- Sep 2024
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www-nature-com.ezproxy.rice.edu www-nature-com.ezproxy.rice.edu
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we found that the clouds can become more compact and produce substantially less rain upon: (i) reducing the ramp rate to 1 °C/sec in every PCR step (Fig. 10a), (ii) increasing the annealing/extension time to 2 minutes (up from 1 minute; Fig. 10b), and (iii) increasing the number of cycles to 50 (Fig. 10c)28
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- May 2024
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www.bio-rad.com www.bio-rad.com
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Restriction Digestion
See notes in this section for restriction digestion tips
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www.bio-rad.com www.bio-rad.com
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DNA fragmentation by restriction digestion prior to dropletgeneration enables optimal accuracy by separating tandemgene copies, reducing sample viscosity, and improving templateaccessibility for input samples >66 ng per well
NEB says
Digestion is recommended whenever DNA input is greater than 75 ng Source
NEB says that biorad recommends these enzymes: AluI, CviQI, HaeIII, HindIII-HF, MseI
More guidelines
- Assemble ddPCR reactions at room temperature, this allows the restriction enzyme to digest the gDNA during the reaction set-up period
- Prepare reaction mixes as you would for a standard ddPCR reaction. Add 0.5–1 μL of each restriction enzyme (5–20 units, depending on enzyme concentration) to the reaction mixture
- After set-up, simply continue droplet generation as normal
- Restriction enzyme will be inactivated during first PCR denaturation step
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Refer to this protocol mentioned in the BioRad page, annotation
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- Feb 2023
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Digestion is recommended whenever DNA input is greater than 75 ng
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- Nov 2022
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www.biosearchtech.com www.biosearchtech.com
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For those shorter than 25 bases, a standard (dual labeled) BHQ™ Probe may be perfectly satisfactory.
Nova might be better to have for 1-step ddPCR applications where the BHQ quenchers are unstable due to reduction of the N=N azo bond by the DTT additive
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- Jan 2022
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virologydownunder.com virologydownunder.com
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Mackay, A. I. M. & PhD. (2022, January 29). Thank goodness we did all the work. Virology Down Under. https://virologydownunder.com/thank-goodness-we-did-all-the-work/
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Angel, D. M., Gao, D., DeLay, K., Lin, E. Z., Eldred, J., Arnold, W., Santiago, R., Redlich, C., Martinello, R. A., Sherman, J. D., Peccia, J., & Godri Pollitt, K. J. (2022). Development and Application of a Polydimethylsiloxane-Based Passive Air Sampler to Assess Personal Exposure to SARS-CoV-2. Environmental Science & Technology Letters. https://doi.org/10.1021/acs.estlett.1c00877
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twitter.com twitter.com
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ɪᴀɴ ᴍ. ᴍᴀᴄᴋᴀʏ, ᴘʜᴅ 🦠🤧🧬🥼🦟🧻🧙♂️. (2021, December 25). This is much better. #COVIDisAirborne https://who.int/news-room/questions-and-answers/item/coronavirus-disease-covid-19-how-is-it-transmitted https://t.co/ScaJHRRwVa [Tweet]. @MackayIM. https://twitter.com/MackayIM/status/1474706710090579970
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- Nov 2021
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www.science.org www.science.org
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Wang, C. C., Prather, K. A., Sznitman, J., Jimenez, J. L., Lakdawala, S. S., Tufekci, Z., & Marr, L. C. (n.d.). Airborne transmission of respiratory viruses. Science, 373(6558), eabd9149. https://doi.org/10.1126/science.abd9149
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Jose-Luis Jimenez on Twitter. (n.d.). Twitter. Retrieved 5 November 2021, from https://twitter.com/jljcolorado/status/1435977594302713858
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- Jul 2021
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bmcinfectdis.biomedcentral.com bmcinfectdis.biomedcentral.com
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Escandón, K., Rasmussen, A. L., Bogoch, I. I., Murray, E. J., Escandón, K., Popescu, S. V., & Kindrachuk, J. (2021). COVID-19 false dichotomies and a comprehensive review of the evidence regarding public health, COVID-19 symptomatology, SARS-CoV-2 transmission, mask wearing, and reinfection. BMC Infectious Diseases, 21(1), 710. https://doi.org/10.1186/s12879-021-06357-4
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- Apr 2021
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www.thelancet.com www.thelancet.com
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Greenhalgh, Trisha, Jose L. Jimenez, Kimberly A. Prather, Zeynep Tufekci, David Fisman, and Robert Schooley. ‘Ten Scientific Reasons in Support of Airborne Transmission of SARS-CoV-2’. The Lancet 0, no. 0 (15 April 2021). https://doi.org/10.1016/S0140-6736(21)00869-2.
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Swenson, A. (2021, April 20). Study lacks evidence on masks, isn’t linked to Stanford. AP NEWS. https://apnews.com/article/fact-checking-629043235973
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- Feb 2021
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advances.sciencemag.org advances.sciencemag.org
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Mathai, V., Das, A., Bailey, J. A., & Breuer, K. (2021). Airflows inside passenger cars and implications for airborne disease transmission. Science Advances, 7(1), eabe0166. https://doi.org/10.1126/sciadv.abe0166
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WABC. ‘Coronavirus: Glasses Wearers Less Likely to Get COVID, Study Says’. ABC7 New York, 24 February 2021. https://abc7ny.com/10365580/.
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- Oct 2020
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academic.oup.com academic.oup.com
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Landi, F., Marzetti, E., Sanguinetti, M., Ciciarello, F., Tritto, M., Benvenuto, F., Bramato, G., Brandi, V., Carfì, A., D’Angelo, E., Fusco, D., Lo Monaco, M. R., Martone, A. M., Pagano, F., Rocchi, S., Rota, E., Russo, A., Salerno, A., Cattani, P., … Bernabei, on behalf of the G. A. C.-19 G. T. (n.d.). Should face masks be worn to contain the spread of COVID-19 in the postlockdown phase? Transactions of The Royal Society of Tropical Medicine and Hygiene. https://doi.org/10.1093/trstmh/traa085
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- Sep 2020
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www.thelancet.com www.thelancet.com
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Bax, A., Bax, C. E., Stadnytskyi, V., & Anfinrud, P. (2020). SARS-CoV-2 transmission via speech-generated respiratory droplets. The Lancet Infectious Diseases, 0(0). https://doi.org/10.1016/S1473-3099(20)30726-X
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jamanetwork.com jamanetwork.com
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Luo, Z., Li, S., Li, N., Li, Y., Zhang, Y., Cao, Z., & Ma, Y. (2020). Assessment of Pediatric Outpatient Visits for Notifiable Infectious Diseases in a University Hospital in Beijing During COVID-19. JAMA Network Open, 3(8), e2019224–e2019224. https://doi.org/10.1001/jamanetworkopen.2020.19224
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www.abc.net.au www.abc.net.au
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The tricks of using an aeroplane toilet and other ways to reduce your COVID risk while flying. (2020, August 28). https://www.abc.net.au/news/health/2020-08-29/coronavirus-transmission-flights/12593970
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- Aug 2020
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www.npr.org www.npr.org
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Can Air Conditioners Spread COVID-19? (n.d.). NPR.Org. Retrieved August 22, 2020, from https://www.npr.org/sections/goatsandsoda/2020/08/15/897147164/can-air-conditioners-spread-covid-19
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aip.scitation.org aip.scitation.org
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Verma, S., Dhanak, M., & Frankenfield, J. (2020). Visualizing the effectiveness of face masks in obstructing respiratory jets. Physics of Fluids, 32(6), 061708. https://doi.org/10.1063/5.0016018
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pubs.acs.org pubs.acs.org
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Shan, B., Broza, Y. Y., Li, W., Wang, Y., Wu, S., Liu, Z., Wang, J., Gui, S., Wang, L., Zhang, Z., Liu, W., Zhou, S., Jin, W., Zhang, Q., Hu, D., Lin, L., Zhang, Q., Li, W., Wang, J., … Haick, H. (2020). Multiplexed Nanomaterial-Based Sensor Array for Detection of COVID-19 in Exhaled Breath. ACS Nano. https://doi.org/10.1021/acsnano.0c05657
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Carey, B., & Glanz, J. (2020, July 30). Aboard the Diamond Princess, a Case Study in Aerosol Transmission. The New York Times. https://www.nytimes.com/2020/07/30/health/diamond-princess-coronavirus-aerosol.html
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advances.sciencemag.org advances.sciencemag.org
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Fischer, E. P., Fischer, M. C., Grass, D., Henrion, I., Warren, W. S., & Westman, E. (2020). Low-cost measurement of facemask efficacy for filtering expelled droplets during speech. Science Advances, eabd3083. https://doi.org/10.1126/sciadv.abd3083
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- Jul 2020
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www.nature.com www.nature.com
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Lewis, D. (2020). Mounting evidence suggests coronavirus is airborne—But health advice has not caught up. Nature, 583(7817), 510–513. https://doi.org/10.1038/d41586-020-02058-1
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bahri, muhamad. (2020). The nexus impacts of the Covid-19: A qualitative perspective [Preprint]. SocArXiv. https://doi.org/10.31235/osf.io/yj8c9
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www.abc.net.au www.abc.net.au
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Coronavirus spreads through the air, 230 scientists write in open letter to WHO. (2020, July 6). https://www.abc.net.au/news/2020-07-06/aerosol-transmission-of-covid-19/12425852
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www.nationalgeographic.com www.nationalgeographic.com
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See how a sneeze can launch germs much farther than 6 feet. (2020, April 17). Science. https://www.nationalgeographic.com/science/2020/04/coronavirus-covid-sneeze-fluid-dynamics-in-photos/
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www.nytimes.com www.nytimes.com
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Mandavilli, A. (2020, July 4). 239 Experts With One Big Claim: The Coronavirus Is Airborne. The New York Times. https://www.nytimes.com/2020/07/04/health/239-experts-with-one-big-claim-the-coronavirus-is-airborne.html
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Flushing “can propel viral infection 3ft into air.” (2020, June 16). BBC News. https://www.bbc.com/news/health-53047819
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- Jun 2020
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royalsociety.org royalsociety.org
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DELVE group publishes evidence paper on the use of face masks in tackling Coronavirus (COVID-19) pandemic | Royal Society. (2020 May 04). https://royalsociety.org/news/2020/05/delve-group-publishes-evidence-paper-on-use-of-face-masks/
Tags
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Annotators
URL
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- May 2020
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www.pnas.org www.pnas.org
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Stadnytskyi, V., Bax, C. E., Bax, A., & Anfinrud, P. (2020). The airborne lifetime of small speech droplets and their potential importance in SARS-CoV-2 transmission. Proceedings of the National Academy of Sciences, 202006874. https://doi.org/10.1073/pnas.2006874117
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theconversation.com theconversation.com
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Ciric, L. (2020 April 29). Coronavirus shopping tips to keep you safe at the supermarket. The Conversation. http://theconversation.com/coronavirus-shopping-tips-to-keep-you-safe-at-the-supermarket-137013
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- Apr 2020
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www.nature.com www.nature.com
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Leung, N.H.L., Chu, D.K.W., Shiu, E.Y.C. et al. Respiratory virus shedding in exhaled breath and efficacy of face masks. Nat Med (2020). https://doi.org/10.1038/s41591-020-0843-2
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