11 Matching Annotations
  1. Aug 2019
  2. May 2019
    1. SDS-PAGEwas performed as described previously (Laemilli, 1970).10-40 μg protein samples were mixed with 4X SDS loading buffer and either incubated at 50 ̊C or 90 ̊C for 10 min. Denatured samples were loaded either on8%or 10%SDS-PAGEgel and run in Tris-Glycine-SDSgel running buffer at 70-100 Volts for 2-3 hin a Mini-PROTEAN®3electrophoresis unit(Bio-Rad).After electrophoresis,gels were either visualized by coomassie brilliant blue (CBB) stainingor processedfor western blotting as described below
  3. Feb 2019
    1. the melancholy mournings of the turtle,

      gilmanhernandez already linked to the video I was considering, but (according to a cursory search of YouTube at least) videos of turtle sounds are also likely to be videos of turtles mating or attempting to mate, so how 'mournful' they are is perhaps up to interpretation... 😂

  4. Dec 2018
    1. How Mobile App Development Fuel the Success of E-commerce Business

      M-commerce is for the mobile e-commerce. Recent research by Business insider state that the M-commerce will increase by 45% of total e-commerce which equals $284 billion in sales by the end of 2020. So having the mobile app is crucial if you wish your brand to reach across different users. Today the Smartphones have changed the completely the way business used to work. Mobile apps have provided businesses with a more mobile mode of working. Now the smartphones can be smoothly incorporated into all types of businesses- be it small or large businesses, e-commerce or organization. Mobile apps help to improve the way customers communicate with businesses.

  5. Nov 2018
    1. Unfortunately you need to install the postgresql package, because Homebrew does not currently provide a standalone libpq package.

      Interestingly enough, I found that that "brew install libpq" installed from "https://ftp.postgresql.org/pub/source/v11.0/postgresql-11.0.tar.bz2 ... Summary 🍺 /usr/local/Cellar/libpq/11.0: 2,561 files, 27.2MB, built in 4 minutes 30 seconds."

      Mac Pro on Mac OX 10.11.6.

  6. Aug 2018
  7. edu522.networkedlearningcollaborative.com edu522.networkedlearningcollaborative.com
    1. Incessentaly correcting graamer

      This may be my favorite line of the entire code of conduct! I'm doing my best to resist....

  8. Nov 2017
    1. When you think the problem to be solved is the high cost of textbooks, inclusive access programs and OER adoption are just two competing approaches to solving the problem.

      There was an interesting example of this during a short conference on digital textbooks, back in late 2014. Cindy Ives interim VP Academic at Athabasca (!) presented the etext pilot project in partnership with publishers. Ives’s approach was quite pragmatic and there’s nothing wrong with doing a pilot project on something like this. By that time, Ives was already involved in OER projects. It still struck a chord with those of us who care about OER, including Éric Francoeur who took an active part in the event and did work to create a free textbook through international and interlinguistic collaboration.

      To me, a key notion from the ‘r’ in “OER” is the distinction with those content bundles we still call “textbooks”. Sure, it’s already in the 5-R model. But the “Remix” idea in music is to a large extent about unbundling.

  9. Oct 2017
  10. Apr 2017
    1. Episode 077: Tapping Into Abundance

      Recent Episodes Can we only have 4 episodes show up here so the page isn't so long? M

  11. Apr 2016
    1. Encourage researchers not to transfer the copyright on their research outputs before publication.

      This statement is more generally applicable than just to TDM. Besides, "Encourage" is too weak a word here, and from a societal perspective, it would be far better if researchers were to retain their copyright (where it applies), but make their copyrightable works available under open licenses that allow publishers to publish the works, and others to use and reuse it.

  12. Nov 2015
    1. If the space doctor’s ideas were wrong, your phone wouldn’t be able to tell where it was.

      If all of the space boats travel at the same velocity and the same distance from the Earth's surface, the time shift of the on-board clock due to relativity would be the same. The difference between the clocks would be almost the same. How would the trouble appear?