes the wreck so vividly that we can picture it. it makes me think of the dark times in our own lives when we wanted to reach the sunlight so badly. some things we believed would last forever ended up drifting into the 'ocean of lost memories' I especially like the part about the ribs, it feels like those old hurts we try to ignore, but every now and then they poke us again. we can't really erase them ;we just learn to move around them, like tentative haunters of our own past.
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There is a ladder. The ladder is always there hanging innocently close to the side of the schooner. We know what it is for, we who have used it
the ladder is a symbol of a way into depths of history/memory, but here she wrote 'we who have used it' implying that not everybody chooses descend..Only the ones who choose to climb down can really start to face themselves and hidden truths.
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I am having to do this not like Cousteau with his assiduous team aboard the sun-flooded schooner but here alone.
unlike heroic male explorers, the speaker dives alone leads to feminist emphasis on personal, solitary quest
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poets.org poets.org
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Two roads diverged in a yellow wood,And sorry I could not travel both
I think the two roads symbolize the choice we all face in life - one may be destiny and the other fate, but no matter which we choose , it leads us to a certain way of living and becoming ourselves.
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drive.google.com drive.google.com
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Whatever she planted grew as if by magic, and her fame as a grower of .flowers spread over three counties. Because of her creativity with her flow-ers, even my memories of poverty are seen through a screen of blooms-sun-flowers, petunias, roses, dahlias, forsythia, spirea, delphiniums, verbena ...and on and on.
Her mother's garden symbolizes suppressed female creativity-beauty and art growing from struggle, like magic.
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For it needs little skill and psychology to be sure that a highlygifted girl who had tried to use her gift for poetry would have been sothwarted and hindered by contrary instincts [add "chains, guns, the lash, theownership of one's body by someone else, submission to an alien religion"Lthat she must have lost her health and sanity to a certainty."
Society once saw creative women as "mad", but their so-called madness is actually passion, vision and art.
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- Sep 2025
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www.sefaria.org www.sefaria.org
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https://www.sefaria.org/topics/ben-sorer-umoreh?sort=Relevance&tab=notable-sources The Ben Sorer Umoreh — known in English as the“wayward and rebellious son”
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- Jun 2025
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www.reddit.com www.reddit.com
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Not unusual to see regular pica spaced rulers on very small (18-21 cpi). They weren't common enough to warrant making new rulers.
via u/Koponewt (Otto)
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- May 2025
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www.theguardian.com www.theguardian.com
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Eine Studie aus dem Jahr 2021 zeigt, dass Männer in Schweden 16% mehr klimaschädliche Emissionen verursachen als Frauen, obwohl sie ähnliche Geldbeträge ausgeben. Der Hauptgrund ist der höhere Kraftstoffverbrauch für Autos. Die Studie, veröffentlicht im Journal for Industrial Ecology, ergab, dass über die Hälfte der Emissionen durch Nahrung und Urlaube verursacht werden. Eine pflanzenbasierte Ernährung und Zugreisen könnten die Emissionen um 40% senken. Die EU wurde kritisiert, weil ihr Green Deal geschlechtsspezifische Unterschiede nicht berücksichtigt. [Zusammenfassung mit Mistral generiert] https://www.theguardian.com/environment/2021/jul/21/men-cause-more-climate-emissions-than-women-study-finds
Tags
- Annika Carlsson Kanyama
- climate emergency
- meat consumption
- European Environmental Bureau
- by: Damian Carrington
- consumption habits
- travel
- Shifting expenditure on food, holidays, and furnishings could lower greenhouse gas emissions by almost 40%
- ecofeminism
- gender
- 2021-07-21
- Nadège Lharaig
Annotators
URL
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- Mar 2025
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www.theguardian.com www.theguardian.com
Tags
- ACLU’s Speech, Privacy, and Technology Project,
- 2025-03-21
- event: ruling against Greenpeace in North Dakota 2025-03-19
- Standing Rock Grassroots
- Michael Gerrard
- Slapp suits
- International Center for Not-for-Profit Law
- by: Rachel Leingang
- Amnesty International
- USA
- Waniya Locke
- Dakota access pipeline
- Brian Hauss
- Sushma Raman
- Sabin Center for Climate Change Law at Columbia Law School
- Energy Transfer
- Greenpeace
Annotators
URL
- Feb 2025
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www.derstandard.at www.derstandard.at
- Dec 2024
- Nov 2024
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www.theguardian.com www.theguardian.com
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usrussiarelations.org usrussiarelations.org
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Mikhail Lomonosov
Tell us more! Who was Mikhail Lomonosov, and what was he doing during this time period?
Cite your source(s)!
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medium.com medium.com
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for - article - Medium - Translating vision into sound - A deep learning perspectiive - Viktor Toth - 21 April, 2019 - from - search - Google - android app "The Voice" translates images into audio signal - https://hyp.is/OJKKmJ1MEe-TAp_w_0SK_Q/www.google.com/search?q=android+app+%22The+Voice%22+translates+images+into+audio+signal&sca_esv=6fa4053b1bfce2fa&sxsrf=ADLYWIK_UqZZZ9OCRCwH4D6FoSaykbMTpQ:1731013461104&ei=VSstZ4eCBqi8xc8P5KP_kAU&ved=0ahUKEwjHgM3Tj8uJAxUoXvEDHeTRH1IQ4dUDCA8&uact=5&oq=android+app+%22The+Voice%22+translates+images+into+audio+signal&gs_lp=Egxnd3Mtd2l6LXNlcnAiO2FuZHJvaWQgYXBwICJUaGUgVm9pY2UiIHRyYW5zbGF0ZXMgaW1hZ2VzIGludG8gYXVkaW8gc2lnbmFsMggQABiABBiiBDIIEAAYgAQYogQyCBAAGIAEGKIEMggQABiABBiiBDIIEAAYgAQYogRI2xdQpglYjRJwAXgCkAEAmAGZA6ABmQOqAQM0LTG4AQPIAQD4AQGYAgOgAqADwgIKEAAYsAMY1gQYR8ICBBAAGEeYAwDiAwUSATEgQIgGAZAGCJIHBTIuNC0xoAewBA&sclient=gws-wiz-serp
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- Oct 2024
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www.youtube.com www.youtube.com
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1:21:54 If a community moved to a WELLNESS model rather than an ILLNESS model, it would generate millions of dollars in saved resources 1:21:54 If a community moved to a PREVENTION model rather than a CURE Model, it would generate millions of dollars in resources
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1:21:44 By identifying WASTE - we can identify capacity to create VALUE for ALL
Tags
- 1:21:54 If a community moved to a WELLNESS model rather than an ILLNESS model, it would generate millions of dollars in saved resources 1:21:54 If a community moved to a PREVENTION model rather than a CURE Model, it would generate millions of dollars in resources
- 1:21:44 By identifying WASTE - we can identify capacity to create VALUE for ALL
Annotators
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- Sep 2024
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www.pewresearch.org www.pewresearch.org
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23% of news webpages contain at least one broken link, as do 21% of webpages from government sites.
for - stats - digital decay - 23% of news webpages contain at least one broken link, - stats - digital decay - 21% of webpages from government sites contain at least one broken link
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aacrjournals.org aacrjournals.org
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RRID: CVCL_4784
DOI: 10.1158/0008-5472.CAN-21-3822
Resource: (RRID:CVCL_4784)
Curator: @dhovakimyan1
SciCrunch record: RRID:CVCL_4784
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aacrjournals.org aacrjournals.org
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RRID:Addgene_58779
DOI: 10.1158/0008-5472.CAN-21-2072
Resource: RRID:Addgene_58779
Curator: @dhovakimyan1
SciCrunch record: RRID:Addgene_58779
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- Aug 2024
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www.literatureandlatte.com www.literatureandlatte.com
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www.jerrymichalski.com www.jerrymichalski.comHOME1
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https://www.jerrymichalski.com/
2024-08-21: JM indicates that he's revamped his personal website.
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en.wikipedia.org en.wikipedia.org
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https://en.wikipedia.org/wiki/Mixedink
MixedInk was a startup that provided web-based, collaborative writing software enabling large groups of people to create text that expresses a collective opinion, such as a mission statement, editorial, political platform, open letter or product review.
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www.midjourney.com www.midjourney.com
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https://www.midjourney.com/home
2024-08-21: PM indicates they're doing free trials now
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github.com github.com
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Take ownership of your Twitter data and get your tweets back
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pinokio.computer pinokio.computerPinokio1
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https://pinokio.computer/<br /> Pinokio is a browser that lets you install, run, and programmatically control ANY application, automatically.
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https://flux1.ai/<br /> Flux AI Image Generator
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glif.app glif.app
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https://glif.app/glifs
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- Jul 2024
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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3605
DOI: 10.1128/jvi.01778-21
Resource: (BDSC Cat# 3605,RRID:BDSC_3605)
Curator: @mpairish
SciCrunch record: RRID:BDSC_3605
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- Jun 2024
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www.theguardian.com www.theguardian.com
- May 2024
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www.liberation.fr www.liberation.fr
- Apr 2024
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www.derstandard.at www.derstandard.at
- Mar 2024
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www.theguardian.com www.theguardian.com
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shopgoodwill.com shopgoodwill.com
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Olivetti Underwood 21
2024-03-01<br /> $67.99 sale<br /> https://shopgoodwill.com/item/192324052
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www.nytimes.com www.nytimes.com
- Feb 2024
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www.repubblica.it www.repubblica.it
- Jan 2024
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www.handelsblatt.com www.handelsblatt.com
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Erläuterungen der Energy Charts des Fraunhofer-Instituts für solare Energiesysteme: 2023 kamen 55% des deutschen Strombedarfs aus erneuerbaren Energien, die Kohleverstromung ist deutlich gesunken. Der Verzicht auf Atomkraftwerke war keine Ursache dafür, dass Deutschland in diesem Jahr mehr Strom importierte als exportierte. https://www.handelsblatt.com/unternehmen/energie/strommix-deutschland-so-abhaengig-ist-deutschland-von-stromimporten/100002618.html
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www.derstandard.de www.derstandard.de
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Der Standard informiert über zwei Studien, die unter anderem den Zusammenhang von Stadtentwicklung und globaler Erhitzung betreffen. Vor allem in den USA wird für die Mehrzahl der großen Städte eine Schrumpfung vorausgesagt, verstärkt durch die Folgen der Erhitzung. Die Studie verwendet ist Szenarien des IPCC. Unter anderem ergibt sich daraus die Forderung, die Stadtplanung nicht mehr vor allem an der Vorstellung von wachsenden Städten auszurichten. https://www.derstandard.de/story/3000000203501/tausende-us-staedte-koennten-bis-2100-zu-geisterorten-werden
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www.derstandard.at www.derstandard.at
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Die österreichischen CO2-Emissionen sind 2023 um etwa 7% gesunken. EU-weit sind die Emissionen um ca 7,4% gefallen, in den USA um 3%. In Indien und China sind sie gestiegen.
Bericht des Wegener Centers: https://wegccloud.uni-graz.at/s/k4DxDgJTKn68QjL
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- Dec 2023
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www.nytimes.com www.nytimes.com
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www.liberation.fr www.liberation.fr
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Eine neue französische Studie beschäftigt sich mit einer Folge des dramatischen Rückgangs der Insekt. Mangels bestäubender Insekten befruchten sich Pflanzen selbst. Dadurch werden die nächsten Generationen kleiner und liefern weniger Nektar. Es kommt zu einem Rückkopplungseffekt, weil so noch weniger Insekten überleben. In dem Interview mit der liberation erklärt der Biologe diesen Effekt als Beispiel für eine unkontrollierbare und nicht mehr zurücknehmbare, von Menschen ausgelöste Entwicklung. https://www.liberation.fr/environnement/biodiversite/pollinisation-et-disparition-des-insectes-nous-sommes-dans-une-spirale-incontrolable-20231221_CV6BZLN2WJANLN2A2EFUGCJLZI/
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www.derstandard.de www.derstandard.de
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www.theguardian.com www.theguardian.com
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www.theguardian.com www.theguardian.com
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www.theguardian.com www.theguardian.com
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www.theguardian.com www.theguardian.com
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www.theguardian.com www.theguardian.com
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www.derstandard.de www.derstandard.de
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paultough.com paultough.com
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They also express concern that students without the deep and broad background knowledge and fluency that affluent children generally absorb from their homes and communities first need to develop that core knowledge before they can benefit from a collaborative, project-based approach.
This is an interesting point - can you not profit from PBL if you haven't had the lived experiences of a more privileged studennt?
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Research on both inequality across schools and tracking within schools has suggested that students in more affluent schools and top tracks are given the kind of problem-solving education that befits the future managerial class, whereas students in lower tracks and higher-poverty schools are given the kind of rule-following tasks that mirror much of factory and other working-class work.”
These insights are based on the research of Jean Anyon. She collected data in 5 different schools, that served particular social classes and noted the differences in how students are taught, based on the social class the school served.
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When most of our current pedagogical practices were developed more than a century ago, the essential economic purpose of public schools was to produce industrial workers who were fast and reliable when assigned repetitive mechanical or clerical tasks. In this century, deeper-learning proponents argue, the job market requires a very different set of skills, one that our current educational system is not configured to help students develop: the ability to work in teams, to present ideas to a group, to write effectively, to think deeply and analytically about problems, to take information and techniques learned in one context and adapt them to a new and unfamiliar problem or situation.
Important point - the way that we have traditionally taught are a reflection of a different world. Yes...there has been that little evolution in how we teach!
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student-centered learning
this is a contrast to teacher-centered practices
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But students in schools serving mostly low-income children were almost all (91 percent) in classrooms marked by basic, uninteresting teaching.
Think back to your SL school's SARC report - is you school serving mostly low-income students?
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And while the Science authors found instruction to be basic and repetitive even in American schools with a mostly middle-class or upper-middle-class student population, they found that the situation was considerably worse in schools that enrolled a lot of low-income children.
How do you think this finding impacts other aspects of classroom life - like motivation and classroom management?
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Pianta’s researchers found that in almost every school they observed, the instruction students received was repetitive and undemanding, limited mostly to the endless practice of basic skills.
This is depressing. Do you feel that this is true about the instruction that you observed in your SL placement?
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21. Challenge
I wonder how this is related to persistance.
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- Nov 2023
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In den Niederlanden wird mit einem Wahlsieg von Parteien gerechnet, die für klimapolitischen Stillstand oder Rückschritt stehen. Die taz beschreibt den Stand der Auseinandersetzungen. Hervorgehoben wird die Stärke der Klimabewegung und die Klimarechtssprechung. Für die meisten Wähler:innen sei Klimapolitik nicht von Verteilungs- und Gerechtigkeitsfragen zu trennen.https://taz.de/Wahlen-in-den-Niederlanden/!5971214/
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www.theguardian.com www.theguardian.com
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Roger Hardy erklärt in diesem Artikel über die von ihm in Großbritannien gegründete Organisation Round our Way, dass Arbeiterklassen-Communities von der globalen Erhitzung und ihren Folgen besonders stark betroffen sind und das auch wissen. Nur eine Klimabewegung für "ordinary people" könne das Fundament für einen gesellschaftlichen Konsens über Klimaschutz herstellen. https://www.theguardian.com/environment/commentisfree/2023/nov/21/working-class-people-climate-crisis-policy
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www.derstandard.de www.derstandard.de
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61% der Österreicher:innen sprachen sich in einer von Greenpeace in Auftrag gegebenen Umfrage für ein Verbot von Privatflügen aus, 79% für eine Kerosinsteuer, 70% für ein Verbot von Kurzstreckenflügen, wenn alternativ mit der Bahn gefahren werden kann. https://www.derstandard.de/story/3000000196060/mehrheit-in-oesterreich-fuer-privatjet-verbot-und-kerosinsteuer
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www.derstandard.de www.derstandard.de
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www.theguardian.com www.theguardian.com
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www.liberation.fr www.liberation.fr
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Bericht der International Energy Agency zur Reduktion der Methanemissionen bei der Förderung von Öl und Gas. Trotz der Rekordgewinne unternehmen die fossilen Energiefirmen bei weitem nicht genug, um diese Emissionen zu stoppen. Nur 3% der Gewinne des letzten Jahres wären nötig um das Methan, das bei der Öl- und Gasförderung in die Atmosphäre entweicht, um 75% zu reduzieren.
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- Oct 2023
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www.nytimes.com www.nytimes.com
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Here is Alter’s version of the well-known opening of Genesis 21, part of the story of Isaac, the miracle baby of 90-year-old Sarah, and her 99-year-old husband, Abraham: “And the Lord singled out Sarah.” The word Alter is translating as “singled out” is pakad. The King James, and most others after it, translate it as “visited.” The Jewish Publication Society has it as “remembered.” Others translate it as “kept his word,” “took note of,” “was gracious to,” “was attentive to” or “blessed.” A good literal version, provided by the canny contemporary translator Everett Fox, has it as “took account of” — and there is something numerical and even administrative about pakad. (Elsewhere in the Bible, in the context of describing a public census, pakad means “to number”; in modern Hebrew, it is related to the words for “officer,” “clerk” and “roll-call.”) Weaving together its numerical dimensions with a thread of bureaucratic banality, Alter yields the anxious verb “singled out” and with it, reveals new layers of tension in this story.
translation of pakad, an administrative word literally translated as "took account of" as "took note of"
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www.theparrotandtheigloo.com www.theparrotandtheigloo.com
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https://www.theparrotandtheigloo.com/
The endnotes missing from the book.
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www.liberation.fr www.liberation.fr
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In Frankreich ersetzt die Eisenbahn auf Inlandsstrecken zunehmend das Flugzeug. Flüge sind verboten, wenn eine Eisenbahnverbindung unter 2:30 Stunden Dauer möglich ist. Da die Fahrpreise abhängig von der Zahl der NutzerInnen bestimmt werden, werden bestimmte Verbindungen für viele unerschwinglich. https://www.liberation.fr/economie/transition-ecologique-en-concurrence-avec-lavion-le-ferroviaire-espere-ramener-les-passagers-sur-terre-20231021_PF5A4OJLS5HAVBNZHMIJ52NHII/
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Annotators
URL
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en.wikipedia.org en.wikipedia.org
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https://en.wikipedia.org/wiki/Shmita
During shmita, the land is left to lie fallow and all agricultural activity, including plowing, planting, pruning and harvesting, is forbidden by halakha (Jewish law).
The sabbath year (shmita; Hebrew: שמיטה, literally "release"), also called the sabbatical year or shǝvi'it (שביעית, literally "seventh"), or "Sabbath of The Land", is the seventh year of the seven-year agricultural cycle mandated by the Torah in the Land of Israel and is observed in Judaism.
Tags
- 2 Kings 19:29
- remission year
- Exodus 23:10-11
- agriculture
- Nehemiah 10:31
- Jewish law
- time in relation to work
- Isaiah 37:30
- Deuteronomy 15:1-6
- sabbaticals
- shmita
- Jeremiah 34:13-14
- jubilee
- halakha
- Deuteronomy 31:10-13
- debt
- Friends of the Link 2023-10-18
- Leviticus 25:5
- 2 Chronicles 36:20-21
- Leviticus 25
Annotators
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ucscollections.wordpress.com ucscollections.wordpress.com
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Proverbs 21:5 New Living Translation, “Good planning and hard work lead to prosperity, but hasty shortcuts lead to poverty.”
similar to plan your work and work your plan
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- Sep 2023
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www.repubblica.it www.repubblica.it
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www.nytimes.com www.nytimes.com
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Den Angaben der amerikanischen Wetterbeobachtungsorganisation NOAA zufolge war der vergangene August der wärmste der Messgeschichte. Die Oberflächentemperatur lag im Durchschnitt 1,25° über dem vorindustriellen Mittel. Die Oberflächentemperatur der Ozeane lag mit dem.August 5 Monate in Folge auf Rekordniveau. https://www.nytimes.com/2023/09/21/climate/hottest-august-on-record.html
NOAA-Bericht: https://www.noaa.gov/news/world-just-sweltered-through-its-hottest-august-on-record
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www.nytimes.com www.nytimes.com
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Die Biden-Administration hat alle amerikanischen Bundesbehörden angewiesen, bei allen Projekten die Kosten, die durch die globale Erhitzung verursacht werden, mit zu budgetieren. Damit wird eine bisher schon von der Umweltbehörde EPA verwendete Metrik der "social costs of carbon" auf die gesamte Regierungstätigkeit ausgeweitet.Mit einem Budget von ungefähr 600 Milliarden Dollar im Jahr ist die amerikanische Bundesregierung der größte Verbraucher von Gütern und Dienstleistungenn in der Welt. https://www.nytimes.com/2023/09/21/climate/biden-climate-change-economic-cost.html
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Al Gore hat bei einem Event den New York Times gesagt, dass die Fossilindustrie versucht, den UN-Dekarbonisierungsprozess zu kapern.. Dazu gehöre die Strategie der Vereinten Arabischen Emirate, sich als Vorkämpferland für erneuerbare Energien darzustellen und tatsächlich die Abhängigkeit von fossilen Energie noch zu vergrößern. https://www.nytimes.com/2023/09/21/climate/al-gore-fossil-fuel.html
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www.nytimes.com www.nytimes.com
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Bei der Mehrheit der großen Konzerne des Food- und Restaurantsektors steigen die Emissionen obwohl diese Firmen sich zur Dekarbonisierung verpflichtet haben. Diese Ziele lassen sich aber nicht mit den Wachstumsstrategien der Unternehmen vereinbar machen. Dabei ist die Emissionsintensität der Fleisch- und Weizenproduktion der wichtigste Faktor. https://www.nytimes.com/2023/09/22/business/food-companies-emissions-climate-pledges.html
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www.liberation.fr www.liberation.fr
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saragoldrickrab.com saragoldrickrab.com
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en.wikipedia.org en.wikipedia.org
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Abomination of desolation
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- Aug 2022
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twitter.com twitter.com
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Pouria Hadjibagheri [@Pouriaaa]. (2021, July 14). The state of the UK’s statistical system 2020/21 by @StatsRegulation Thank you! 🎊😀🎉 See the report: Https://osr.statisticsauthority.gov.uk/publication/the-state-of-the-uks-statistical-system-2020-21/pages/8/ https://t.co/dEBmVz3oTm [Tweet]. Twitter. https://twitter.com/Pouriaaa/status/1415371346775838725
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- May 2022
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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DICER1 syndrome encompasses a variety of benign and malignant manifestations including multinodular goitre
Gene: DICER1 PMCID: PMC8451242 PMID: 34552563 Pathogenic Inheritance Pattern: Autosomal Dominant MultipleDiseaseEntities Disease Entity: DICER1 syndrome, multinodular goitre, cystic nephroma, anaplastic renal sarcoma, Wilms tumour, differentiated thyroid carcinoma, gynandroblastoma, ciliary body medulloepithelioma, embryonal rhabdomyosarcoma, pineoblastoma, pituitary blastoma, kidney cyst, pulmonary cyst, Sertoli-Leydig Cell Tumor. Mutation: Germline MultipleGeneVariants Variant & Clinvar IDs: c.3452_3453del (485534), c.316del (no ClinVar ID), c.171_172insAC (no ClinVar ID), c.3434del (no ClinVar ID), c.988C>T (933007), c.5388dup (no ClinVar ID) Zygosity: None provided. Case: At time of operation, the goitre patients living in Denmark were ages 21, 12, 21, 8, 14, and 16. Four underwent total thyroidectomies, and two underwent partial thyroidectomies. The patient originally aged 21 previously had a kidney cyst at age 14 and a pulmonary cyst at an unknown age. The patient aged 14 at time of partial thyroidectomy later manifested a Sertoli-Leydig Cell Tumor at age 15. All six patients were female. CasePresentingHPO: None provided. CasePreviousTesting: thyroidectomy gnomAD: ENSG00000100697.10, https://gnomad.broadinstitute.org/gene/ENSG00000100697 Mutation Type: Frameshift, Nonsense
Tags
- cancer
- Age:8
- ClinvarID:933007
- Pathogenic
- c.3434del
- Age:12
- PMCID:PMC8451242
- AutosomalDominant
- female
- Cohort
- DICER1
- Germline
- ClinvarID:NotProvided
- MultipleDiseaseEntities
- Mutation:Frameshift
- Age:21
- MultipleGeneVariants
- Gene:DICER1
- goitre
- Age:14
- goiter
- thyroidectomy
- Frameshift
- PMID:34552563
- c.5388dup
- gnomAD:ENSG00000100697.10
- c.171_172insAC
- c.988C>T
- Mutation:Nonsense
- ClinvarID:485534
- Nonsense
- c.3452_3453del
- c.316del
Annotators
URL
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- Dec 2021
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www.thehindu.com www.thehindu.com
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Majority Act, 1875
- Age 18 is age of majority
- 21years if guardian
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- Aug 2021
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www.cdc.gov www.cdc.gov
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Bixler, D. (2020). SARS-CoV-2–Associated Deaths Among Persons Aged 21 Years — United States, February 12–July 31, 2020. MMWR. Morbidity and Mortality Weekly Report, 69. https://doi.org/10.15585/mmwr.mm6937e4
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- Jul 2021
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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scn1labs552
DOI: 10.1523/ENEURO.0066-17.2017
Resource: (ZFIN Cat# ZDB-GENO-040720-21,RRID:ZFIN_ZDB-GENO-040720-21)
Curator: @mpairish
SciCrunch record: RRID:ZFIN_ZDB-GENO-040720-21
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- Mar 2021
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Results for individual PALB2 variants were normalized relative to WT-PALB2 and the p.Tyr551ter (p.Y551X) truncating variant on a 1:5 scale with the fold change in GFP-positive cells for WT set at 5.0 and fold change GFP-positive cells for p.Y551X set at 1.0. The p.L24S (c.71T>C), p.L35P (c.104T>C), p.I944N (c.2831T>A), and p.L1070P (c.3209T>C) variants and all protein-truncating frame-shift and deletion variants tested were deficient in HDR activity, with normalized fold change <2.0 (approximately 40% activity) (Fig. 1a).
AssayResult: 4.4
AssayResultAssertion: Indeterminate
StandardErrorMean: 0.09
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A total of 84 PALB2 patient-derived missense variants reported in ClinVar, COSMIC, and the PALB2 LOVD database were selected
HGVS: NM_024675.3:c.1847A>G p.(Asp616Gly)
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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SUPPLEMENTARY DATA
AssayResult: 109.2
AssayResultAssertion: Not reported
PValue: > 0.9999
Comment: Exact values reported in Table S3.
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To this end, 44 missense variants found in breast cancer patients were identified in the ClinVar database (https://www.ncbi.nlm.nih.gov/clinvar) and/or selected by literature curation based on their frequency of description or amino acid substitution position in the protein (Supplemental Table S1).
HGVS: NM_024675.3:c.3073G>A p.(Ala1025Thr)
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www.nature.com www.nature.com
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Source Data
AssayResult: 74.36
AssayResultAssertion: Not reported
ReplicateCount: 2
StandardErrorMean: 1.89
Comment: Exact values reported in “Source Data” file.
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Source Data
AssayResult: 84.43
AssayResultAssertion: Not reported
ReplicateCount: 2
StandardDeviation: 2.77
StandardErrorMean: 1.96
Comment: Exact values reported in “Source Data” file. Discrepancy in “Source Data” file: protein reported as L855P (based on matching values reported in the “Supplementary Data 1” file to values reported in the “Source Data” file.
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We, therefore, analyzed the effect of 48 PALB2 VUS (Fig. 2a, blue) and one synthetic missense variant (p.A1025R) (Fig. 2a, purple)29 on PALB2 function in HR.
HGVS: NM_024675.3:c.2574T>C p.(V858=)
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www.cell.com www.cell.com
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Most Suspected Brugada Syndrome Variants Had (Partial) Loss of Function
AssayResult: 21
AssayResultAssertion: Abnormal
ReplicateCount: 12
StandardErrorMean: 5.1
Comment: This variant had partial loss of function of peak current (10-50% of wildtype), therefore it was considered abnormal (in vitro features consistent with Brugada Syndrome Type 1). (Personal communication: A. Glazer)
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we selected 73 previously unstudied variants: 63 suspected Brugada syndrome variants and 10 suspected benign variants
HGVS: NM_198056.2:c.2422C>T p.(Arg808Cys)
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www.sciencemag.org www.sciencemag.org
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Wadm, Meredith, Ec. 15, 2020, and 5:00 Pm. ‘COVID-19 Is 10 Times Deadlier for People with Down Syndrome, Raising Calls for Early Vaccination’. Science | AAAS, 15 December 2020. https://www.sciencemag.org/news/2020/12/covid-19-10-times-deadlier-people-down-syndrome-raising-calls-early-vaccination.
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docs.google.com docs.google.com
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Slides from Hypothes.is presentation at OLC Innovate
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- Feb 2021
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jmg.bmj.com jmg.bmj.com
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Supplemental material
AssayResult: 60
AssayResultAssertion: Normal
Comment: See Table S2 for details
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Supplemental material
AssayResult: 8.6
AssayResultAssertion: Abnormal
Comment: See Table S2 for details
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We analysed a total of 82 blood samples derived from 77 individuals (online supplemental table 3). These 77 individuals corresponded either to new index cases suspected to harbour a pathogenic TP53 variant or to relatives of index cases harbouring TP53 variants.
HGVS: NM_000546.5:c.491_494del p.(Lys164Serfs*5)
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cubicmuse.com cubicmuse.com
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By focusing on the condition of the looking glass, Joyce suggests the artist does not start his work with a clean slate. Rather there is considerable baggage he or she must overcome. This baggage might include colonial conditions or biased assumptions. Form and context influence content.
This seems a bit analogous to Peggy McIntosh's Backpack of White Privilege I was looking at yesterday.
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www.buzzfeed.com www.buzzfeed.com
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www.youtube.com www.youtube.com
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Privilege
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myemail.constantcontact.com myemail.constantcontact.com
- Jan 2021
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www.theguardian.com www.theguardian.com
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Hintergrund zu Bidens Klimapolitik, internationale Erwartungen.
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Even before Mr. Biden was inaugurated on Wednesday, some Republicans lashed out against his new policy direction.
Bericht über die ersten Executive Orders Bidens zum Klimanotstand und über die Widerstände dagegen.
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- Sep 2020
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZFIN_ZDB-GENO-100415-21
DOI: 10.7554/eLife.53761
Resource: ZFIN_ZDB-GENO-100415-21
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-GENO-100415-21
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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ZFIN ID: ZDB-ALT-070420–21
DOI: 10.7554/eLife.54937
Resource: (ZFIN Cat# ZDB-ALT-070420-21,RRID:ZFIN_ZDB-ALT-070420-21)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-ALT-070420-21
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- Dec 2019
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www.journaldemontreal.com www.journaldemontreal.com
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La loi ne vise donc pas à interdire l’accès à ces postes pour les chrétiens, les juifs, les musulmans.
Il manque des prémisses à cette conclusion.
Bien sûr, la loi ne vise pas directement à interdire l’accès aux postes pour les chrétiens, les juifs, les musulmans (mais dans les faits, c'est un peu ce qui arrive, visé ou non).<br> Voyons comment on y arriverait.
Décortiquons l'argument :
- La loi interdit le port des signes religieux au travail.
- Les principales religions au Québec n’obligent pas le port de signes religieux au travail.
- Si la religion oblige une personne de porter un signe religieux, cette personne n'a pas accès au travail (<mark>IMPLICATION</mark>).
- Les principales religions au Québec n’obligent pas le port de signes religieux (l’implication demeure vraie).
- La loi n’empêche pas l’accès au travail aux personnes religieuses (conclusion à examiner).
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les religions qui empêchent leurs fidèles d’enlever leurs signes religieux
Dérive suspecte
Il y a peut-être un glissement ici : on parle d'abord du port des signes religieux (exprimé positivement) et maintenant d’empêcher d'enlever des signes religieux (exprimé négativement).
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Ce n’est pas l’islam qui empêche les musulmanes d’enlever leur voile au travail.
- Si la constitution canadienne protège les religions, il faut respecter ce que disent les religions.
- Si c'est écrit noir sur blanc dans un texte sacré, c'est ce que dit la religion.
- La constitution canadienne protège les religions (et ce que disent les religions).
- Il n'existe aucun énoncé religieux noir sur blanc qui empêche de porter un signe religieux au travail.
- Aussi : il n'existe aucun énoncé religieux noir sur blanc qui empêche de louer un appartement à un homosexuel.
Énoncés atomiques :
C: La constitution canadienneP: Protéger les religionsS: Ce que dit un texte sacréE: Empêcher d'enlever un signe religieux au travail
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musulmans rigoristes
Rigoriste?
J'ignore si le mot sert mal le propos de R. Martineau, mais « rigoriste » qualifie bien quelqu’un qui a un attachement « très strict aux règles morales et religieuses » (donc, en toute conformité avec ce que les religions disent).
Martineau laisse croire qu’une « conception rigide » est une « conception erronée » – pas nécessairement! On peut avoir une conception très « rigide » mais rigoureusement vraie; on peut aussi avoir une conception complètement spéculative – est-ce le cas des personnes religieuses qui défendent leur droit de religion face à la loi 21?
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il ne peut invoquer la liberté de religion pour avoir le droit de refuser un appartement à un gai
Argument par l'absurde
Avec un argument par l'absurde, on peut dire n'importe quoi après avoir dit quelque chose d'absurde.
Refuser l'appartement à un homosexuel est effectivement absurde, puisqu'on doit évidemment louer l'appartement à un homosexuel. De cette contradiction, je peux dire n'importe quoi, comme dire que de ne pas employer une personne religieuse est absurde. Conclusion : il faut employer une personne ouvertement religieuse.
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conceptions crispées
Là encore, il faudrait voir ce que l’expression « conception crispée » représente (rigoriste? fortement attachée aux règles d’une religion?)
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textes sacrés
Lesquels sont justement sujets à interprétation – ce qui ne signifie pas un infini relativisme pour tout un chacun, mais que ce n’est justement pas toujours écrit noir sur blanc.
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HORS DE TOUT DOUTE
C’est bien là le problème.<br> Martineau souhaite une réponse tranchée (comme les positions qu’il <s>défend</s> pitche dans l'espace public) mais les textes sacrés sont justement sujets à une interprétation (une compréhension des textes pas forcément consensuelle), d’où plusieurs branches au sein d’une même religion.
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Pourquoi la majorité des musulmanes pratiquantes ne portent pas le voile ?
Pourquoi la minorité?
La question est rhétorique (puisqu'elle n'attend pas de réponse, drop the mic), comme si elle devait rester sans réponse (comme d'une évidence).
Cela revient simplement à mettre le fardeau sur la minorité, qui doit se justifier de ne pas appartenir à la « majorité ».
La défense des minorités représente justement le rôle essentiel du poivoir politique…
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végane extrémiste
Parlons extrêmistes
Le mot « extrémiste » (au lieu de « extrême », au bout du spectre) est assurément employé à des fins polémistes, en connotation avec le terrorisme et, accessoirement, l’islamisme (qui n’a rien à voir avec une défense correcte de l’islam, avec des pratiques violentes et proprement extrêmistes).
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- Jun 2019
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Statistical Ana(vsis
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Poly acrylamide gel electrophoresis (PAGE
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Immunoblotting
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Nucleartranslocationofp-Catenin
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Somatoplot Coordinates
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- May 2019
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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The mice were returned to a cage and were kept under a 1 OOW bulb light source to prevent hypothermia. Care was taken to ensure that the eyes are kept covered. The respiratory rate and heart rate were monitored till the mice regained complete consciousness. They were fed ab-limitum post-operatively. Metronidazole (20 mg/kg) was added to the drinking water and the mice were fed this medicated water for 5 days post-operatively. On the th post-operative day, the health of the wound was observed and the surgical clips were removed from the skin
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ost-operative care
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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The organic solution of phospholipid was made in a 1:1 mixture of chloroform and methanol at a concentration of 5 mg/ml. To get a thin and uniform lipid film, lipid was dried down onto the walls of a round bottom flask by rotating for 30 min. in a rotary evaporator fitted with a coo!;ng coil and a thermostatically controlled water bath. The temperature of the water bath was maintained 10 °C above the phase transition temperature of the respective phospholipid. The film was stored under vacuum for 1 2 h to remove the traces of the solvent after flushing with nitrogen. Subsequently, lipid film was dispersed in 30 mM Tris-HCI buffer containing O.IM NaCl, pH 7.0 at a concentration of 1 mg/ml of lipid using glass beads of 0.5 mm-3 mm in diameter. Multilamellar vesicles (ML Vs) were obtained by manual swirling for 2 h at room temperature (Bangham et al., 1965). Nitrogen was flushed into the flask and the vesicle suspension was left overnight at 4 °C. The .following day, maintaining the temperature 10 °C abcve the phase transition temperature of the corresponding phospholipid, MLVs were sonicated in a water bath sonifier (Branson 321 0) for 45 min. to get the small unilamellar vesicles (SUVs) (Huang C, 1969).
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Preparation of Lipid Vesicles
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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for 3 hours at room temperature or OIN at 4 °c. The excess antibody was washed by washing the filters with PBST for 15 minutes with at least three changes. The filters were subsequently incubated in the appropriate dilution of the second antibody for one hour at room temperature. Dilutions of the primary as well as secondary antibody were made in 3 % BSA in PBST. Following incubation with the second antibody, the filters were washed vigorously with PBST for 5 minutes. The washing was repeated 5-6 times. Finally, the filters were washed twice in PBS and colour developed with DAB ( 0.5 mg I ml in PBS ) containing 6 ul I 10 ul of 30 % H2o2. The colour reaction was stopped after 5 -10 minutes by washing the filters with distilled water.
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apparatus. At the end of the run, the gel was equilibrated for 15 minutes in the transfer buffer ( 25 mM Tris base,, 192 mM glycine, 20% V/V methanol). Immunoblotting was performed essentially as described by Towbin et al., 1979 ) . The proteins-were blotted on to nitrocellulose Schleicher and Schuell ) for 16 hours in LKB Transphor apparatus. After the completion of electric transfer of proteins, the nitrocellulose paper was washed with PBS 10 mM sodium phosphate buffer, pH 7.4, 0.9 % saline for 10 minutes at room temperature, with gentle rocking to wash off any adhering traces of acrylamide gel. The filter was then stained with a dilute solution of amide black. This was made by diluting 5 fold with water, a solution of 0. 2 % ami do black containing 45 % v;v methanol and 10 % v;v acetic acid. The staining was done for 5 10 seconds and the filter washed immediately with distilled water. The proteins transferred to the gel could be seen at this stage. The lane containing protein molecular weight standards was cut out and . preserved by drying and storage in dark. The filter could be cut into appropriate lanes at this stage. The filter was destained by repeated washing with PBS containing 0.2 % Tween-20 ( PBST ) . After the filter destained completely, it was incubated with 3 % BSA made in PBST for 1 hour at room temperature. During this and in all the subsequent steps, incubation of the filter was performed on a rocker platform to ensure a uniform treatment. The filters were then incubated in the appropriate dilution of the primary antibody
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Electrophoretic separation of protein samples was carried out on 12.5 % SDS-PAGE in a discontinuous system as described by Laemmli ( 1970 ). The samples were reduced by boiling for 3 minutes in sample buffer containing J3-mercaptoethanol. The samples were then centrifuged for 5 minutes at 10,000 rpm to pellet down all particulate matter, prior to loading on the gel. Electrophoresis was carried out at 100 volts in a LKB vertical slab gel electrophoresis
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Western blot.
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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The method followed is essentially the same as described by Jin et al. (1992). Overnight bacterial cultures grown in minimal A medium supplemented with 0.4% glycerol and 0.5% Casamino acids with the appropriate antibiotic were subcultured 1:100 in the same medium in a volume of 20 ml (0.2% arabinose was added for induction of the plasmid-borne gene downstream of Para, wherever required) at 37 ̊C. Cultures were induced with 1 mM IPTG at A600=0.3. 1 ml samples were aliquoted at time intervals of 0 sec, 20 sec, 40 sec, 1 min, 1.5 min, 2 min, 2.5 min, 3 min, 3.5 min, 4 min, 4.5 min, 5 min, 5.5 min and 6 min into 1 ml of 0.1 mg/ml ice cold chloramphenicol and the samples were put on ice. After sampling the cultures were incubated at 37 ̊C for 15 min. 0.5 ml of this culture was then taken in duplicate tubes for β-galactosidase assays
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RNA polymerase elongation rate measurement
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Log-phase yeastcells were collected, washed and suspendedin 10 mM Tris-HCl (pH 7.5) containing 50 mg/ml zymolyase-20T. Cell suspension was incubated at room temperature and absorbance was monitored at 600 nm every10mininterval. Initial absorbance of the cultures at 0 minwas normalized to 100%and the graph was plottedas%decrease in the absorbance with respect to time
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Zymolyasedigestion assay
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Resultant precipitate was dissolved in 3 N HCl and reprecipitated in methanol:acetic acid (8:1) solution. Following 16 h incubation at room temperature, the precipitate was washed withmethanol:acetic acid (8:1) solution till green colour of the supernatant disappeared.Finally,pellet was washed thrice with methanol and air dried. Driedpellet was resuspended in 0.5 NHCl and total mannan content was quantified with phenol-sulphuric acid carbohydrate estimation method as described earlier.Commercially available purified glucose was used as the standard
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Total mannan from 3% NaOH-extractable supernatant of cell wall was precipitated by Benedict’s solution.Reducing sugars(mostly mannan) from alkali-extractable supernatant reactwith copper(II) sulphate present in Benedict’s solution and forms red copper(I) oxide precipitate.Briefly, equal volume of Benedict’s solution was added to 3% NaOH-extractable cell wall supernatant fraction and heated at 99 ̊C for 10 min
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Total mannan estimation
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Cell wall β-glucan measurement was carried out as describedpreviously with some modifications(Kapteynet.al.,2001). Briefly, cell wall fractions were washed multiple times with 1 N NaCl. Washed cell walls were boiled twice in 50 mM Tris-HCl(pH 7.8) containing 2% SDS, 100 mM Na-EDTA and 40 mM β-mercaptoethanol for 5 min to remove non-covalently linked proteins and other contaminants. SDS-treated cell wall fraction was collected and rinsed thrice with water. For β-glucan isolation, cell wallswere extracted three times, each for 1 h, in 0.5 ml 3% NaOH at 75 ̊C and centrifuged at 1,200 g.All 3% NaOH supernatant fractions were saved for isolation of mannan as described below. 3% NaOH-extractable cell wall pelletwasneutralized twice in 100 mM Tris-HCl (pH 7.5) and once in 10 mM Tris-HCl (pH 7.5) and digested with 5 mg/ml zymolyase-20T in 10 mM Tris-HCl (pH 7.5) for 14-16 h at 37 ̊C. This treatment liberates approximately 90-95% glucose into the supernatant. Total glucan content in the cell wall was measured by estimating glucose from both the solubilised supernatant and zymolyase-20T insoluble pellet fractions with phenol-sulphuric acid carbohydrate estimation method using purified glucose as the standard
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Total β-glucan estimation
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min. Cells were normalized to equal OD600, resuspendedin 1 ml 50 mM Tris-HCl (pH 7.5) and transferred to 2 ml microcentrifuge tubes. Cells were lysed with glass beadsin a homogenizer (FastPrep®-24,MP Biomedicals)asdescribed earlier.Brokencells were washed from glass beadswith 500 μl Tris-HCl (50 mM, pH 7.5) and pelleteddown at 15,000 g for 10 minto obtainall cell wall and membrane content. Pellet was then boiled for 10 minin 1mlTris-HCl(50mM; pH 7.5)solutioncontaining 2%SDS. SDS-extractable material(mannoproteins)was savedand remaining pellet wasboiled again in 500 μl Tris-HCl(50 mM; pH 7.5)buffer containing 2%SDS. Cell wallwas collectedby centrifugation at 15,000 g for 10 min, washed twice with1 ml waterandresuspendedin 100 μl 67 mM potassium phosphatebuffer. This washed cell wall materialwas used for β-glucan estimation as described below
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Yeast cell wall was isolatedas describedpreviously(De Groot et al., 2004). Briefly, cells grown underdifferent environmental conditions were harvested at 5,000 g for 5
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Crude cell wall isolation
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Cell wall isolation, zymolyasedigestion assay and β-glucan estimation
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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The quantityand purity of nucleic acids weredetermined by measuring the absorbance at 260 and 280 nmusing automated NanoDrop instrument. The concentration of nucleic acids was calculated by taking 1 OD at 260nm = 50μg/ml for DNA and 40 μg/ml for RNA. The purity of nucleic acids was checked by their A260/A280 ratio
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Spectrophotometric estimation of nucleic acids
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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A single colony of E. coli DH5α strain was inoculated in 10 mL LB medium and incubated at 37°C overnight. Overnight culture was subculturedin 250 mL of LB medium of about 0.1 OD and incubated at 18°Cfor36 htillthe OD600reached approximately0.5. Cultures were centrifuged at 2,500 x gfor 10 min at 4 ̊C and harvested cells were washed gently with 80 ml ice-cold Inoue transformation buffer(Section 2.1.6.2). Cells were collected by centrifugation at 2,500x g for 10 min at 4 ̊C and gently resuspended in 20 mLice-cold Inoue transformation buffer. To this cell suspension, 1.5 ml sterile DMSO was added and swirled gently. The cell suspension waskept on ice for 30min,and 100 μLvolume was aliquoted into pre-chilled sterile microcentrifuge tubes. Cells were immediately snap-frozen in liquid nitrogen and stored at -80°C
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Preparation of E. coli DH5α ultracompetent cells
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Ip6k1-/-(4 male, 5 female)] were exposed to 4NQO (100 μg/mL) in their drinking water. Mice were allowed free access to drinking water containing the carcinogen, and the water was changed every week.Water bottles containing carcinogen was covered with foil to avoid exposure to light. All the apparatus used for this study and remaining water were decontaminated using 1% sodiumhypochlorite for 30 min. Every week water consumption and weight of the each animal was monitored. Mice displaying any characteristics of weight loss or dehydration before 24 weeks were euthanized and examined for lesions. After 24 weeks, mice were euthanized by CO2inhalation and a complete necropsy was performed. Tissues from the aerodigestive tract (tongue, esophagus, stomach,and duodenum) werefixed in formalin and paraffin-embedded sections were stained with hematoxylin and eosin (H&E) to examine thelesions by light microscopy.Images were acquired using a bright field light microscope (Nikon ECLIPSE Ni, NIS Elements acquisition software, 20x 0.5 N.A. objective). During the pathologicalexamination, the lesions observed in the various tissues were categorized into hyperplasia, dysplasia,and invasive carcinoma. Hyperplasia was defined as an increase in the layers or thickening of the epithelium with hyperkeratinization. Dysplasia was defined as loss of epithelial cell polarity, nuclear pleomorphism and abnormal mitoses confined to the epithelium. Invasive carcinoma was defined as invasion of dysplastic cells or lesions into subepithelial tissues such as submucosa and muscle
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Carcinogenesis studies were conducted as described previously for Ip6k2-/-mice (Morrisonet al., 2009). Briefly, the carcinogen, 4NQO stock solution (5 mg/mL) was prepared fresh in propylene glycol every week. Four-week old mice [11 Ip6k1+/+(5 male, 6 female) and 9
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Carcinogenesis studies
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- Sep 2018
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primarydocuments.ca primarydocuments.ca
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In order to protect local interests, and to prevent sectional jealousies, it was found requisite that the three great divisions into which British North America is separated, should be represented in the Upper House on the principle of equality. There are three great sections, having different interests, in this proposed Confederation.
§§. 21-36 & § 91(1) of the Constitution Act, 1867
Referenced in Re: Authority of Parliament in relation to the Upper House, [1980] 1 SCR 54, 1979 CanLII 169 (SCC)
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To the Upper House is to be confided the protection of sectional interests ; therefore is it that the three great divisions are there equally represented, for the purpose of defending such interests against the combinations of majorities in the Assembly.
§§. 21-36 & § 91(1) of the Constitution Act, 1867
Referenced in Re: Authority of Parliament in relation to the Upper House, [1980] 1 SCR 54, 1979 CanLII 169 (SCC)
Tags
- Section 30 of the Constitution Act 1867
- Section 31 of the Constitution Act 1867
- Section 29 of the Constitution Act 1867
- Section 34 of the Constitution Act 1867
- Section 25 of the Constitution Act 1867
- Section 27 of the Constitution Act 1867
- Section 33 of the Constitution Act 1867
- Section 91(1) of the Constitution Act 1867
- Section 36 of the Constitution Act 1867
- Section 22 of the Constitution Act 1867
- Section 35 of the Constitution Act 1867
- Section 28 of the Constitution Act 1867
- Section 26 of the Constitution Act 1867
- Section 23 of the Constitution Act 1867
- Section 21 of the Constitution Act 1867
- Section 24 of the Constitution Act 1867
- Section 32 of the Constitution Act 1867
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primarydocuments.ca primarydocuments.ca
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But the very essence of our compact is that the union shall be federal and not legislative. Our Lower Canada friends have agreed to give us representation by population in the Lower House, on the express condition that they shall have equality in the Upper House. On no other condition could we have advanced a step ; and, for my part, I am quite willing they should have it. In maintaining the existing sectional boundaries and handing over the control of local matters to local bodies, we recognize, to a certain extent, a diversity of interests ; and it was quite natural that the protection for those interests, by equality in the Upper Chamber, should be demanded by the less numerous provinces.
§§. 21-36 & § 91(1) of the Constitution Act, 1867
Referenced in Re: Authority of Parliament in relation to the Upper House, [1980] 1 SCR 54, 1979 CanLII 169 (SCC)
Tags
- Section 30 of the Constitution Act 1867
- Section 31 of the Constitution Act 1867
- Section 29 of the Constitution Act 1867
- Section 34 of the Constitution Act 1867
- Section 25 of the Constitution Act 1867
- Section 27 of the Constitution Act 1867
- Section 33 of the Constitution Act 1867
- Section 91(1) of the Constitution Act 1867
- Section 36 of the Constitution Act 1867
- Section 22 of the Constitution Act 1867
- Section 35 of the Constitution Act 1867
- Section 28 of the Constitution Act 1867
- Section 26 of the Constitution Act 1867
- Section 23 of the Constitution Act 1867
- Section 21 of the Constitution Act 1867
- Section 24 of the Constitution Act 1867
- Section 32 of the Constitution Act 1867
Annotators
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- Jul 2018
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Local file Local file
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14
Step 20:
Flip the frame over and align the legs with the frame as shown in the graph.
Step 21:
Insert the legs into the frame.
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- Jul 2017
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www.dailysabah.com www.dailysabah.com
- Apr 2017
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www.ctvnews.ca www.ctvnews.ca
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Cancer cases expected to soar 40 per cent by 2030
This article helps me think about the population in 15 years and what kinds of services we'll need to provide.
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- Mar 2017
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magazine.pianoperformers.org magazine.pianoperformers.org
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climate.nasa.gov climate.nasa.gov
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Mon Mar 27 2017 09:20:19 GMT-0400 (Eastern Daylight Time)
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Annotators
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www.hotnews.ro www.hotnews.ro
- Sep 2016
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www.nature.com www.nature.com
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educators could use annotation to build interactive classroom lessons
It's actually a very good idea to ask students to comment their readings and to share their comments.
Can be used in traditional or flipped classrooms.
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