45 Matching Annotations
  1. Last 7 days
    1. We know the audience for such games is limited. In order for us to produce games up to our standards, we rely on a direct sales model. Our games are not designed for traditional distribution or retail channels. The vast majority of all copies produced will be sent to Kickstarter backers or to people who purchase games through our store.  This means we can spend many more resources on the game's physical production without having to worry about retail viability.
  2. Apr 2021
    1. bacteria10

      Only intracellular pathogen bacteria infecting mammalian cells has been reported in 10. Would be interesting to see if this works for a culture of E. coli

    1. To explore the mentor–mentee relationship with a focus on determining the characteristics of effective mentors and mentees and understanding the factors influencing successful and failed mentoring relationships.

      straight to the point

  3. Mar 2021
    1. A word with multiple senses is likely to havemultiple, distinct tokens that can accurately be described as the direct hypernym for one or more ofits senses
    1. In coordpairs, a co-hyponym relationship is labelled 1 whereas other realtionships (direct hyponymy and direct hypernymy) are labelled 0.
    2. In entpairs, a direct hypernym relationship is labelled 1 whereas other relationships (hyponymy and co-hyponymy) are labelled 0.
  4. Feb 2021
    1. Nonviolent direct action seeks to create such a crisis and foster such a tension that a community which has constantly refused to negotiate is forced to confront the issue.

      How it works - what it seeks to accomplish.

    1. he most famous illustration of how the name of a substance is supposed to function in this way is provided not by Kripke, but by Putnam, another leading proponent of the ‘theory of direct reference’. Putnam asks us to imagine a Twin Earth – just like our Earth – which contains doppelgängers of us humans. The only difference between the two Earths is that on Twin Earth the clear, thirst-quenching, etc liquid that fills the oceans, lakes and rivers is not the chemical substance H2O, but another substance – XYZ. Suppose it’s 1750, before the chemical composition of water was discovered. On both Earths, the inhabitants call their liquid ‘water’. And, because it’s 1750, they associate the same mental checklist with that term: both think of ‘water’ as the substance that’s clear, thirst-quenching, boils at 100°C and so on. Now suppose a glass of XYZ is brought from Twin Earth to Earth and presented to Locke. Locke would believe it’s water, because it would tick his mental checklist. But would it be water? Not according to Putnam. Intuitively, that’s merely water-like stuff in the glass, not water. Putnam concludes that, while the term ‘water’ is associated with the same descriptions on Earth and Twin Earth, it has different meanings and picks out different chemical kinds. It is, and was, a necessary condition of something being water that it be H2O, despite this condition not being known back in 1750.
  5. Jan 2021
    1. Hi, I Need some help regarding my Ubuntu, is there any way to reach out to you personally ? Vote: 0 0 Share Facebook Twitter Copy link to comment Reply to SAK Copy link to comment Abhishek Prakash People's Favorite with 100+ Upvotes 30 Replies 3 weeks ago This comment is awaiting moderation Use our community forum, please.
    1. Indeed, the cell-lysate approach was found to be associated with superior sensitivity in some cases5.
    2. using a commercially available reagent (Bio-Rad SPR) that generates RT-qPCR-ready cell lysates with minimal manipulations. Similar reagents are now available from a number of sources (Ambion Cells-to-CT, Invitrogen CellsDirect, Roche RealTime Ready Cell Lysis, etc.)
    1. we demonstrate improved PCR-enhancing cocktails containing nonionic detergent, l-carnitine, d-(+)-trehalose, and heparin. These cocktails, in combination with two inhibitor-resistant Taq mutants, OmniTaq and Omni Klentaq, enabled efficient amplification of exogenous, endogenous, and high-GC content DNA targets directly from crude samples containing human plasma, serum, and whole blood without DNA purification.
    1. We found that a lysis solution containing both the non-ionic detergent (IGEPAL CA-630, chemically equivalent to Nonidet P-40 or NP-40) and bovine serum albumin (BSA) gave the best RT-qPCR yield. This direct lysis to reverse transcription protocol outperformed a column-based extraction method using a commercial kit.
  6. Oct 2020
  7. Sep 2020
    1. His skin was very sensitive, and he had to wear a big straw hat whenever he went out. W

      It's telling the readers directly that his skin is sensitive.

  8. Aug 2020
  9. Jun 2020
    1. Now all that was gone. In theory, they qualified for a $3,400 federal stimulus check, but they had no bank account or address to collect it. In theory, Dave was entitled to unemployment, but as of May only about 43 percent of the state’s 1.1 million claims had been paid.

      A few paragraphs later this appears: "She set her phone alarm for midnight so that as Thursday turned to Friday, she could check whether Dave’s first paycheck had hit their account. She could hear the clatter of raccoons foraging in a nearby dumpster and the hum of cars rolling through the drive-through. At 12:01 a.m. she looked to see whether Dave’s paycheck had shown up in his account." First they don't have an account for the stimulus money to go to, then they're getting up at midnight to see if the paycheck had hit their account. In addition, it takes about a week to set up direct deposit with most companies.

  10. May 2020
    1. But a subtle call-to-action gives people an excuse not to do as you ask. Starting a call-to-action with if, is the best way to give people an excuse. Be bossy and tell people exactly what you expect them to do.
  11. Apr 2020
    1. The tyranny of the majority (or tyranny of the masses) is an inherent weakness to majority rule in which the majority of an electorate pursues exclusively its own interests at the expense of those in the minority. This results in oppression of minority groups comparable to that of a tyrant or despot
    1. Direct democracy was not what the framers of the United States Constitution envisioned for the nation. They saw a danger in tyranny of the majority. As a result, they advocated a representative democracy in the form of a constitutional republic over a direct democracy. For example, James Madison, in Federalist No. 10, advocates a constitutional republic over direct democracy precisely to protect the individual from the will of the majority
    2. In the New England region of the United States, towns in states such as Vermont decide local affairs through the direct democratic process of the town meeting.[22] This is the oldest form of direct democracy in the United States, and predates the founding of the country by at least a century.
    1. There are “literally zero hoops,” one user in 4chan’s /pol/ forum told another in 2015. “Just sign up for Kindle Direct Publishing and publish away. It’s shocking how simple it is, actually.” Even Breivik, at the start of the 1,500-page manifesto that accompanied his terrorist attacks, suggested that his followers use KDP’s paperback service, among others, to publicize his message.
    1. When Casper filed its S-1 in January, analysts, investors, and business nerds descended on the document like vultures. Not only was it a precarious moment to take a startup public, it was the first time anyone could actually access the raw numbers under the hood of a DTC. “The economics work better if Casper sent you a mattress for free, stuffed with $300,” jabbed NYU Stern marketing professor and tech doomsayer Scott Galloway. “This appears to be Casper’s business,” tweeted number-crunching Atlantic columnist Derek Thompson. “Buy mattress at $400. Sell at $1,000. Refund/return 20% of them. Keep $400, on avg. Then spend $290 of that on ads/marketing and $270 on admin (finance, HR, IT). Lose $160. Repeat.”

      Summary of Casper's business model

    1. remember to re-raise any errors if the show_detailed_exceptions flag (a more appropriate check than consider_all_requests_local) is true
    2. That is a more direct flag to check for whether it is supposed to render the development/diagnostic "detailed" exceptions error page.
  12. Mar 2020
  13. Jul 2019
  14. Apr 2019
    1. using a commercially-available buffer that also requires a 15-minute incubation period prior to direct PCR
  15. Jan 2019
    1. In the field of comfort cooling, Indirect Evaporative Cooling (IEC) is an innovative and revolutionary change. The question is how eco-friendly are they? Evaporative cooling systems are upgraded by regular research and development for them to remain eco-friendly. Being cost effective and reliable is another important requirement to meet the varied industry demands.

  16. Nov 2018
    1. Use of Slack in a FACE-TO-FACE class and how much it increased interaction; brings up a point that concerns me and that's what happens when the instructor/TA appear to be available 24/7 given the nature of Slack; good exploration of motivating students to use it (4/5)

  17. Dec 2017
  18. Feb 2016
  19. Oct 2015