Listed on Hub.biz

Listed on Hub.biz

Listed on Tuugo.biz

Helping earners appropriately articulate their own skills is an equity issue.

Author response

To: Reviewer: Heikki Vapaatalo, MD, PhD, Emeritus professor of Pharmacolog

Dear reviewer Thank you very much for the insightful suggestions, the manuscript improved a lot with the changes performed. Please find the point-by-point answer to the raised questions. In the main text, all changes are highlighted in yellow. I hope that with the changes made the new version is suitable for publication.

Best regards Valquiria Bueno

General assessment

The study is interesting and the title promises for me more than the MS finally contains.

Answer: The manuscript is part of a project aiming to study ACE1 and ACE2 expression in cells from the immune system of aging and young adults. These initial results suggest that ACE1 (and probably ACE2) plays somehow a role in the process of aging.

The background, question and the aim are relevant as explained in the introduction.

Answer: We included a piece of information in the “Introduction” trying to link ACE1 expression in tissue cells and age-related diseases, as it follows:

ACE1 has been suggested to influence age-related diseases (i.e. Alzheimer’s, sarcopenia, cancer) but the associated mechanisms are still under investigation. ACE1 polymorphisms were correlated with susceptibility to Alzheimer’s disease (AD). [15, 16] In addition, it was shown recently that in normal aging ACE1 expression is increased in brain homogenates and this expression is unchanged in early stages of AD. [17] Regarding sarcopenia, Yoshihara et al. [18] found a weak correlation between ACE polymorphism and physical function. In cancer (gastric or colorectal), patients presented higher expression of ECA1 in tumor when compared with healthy tissues. [19, 20]

The major criticism concerns the small size of the material (subjects, n=6), the small age difference (64-67 years) and the lack of younger controls.

Answer: We agree that the small number of studied subjects is a limitation of this study. In spite of the interesting results suggesting that ACE1 expression could be linked to the health status, it was not possible to perform correlation analysis due to the small sample size. Even though there is a small chronological difference between the subjects, the biological aging is very different among them and reflects the genetics, lifestyle, nutrition and comorbidities. Another limitation is the lack of younger controls to compare with the subjects studied. Our next steps are to include younger controls, to increase the number of studied subjects, and if possible to get samples from older subjects (i.e. 70-80, 80 and more years old)

Minor notes:

1)Title: Angiotensin converting enzyme (ACE) expression in leukocytes of older adults

Answer: We evaluated only ACE1 expression, and thus, title, abstract, and main text were changed to ACE1 instead of ACE. We decided to change to title for: Angiotensin converting enzyme (ACE) 1 expression in leukocytes of adults from 64 to 67 years old

2)Introduction: The last chapter, the Author should explain in more detail, how references 11-14 suggest that “ACE play an important role in the aging process”. Does this mean, that ACE is somehow regulating the aging process or in increasing age ACE -levels are changed?

Answer: References 11-14 shows that age-related diseases occurring in older adults are associated with changes in the immune system. To complete the text we added:

ACE1 has been suggested to influence age-related diseases (i.e. Alzheimer’s, sarcopenia, cancer) but the associated mechanisms are still under investigation. ACE1 polymorphisms were correlated with susceptibility to Alzheimer’s disease (AD). [15, 16] In addition, it was shown recently that in normal aging ACE1 expression is increased in brain homogenates and this expression is unchanged in early stages of AD. [17] Regarding sarcopenia, Yoshihara et al. [18] found a weak correlation between ACE polymorphism and physical function. In cancer (gastric or colorectal), patients presented higher expression of ECA1 in tumor when compared with healthy tissues. [19, 20]

Material and Methods:

The N-value of the subjects should be mentioned here, as well the relation of females/males.

Answer: Text was correct as suggested Blood was collected from adults (n=6, four females and two males) aged 64-67 years old in 2015.

Do the Authors really regard 64-67 “older age” nowadays?

Answer: Nowadays the most common term used for individuals older than 65 years is “older adults”.

Why first many years later the assays have been done in comparison to the collection of the blood? Are the samples still useable, not destroyed?

Answer: Samples are part of UNIFESP Biobank and have been kept in adequate conditions. We wanted to test cells from a period anterior to COVID-19 and those samples were the only ones that attended our purpose. We compared samples used in this study with fresh blood samples (cell viability and percentage of CD4+, CD8+ and CD19+) and the results showed good preservation of the cells.

Did the subjects have some diseases and/or drugs because the possibly were from hospital sample bank?

Answer: Samples are part of UNIFESP Biobank, but unfortunately we do not have information about diseases and medicaments.

Express the company details similarly than Amersham, cities and countries.

Answer: Changes were done as required ACE CD143 FITC (R&D Systems, Inc, Minneapolis, USA)

Results:

“Table 1 shows that older adults…..” The comparison between the present data and historical studies belongs to the Discussion.

Answer: Changes were done as required

Give also individual ages and gender of the subjects in the table 1.

Answer: The manuscript version sent to medrxiv@medrxiv.org had age and gender on tables, but due to their request, any possible variable that could identify the studied individual had to be removed. That is why in the present version these variables are not shown.

What means p-values here? Compared with which or interindividual differences in the particular variable? Should be explained

Answer: We used p-value for interindividual differences in each variable since individuals age differently (biological aging) and thus, physiological parameters could be affected by genetics, lifestyle, nutrition, and comorbidities. It is now explained in materials and methods

The numbering of tables and the text seems to me confusing. Only three tables, but in the text mentioned four. Number 4 does not exist.

Answer: For some reason table 2 is missing in the main text, please find the new version with Table 2 included

It would be good to have a list of abbreviations used in the description of the cell types for an unfamiliar reader.

Answer: In each figure and table we are now providing a description of cells evaluated.

Discussion:

A major part of the discussion deals with previous publications and not meaning or clinical significance of the present findings and comparison between the present and earlier studies.

Answer: The discussion was changed as suggested:

Our results show that for the studied population, chronological aging and biological aging don´t go at the same pace. Even individuals having a small chronological difference (64 to 67 years old), they are heterogeneous for physiological parameters such as glucose, urea, glycated hemoglobin (Hbglic), and C-reactive protein (CRP). Changes in the same functional parameters have been reported by Carlsson et al. [22] and Helmerson-Karlqvist [23] in healthy older adults. Carlsson’s study [22] found that CRP value was 2.6 with a coefficient variation of 1.4% whereas in our study, it was observed higher values of CRP in 5 out of 6 individuals. Increased CRP levels has been associated with inflammaging and our findings show that the studied population has changes in functional parameters which are likely associated with an inflammatory profile. [24] The link between RAS and inflammation has been suggested but its role is not completely clear under physiological and pathological conditions. [25, 26] In addition, the association between ACE1 altered expression in tissues (brain, muscle, heart and vessels) and the development and progression of age-related conditions such as Alzheimer’s, sarcopenia, and cardiovascular disease has been suggested but results are controversial. [17, 27, 28, 29, 30] There are few studies showing the association between ACE1 expression in cells from the immune system (monocytes, T cells) and the progression of kidney and cardiovascular disease. [9, 8, 31, 32]. Therefore, considering the lack of information on this issue, we questioned whether ACE1 (CD143) was highly expressed in cells from the immune system during the aging process. We found that ACE1 was expressed in almost 100% of T (CD4+, CD8+) and B lymphocytes and in all phenotypes of these cells. In non-lymphoid cells, ACE1 mean expression was 56,9%. In agreement with our findings, independent studies showed that T cells from healthy donors and monocytes from patients with congestive heart failure expressed ACE1, but there was no investigation on cell phenotype. [25, 26]. Our study is the first to show that either inexperienced (naive) or fully activated (memory) cells expresses ACE1. Our findings suggest a that the expression of ACE1 in lymphoid and non-lymphoid cells reflects the health status since our studied population presented changes in physiological parameters and high levels of ACE1 expression on immune cells. Previous independent studies showed that patients with unstable angina [32] or acute myocardial infarction [33] presented higher expression of ACE1 in T cells and dendritic cells than controls subjects. In addition, markers of the cell (lymphoid and non-lymphoid) functional status such as inflammatory or growth factors production could be modulated by ACE inhibitors (ACEi). Accordingly, mononuclear leukocytes from healthy subjects incubated with endotoxin exhibited high levels of tissue factor activity which was reduced in the presence of captopril in a dose-dependent pattern. This result could be related to the antithrombotic effect of ACEi. [34]. In patients with congestive heart failure, immune cells cultured with LPS secreted high levels of the pro-inflammatory TNF-alpha and these levels were significantly reduced in the presence of captopril. [35]

In those previous studies, also ACE2 has been reported, why not studied here?

Answer: Our next studies will be focused on ACE1 and ACE2 expression in cells from the immune system in both younger and older adults.

In the limitations, the Authors fairly mention the real problem: The small sample size, and I would like to say lack of younger subjects.

Answer: we agree with the limitations pointed and the text was changed as required:

This study have limitations such as the small sample size and the lack of young adults for comparison. As an example, the subject with the highest CRP and albumin also exhibited a high percentage of ACE1 expression on T (CD4+, CD8+), B and non-lymphoid cells in addition to the lowest percentage of CD4+ naive cells, and the highest percentage of CD8+ terminally differentiated (EMRA) and DN B cells. However, due to the small sample size it was not possible to associate the high expression of ACE1 on immune cells with inflammaging and immunosenescence. It would bring important information to correlate physiological parameters/health status with ACE1 expression and to find out whether age and associated chronic diseases could lead to increased ACE1 expression.

The COVID-19 point even tempting today, is too far from this study and unnecessary. Answer: Our point was to emphasize the negative impact of chronic diseases for the outcome of aging population during a viral infection and how ACE1/ACE2 expression could bring information to diagnosis and treatment. Therefore, we would like to maintain this piece of information.

Linguistic checking would improve the MS. Answer: We checked for possible linguistic mistakes

Reviewer, Heikki Vapaatalo:

I read with pleasure the very detailed answers to my comments.

I very warmly recommend acceptance of this MS for publications without any further notes.

Decision changed:

Verified manuscript: The content is scientifically sound, only minor amendments (if any) are suggested.

To: Reviewer: Calogero Caruso

Dear Prof.Caruso Thank you very much for the revision of this manuscript. It is a privilege to have a manuscript reviewed by a research with high expertise on the field of ageing. Please find the answers to your questions and in the main text the changes in bold.

Sincerely yours,

Valquiria Bueno

The paper is essentially anecdotal because it studies the cells of 6 subjects without any comparison with other age groups. There is also a serious limitation because beyond the age and sex there is no information on the donors (how and why they were recruited, what drugs they took, etc.).

It is really a limitation to have only 6 individuals for the study, but they were the only ones fitting in the proposal of the manuscript. The samples were from a central bank of cells at UNIFESP and participants were considered “healthy” but there was not further information in addition to what we displayed on the tables of the manuscript. They were not living on homecares or hospitalized.

Our aim was to evaluate samples from individuals aged 60-69 years previously to COVID-19 and/or vaccination. In addition, there were no samples in the same conditions (PBMCs, -80oC) of young individuals and using fresh blood could bring a result that could not be compared mainly regarding to myeloid cells and B cells as is follows in the below reference. Braudeau C, Salabert-Le Guen N, Chevreuil J, Rimbert M, Martin JC, Josien R. An easy and reliable whole blood freezing method for flow cytometry immuno-phenotyping and functional analyses. Cytometry B Clin Cytom 2021;100(6):652-665. doi: 10.1002/cyto.b.21994.

Our goal from now on is to expand this study with young and old adults samples since it is important to understand whether ageing is associated with an increase in ACE expression on immune cells.

-To infer that chronological and biological ages do not match is inappropriate in the absence of the above information.

This piece of information regarding chronological and biological age was required by another reviewer. I agree that the concept does not match without more information on the donors. However, the information is now referenced and should be considered when older adults are studied. Vasto S, Scapagnini G, Bulati M, Candore G, Castiglia L, Colonna-Romano G, Lio D, Nuzzo D, Pellicano M, Rizzo C, Ferrara N, Caruso C. Biomarkes of aging. Front Biosci (Schol Ed) 2010;2(2):392-402. doi: 10.2741/s72. PMID: 20036955.

-However, the paper is of some interest because there are few studies on the topic.

Thanks for this positive comment. Few studies on the topic was the reason why we decided to send the manuscript for publication even though there were some important information on the donors missing and limited number of individuals.

Essential revisions that are required to verify the manuscript

1) Although we do not have data on donors, placing an age and gender column in all tables adds a minimum of useful information for the reader.

The first table submitted with age, but for requirement of MedRxiv, gender and age could no be linked to the metabolic results to preserve the anonymity of the donors.

2) Inflamm-ageing means low grade of inflammation. The value of CRP 23.1 suggests acute inflammation (also because albumin has high values, while in chronic inflammation its values decrease). Therefore the Ly averages do not have to take this subject into account.

Thank you for this comment. In a review of literature it was found an article (below) with CRP variation from 0.1 to 19.8 (Heumann Z, Youssim I, Kizony R, Friedlander Y, Shochat T, Weiss R, Hochner H, Agmon M. The Relationships of Fibrinogen and C-Reactive Protein With Gait Performance: A 20-Year Longitudinal Study. Front Aging Neurosci 2022;14:761948. doi: 10.3389/fnagi.2022.761948). There is also an article from your group showing CRPs <5g/dL and >5g/dL (Cancemi P, Aiello A, Accardi G, Caldarella R, Candore G, Caruso C, Ciaccio M, Cristaldi L, Di Gaudio F, Siino V, Vasto S. The Role of Matrix Metalloproteinases (MMP-2 and MMP-9) in Ageing and Longevity: Focus on Sicilian Long-Living Individuals (LLIs). Mediators Inflamm 2020;2020:8635158. doi: 10.1155/2020/8635158) that will be used to discuss how ageing impacts CRP levels. Considering the already small number of donors, data were maintained and statistics (mean + SD) with and without 23.1 mg/dL are now shown.

This will be the new version (discussion) about CRP Carlsson’s study [22] found that CRP value was 2.6 with a coefficient variation of 1.4% whereas in our study, it was observed higher values of CRP in 5 out of 6 individuals. In addition, it was shown by Cancemi et al. in an evaluation of individuals from 40 years to older than 95 years (long-living) that CRP increases in an age-dependent manner. Increased CRP levels has been associated with low grade of chronic inflammation (inflammaging) and our findings show that the studied population has changes in functional parameters which are likely associated with an inflammatory profile. [24] However, an individual presented CPR 23.1 mg/dL suggesting acute inflammation instead, but as all donors were not hospitalized or living on homecares, this sample was considered as part of the study. Another study evaluating gait speed found CRPs varying from 0.1 to 19.8mg/dL (Front Aging Neurosci 2022;14:761948.). Our study has an important limitation that is the lack of data on donors such as the use of continuous medicaments or sarcopenia, hypertension, cognition, among others, and thus it was not possible to correlate CRP with age-related conditions.

Table 1. Updated

Other suggestions to improve the manuscript The authors write that their findings suggest that ACE1 could play a role in several processes linked to aging including the generation and activation of autoimmune cells, due to the experimental evidence that inhibitors of ACE suppress the autoimmune process in a number of autoimmune diseases such as EAE, arthritis, autoimmune myocarditis. [49] They do not appear to have these findings in their paper. So, it needs to change the sentence.

Sentence changed to: According to experimental evidence, ACE inhibitors suppress the autoimmune process in a number of autoimmune diseases such as EAE, arthritis, autoimmune myocarditis. [49] Extrapolating these findings to our results, it is possible to suggest that ACE1 play a role in several processes linked to aging including the generation and activation of autoimmune cells.

Rviewer: Calogero Caruso

Decision changed:

Verified manuscript: The content is scientifically sound, only minor amendments (if any) are suggested.

A good summary of James Carse's book "Finite and Infinite Games"

https://shop.dias.ie/product-category/books/welsh/mediaeval-and-modern-welsh-series/

Overexpression of RECS1 in CQ-stressed cells causes LMP and translocation of BAX to the lysosome, which induces cell death. BAX is a proapoptotic protein.

Another example of someone saying that if their house were to catch fire, they'd save their commonplace book (first or foremost).

container images: whether to use full init process: implied here: don't need to if only using for single process (which doesn't fork, etc.)

HLTH Code on ProvenExpert

https://mycompanies.fandom.com/wiki/Yawman_%26_Erbe_Manufacturing_Company

Some fascinating advertisements for Yawman and Erbe

Yawman and Erbe Mfg. Co., - Criminal Identification - 1913<br /> https://scalar.chapman.edu/scalar/ah-331-history-of-photography-spring-2021-compendium/media/yawman-and-erbe-mfg-co---criminal-identification---1913

https://agincourtiowa.com/2015/06/02/yawman-erbe/

https://americanhistory.si.edu/collections/search/object/SILNMAHTL_34484

https://www.ebay.com/itm/125591176967?mkevt=1&mkpid=0&emsid=e11021.m43.l1120&mkcid=7&ch=osgood&euid=47f16005ebf04e3ca255c215366062fe&bu=45618614899&ut=RU&exe=0&ext=0&osub=-1%7E1&crd=20221102035021&segname=11021

https://www.newmarkettoday.ca/remember-this/in-its-heyday-office-specialty-employed-400-in-massive-complex-18-photos-1851941

you may carry on the inquiry for a lifetime without discovering other events inconsistent with the hypothesis which has caught your eye. But you can never get beyond a circumstantial case. . . .<br /> "A Footnote to the Safety-Valve," August 15, 1940, Paxson Papers (University of California Library, Berkeley)

One cannot excerpt their way into knowledge, simply cutting and pasting one's way through life is useless. Your notes may temporarily serve you, but unless you apply judgement and reason to them to create something new, they will remain a scrapheap for future generations who will gain no wisdom or use from your efforts.

relate to: notes about notes being only useful to their creator

Frederic Paxson took his note file from Wisconsin to Washington D.C. when he went into war service from 1917-1918, which Earl Pomeroy notes as an indicator of how little burden it was, but he doesn't make any notation about worries about loss or damage during travel, which may have potentially occurred to Paxson, given his practice and the value to him of the collection.

May be worth looking deeper into to see if he had such worries.

Do Catalysts control the direction of a reaction?

Why is it actually favorable for a cell to not be constantly energetically favorable to create a chemical reaction? Why does catalysts help control metabolic flux?

Relying on cut up slips of paper rather than the standard cards of equal size, Keith Thomas has relayed that his slips often "get loose and blow around the house, turning up months later under a carpet or cushion."

He also suspects that some of his notes have accidentally been thrown away by falling off his desk and into the nearby waste basket which camouflages his notes amongst similar looking trash.

episcopal-orthodox reply to: https://www.reddit.com/r/antinet/comments/y77414/comment/isyqc7b/

As long as you're not using flimsy, standard paper for your slips like Luhmann (they deteriorate too rapidly with repeated use), you can frame your carrying them around more positively by thinking that use over time creates a lovely patina to your words and ideas. The value of this far outweighs the fear of loss, at least for me. And if you're still concerned, there's always the option that you could use ars memoria to memorize all of your cards and meditate on them combinatorially using Llullan wheels the way Raymond Llull originally did. 🛞🗃️🚀🤩

u/LBHO https://www.reddit.com/r/antinet/comments/y77414/worried_about_paper_cards_being_lost_or_destroyed/

As a firm believer in the programming principle of DRY (Don't Repeat Yourself), I can appreciate the desire not to do the work twice.

Note card loss and destruction is definitely a thing folks have worried about. The easiest thing may be to spend a minute or two every day and make quick photo back ups of your cards as you make them. Then if things are lost, you'll have a back up from which you can likely find OCR (optical character recognition) software to pull your notes from to recreate them if necessary. I've outlined some details I've used in the past. Incidentally, opening a photo in Google Docs will automatically do a pretty reasonable OCR on it.

I know some have written about bringing old notes into their (new) zettelkasten practice, and the general advice here has been to only pull in new things as needed or as heavily interested to ease the cognitive load of thinking you need to do everything at once. If you did lose everything and had to restore from back up, I suspect this would probably be the best advice for proceeding as well.

Historically many have worried about loss, but the only actual example of loss I've run across is that of Hans Blumenberg whose zettelkasten from the early 1940s was lost during the war, but he continued apace in another dating from 1947 accumulating over 30,000 cards at the rate of about 1.5 per day over 50 some odd years.

Excusing criminality in a matter of deference to foreign nationals who are unlawfully present in the United States is perverse. The immigration laws have many provisions by design to ensure that foreign nationals who violate the laws of the United States in certain ways are not allowed to remain and harm the safety of Americans.

Crime has a significant effect on the entire New York City community, but especially on the low income community. Many NYC officials prioritize minimizing the effect of the law on criminals over minimizing the effect of criminals on law-abiding citizens.

DA Vance ignoring the possibility that people who engage in theft of public services are more likely to present a risk to public safety than those who do not.

DA Alvin Bragg continuing Cyrus Vance's policy of declining to enforce theft of public services law.

Violent incidents wherein officers are attacked trying to issue summonses to criminals engaging in turnstile jumping.

Increase in summonses for fare evasion in 2022 over 2021.

2020 statistics on cost of turnstile jumping.

Unclear why Washington DC, which is one of the highest crime jurisdictions in the United States and has serious financial issues, is a model to follow.

There's an unexplained assumption that people NYC-wide crime statistics should mirror population statistics. This is not the case with many crimes where enforcement disparities would have no effect, homicides being one example.

Former Governor Cuomo, who went on to sign the bail reform law, refusing to support Mayor de Blasio on the importance of policing fare evasion in 2018.

Former NYPD Commissioner Bill Bratton on the significance of policing fare evasion to New York City's revival in the 1990s.

Former Mayor de Blasio making a terrific point about the importance of policing fare evasion, an issue he understood despite not always acting in accordance with his correct statements.

@1:10:20

With HTML you have, broadly speaking, an experience and you have content and CSS and a browser and a server and it all comes together at a particular moment in time, and the end user sitting at a desktop or holding their phone they get to see something. That includes dynamic content, or an ad was served, or whatever it is—it's an experience. PDF on the otherhand is a record. It persists, and I can share it with you. I can deliver it to you [...]

NB: I agree with the distinction being made here, but I disagree that the former description is inherent to HTML. It's not inherent to anything, really, so much as it is emergent—the result of people acting as if they're dealing in live systems when they shouldn't.

Writer Jean Paul on the importance of his Zettelkasten.

"In the event of a fire, the black-bound excerpts are to be saved first," the poet Jean Paul instructed his wife before setting out on a journey in 1812.

link to: https://hyp.is/BLL9TvZ9EeuSIrsiWKCB9w/ryanholiday.net/the-notecard-system-the-key-for-remembering-organizing-and-using-everything-you-read/

https://www.reddit.com/r/antinet/comments/ur5xjv/handwritten_cards_to_a_digital_back_up_workflow/

For those who keep a physical pen and paper system who either want to be a bit on the hybrid side, or just have a digital backup "just in case", I thought I'd share a workflow that I outlined back in December that works reasonably well. (Backups or emergency plans for one's notes are important as evidenced by poet Jean Paul's admonition to his wife before setting off on a trip in 1812: "In the event of a fire, the black-bound excerpts are to be saved first.") It's framed as posting to a website/digital garden, but it works just as well for many of the digital text platforms one might have or consider. For those on other platforms (like iOS) there are some useful suggestions in the comments section. Handwriting My Website (or Zettelkasten) with a Digital Amanuensis

His collection is incredibly important to him. He states this in a way that's highly reminiscent of Jean Paul.

"In the event of a fire, the black-bound excerpts are to be saved first." —instructions from Jean Paul to his wife before setting off on a trip in 1812 #

18 Von Bülow and Krusche, “Vorla ̈ ufiges,” 273.

Hans Blumenberg's first zettelkasten dates to the early 1940s, but was lost during the war though he continued the practice afterwards. The collection of his notes housed at Marbach dates from 1947 onward.

Hans Blumenberg's zettelkasten of about thirty thousand typed and handwritten note cards is now kept at the German Literature Archive in Marbach. Blumenberg kept it in a wooden box which he kept in a fireproof steel cabinet.

If a foreign national who is in the United States without legal authorization does something stupid and is required to appear in Court as a result, he or she may be more likely to come to the attention of immigration authorities. As an initial matter, the solution is to not violate the immigration laws of the United States. However, if one chooses to violate the immigration laws, he or she ought to avoid doing things like jumping turnstyles. Many Americans likely avoid taking certain liberties that they do in the United States when they are traveling in foreign countries.

It is unclear to me how the MTA's own incompetence exonerates people from stealing public services. I am confident that fare beaters, black, brown, white, or anything else, are stealing public services because the MTA is a train-wreck. Both issues contribute to the current mess in the NYC Subway system, but they are not otherwise related.

Not prosecuting crimes is a positive trend, apparently. This disregards how NYC transformed itself in the 90s and 00s under the leadership of Mayors Giuliani and Bloomberg, and how that success was maintained at least when former Mayor de Blasio wisely chose William Bratton as NYPD Commissioner.

This must be it. There are no alternative explanations such as the possibility that certain crimes may be disproportionately committed by people who share one characteristic and not another (see NYC homicide statistics). Moreover, it is unclear to me why the writer is lumping "black and Hispanic" people together since, if this is purely a race-based claim against the NYPD, there may be different statistics for these two very broad groups.

This disregards the fact that there is a high correlation between "behaviors that cause the most harm" and "fare evasion," lest the author would suggest that of people who commit crimes on transit, a meaningful number of them pay the fare.

I will venture that most New Yorkers are more concerned about lawless behavior on subways than by the presence of uniformed police.

Mayor Adams was correct to the extent that he noted that turnstyle jumping is a crime and should not be permitted - however, he has not used all of tools at his disposal to police the Subways against the opposition of the District Attorneys.

This is a very important point. Policing fare evasion is not only a financial issue or a fairness question, it is a matter of public safety. It is true that not every person who engages in theft of public services is violent, but as Andrew Albert notes, violent felons are overwhelmingly likely to not pay MTA fares.

This is a very interesting passage. It highlights that the NYPD is free to enforce the law and make arrests notwithstanding the efforts of District Attorneys to rewrite the law through the refusal to prosecute laws that they do not like. Former DA Vance's "displeasure" highlights that the NYPD and Mayor are not helpless - and that they can put pressure on lawless District Attorneys by continuing to enforce the law. The refusal of the Mayor and the NYPD leadership to use the tools in their toolbox has been a driving force in the increase in fare-beating.

Well said.

Theft of public services, like other kinds of theft, does have the potential to "burden" offenders with a criminal record.

sense of humanity. one human race. important reason to how land loss took place. Natives understanding of property.

Kriminologer påpekar att forskning visar att visitationszoner inte gör någon egentlig skillnad. Dessutom utsätts oskyldiga för trakasserier.

Stop-and-search zones in Sweden mane no difference and cause innocent people to feel harassed.

Popular Diet Trends for Weight Loss

HLTH Code Profile on 500px

Slashdot.org Profile - HLTH Code

⬑ "The Code Rot Problem"

Jay talks about the depth of the second cognitive illusion, thematized in Buddhism but not so much in Western philosophy - the illusion of a self with respect to other.

4E (Embedded, Embodied, Enactive, Extended) Cognition is based on an intuitive idea that we know from very simple experience - you and I are part of the world. We have bodies that are embedded in reality.

We have a reflexive and profoundly entrenched embrace of dualism - that we are NOT of this world, but stand apart from it. This cognitive illusion is EXTREMELY hard to penetrate.

HLTH Code on Bored Panda

Again he uses the idea of a "file" which I read and understand as similar to the concepts of zettelkasten or commonplace book. Unlike others writing about these concepts though, he seems to be taking a more holistic and integrative (life) approach to having and maintaining such system.

Perhaps a more extreme statement of this might be written as "zettelkasten is life" or the even more extreme "life is zettelkasten"?

Is his grounding in sociology responsible for framing it as a "social organization" of one's memory?

It's not explicit, but this statement could be used as underpinning or informing the idea of using a card index as autobiography.

How does this compare to other examples of this as a function?

Current revision of README: https://github.com/t-mart/kill-sticky/blob/124a31434fba1d083c9bede8977643b90ad6e75b/README.md

We're creating a bookmarklet, so our code needs to be minified and URL encoded.

Run the following the project root directory:

$ docker build . -t kill-sticky && docker run --rm -it -v $(pwd):/kill-sticky kill-sticky

https://www.youtube.com/watch?v=wRssjvU2d-s

Starts out with some of the personal histories of how both got into the note making space.

This got more interesting for me around the 1:30 hour mark, but I was waiting for the material that would have shown up at the 3 hour mark (which doesn't exist...).

Scott spoke about the myths of zettelkasten. See https://www.reddit.com/r/Zettelkasten/comments/urawkd/the_myths_of_zettelkasten/

He also mentions maintenance rehearsal versus elaborative rehearsal. These are both part of spaced repetition. The creation of one's own cards helps play into both forms.

Best Weight Loss Drinks - According to Nutritionists

There's an irony here in that this "cult" may have actually been a cult of teachers and students. Should the broader thesis bear out, we're going to have lots of references to these cults of teachers lingering in the literature....

This course is designed for absolute beginners who have a fundamental understanding of R and statistics. No worries, you will get it from the coursework if you do not have any prior knowledge. It starts with a basic understanding of data sets and processes and continues till advanced steps.

One of the underlying commonalities about the various myths of poverty is that we tend to "other" those that it effects. The "them" we stigmatize with the ills of poverty really look more like "us", and in fact, they are.

Rather than victim shame and blame those in poverty, we ought to spend more of our time fixing the underlying disease instead of spending the time, effort, energy, and money on attempting to remedy the symptoms (eg. excessive policing, et al.) Not only is it more beneficial, but cheaper in the long run.

Related:<br /> Gladwell, Malcolm. “Million-Dollar Murray.” The New Yorker, February 5, 2006. https://www.newyorker.com/magazine/2006/02/13/million-dollar-murray (.pdf copy available at https://housingmatterssc.org/million-dollar-murray/)

IS IT BECAUSE IT CHANGES WHERE WE PUT EMPHASIS

So... line breaks change the emphasis and myster of poetry of each line (ie state of heightened anxiety) but also how we literally pronounce them out loud (what pitch we use)

Tweed would overprice projects he promised to finish for poor immigrant taxpayers, just so he could pocket the money and gain power and control over the immigrants' votes and politics.

Nast, being Republican during this time, supported the freedom and equality of former slaves. Nast was also probably concerned with the failing economy and banks around the country, which would explain why he abhorred Tweed and his practices. Tweed was a Democrat seeking power; he did not agree with the equality of former slaves and white men, and because of urbanization and industrialization, he was most likely attaining power and money, profiting from the two.

Can copyright vest in an AI?

The primary objective of intellectual property law is to protect the rights of the creators of intellectual property.10 Copyright laws specifically aim to: (i) promote creativity and encourage authors, composers, artists and designers to create original works by affording them the exclusive right to exploit such work for monetary gain for a limited period; and (ii) protect the creators of the original works from unauthorised reproduction or exploitation of those works.

To my knowledge, conferring copyright in works generated by artificial intelligence has never been specifically prohibited. However, there are indications that the laws of many countries are not amenable to non-human copyright. In the United States, for example, the Copyright Office has declared that it will “register an original work of authorship, provided that the work was created by a human being.” This stance flows from case law (e.g. Feist Publications v Rural Telephone Service Company, Inc. 499 U.S. 340 (1991)) which specifies that copyright law only protects “the fruits of intellectual labor” that “are founded in the creative powers of the mind.” Similarly, in a recent Australian case (Acohs Pty Ltd v Ucorp Pty Ltd), a court declared that a work generated with the intervention of a computer could not be protected by copyright because it was not produced by a human.

With the advent of AI software, computers — not monkeys — will potentially create millions of original works that may then be protected by copyright, under current law, for more than 100 years.

What a watermeleon- I just made that up to say.

Why did I not know this before... So I used to literally call out my students for Snap-chatting in class.

If I knew about this educational use of Snap,, I would have used it already.

Could this be linked to my FUD thesis about decisions based on possibilities rather than realities?

I took along my son, who had never had any fresh water up his nose and who had seen lily pads only from train windows. On the journey over to the lake I began to wonder what it would be like. I wondered how time would have marred this unique, this holy spot--the coves and streams, the hills that the sun set behind, the camps and the paths behind the camps. I was sure that the tarred road would have found it out and I wondered in what other ways it would be desolated. It is strange how much you can remember about places like that once you allow your mind to return into the grooves which lead back. You remember one thing, and that suddenly reminds you of another thing. I guess I remembered clearest of all the early mornings, when the lake was cool and motionless, remembered how the bedroom smelled of the lumber it was made of and of the wet woods whose scent entered through the screen. The partitions in the camp were thin and did not extend clear to the top of the rooms, and as I was always the first up I would dress softly so as not to wake the others, and sneak out into the sweet outdoors and start out in the canoe, keeping close along the shore in the long shadows of the pines. I remembered being very careful never to rub my paddle against the gunwale for fear of disturbing the stillness of the cathedral.

visible-web-page looks to have been published and/or written on 2022 June 26.

I emailed Omar a few weeks earlier (on 2022 June 7) with with a link to plain.txt.htm, i.e., an assembler (for Wirth's RISC machine/.rsc object format) written as a text file that happens to also allow you to run it if you're viewing the text file in your browser.

(The context of the email was that I'd read an @rsnous tweet(?) that "stuff for humans should be the default context, and the highly constrained stuff parsed by the computer should be an exceptional mod within that", and I recognized this as the same principle that Raskin had espoused across two pieces in ACM Queue: The Woes of IDEs and Comments Are More Important Than Code. Spurred by Omar's comments on Twitter, I sent him a link to the latter article and plain.txt.htm, and then (the next day) the former article, since I'd forgotten to include it in the original email.)

prone to naming winners and losers, rights and wrongs. You are in or out, hot or not, on the bus or off it. But academics seldom write in an all-or- nothing mode” (p. 26).

Our culture is overly based on the framing of winners or losers and we don't leave any room for things which aren't a zero sum game. (See: Donald J. Trump's framing of his presidency.) We shouldn't approach academic writing or even schooling or pedagogy in general as a zero sum game. We need more space and variety for neurodiversity as teaching to the middle or even to the higher end is going to destroy the entire enterprise.

Politics is not a zero sum game. Even the losers have human rights and deserve the ability to live their lives.

!- in other words : show, instead of tell

!- for : futures - very appropriate description of what appears to be the most sensible futures for civilization

That is, the advantage of folk- lore is that it conveys what people think in their own words and actions, and what they

say or sing in folklore expresses what they might not be able to in everyday conversation.

Others, well understanding the advantages of systematic classification, have proposed to fit their materials, as fast as collected, into their appropriate places in a prearranged scheme. For this purpose they use notebooks of which every page has first been provided with a heading. Thus all the entries of the same kind are close to one another. This system leaves something to be desired; for addi

The use of a commonplace method for historical research is marked as a poor choice because:<br /> The topics with similar headings may be close together, but ideas may not ultimately fit into their pre-allotted spaces.<br /> The classification system may be too rigid as ideas change and get modified over time.

They mention that librarians used to catalog books in this method, but that they realized that their system would be out of date almost immediately. (I've got some notes on this particular idea to which this could be directly linked as evidence.)

https://lu.ma/w6c1b9cd

[[Anne-Laure Le Cunff & Nick Milo - How can we do Combinational Creativity]]

Details

Date: [[2022-09-06]]<br /> Time: 9:00 - 10:00 AM<br /> Host: [[Nick Milo]]<br /> Location / Platform: #Zoom<br /> URL: https://lu.ma/w6c1b9cd<br /> Calendar: link <br /> Parent event: [[LYT Conference 2]]<br /> Subject(s): [[combinational creativity]]

To Do / Follow up

• [ ] Clean up notes
• [ ] Post video link when available (@2022-09-11)

Video

TK

Attendees

Notes

generational effect

Silent muses which resulted in drugs, alcohol as chemical muses.

All creativity is combinational in nature. - A-L L C

mash-ups are a tacit form of combinatorial creativity

Methods: - chaining<br /> - clustering (what do things have in common? eg: Cities and living organisms have in common?)<br /> - c...

Peter Wohlleben is the author of “hidden life of trees”

CMAPT tools https://cmap.ihmc.us/

mind mapping

Metaphor theory is apparently a "thing" follow up on this to see what the work/research looks like

I put the following into the chat/Q&A:

The phrase combinatorial creativity seems to stem from this 2014 article: https://fs.blog/networked-knowledge-and-combinatorial-creativity/, the ideas go back much further obviously, often with different names across cultures. Matt Ridley describes it as "ideas have sex" https://www.ted.com/talks/matt_ridley_when_ideas_have_sex; Raymond Llull - Llullan combinatorial arts; Niklas Luhmann - linked zettels; Marshall Kirkpatrick - "triangle thinking" - Dan Pink - "symphonic thinking" are some others.

For those who really want to blow their minds on how not new some of these ideas are, try out Margo Neale and Lynne Kelly's book Songlines: The Power and Promise which describes songlines which were indigenous methods for memory (note taking for oral cultures) and created "combinatorial creativity" for peoples in modern day Australia going back 65,000 years.

Side benefit of this work:

"You'll be a lot more fun at dinner parties." -Anne-Laure

Improv's "yes and" concept is a means of forcing creativity.

Originality is undetected plagiarism - Gish? English writer 9:41 AM quote; source?

Me: "Play off of [that]" is a command to encourage combintorial creativity. In music one might say "riff off"...

Chat log

none available

This is so true ,because like in 2022 knowledge is mostly what rule this world not the president. One example , during covid the pople that were mostly listened to were the doctors, scientist and any health officials. The president gave a thumbs up for the plans to follow through but without the healthcare officials things could've gotten way worse than it was. one more example , I there is a shortage of clean water, chemist would be very needy because there is a way to purify dirty water, some companies are innovating on that matter, making it possible to drink a dirty water clean.

Ben Thompson argues that questions about Google's behavior towards a false positive case of CSAM does not pertain to free speech or to the First Amendment. But it does pertain to other Amendments in the Bill of Rights.

Timestamps 19:11 - 35:20

CA Senate Bill 488 2021; signed, in process,

Orton-Gillingham method (procedure/process) but can be implemented differently. Rigorous and works. Over 100 years old.

Wilson program uses pieces of OG. What's this? Not enough detail here.

Dyslexia training will be built into some parts of credentialling programs.

Each child is different.

This requires context knowledge on the part of the teacher and then a large tool bag of methods to help the widest variety of those differences.

In the box programs don't work because children are not one size fits all.

Magic wand ? What would you want?

Madhuri would like to have: - rigorous teaching in early grades - if we can teach structured literacy following a specific scope in sequence most simple to most complex - teaching with same familiar patterns over and over - cumulative (builds on itself) - multisensory - explicit - Strong transitional kindergarten through grade 3 instruction

Prevention trumps intervention.

Otherwise you're feeding into the school to prison pipeline.

Madhuri's call for teaching that is structured, cumulative, multisensory, and explicit sounds a lot like what I would imagine orality-based instruction looks like as well. The structure there particularly makes it easier to add pieces later on in a way that literacy doesn't necessarily.

both the ballad and the murder case were based on the trial of Pickett and Carey for the murder of Stevenson in Sibsey.

In his youth, while selling drugs on the side, Jay-Z would write down material for lyrics into a green notebook. He never took the notebook around with him on the streets, but instead would buy anything at a corner store just for the paper bags as writing material. He would write the words onto these paper bags and stuff them into his pockets (wearable Zettelkasten anyone? or maybe Zetteltasche?) When he got home, in long standing waste book tradition, he would transfer the words to his notebook.

Jay-Z has said he hasn't written down any lyrics since his first album, but warns, "I've lost plenty of material. It's not the best way. I wouldn't advise it to anyone. I've lost a couple albums' worth of great material."

https://ondemand.npr.org/anon.npr-mp3/npr/fa/2010/11/20101116_fa_01.mp3

Link to: https://hypothes.is/a/T3Z38uDUEeuFcPu2U_w_zA (Jonathan Edwards' zettelmantle)

Huang, P. (2021, April 1). How The CDC Is Battling The Pandemic And Working To Regain Public Trust: Shots—Health News: NPR. https://www.npr.org/sections/health-shots/2021/04/01/982761755/inside-the-cdcs-battle-to-defeat-the-virus?utm_campaign=storyshare&utm_source=twitter.com&utm_medium=social

“A lie will go round the world while truth is pulling its boots on.” C. H. Spurgeon

Mulot, M., Segalas, C., Leyrat, C., & Besançon, L. (2021). Vaccination rates and COVID-19 cases: A commentary of “Increases in COVID-19 are unrelated to levels of vaccination across 68 countries and 2947 counties in the United States.” OSF Preprints. https://doi.org/10.31219/osf.io/72abp

Ro, C. (2021, November 1). Why mandatory vaccination is nothing new. https://www.bbc.com/future/article/20211029-why-mandatory-vaccination-is-nothing-new

John Bye [@_johnbye]. (2021, October 6). The new covid sceptic All Party Parliamentary Group on Pandemic Response and Recovery is backed by Gupta and Heneghan’s Collateral Global to the tune of over £30,000. £5,000 in financial benefits plus £25,501—£27,000 benefits in kind (CG is acting as their secretariat). Https://t.co/qll20Sg9aA [Tweet]. Twitter. https://twitter.com/_johnbye/status/1445867760819396608

Anthony Costello. (2022, February 24). The risks of cognitive symptoms lasting at least 12 MONTHS were much higher in the infected group. 4.8x higher for fatigue, 3.2x for brain fog, 5.3x for poor memory, and an incredible 51x for altered taste and smell. We need data on children, but it could easily be similar. (17) https://t.co/JC1qYyW2Xc [Tweet]. @globalhlthtwit. https://twitter.com/globalhlthtwit/status/1496957266016313348

Atari, M., Reimer, N. K., Graham, J., Hoover, J., Kennedy, B., Davani, A. M., Karimi-Malekabadi, F., Birjandi, S., & Dehghani, M. (2021). Pathogens Are Linked to Human Moral Systems Across Time and Space. PsyArXiv. https://doi.org/10.31234/osf.io/tnyh9

Holford, D. L., Juanchich, M., & Sirota, M. (2021). Ambiguity and unintended inferences about risk messages for COVID - 19. PsyArXiv. https://doi.org/10.31234/osf.io/w5rd6

Jørgensen, F. J., Nielsen, L. H., & Petersen, M. B. (2021). Willingness to Take the Booster Vaccine in a Nationally Representative Sample of Danes. PsyArXiv. https://doi.org/10.31234/osf.io/wurz8