the lowest copy number sample points impacted faster and sronger, then you just need to stabilize by adding a neutral nucleic acid background in your standard curve. I usually use water containig 10ng/microl yeast tRNA to perform serial dilutions. This will first create a reaction background similar to your RTQPCR reaction, but also stabilize your DNA copies; I can freeze and thaw (min 20C) more than 50 times the same standard curve sample without any loss in Cts, from 10E6 to 10E2 copies. When I tested the same standard curve but diluted in water only, then the 10E2 started to be slightly affected after one freeze and thaw and then crashed further; then higher copy numbers samples were also affected after 2 to 3 freeze and thaw.

Slightly on a tangent, but https://github.com/hypothesis/h/issues/777 could be a good target for https://solidproject.org/ to address. The Web Annotation Vocabulary is defined in RDF, so there should be zero overhead.

Once running, kaniko will then get the data from STDIN and create the build context as a compressed tar. It will then unpack the compressed tar of the build context before starting the image build.

Pipes are great for taking output of one command and transforming it using other commands like jq. They’re a key part of the Unix philosophy of “small sharp tools”: since commands can be chained together with pipes, each command only needs to do one thing and then hand it off to another command.

Write programs to handle text streams, because that is a universal interface.

Some variety of markdown support the following syntax, but not supported in Hugo/BlackFriday. [Lua Software](http://www.luasoftware.com){:target="_blank"}

[link](url){:target="_blank"} Works for jekyll or more specifically kramdown, which is a superset of markdown, as part of Jekyll's (default) configuration. But not for plain markdown. ^_^

For ghost markdown use: [Google](https://google.com" target="_blank) Found it here: https://cmatskas.com/open-external-links-in-a-new-window-ghost/

The aim of this list is to document all Markdown syntax variations (rather than implementations).

Rails also adds a test method that takes a test name and a block. It generates a normal Minitest::Unit test with method names prefixed with test_. So you don't have to worry about naming the methods, and you can write something like:

a defined quantity of a nonhuman control virus (seal herpes virus [SHV], kindly provided by H. G. M. Niesters, University of Rotterdam, The Netherlands) was spiked into each sample prior to DNA and RNA extraction. Since constant DNA quantities of the control virus are coextracted even when RNA isolation kits are used (H. G. M. Niesters, personal communication; our own unpublished observations), the virus can also serve as a control in RNA virus detection assays.

In many households, telephones were hard-wired to wall terminals before connectors like RJ11 and BS 6312 became standardized.

We will use the Open Annotation specs as the basis for a more formal data model standard.

The standard error (the standard deviation of the distribution of sample means) has this formula:

The Web Annotation Data Model specification describes a structured model and format to enable annotations to be shared and reused across different hardware and software platforms.

Due dates and threshold dates aren't part of the todo.txt format spec! Due date and threshold date are implemented as key:value tags, which are fully compliant with the todo.txt format spec. They both are popular extensions to the todo.txt command line scripts.

*17.08

uBlock Origin blocks ads through its support of the Adblock Plus filter syntax. uBlock Origin extends the syntax and is designed to work with custom rules and filters.

TC39 urges caution when using Stage 2-or below proposals, as it might result in inadvertent pressure from the community to keep the implementation as-is instead of improving it for fear of breaking existing code or ecosystem fragmentation (e.g. using a different symbol like # instead of @ for decorators).

The reasoning is that saying "ES7 Decorators" assumes that Decorators is expected to be in ES7. I mentioned this in my last post regarding compiling node_modules, but being in a particular Stage doesn't guarantee much: a proposal can stall, move backward, or get removed entirely.

5 nM fluorescein in 0.01 M NaOH was measured in black 96-well microplates

enquo() uses some dark magic to look at the argument, see what the user typed, and return that value as a quosure

Epictetus wrote nothing; and all that we have under his name was written by an affectionate pupil, Arrian, afterwards the historian of Alexander the Great, who, as he tells us, took down in writing the philosopher’s discourses

NDEx lets you specify Licenses and Request DOIs for your networks to include in grant proposals or publications thus enabling papers to link directly to your data.

Require DOI?: Set this to true in order to generate a permanent identifier for this collection (Please set this to true for collections related to publications).

The use of the common metabolite names in the RefMet database is strongly encouraged in order to be able to compare and contrast metabolite data across different experiments and studies.

the ANZI z535 standard for admonitions

Thirdly, When the signification of the word is referred to a standard, which standard is not easy to be known.

Coming back to the two ‘FreeSync’ settings in the monitor OSD, they differ in the variable refresh rate range that they support. ‘Standard Engine’ supports 90 – 144Hz (90 – 119Hz via HDMI) whilst ‘Ultimate Engine’ gives a broader variable refresh rate range of 70 – 144Hz (62 – 119Hz via HDMI). We didn’t notice any adverse effects when using ‘Ultimate Engine’, so we’d suggest users simply stick to that option.

Classifications may or may not become standardized. If they do not, they are ad hoc, limited to an individual or a local community, and/or of limited duration. At the same time, every successful standard imposes a classification system, at the very least between good and bad ways of organizing actions or things. And the work-arounds involved in the practical use of standards frequently entail the use of ad hoc nonstandard categories.

Standards Classifications and standards are closely related, but not identical. While this book focuses on classification, standards are crucial compo­nents of the larger argument. The systems we discuss often do become standardized; in addition, a standard is in part a way of classifying the world.

If ever there were a newspaper headline custom-made for Jay Leno's monologue, this was it. Kids taking on McDonald's this week, suing the company for making them fat. Isn't that like middle-aged men suing Porsche for making them get speeding tickets? Whatever happened to personal responsibility?

THE ISLAMIC REPUBLIC OF IRAN HAS BECOME, in two senses,an extraordinary preoccupation of the United States.

The standardization of local times into standard world time is one of the prime examples for the push towards standardization and integration also on the temporal scale (Zerubavel, 1982).

Despite the apparent diversity of themes, certain common patterns can be discerned in empirical studies dealing with time. They bear the imprint of the ups and downs of research fashions as well as the waxing and waning of influences from neighbouring disciplines. But they all acknowledge 'time as a problem' in 'time-compact' societies (Lenntorp, 1978), imbued with the pressures of time that come from time being a scarce resource.

global technology firm with social and economic justice at its very core

The jōyō kanji (常用漢字, regular-use kanji) are 2,136 characters consisting of all the Kyōiku kanji, plus 1,130 additional kanji taught in junior high and high school[9].

Some characters were given simplified glyphs, called shinjitai (新字体). Many variant forms of characters and obscure alternatives for common characters were officially discouraged.

This was in part because of the considerable time that it took WWARN secretariat staff to persuade Oxford University lawyers that seven pages of often arcane legal language could be streamlined into a three-page document in plain English, understandable to malaria researchers worldwide.

REQUIRED fields are no longer supported in Standard SQL. If you're using Standard SQL (as opposed to Legacy SQL), they recommend you change all your REQUIRED fields to NULLABLE.

Common

Paleo

There has been ferment among the literati since Bob Dylan was awarded the Nobel Prize in literature. Many say that however well Dylan does what he does, it is not literature. Dylan did not go to Stockholm on Saturday to collect his prize, which the Swedish Academy says was awarded “for having created new poetic expressions within the great American song tradition.”

In Silicon Valley, where I live, the word “disruption” has an overwhelmingly positive valence: Thousands of smart, young people arrive here every year hoping to disrupt established ways of doing business — and become very rich in the process.For almost everyone else, however, disruption is a bad thing.

and to be as little Negro and as much American as possible.

to follow the pattern that G&B presents

MCF18 (Meta Content Framework)