- Oct 2024
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Limestone & Karst Terrain The Valley’s abundant limestone is evidence that the area was once under ocean water, long before the tectonic plates collided. Limestone forms in warm, shallow seas when generations of tiny sea creatures die and accumulate on the ocean floor, later to compress into solid rock. Karst Because of its limestone base, the Shenandoah Valley is karst, a terrain type with distinctive landforms and water resources. Slightly acidic water wore through the bedrock over many millions of years, leaving caves, caverns, and sinkholes. Streams and springs disappear and reappear over time. Aquifers Water that filters through the rocks collects in underground reservoirs, or aquifers. Aquifers are underground rock layers filled with groundwater in the spaces between fractured limestone, silt, gravel, and sand. Aquifers closer to the surface, used for irrigation and drinking water, are re-charged by rainwater. They are very useful but also extremely vulnerable to contamination. Caves & Caverns The water that dissolves limestone and other soluble rocks in the Shenandoah Valley leaves behind caves and caverns. In some caves and caverns, the deposits of dissolved minerals left by dripping water make the features of stalactites, stalagmites, and columns. Luray Caverns, a National Natural Landmark in the Valley, is ornately decorated with cascades, columns, stalactites, stalagmites and pools. Other Rocks & Minerals Many rocks and minerals occupy the Shenandoah Valley besides limestone. Other sedimentary rocks in the region are sandstone, shale, and coal. There are igneous rocks like granite, gabbro, and basalt, and metamorphic rocks like slate, gneiss, and quartzite. Manganese, iron ores, zinc, lead, sulfur, gypsum, and pyrite are relatively common Shenandoah Valley minerals. The Valley yields small amounts of oil and natural gas. Also present is red clay, an oceanic sediment with iron oxide, along with fossils of sea creatures like brachiopods and trilobites.
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- Sep 2024
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www.curbed.com www.curbed.com
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A book is a complete discrete object, cut to fit and shaped for engaging reading, but thousands upon thousands of loose pages in their archival boxes constitute something else: a relay baton handed off to the future.
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Local file Local file
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I've learned to do without a lot of things. If you have a lot of things, saidAunt Lydia, you get too attached to this material world and you forget aboutspiritual values. You must cultivate poverty of spirit. Blessed are the meek.
Which contradicts her desire to treat her body, to have skin cream, forcing her to use even butter. Both women are pitted against each other because they have one but not the other -- one has their fertility and the other has their vanity.
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- Aug 2024
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www.youtube.com www.youtube.com
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when the body dies you are gone because you are the body in this other theory on the other hand we are the field that controls the body so when the drone dies don't go anywhere you stay where you were and you continue to live
for - comparison of death in - material vs idealist theories
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github.com github.com
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This seems to be more than just a thin wrapper like https://github.com/rainerschuster/final-form-material-ui was. I kind of prefer the simplicity of focus of final-form-material-ui.
This appears to be attempting to do too much. Though if it gives you exactly what you want, great.
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- Jul 2024
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for - sustainable building - phase change material - PCM - DIY - wearable cooling vest - phase change material - PCM
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royaldanishacademy.com royaldanishacademy.com
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for - sustainable building - PCM - phase change material
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docdrop.org docdrop.org
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the idea took hold that and this was defined by the british economist uh sydney pollard in his book called the idea of progress and i'm just 00:02:54 paraphrasing here but essentially he said the uh the i the assumption is that there's a pattern of change in history and that 00:03:07 these consist of changes in one direction only and that that direction is towards improvement
for - definition - progress - material - economist Sydney Pollard - improvement
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- Apr 2024
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Local file Local file
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Inthe case of good books, the point is notto see how many of them you can getthrough, but rather how many can getthrough you—how many you can makeyour own
This is not only a nice quote by itself, but seems to be saying something deeper to me about productivity.
There's a difference in productivity for it's own sake, but being both productive in the send of time spent efficiently and productive in the sense of producing something of greater value with your time than you might have spent doing something else which was less valuable, but which might still have been time well spent.
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- Feb 2024
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www.derstandard.de www.derstandard.de
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Eine neue Studie der Universität für Bodenkultur beziffert erstmals, wieviel Kohlenstoff zwischen 1900 und 2015 langfristig oder kurzfristig in menschlichen Artefakten wie Gebäuden gespeichert wurde. Die Menge des dauerhaft gespeicherten Kohlenstoffs hat sich seit 1900 versechzehnfacht. Sie reicht aber bei weitem nicht aus, um die globale Erhitzung wirksam zu beeinflussen. Die Möglichkeiten, Boot in Gebäuden zu nutzen, um der Atmosphäre CO2 zu entziehen, werden bisher nicht genutzt. https://www.derstandard.at/story/3000000208522/co2-entnahme-durch-holzbau-ist-bisher-nicht-relevant-fuer-den-klimaschutz
Studie: https://iopscience.iop.org/article/10.1088/1748-9326/ad236b
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Local file Local file
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StAugustine draws this same parallel when he writes to a communityof nuns, ‘let it not be only your mouth that takes food, but let yourears also drink in the word of God’.3
quoted section from:
St Augustine, Letters, trans. by Wilfrid Parsons, 5 vols. (Baltimore, MD: Catholic University of America Press, 1956), V, Letter 211, ‘To a Convent of Consecrated Virgins’, p. 43.
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‘Blessed Lord, which hast caused al holy Scriptures to bee written forour learnyng; graunte us that we maye in such wise heare them,read, marke, learne, and inwardly digeste them.’2
quote from:<br /> The Booke of the Common Prayer and Administration of the Sacraments (London: 1549), sig. B iiv.
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- Jan 2024
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docdrop.org docdrop.org
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for - building - material - earth - Elisabetta Carnevale - BC Materials - Cycle Terre - Joseph Colzani - Center of the Earth
summary - A good outline of contemporary earth building techniques.
to - Cycle Terre - https://hyp.is/p2mdqLuTEe6W0XcNljBbWA/www.cycle-terre.eu/mise-en-oeuvre/les-materiaux/ - BC Material - https://hyp.is/BY5_nLuVEe6v4-dubnc59A/bcmaterials.org/ - BC Material Studies - https://hyp.is/BY5_nLuVEe6v4-dubnc59A/bcmaterials.org/ - Joseph Colzani and Center of the Earth - https://hyp.is/YvQIeLu4Ee6DACOMT1xOdw/www-centredeterre-fr.translate.goog/le-centre-de-terre/?_x_tr_sch=http&_x_tr_sl=fr&_x_tr_tl=en&_x_tr_hl=en&_x_tr_pto=sc
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in general countries tend to excavate enormous volumes of earth and this earth is incredibly considered as a waste material
for - circular economy - building - excavation waste - circular economy - construction - excavation waste - key insight - repurpose excavation waste as building material
key insight - She makes an pretty important observation about the inefficiency of current linear construction process - The excavation part requires enormous amounts of energy, and the earth that is excavated is treated as waste that must be disposed of AT A COST! - Instead, with a paradigm shift of earth as a valuable building resource, the excavation PRODUCES the building materials! - This is precisely what BC Material's circular economy business model is and it makes total sense!<br /> - With a simple paradigm and perspective shift, waste is suddenly transformed into a resource! - waste2resource - waste-to-resource
new meme - Waste-2-Resource
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helioterra
for - helioterra - building - material - earth - phase change
Tags
- BC Materials studies
- circular economy - building - excavation waste
- architect - Joseph Colzani - Center of the Earth
- building - material - earth - phase change
- new meme - waste-to-resource
- key insight - repurpose excavation waste as building material
- architect - Elisabetta Carnevale
- circular economy construction - excavation waste
- waste-2-resource
- waste-to-resource
- Cycle Terre
- building - material - earth
- new meme - waste2resource
- Helioterra
- BC Materials
Annotators
URL
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- Dec 2023
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Local file Local file
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“I do all my own research,” she said, “though reviewers have speculatedthat I must have a band of hirelings. I like to be led by a footnote ontosomething I never thought of. I rarely photocopy research materials because, for me, note-taking is learning, distilling. That’s the whole essence ofthe business. In taking notes, you have to discard what you don’t need. If you[photocopy] it, you haven’t chewed it.”
Sounds similar to Umberto Eco's admonition about photocopying: https://hypothes.is/a/U3Sg_r0ZEe25T2tD3U-nmw
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www.nybooks.com www.nybooks.com
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When he recorded his observations, he adhered to the Erasmian principle of distilling things down to their essence and entering them in notebooks, as if he were storing rare wine to be served for dégustation in future conversations.
This is quite similar to the advice by Sonke Ehrens and Nikolas Luhmann.
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- Nov 2023
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Gilmore, William J. Reading Becomes Necessity of Life: Material Cultural Life in Rural New England, 1780-1835. Knoxville: University of Tennessee Press, 1992. https://www.amazon.com/Reading-Becomes-Necessity-Life-1780-1835/dp/0870497685
ᔥ[[Dan Allosso]] in Darwin's Grandfather
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- Oct 2023
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Local file Local file
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LYNCH: No. I think a film is digested ideas andprocesses. If you take from things that have gonethrough that process, you’re further away from thesource. Ideas are the most important things. Andthey seem to be lying there in an ocean andavailable. So if you could go in and get your ownidea—now, it may have similarities to many thingsthat have gone before, but you feel it’s yours, andyou fall in love with it. And that’s a very goodfeeling.
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- Aug 2023
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americanhistory.si.edu americanhistory.si.edu
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The main thing I learned while reading through Phyllis Diller's jokes is that comedy has changed a lot since she started her career in the mid-1950s. Her comedy is focused on short one-liners that get laughs in quick succession, while today's comedy is more story-driven. Although a lot of her jokes are very time-bound due to their content, it was interesting to get a glimpse of what was happening at the time a joke was written. Each joke card has a date on it, and the cards span the 1960s to the 1990s. The topic of the jokes told a lot about what people were worried about or focused on at the time the joke was written, whether it was the inflation or student protests of the 1970s, a celebrity's many marriages, or gossip about the president at the time. While, like any comedian, some of her jokes fall flat, I appreciated Diller's hard work in meticulously recording, testing, and filing each joke in the gag file, along with her ability to make a joke about almost any topic.
evidence of comedy shift from 50s/60s of one liners to more story-based comedy of the 2000s onward. Some of this may come about through idea links or story links as seen in some of Diller's paperclipped cards (see https://hypothes.is/a/W9Wz-EXsEe6nZxew_8BUCg).
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www.si.edu www.si.edu
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BredenbeckCorp, Hanna. “The Evolution of Phyllis Diller’s Career in 7 Objects.” Smithsonian Institution (blog), March 13, 2017. https://www.si.edu/object/evolution-phyllis-dillers-career-7-objects%3Aposts_f0bf8f9142b02dbb88d3d720517f6697
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www.latimes.com www.latimes.com
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The joke file is a snapshot of American history.”
quote by Hanna BredenbeckCorp, of Smithsonian on Phyllis Diller's gag file
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- Jun 2023
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www.theatlantic.com www.theatlantic.com
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And because libraries generally do not take possession of the ebook files they rent from publishers, their crucial role as long-term preservers of culture has been severed from their role as institutions that provide democratic access—a striking change.
E-books have caused the missions of many libraries to shift away from institutions that provide democratic access to a preserved culture.
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- May 2023
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jillianhess.substack.com jillianhess.substack.com
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Write down all these slender ideas. It is surprising how often one sentence, jotted in a notebook, leads immediately to a second sentence. A plot can develop as you write notes. Close the notebook and think about it for a few days — and then presto! you’re ready to write a short story. — Patricia Highsmith: Her Diaries and Notebooks
quote is from Highsmith's Plotting and Writing Suspense Fiction
I love the concept of "slender ideas" as small, fleeting notes which might accumulate into something if written down. In saying "Close the notebook and think about it for a few days" Patricia Highsmith seems to be suggesting that one engage in diffuse thinking, passive digesting, or mulling rather than active or proactive thinking.
She also invokes the magic word "presto!" (which she exclaims) as if to indicate that magically the difficult work of writing is somehow no longer difficult. Many writers seem to indicate that this is a phenomenon, but never seem to put their finger on the mechanism of why it happens. Some seems to stem from the passive digestion over days with diffuse thinking, with portions may also stem from not starting from a blank page and having some material to work against instead of a vacuum.
From Patricia Highsmith: Her Diaries and Notebooks: 1941-1995 (Swiss Literary Archives)
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jillianhess.substack.com jillianhess.substack.com
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Besides the metaphor of the honeybee, Seneca also compares this method to a stomach digesting food as well as a choir that produces one sound from many harmonized voices.
In addition to the honeybee metaphor, Seneca compared note taking and collecting sententiae to a stomach digesting food and a choir producing a harmonized sound out of many voices.
(Sources from these last two? Potentially Epistle LXXXIV?)
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www.nytimes.com www.nytimes.com
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In her markings, Rose Caylor gave us a sense of her husband, the playwright Ben Hecht. In her copy of “A Child of the Century,” which Mr. Hecht wrote, she had drawn an arrow pointing to burns on a page. “Strikes matches on books,” she noted about her husband, who was a smoker.
This is a fascinating bit of reading practice.
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Not everyone values marginalia, said Paul Ruxin, a member of the Caxton Club. “If you think about the traditional view that the book is only about the text,” he said, “then this is kind of foolish, I suppose.”
A book can't only be about the text, it has to be about the reader's interaction with it and thoughts about it. Without these, the object has no value.
Annotations are the traces left behind of how one valued a book as they read and interacted with it.
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- Feb 2023
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forum.zettelkasten.de forum.zettelkasten.de
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reply https://forum.zettelkasten.de/discussion/comment/16622/#Comment_16622
Adler has an excellent primer on this subject that covers a lot of the basics in reasonable depth: - Adler, Mortimer J. “How to Mark a Book.” Saturday Review of Literature, July 6, 1940. (https://stevenson.ucsc.edu/academics/stevenson-college-core-courses/how-to-mark-a-book-1.pdf)
Marking books can be useful not only to the original reader, but future academics and historians studying material culture (eg: https://apps.lib.umich.edu/online-exhibits/exhibits/show/marks-in-books), and as @GeoEng51 indicates they might be shared by friends, family, romantic interests, or even perhaps all of the above (see: https://newcriterion.com/issues/2017/4/mrs-custers-tennyson).
For those interested in annotation marks and symbols (like @ctietze's "bolt" ↯) I outlined a few ideas this last month at: https://www.reddit.com/r/Zettelkasten/comments/10qw4l5/comment/j6vxn6a/?utm_source=reddit&utm_medium=web2x&context=3
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- Jan 2023
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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MATERIALS AND METHODS
What do you notice about the position of the method section in the paper? Is this the same as in the Report Writing Guidelines?
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www.cambridge.org www.cambridge.org
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Bacon, Bennett, Azadeh Khatiri, James Palmer, Tony Freeth, Paul Pettitt, and Robert Kentridge. “An Upper Palaeolithic Proto-Writing System and Phenological Calendar.” Cambridge Archaeological Journal, January 5, 2023, 1–19. https://doi.org/10.1017/S0959774322000415.
There may be questions as to whether or not this represents written language, but, if true, this certainly represents one of the oldest examples of annotation in human history!
cc: @remikalir
Tags
- material culture
- annotations
- indigenous knowledge
- writing as memory
- read
- orality
- cave art
- abstraction
- archaeoastronomy
Annotators
URL
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inst-fs-iad-prod.inscloudgate.net inst-fs-iad-prod.inscloudgate.net
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Course MaterialsThe assigned reading for this class is Just Mercy: A Story of Justice and Redemption by Bryan Stevenson.This text and other reading are available on Carmen.
Course materials.
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Local file Local file
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Another problem arises from the very nature of documentary material astexts not written for posterity. When reading Geniza letters, one is often in theposition of an uninvited guest at a social event, that is, someone who is unfa-miliar with the private codes and customs shared by the inner circle. Writersoften do not bother to explain themselves in a complete manner when they
know that the recipient is already familiar with the subject. 17
17 Indeed, writers often used this shared understanding to stress the relationship they had with the recipients.
Tags
Annotators
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- Oct 2022
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Local file Local file
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the author must not merely articulate his sources; he mustdigest them. A long passage quoted or closely followed "remainsan undigested bit of foreign matter." "Over quotation may meanunder thought."
relatable to: https://hypothes.is/a/wIyvtm0oEeyNypNtc--Clw
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www.sub.uni-hamburg.de www.sub.uni-hamburg.de
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Der Nachlass ist aber nicht nur ein wissenschaftshistorisches Dokument, sondern auch wegen der Rückseiten interessant: Jungius verwendete Predigttexte und Erbauungsliteratur, Schülermitschriften und alte Briefe als Notizpapier. Zudem wurde vieles im Nachlass belassen, was ihm irgendwann einmal zugeordnet wurde, darunter eine Reihe von Manuskripten fremder Hand, z. B. zur Astronomie des Nicolaus Reimers.
machine translation (Google):
The estate is not only a scientific-historical document, but also interesting because of the back: Jungius used sermon texts and devotional literature, school notes and old letters as note paper. In addition, much was left in the estate that was assigned to him at some point, including a number of manuscripts by someone else, e.g. B. to the astronomy of Nicolaus Reimers.
In addition to the inherent value of the notes which Jungius took and which present a snapshot of the state-of-the art of knowledge for his day, there is a secondary source of value as he took his notes on scraps of paper that represent sermon texts and devotional literature, school notes, and old letters. These represent their own historical value separate from his notes.
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Local file Local file
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one recognizes in the tactile realitythat so many of the cards are on flimsy copy paper, on the verge of disintegration with eachuse.
Deutsch used flimsy copy paper, much like Niklas Luhmann, and as a result some are on the verge of disintegration through use over time.
The wear of the paper here, however, is indicative of active use over time as well as potential care in use, a useful historical fact.
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- Aug 2022
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www.washingtonpost.com www.washingtonpost.com
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Hamblin, J. (2021, December 9). Perspective | Omicron won’t keep us from ending the pandemic. We’re doing that ourselves. Washington Post. https://www.washingtonpost.com/outlook/2021/12/09/omicron-coronavirus-individual-threat/
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regenesis.org.au regenesis.org.au
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the sacred is not some rarefied ‘other’, but completely embedded in the materiality of the world.
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www.google.com www.google.com
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I was doing some random searches for older material on zettelkasten in German and came across this.
Apparently I've come across this before in a similar context: https://hypothes.is/a/CsgyjAXQEeyMfoN7zLcs0w
The description now makes me want to read it all the more!
This is a book about a box that contained the world. The box was the Picture Academy for the Young, a popular encyclopedia in pictures invented by preacher-turned-publisher Johann Siegmund Stoy in eighteenth-century Germany. Children were expected to cut out the pictures from the Academy, glue them onto cards, and arrange those cards in ordered compartments—the whole world filed in a box of images.
As Anke te Heesen demonstrates, Stoy and his world in a box epitomized the Enlightenment concern with the creation and maintenance of an appropriate moral, intellectual, and social order. The box, and its images from nature, myth, and biblical history, were intended to teach children how to collect, store, and order knowledge. te Heesen compares the Academy with other aspects of Enlightenment material culture, such as commercial warehouses and natural history cabinets, to show how the kinds of collecting and ordering practices taught by the Academy shaped both the developing middle class in Germany and Enlightenment thought. The World in a Box, illustrated with a multitude of images of and from Stoy's Academy, offers a glimpse into a time when it was believed that knowledge could be contained and controlled.
Given the portions about knowledge and control, it might also be of interest to @remikalir wrt his coming book.
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chicagoreader.com chicagoreader.com
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“Bedrooms were the first MySpace. You came over to someone’s room, and it was all their music tastes and pictures of their friends.
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www.ischool.berkeley.edu www.ischool.berkeley.edu
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Historical Hypermedia: An Alternative History of the Semantic Web and Web 2.0 and Implications for e-Research. .mp3. Berkeley School of Information Regents’ Lecture. UC Berkeley School of Information, 2010. https://archive.org/details/podcast_uc-berkeley-school-informat_historical-hypermedia-an-alte_1000088371512. archive.org.
https://www.ischool.berkeley.edu/sites/default/files/audio/2010-10-20-vandenheuvel_0.mp3
Interface as Thing - book on Paul Otlet (not released, though he said he was working on it)
- W. Boyd Rayward 1994 expert on Otlet
- Otlet on annotation, visualization, of text
- TBL married internet and hypertext (ideas have sex)
- V. Bush As We May Think - crosslinks between microfilms, not in a computer context
- Ted Nelson 1965, hypermedia
t=540
- Michael Buckland book about machine developed by Emanuel Goldberg antecedent to memex
- Emanuel Goldberg and His Knowledge Machine: Information, Invention, and Political Forces (New Directions in Information Management) by Michael Buckland (Libraries Unlimited, (March 31, 2006)
- Otlet and Goldsmith were precursors as well
four figures in his research: - Patrick Gattis - biologist, architect, diagrams of knowledge, metaphorical use of architecture; classification - Paul Otlet, Brussels born - Wilhelm Ostwalt - nobel prize in chemistry - Otto Neurath, philosophher, designer of isotype
Paul Otlet
- wrote bibliography on law
- book: Something on Bibliography #wanttoread
- universal decimal classification system
- mundaneum
- Le Corbusier - architect worked with Otlet for building for Mundaneum; See: https://socks-studio.com/2019/05/05/the-shape-of-knowledge-the-mundaneum-by-paul-otlet-and-henri-la-fontaine/
Otlet was interested in both the physical as well as the intangible aspects of the Mundaneum including as an idea, an institution, method, body of work, building, and as a network.<br /> (#t=1020)
Early iPhone diagram?!?
(roughly) armchair to do the things in the web of life (Nelson quote) (get full quote and source for use) (circa 19:30)
compares Otlet to TBL
Michael Buckland 1991 <s>internet of things</s> coinage - did I hear this correctly? https://en.wikipedia.org/wiki/Internet_of_things lists different coinages
Turns out it was "information as thing"<br /> See: https://hypothes.is/a/kXIjaBaOEe2MEi8Fav6QsA
sugane brierre and otlet<br /> "everything can be in a document"<br /> importance of evidence
The idea of evidence implies a passiveness. For evidence to be useful then, one has to actively do something with it, use it for comparison or analysis with other facts, knowledge, or evidence for it to become useful.
transformation of sound into writing<br /> movement of pieces at will to create a new combination of facts - combinatorial creativity idea here. (circa 27:30 and again at 29:00)<br /> not just efficiency but improvement and purification of humanity
put things on system cards and put them into new orders<br /> breaking things down into smaller pieces, whether books or index cards....
Otlet doesn't use the word interfaces, but makes these with language and annotations that existed at the time. (32:00)
Otlet created diagrams and images to expand his ideas
Otlet used octagonal index cards to create extra edges to connect them together by topic. This created more complex trees of knowledge beyond the four sides of standard index cards. (diagram referenced, but not contained in the lecture)
Otlet is interested in the "materialization of knowledge": how to transfer idea into an object. (How does this related to mnemonic devices for daily use? How does it relate to broader material culture?)
Otlet inspired by work of Herbert Spencer
space an time are forms of thought, I hold myself that they are forms of things. (get full quote and source) from spencer influence of Plato's forms here?
Otlet visualization of information (38:20)
S. R. Ranganathan may have had these ideas about visualization too
atomization of knowledge; atomist approach 19th century examples:S. R. Ranganathan, Wilson, Otlet, Richardson, (atomic notes are NOT new either...) (39:40)
Otlet creates interfaces to the world - time with cyclic representation - space - moving cube along time and space axes as well as levels of detail - comparison to Ted Nelson and zoomable screens even though Ted Nelson didn't have screens, but simulated them in paper - globes
Katie Berner - semantic web; claims that reporting a scholarly result won't be a paper, but a nugget of information that links to other portions of the network of knowledge.<br /> (so not just one's own system, but the global commons system)
Mention of Open Annotation (Consortium) Collaboration:<br /> - Jane Hunter, University of Australia Brisbane & Queensland<br /> - Tim Cole, University of Urbana Champaign<br /> - Herbert Van de Sompel, Los Alamos National Laboratory annotations of various media<br /> see:<br /> - https://www.researchgate.net/publication/311366469_The_Open_Annotation_Collaboration_A_Data_Model_to_Support_Sharing_and_Interoperability_of_Scholarly_Annotations - http://www.openannotation.org/spec/core/20130205/index.html - http://www.openannotation.org/PhaseIII_Team.html
trust must be put into the system for it to work
coloration of the provenance of links goes back to Otlet (~52:00)
Creativity is the friction of the attention space at the moments when the structural blocks are grinding against one another the hardest. —Randall Collins (1998) The sociology of philosophers. Cambridge, MA: Harvard University Press (p.76)
Tags
- semantic web
- Mundaneum
- Wilhelm Ostwalt
- evidence
- idea links
- Open Annotation Collaboration
- W. Boyd Rayward
- hypermedia
- materialization of knowledge
- material culture
- Tim Cole
- listen
- Jane Hunter
- Emanuel Goldberg
- mnemonic devices
- atomic notes
- Universal Decimal Classification
- Otto Neurath
- Herbert Spencer
- S. R. Ranganathan
- index cards
- atomic ideas
- references
- Michael Buckland
- Le Corbusier
- Randall Collins
- Vannevar Bush
- Tim Berners-Lee
- Ted Nelson
- Paul Otlet
- Web 2.0
- memex
- atomist philosophy
- Herbert Van de Sompel
- octagonal index cards
- Charles van den Heuvel
- Hypothes.is
Annotators
URL
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www.levenger.com www.levenger.com
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Interesting piece of material culture hearkening back to an older analog era, but compatible with new digital technology (note the cut out for a power cord with use of a tablet or other digital reading/display device.)
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- Jul 2022
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education.nationalgeographic.org education.nationalgeographic.org
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When deposits of silt are compressed and the grains are pressed together, rocks such as siltstone form.
Siltstone is a potentially valuable by-product material from de-silting of riverbeds as means of flood aid.
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via3.hypothes.is via3.hypothes.is
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The Focus of Comprehension Questions
[35]
SUPER IMPORTANT
- Types of comprehension exercises/activities over comprehension questions. This has a big co-relation to the last paragraph of this page.
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At their easiest, such questions could beanswered by quoting parts of the text. These questions would bemore demanding if the learners were not allowed to look at the textwhile answering the questions.
Quoting answers is the easiest form of answering a questions. This means I should have more of these types of questions at low-level class.
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The Deliberate Study of Regular Correspondences and Rules
20.
Suggested teaching methods
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www.ifour.co www.ifour.co
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In this high-speed PCB design guide, we will encapsulate the high-speed PCB layout techniques, high-speed layout guidelines to help designers.
Would you like to speed up the performance of your product?
With innovative and fast electric equipment, designers and engineers can speed up the product. Not only this, you need a high speed PCB run faster.
Read the blog further to understand the rules and challenges of high-speed PCB design.
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www.wired.com www.wired.com
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At the time, BYD used lithium iron phosphate batteries
safer, longer life, less exotic materials, & what we seem to be switching back to.
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- Jun 2022
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hcommons.org hcommons.org
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https://hcommons.org/deposits/item/hc:33585/
See also Wiki created in combination with this course: https://digitalbookhistory.com/culturesofthebook/Main_Page
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digitalbookhistory.com digitalbookhistory.com
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https://digitalbookhistory.com/culturesofthebook/Main_Page
<small><cite class='h-cite via'>ᔥ <span class='p-author h-card'> whitney trettien </span> in whitney trettien on Twitter: "Curious about the development of different book technologies? The students in my "Cultures of the Book" seminar last semester made a wiki for you! Each entry is a short essay that uses examples from @upennlib. Open & free to use/remix: https://t.co/XN0C51MLrv" / Twitter (<time class='dt-published'>06/20/2022 01:10:21</time>)</cite></small>
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www.sas.ac.uk www.sas.ac.uk
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The course Marginalia in Books from Christopher Ohge is just crying out to have an annotated syllabus.
Wish I could follow along directly, but there's some excellent reference material hiding in the brief outline of the course.
Perhaps a list of interesting people here too for speaking at https://iannotate.org/ 2022 hiding in here? A session on the history of annotation and marginalia could be cool there.
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Archaeology of Reading project
https://archaeologyofreading.org/
The Archaeology of Reading in Early Modern Europe (AOR) uses digital technologies to enable the systematic exploration of the historical reading practices of Renaissance scholars nearly 450 years ago. This is possible through AOR’s corpus of thirty-six fully digitized and searchable versions of early printed books filled with tens of thousands of handwritten notes, left by two of the most dedicated readers of the early modern period: John Dee and Gabriel Harvey.
Perhaps some overlap here with: - Workshop in the History of Material Texts https://pennmaterialtexts.org/about/events/ - Book Traces https://booktraces.org via Andrew Stauffer, et al. - Schoenberg Institute's Coffe with a Codex https://schoenberginstitute.org/coffee-with-a-codex/ (perhaps to a lesser degree)
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https://www.sas.ac.uk/events/event/25322
<small><cite class='h-cite via'>ᔥ <span class='p-author h-card'>Jeremy Cherfas</span> (email) (<time class='dt-published'>06/16/2022 07:18:14</time>)</cite></small>
Tags
- Herman Melville
- digital humanities
- Workshop in the History of Material Texts
- W. B. Yeates
- annotated syllabus
- Christopher Ohge
- annotation
- book history
- annotation history
- read
- Book Traces
- marginalia
- annotations
- material culture
- Frank Fay
- archaeology
- courses on annotation
- reading practices
- IAnno
- Archaeology of Reading
- John Keates
- Mary Astell
- Hypothes.is
Annotators
URL
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The box makes me feel connected to a project. It is my soil. I feel this evenwhen I’ve back-burnered a project: I may have put the box away on a shelf, but Iknow it’s there. The project name on the box in bold black lettering is a constantreminder that I had an idea once and may come back to it very soon.
Having a physical note taking system also stands as a physical reminder and representation of one's work and focus. It may be somewhat out of the way on a shelf, but it takes up space in a way that digital files and notes do not. This invites one into using and maintaining it.
Link to - tying a string on one's finger as a reminder - method of loci - orality
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assets.pubpub.org assets.pubpub.org
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Discussion on
Bellinger C, Drozdyuk A, Crowley M, Tamblyn I. Balancing Information with Observation Costs in Deep Reinforcement Learning. Proceedings of the Canadian Conference on Artificial Intelligence [Internet]. 2022 May 27; Available from: https://caiac.pubpub.org/pub/0jmy7gpd
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- May 2022
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www.youtube.com www.youtube.com
- Apr 2022
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Local file Local file
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The bookitself participates in the history it recounts: it has a title page, table of contents,footnotes, a bibliography and an index to assist the reader, while the digitalcopy enables the reader to search for individual words and phrases as well asto copy-and-paste without disfiguring a material object.
Some scholars study annotations as part of material culture. Are they leaving out too much by solely studying those physically left in the books about which they were made, or should we instead also be looking at other sources like commonplace books, notebooks, note cards, digital spaces like e-readers that allow annotation, social media where texts are discussed, or even digital marginalia in services like Hypothes.is or Perusall?
Some of these forms of annotation allow a digital version of cut and paste which doesn't cause damage to the original text, which should be thought of as a good thing though it may separate the annotations from the original physical object.
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www.youtube.com www.youtube.com
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same with our with the with the dendrites we will always tell you the story tell the story to the juvenile who's coming through the novices who's coming through the ceremony will tell them so as they 00:47:47 get to a certain age or a certain time or a certain experience in the ceremony we will then pass that knowledge onto him and we'll take it to him so these hieroglyphs and 00:47:58 petroglyphs and the etchings on the rocks and the paintings on there on the cave walls that's our library that is our library
The dendroglyphs (markings on trees) or the petroglyphs (markings on stone in the stony territories) are the libraries of the indigenous peoples who always relate their stories from the markings back up to the sky.
via Uncle Ghillar Michael Anderson
Can this be linked to the practices of the Druids who may have had similar methods? How about linking the petroglyphs in the Celtic (English) countryside?
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- Mar 2022
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the going through abstraction and re-specification so i think i became interested in cetera carson also because i saw a lot of similarities 01:11:30 to what historians of science describe as experimental work in laboratories and that is especially in the field of science and technology 01:11:43 studies especially the work of hanzio greinberger he works for the max planck institute for history of science in berlin and the way he describes 01:11:55 um experimental work as a form of material deconstruction um is my blueprint for understanding 01:12:10 the work of lumen
Sönke Ahrens used Hans-Jörg Rheinberger's description of experimental work as a form of material deconstruction as a framework for looking at Niklas Luhmann.
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docdrop.org docdrop.org
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my article crap 00:23:20 detection 101 is a start I'm going to be working on expanding that into a book and a set of materials and I'm going to invite you and others to help me in 00:23:34 creating a public resource whereby strategies tools puzzles and tests that enable educators at all levels to teach 00:23:49 critical thinking critical consumption will be able to to provide support to anybody out there who wants to get the students started right 00:24:01 on the Internet
Rheingolds Internetseite checken - vllt finde ich hier etwas, das ich im krit. Denken Seminar und/oder für die Workshops/den Arbeitskreis nutzen kann
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mui.com mui.com
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onChange
The callback seems to receive 2 arguments. e and reactChild
In the past I got the selected value using e.target.value This seems to work.
Not sure of the use of the reactChild element that the callback receives.
Another important note regarding recording the recorded value of the select is noted in the "value" prop explanition ( below, on this very page )
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- Feb 2022
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pennmaterialtexts.org pennmaterialtexts.org
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https://pennmaterialtexts.org/homepage
How awesome looking is this? Note the regular online meetings/presentations and the backlog of videos on their YouTube channel: https://www.youtube.com/channel/UC8Ng6px3fgc4Yjw-en1GcsA
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mui.com mui.com
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Changing the semantic element
You might want to turn it into a span for any number of reasons. eg if you want one word in a sentence to be another color / style eg. I wear a red hat
<Typography component="div" > Contact Details : <Typography component="div" display="inline" variant="body2" color="black"> elroinoronha2@gmail.com </Typography> </Typography>
(copy it to a code editor for the code to make sense)
is a good solution since it defeats the "h1 cannot be a child of h1" DOM error + let us use the sx prop.
We would not be able to use sx prop to style the inline part if we would use the native
<span>
component ie. <Typography > yo mama <span> so fat </span> </Typography>You would have to use inline style styling instead.
Tags
Annotators
URL
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stackoverflow.com stackoverflow.com
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Another option (at least in v5) is to use the Box component and select the underlying html element to be img as given in the example below (copied from official docs for v5)
This
<Box/>
method is the preffered way of doing it. The<Paper/>
method too is a good idea. The basic reasoning is that only MUI components support thesx
prop ie. only they can fully use the MUI systems features. Thus, it doesnt make sense to use the native<img/>
component in the markup, as it does not support sx
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mui.com mui.com
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The SX prop does not work with non MUI components eg. native
<img/>
component. To style this, you will have to use the native inline styling ie.<img style=" ............". />
If you want to style the img and use some MUI system specific features, ie. you need to use sx ; do the following. Use <Box component="img" sx={{ }} /> instead. This supports the sx prop, thus your image will be customizable with sx prop.
Tags
Annotators
URL
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mui.com mui.com
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Color
The color prop does not support all MUI theme colors / their shades DIRECTLY through its color prop. Eg. The color prop supports primary but not primary.main/dark. It does not support the palette warning color at all.
To remedy this you have 2 options 1 BETTER -- give the icon the desired color through the sx prop OR 2. wrap the icon in a box and give the box to desired color
Tags
Annotators
URL
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www.perseus.tufts.edu www.perseus.tufts.edu
Tags
Annotators
URL
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de.wiktionary.org de.wiktionary.org
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Open Educational Resources: freie Lehr- und Lernmaterialien
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- Jan 2022
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en.wikipedia.org en.wikipedia.org
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Omake (御負け, usually written おまけ) means extra in Japanese.
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- Nov 2021
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site.pennpress.org site.pennpress.org
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https://site.pennpress.org/material-texts-2021/
Came across as the result of the Schoenberg Symposium on Manuscript Studies on 2021-11-17.
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site.pennpress.org site.pennpress.org
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https://site.pennpress.org/material-texts-2021/9780812224955/bitstreams/
Something about this seems related to the ideas of archiving and saving digital and physical culture.
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infohist.fas.harvard.edu infohist.fas.harvard.edu
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https://infohist.fas.harvard.edu/news/information-cultures-series-john-hopkins-university-press
This looks like a fascinating series and who could go wrong with Ann Blair, Anthony Grafton, and Earle Havens?
Also interesting to see what sorts of things they will find interesting at the cutting edge of all these disciplines.
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stackoverflow.com stackoverflow.com
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Honestly, I don't know. Strictly speaking it's different to a window being opened at a specific size (I can see the difference). I don't know whether that difference is material. Seeing as these are tests, I'd like to remove as many unknowns as possible. If someone can tell me that it makes no difference then I'd be happy.
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- Oct 2021
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material.io material.io
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Material is a design system – backed by open-source code – that helps teams build high-quality digital experiences.
Tags
Annotators
URL
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materializecss.com materializecss.com
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We did most of the heavy lifting for you to provide a default stylings that incorporate our custom components.
(The English here sounds awkward.)
Gyuri Lajos, in the Stop Reset Go team, recommended using Materialize CSS.
If it is based on Google’s Material Design, there are a lot of resources available to explore the possibilities. If I was building a Progressive Web App, this might be the place to start.
The project appears to be at an early stage of development, with a 1.0.0 release.
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materializecss.com materializecss.com
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Created and designed by Google, Material Design is a design language that combines the classic principles of successful design along with innovation and technology.
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themes.materializecss.com themes.materializecss.comGallery1
- Jul 2021
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journals.sagepub.com journals.sagepub.com
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Sheetal, A., Feng, Z., & Savani, K. (2020). Using Machine Learning to Generate Novel Hypotheses: Increasing Optimism About COVID-19 Makes People Less Willing to Justify Unethical Behaviors. Psychological Science, 31(10), 1222–1235. https://doi.org/10.1177/0956797620959594
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- May 2021
- Apr 2021
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material.io material.io
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medium.com medium.com
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Enclosed text fields with a rectangular (box) shape performed better than those with a line affordance
Tags
- answer the "why?"
- Material Design: text field
- text field
- form design
- component design
- learn from your mistakes
- visual design
- Material Design: text field: variant: line affordance only
- Material Design
- opportunity to improve/fix something
- constant evolution/improvement of software/practices/solutions
Annotators
URL
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material-ui.com material-ui.com
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Note: The standard variant of the TextField is no longer documented in the Material Design guidelines (here's why), but Material-UI will continue to support it.
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- Mar 2021
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journals.sagepub.com journals.sagepub.com
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Hoogeveen, S., Sarafoglou, A., & Wagenmakers, E.-J. (2020). Laypeople Can Predict Which Social-Science Studies Will Be Replicated Successfully: Advances in Methods and Practices in Psychological Science. https://doi.org/10.1177/2515245920919667
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- Feb 2021
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parsejournal.com parsejournal.comPARSE2
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Their modes of operating present an image of how thinking takes shape in and through material and embodied practice
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Knowles, however, does not make this point by means of an argument expressed in language, like Barad does, but by means of a material discursive formation
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- Jan 2021
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sveltematerialui.com sveltematerialui.com
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I guess this is what we would use for a popover, but why doesn't https://material.io/components/ (neither https://www.material.io/components/menus nor https://material.io/components/tooltips) say anything about popovers?
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www.material.io www.material.io
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The rail is a side navigation component that displays three to seven app destinations
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framework7.io framework7.io
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Open About Popover
I have to say, I like how it looks in their Apple and Desktop preview better than the Android/Material preview. I wish they had the arrow in Android Material too.
But on https://sveltematerialui.com/demo/menu-surface it doesn't bother me quite as much...
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medium.com medium.com
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In my opinion, it can sometimes look odd. Very interestingly, this is by design and is part of the Material design specification. This article isn’t to argue whether it should be this way or not, though; it’s just to change yours such that your MenuItem(s) show below the menu selection, like so:
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pixinvent.com pixinvent.com
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Material is the metaphor The metaphor of material defines the relationship between space and motion. The idea is that the technology is inspired by paper and ink and is utilized to facilitate creativity and innovation. Surfaces and edges provide familiar visual cues that allow users to quickly understand the technology beyond the physical world.
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material.io material.ioMenus1
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By default, menus open with an entrance animation. However, on desktop, menus can skip the animation and open instantly instead.
Why only on desktop? That delay drives me crazy. I would like to skip on web/mobile too.
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A notable difference between OS dialogs and Material dialogs is that OS dialogs save the work when a user dismisses a dialog but Material dialogs don’t.
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ux.stackexchange.com ux.stackexchange.com
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Material Design guidelines don't specify popovers, but the specification of material properties provide a clear set of properties that can be used to create popups.
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Material cannot occupy the same plane. But material can be stacked.
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material.io material.ioButtons3
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Outlined buttons are also a lower emphasis alternative to contained buttons, or a higher emphasis alternative to text buttons.
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Dialogs use text buttons because the absence of a container helps unify the action with the dialog text. Align text buttons to the right edge for left-to-right scripts.
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Text buttons are often embedded in contained components like cards and dialogs, in order to relate themselves to the component in which they appear. Because text buttons don’t have a container, they don’t distract from nearby content.
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- Dec 2020
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material.io material.ioDividers1
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material.io material.io
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medium.com medium.com
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getmdl.io getmdl.io
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github.com github.com
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github.com github.com
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I think the main difference between the two are the way API are served. Some smelte components need you to input big chunk of json as props, while i prefer keep props as primitive types and in the other hand give you different components tags to compose.
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github.com github.com
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Kanero, J., & Aktan-Erciyes, A. (2020). Family and language development during COVID-19: The case of Turkey. PsyArXiv. https://doi.org/10.31234/osf.io/n7k8z
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- Nov 2020
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uxdesign.cc uxdesign.cc
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I love the Material Design System’s buttons just because their principles are really well thought through.
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motion-software.com motion-software.com
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Before showing the final result of the .svelte components we will also add Material-UI, using the adapted version.
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www.plymouth.edu www.plymouth.edu
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packing
The process of putting genetic material and encapsulating it with a coat of proteins.
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material.io material.ioRally1
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material.io material.ioReply1
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material.io material.io
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Material is an adaptable system of guidelines, components, and tools that support the best practices of user interface design
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github.com github.com
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After a few hours experimenting (updated NPM, NODE, ...) I found that renaming _smui-theme.scss to smui-theme.scss (without underscore prefix) solved the problem. I don't understand the mechanics behind (why in documentation is file with prefix).
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github.com github.com
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material-ui.com material-ui.com
Tags
Annotators
URL
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- Oct 2020
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www.jasonhickel.org www.jasonhickel.org
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Detailliert und präzise weist @JasonHickel das Anti-Degrowth-Pamphlet von Andrew McAfee in Wired zurück.
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www.bejakovic.com www.bejakovic.com
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The 10 Commandments of A-list Copywriters
Tags
Annotators
URL
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- Sep 2020
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Local file Local file
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In this study, we focused on MgO ceramic doped with Tb as apotential dosimeter material. MgO ceramics have excellent thermaland mechanical properties with a high melting point (2800C). Upto now, MgO ceramics doped with several different transition andrare earth elements have been studied for dosimeters[17e21]
En este estudio, nos enfocamos en el cerámico de MgO dopado con Tb como un potencial material dosimétrico. . Los cerámicos de MgO tienen excelentes propiedades térmicas y mecánicas con un alto punto de fusión (2800°C). Hasta ahora, los cerámicos de MgO dopados con varios diferentes transiciones y elementos de tierras raras han sido estudiados para dosímetros.
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google.github.io google.github.io
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github.com github.com
Tags
Annotators
URL
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psycnet.apa.org psycnet.apa.org
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Moreau, D., & Gamble, B. (2020). Conducting a meta-analysis in the age of open science: Tools, tips, and practical recommendations. Psychological Methods, No Pagination Specified-No Pagination Specified. https://doi.org/10.1037/met0000351
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- Aug 2020
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psyarxiv.com psyarxiv.com
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Moreau, D., & Gamble, B. (2020). Conducting a Meta-Analysis in the Age of Open Science: Tools, Tips, and Practical Recommendations [Preprint]. PsyArXiv. https://doi.org/10.31234/osf.io/t5dwg
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mui-treasury.com mui-treasury.com
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I found that many people have bad experience when it comes to styling in Material-UI, so I want to help them overcome that point and see the beauty of it.
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- Jul 2020
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www.ox.ac.uk www.ox.ac.uk
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Oxford COVID-19 study: Face masks and coverings work – act now | University of Oxford. (n.d.). Retrieved 27 July 2020, from https://www.ox.ac.uk/news/2020-07-08-oxford-covid-19-study-face-masks-and-coverings-work-act-now
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material-ui.com material-ui.com
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www.excellentwebworld.com www.excellentwebworld.com
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Looking for effective design solutions? We create simple, clean, and attractive mobile app designs for business.
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- Apr 2020
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chiselapp.com chiselapp.com
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material complementario
¿Cómo se define materiales complementarios para obras que recurren a múltiples recursos, como los transmedia?
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www.psf.gov.pk www.psf.gov.pk
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PSF : Competitive Research Program. (n.d.). Retrieved April 9, 2020, from http://www.psf.gov.pk/crp.aspx
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- Feb 2020
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www.nature.com www.nature.com
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Fecal samples
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soiled toilet tissue
Tags
Annotators
URL
-
- Dec 2019
-
wellcomeopenresearch.org wellcomeopenresearch.org
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appropriately drafted material transfer agreement (MTA), as there is currently no legal requirement to that effect
Are there templates available for "appropriately drafted MTAs"?
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- Nov 2019
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codesandbox.io codesandbox.io
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stackoverflow.com stackoverflow.com
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Since the checkbox is rendering an input I would work with it rather than focusing on the image. You could do something like this: const checkbox = getByTestId('checkbox-1234').querySelector('input[type="checkbox"]') expect(checkbox).toHaveProperty('checked', true)
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the way Material UI works is it renders a different SVG when the checkbox is clicked, and not changing the attributes or anything on the actual input element. So how do I actually test that the element is checked in line with the react-testing-library philosophy?
These tags belong to entire page. This quote is just supporting evidence for the tags.
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github.com github.com
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Too bad I have to choose between Material Design (Material UI) and https://www.ag-grid.com (which has a lot more features).
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- Oct 2019
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foxhound87.github.io foxhound87.github.io
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iulian-radu-at.github.io iulian-radu-at.github.io
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teamwertarbyte.github.io teamwertarbyte.github.io
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mui.wertarbyte.com mui.wertarbyte.com
Tags
Annotators
URL
-
- Sep 2019
-
github.com github.com
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github.com github.com
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github.com github.com
- Aug 2019
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codesandbox.io codesandbox.io
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codesandbox.io codesandbox.io
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Example showing how to use inputRef on a <Select>
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www.npmjs.com www.npmjs.com
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material-ui.com material-ui.com
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Customization of the TextField can be cumbersome with the classes API, where you have to define the the classes prop. It's easier to use the default values, as described above. For example:
It's surprising that they show an example using styled-components, which is a competing style library to their own @material-ui/styles, and even admit that this might be preferred over using the classes API, which they admit may be cumbersome.
I like that they are open-minded enough to acknowledge that there are cases where built-in API may be too cumbersome, and even show examples of using styled-components.
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codesandbox.io codesandbox.io
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Demonstrates how label text will wrap at a point that appears to narrow when shrunk (the label can't even be as wide as the input it is labeling!), and how to work around this problem by adding styles:
'& label': { whiteSpace: 'nowrap' }
Of course, you would only want to do this if you are going to only be showing the label in shrunk state (which I think is safe to say is the case for date picker inputs), since it would look bad to actually have text overflowing outside of the input box. But if it's in "shrink" state, then it's actually above the input, so as long as there isn't another input/label directly to the right, and/or as long as we adjust the width so the right side of the label mostly lines up with the right side of the input, then I think we should be safe.
The input label "shrink" state isn't always correct. The input label is supposed to shrink as soon as the input is displaying something. In some circumstances, we can't determine the "shrink" state (number input, datetime input, Stripe input). You might notice an overlap.
To workaround the issue, you can force the "shrink" state of the label.
You need to make sure that the input is larger than the label to display correctly.
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codesandbox.io codesandbox.io
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codesandbox.io codesandbox.io
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github.com github.com
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@material-ui/core/Select
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codesandbox.io codesandbox.io
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material-ui.com material-ui.com
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material.angularjs.org material.angularjs.org
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material-components.github.io material-components.github.io
- May 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Transformation of calcium-competent cells was carried out by the procedure detailed below: •The competent bacterial cells were thawed briefly and 200 μL of cells was mixed rapidly with plasmid DNA (10-50 ng) in fresh, sterile microcentrifuge tubes and maintained on ice for 30 min. A negative control with competent cells only (no added DNA) was also included. •Cell membranes were disrupted by subjecting cells to heat-pulse (42 °C) for 90 sec. •After heat shock, cells were incubated on ice for 5 min. •Cells were then mixed with 1 mL LB medium and incubated with shaking at 37 °C for 1 h. •For blue/white screening 40 μL of X-gal solution (20 mg mL-1 in dimethylformamide) and 4 μL of the IPTG (200 mg mL-1) was spread on LB-ampicillin (LB-amp) plates with a sterile glass rod. The plate was allowed to dry for 1h at 37 °C prior to spreading of bacterial cells. •Bacterial cells (100-200 μL) were spread and the plate was incubated at 37 °C for overnight. •White colonies were picked from the plates and suspended into LB-amp broth and cultivated to OD600=0.5
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2 mL of an overnight culture of E. coli cells was inoculated into 100 mL LB medium and incubated with vigorous shaking at 30 °C until A600 of 0.8 was reached. •Cells were collected in 50 mL plastic (Falcon) tubes, cooled for 15 min on ice and centrifuged in a pre-cooled centrifuge (4,000 rpm for 10 min at 4 °C). •The pellet was suspended in 20 mL of ice-cold 50 mM CaCl2-15% glycerol solution, maintained on ice for 15 min and centrifuged again at 4,000 rpm for 10 min at 4 °C. •Pellet was resuspended in 2 mL of ice-cold 50 mM CaCl2-15 % glycerol solution, kept on ice for 30 min and aliquoted in 400 μL in microcentrifuge tubes. These were stored at -80 °C until required.
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Preparation of electrocompetent cells (E. coli cells) A protocol was employed. The procedure was carried out in cold under sterile conditions as follows: •A single colony of E. coli DH10B/ DH5α/XL1blue was inoculated in 20 mL of LB medium and grown overnight at 30 °C. •500 mL LB medium was inoculated with 5mL of this overnight grown culture of the E. coli and incubated with vigorous shaking (250 rpm) at 30 °C until an A600of 0.5 - 0.8 was achieved. •The cells were chilled in ice for 10-15 min and transferred to prechilled Sorvall® centrifuge tubes and sedimented at 4,000 rpm for 20 min at 4 °C. •The supernatant was decanted and cells were resuspended in 500 mL of sterile ice-cold water, mixed well and centrifuged as described above. •The washing of the cells described above was repeated with 250 mL of sterile ice-cold water, following which cells were washed with 40 mL of ice-cold 10 % (v/v) glycerol and centrifuged at 4,000 rpm for 10 min. •The glycerol solution was decanted and the cell volume was recorded. The cells were resuspended in an equal volume of ice-cold 10 % glycerol. •Cells were then dispensed in 40 μL volumes and stored at -80 °C until required.
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In order to minimize self ligation of vector during cloning experiments, the digested DNA was subsequently treated with calf intestinal phosphatase (CIP) [NEB, UK]. The reaction conditions and amount of CIP were optimized and varied from (0.06-1) unit/picomole DNA termini. The dephosphorylation reaction was carried out in 50 μL reaction as follows. Reaction mixture containing no restriction enzyme was treated as control. Reaction was incubated for 1 h at 37 °C and stopped by heat inactivation at 65 °C for 20 min. 2.5.5. Composition of restriction mixture (50 μL) Linearized Plasmid DNA X μL (1 μg) CIP 1 μL (0.06-1 U μL-1) Reaction buffer (10X) 5.0 μL Distilled water Y μL Total volume 50 μL Linearized and dephosphorylated plasmids from each reaction were purified from low melting agarose gel using gel extraction method according to the manufacturer’s protocol (Qiagen gel extraction kit, Germany). 100 ng DNA from each reaction was then ligated in15 μL reaction volume containing 1.5 μL of 10X ligation buffer (NEB, England) and 0.2 μL of T4 DNA ligase to check the efficiency of self ligation after dephosphoryaltion. The ligation mixture was incubated at 16 °C for overnight and transformed into E. coli DH5αcompetent cells.
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The isolated DNA was diluted (1:100) with MQ. The concentration (mg mL-1) of the DNA [N] was determined spectrophotometrically by recording absorbance at 260 nm (A260) as: A260 = ε 260[N]where ε 260 is the extinction coefficient of DNA (50 for ds DNA) [N] = concentration (mg mL-1) of DNA The concentration of ds DNA [N] was calculated as [DNA] (mg mL-1) = A260/ε 260 [DNA] (μg mL-1) = A260 × 50 × dilution factor Purity of DNA was checked by measuring absorbance at 260 and 280 nm and calculating the A260/A280 ratio (Sambrook et al., 1989). A DNA sample was considered pure when A260/A280 ranged between 1.8-1.9. An A260/A280 < 1.7 indicated contamination of the DNA preparation with protein or aromatic substances such as phenol, while an A260/A230 < 2.0 indicated possible contamination of high molecular weight polyphenolic compounds like humic substances.
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Determination of DNA quantity and purity
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as well as commercial methods (MN kit, Germany; Mo-Bio kit, CA, USA; Zymo soil DNA kit, CA, USA) according to the manufacturer’s protocols and compared in terms of DNA yield and purity.
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The soil DNA from Pantnagar and Lonar soil samples were also extracted by various manual (Desai and Madamwar, 2007; Agarwal et al., 2001; Yamamoto et al., 1998
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Alternatively metagenomic DNA was extracted from the alkaline soil samples by using different commercial kits (UltraClean™, PowerSoil™ [Mo Bio Laboratories Inc., Carlsbad, CA, USA], Nucleospin kit [Macherey-Nagal, Germany] and Zymo soil DNA isolation kit [CA, USA]). The DNA was finally suspended in 100 μL of sterile Milli Q water for further analysis.
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Commercial kits
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Comparison of yield and purity of crude DNA
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Soil (1 gm) was suspended with 0.4 gm (w/w) polyactivated charcoal (Datta and Madamwar, 2006) and 20 μL proteinase K (10 mg mL-1) in 2 mL of modified extraction buffer [N,N,N,N cetyltrimethylammonium bromide (CTAB) 1% w/v, polyvinylpolypyrrolidone (PVPP) 2% w/v, 1.5 M NaCl, 100mM EDTA, 0.1 M TE buffer (pH 8.0), 0.1M sodium phosphate buffer (pH 8.0) and 100 μL RNaseA] [Zhou et al., 1996] in 20 mL centrifuge tubes to homogenize the sample and incubated at 37 °C for 15 min in an incubator shaker at 200 rpm. Subsequently, 200 μL of 10% SDS was added to the homogenate and kept at 60 °C for 2 h with intermittent shaking. DNA was precipitated by adding 0.5 V PEG 8000 (30 % in 1.6 M NaCl) and left at room temperature for an hour (Yeates et al., 1998). The precipitated DNA was collected by centrifugation at 8000 x g at 4 °C. The supernatant was discarded and pellet was dissolved in 1 mL of TE buffer (pH 8.0) and then100 μL of 5 M potassium acetate (pH 4.5) was added and incubated at 4 °C for 15 min. The supernatant was collected after centrifugation at 8000 x g and treated with equal volumes of phenol: chloroform (1:1) followed by chloroform: isoamylalcohol (24:1) at 8000 x g for 15 min
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PROTOCOL FOR OPTIMIZATION OF HUMIC ACID-FREE DNA FROM ALKALINE SOILS
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Various strains of Escherchia coli (DH5α, XL1Blue, DH10B) were used as hosts for the propagation of recombinant vectors. In addition, Bacillus subtilis was used as a host for the expression of xylanase gene from the recombinant vector pWHMxyl. Different vectors used in this investigation are listed in
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BACTERIAL STRAINS
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